Restriction map for
pF1KSDA1627
(Linear) (Six-base) MAPSORT of: KIAA1627.seq Check: 482 from: 1 to: 1368
KIAA1627.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 371 1368
Size: 371 997
AflII C'TTAA_G
Cuts at: 0 319 1368
Size: 319 1049
AleI CACnn'nnGTG
Cuts at: 0 1349 1368
Size: 1349 19
ApoI r'AATT_y
Cuts at: 0 273 393 553 986 1368
Size: 273 120 160 433 382
Fragments arranged by size:
433 382 273 160 120
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 178 211 1368
Size: 178 33 1157
Fragments arranged by size:
1157 178 33
BanI G'GyrC_C
Cuts at: 0 94 1368
Size: 94 1274
BanII G_rGCy'C
Cuts at: 0 455 1236 1368
Size: 455 781 132
Fragments arranged by size:
781 455 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 817 1029 1368
Size: 227 590 212 339
Fragments arranged by size:
590 339 227 212
BfrBI ATG'CAT
Cuts at: 0 1123 1368
Size: 1123 245
BglII A'GATC_T
Cuts at: 0 1291 1368
Size: 1291 77
BsaHI Gr'CG_yC
Cuts at: 0 1026 1368
Size: 1026 342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 159 189 1368
Size: 159 30 1179
Fragments arranged by size:
1179 159 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 485 1190 1238 1319 1352 1368
Size: 43 442 705 48 81 33 16
Fragments arranged by size:
705 442 81 48 43 33 16
BsiHKAI G_wGCw'C
Cuts at: 0 455 1140 1236 1344 1368
Size: 455 685 96 108 24
Fragments arranged by size:
685 455 108 96 24
Bsp1286I G_dGCh'C
Cuts at: 0 455 1140 1236 1344 1368
Size: 455 685 96 108 24
Fragments arranged by size:
685 455 108 96 24
BsrBI CCG'CTC
Cuts at: 0 30 1368
Size: 30 1338
BsrGI T'GTAC_A
Cuts at: 0 1162 1368
Size: 1162 206
BstYI r'GATC_y
Cuts at: 0 20 825 1291 1368
Size: 20 805 466 77
Fragments arranged by size:
805 466 77 20
BtgI C'CryG_G
Cuts at: 0 1272 1332 1368
Size: 1272 60 36
Fragments arranged by size:
1272 60 36
BtsI GCAGTG_nn'
Cuts at: 0 611 858 1368
Size: 611 247 510
Fragments arranged by size:
611 510 247
EaeI y'GGCC_r
Cuts at: 0 1269 1368
Size: 1269 99
EarI CTCTTCn'nnn_
Cuts at: 0 284 726 1038 1245 1368
Size: 284 442 312 207 123
Fragments arranged by size:
442 312 284 207 123
EcoICRI GAG'CTC
Cuts at: 0 453 1234 1368
Size: 453 781 134
Fragments arranged by size:
781 453 134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 164 855 1368
Size: 164 691 513
Fragments arranged by size:
691 513 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 324 356 810 842 1368
Size: 38 32 254 32 454 32 526
Fragments arranged by size:
526 454 254 38 32 32 32
HincII GTy'rAC
Cuts at: 0 927 1368
Size: 927 441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 760 1059 1368
Size: 701 59 299 309
Fragments arranged by size:
701 309 299 59
MslI CAynn'nnrTG
Cuts at: 0 920 1349 1368
Size: 920 429 19
Fragments arranged by size:
920 429 19
NdeI CA'TA_TG
Cuts at: 0 282 1119 1368
Size: 282 837 249
Fragments arranged by size:
837 282 249
NsiI A_TGCA'T
Cuts at: 0 1125 1368
Size: 1125 243
PfoI T'CCnGG_A
Cuts at: 0 23 1368
Size: 23 1345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 848 880 1368
Size: 848 32 488
Fragments arranged by size:
848 488 32
SacI G_AGCT'C
Cuts at: 0 455 1236 1368
Size: 455 781 132
Fragments arranged by size:
781 455 132
ScaI AGT'ACT
Cuts at: 0 312 1368
Size: 312 1056
SfcI C'TryA_G
Cuts at: 0 980 1368
Size: 980 388
SmlI C'TyrA_G
Cuts at: 0 319 1368
Size: 319 1049
SspI AAT'ATT
Cuts at: 0 592 1368
Size: 592 776
StyI C'CwwG_G
Cuts at: 0 726 1368
Size: 726 642
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 564 1235 1368
Size: 564 671 133
Fragments arranged by size:
671 564 133
TatI w'GTAC_w
Cuts at: 0 310 503 1051 1162 1368
Size: 310 193 548 111 206
Fragments arranged by size:
548 310 206 193 111
XmnI GAAnn'nnTTC
Cuts at: 0 989 1368
Size: 989 379
Enzymes that do cut:
AccI AflII AleI ApoI BaeI BanI BanII BbsI
BfrBI BglII BsaHI BsaXI BseRI BsiHKAI Bsp1286I BsrBI
BsrGI BstYI BtgI BtsI EaeI EarI EcoICRI FalI
Hin4I HincII MmeI MslI NdeI NsiI PfoI PpiI
SacI ScaI SfcI SmlI SspI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PflMI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI