Restriction map for
pF1KA1624
(Linear) (Six-base) MAPSORT of: KIAA1624.seq Check: 7288 from: 1 to: 1800
KIAA1624.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 1220 1800
Size: 1220 580
Acc65I G'GTAC_C
Cuts at: 0 415 1800
Size: 415 1385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 423 479 1309 1631 1800
Size: 257 166 56 830 322 169
Fragments arranged by size:
830 322 257 169 166 56
AlwNI CAG_nnn'CTG
Cuts at: 0 96 412 1134 1587 1800
Size: 96 316 722 453 213
Fragments arranged by size:
722 453 316 213 96
ApoI r'AATT_y
Cuts at: 0 776 1633 1800
Size: 776 857 167
Fragments arranged by size:
857 776 167
AvaI C'yCGr_G
Cuts at: 0 1379 1800
Size: 1379 421
AvrII C'CTAG_G
Cuts at: 0 43 1800
Size: 43 1757
BanI G'GyrC_C
Cuts at: 0 12 415 1237 1792 1800
Size: 12 403 822 555 8
Fragments arranged by size:
822 555 403 12 8
BanII G_rGCy'C
Cuts at: 0 1775 1800
Size: 1775 25
BbeI G_GCGC'C
Cuts at: 0 16 1796 1800
Size: 16 1780 4
Fragments arranged by size:
1780 16 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 523 1602 1691 1800
Size: 245 278 1079 89 109
Fragments arranged by size:
1079 278 245 109 89
BglI GCCn_nnn'nGGC
Cuts at: 0 10 1800
Size: 10 1790
BglII A'GATC_T
Cuts at: 0 1103 1800
Size: 1103 697
Bme1580I G_kGCm'C
Cuts at: 0 1180 1800
Size: 1180 620
BmrI ACTGGGnnnn_n'
Cuts at: 0 414 1246 1800
Size: 414 832 554
Fragments arranged by size:
832 554 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1111 1800
Size: 360 751 689
Fragments arranged by size:
751 689 360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1800
Size: 121 1679
BsaBI GATnn'nnATC
Cuts at: 0 844 1800
Size: 844 956
BsaHI Gr'CG_yC
Cuts at: 0 13 1793 1800
Size: 13 1780 7
Fragments arranged by size:
1780 13 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 940 943 970 973 1800
Size: 940 3 27 3 827
Fragments arranged by size:
940 827 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 948 1186 1531 1800
Size: 948 238 345 269
Fragments arranged by size:
948 345 269 238
BseYI C'CCAG_C
Cuts at: 0 78 983 1061 1438 1800
Size: 78 905 78 377 362
Fragments arranged by size:
905 377 362 78 78
Bsp1286I G_dGCh'C
Cuts at: 0 1180 1775 1800
Size: 1180 595 25
Fragments arranged by size:
1180 595 25
BspHI T'CATG_A
Cuts at: 0 1109 1800
Size: 1109 691
BsrBI CCG'CTC
Cuts at: 0 7 1800
Size: 7 1793
BsrDI GCAATG_nn'
Cuts at: 0 1040 1529 1800
Size: 1040 489 271
Fragments arranged by size:
1040 489 271
BstBI TT'CG_AA
Cuts at: 0 852 1800
Size: 852 948
BstEII G'GTnAC_C
Cuts at: 0 1752 1800
Size: 1752 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1259 1438 1565 1800
Size: 1259 179 127 235
Fragments arranged by size:
1259 235 179 127
BstYI r'GATC_y
Cuts at: 0 1103 1800
Size: 1103 697
BtgI C'CryG_G
Cuts at: 0 36 53 566 1766 1800
Size: 36 17 513 1200 34
Fragments arranged by size:
1200 513 36 34 17
BtsI GCAGTG_nn'
Cuts at: 0 614 1800
Size: 614 1186
DraI TTT'AAA
Cuts at: 0 332 876 1800
Size: 332 544 924
Fragments arranged by size:
924 544 332
EaeI y'GGCC_r
Cuts at: 0 2 50 98 1598 1800
Size: 2 48 48 1500 202
Fragments arranged by size:
1500 202 48 48 2
EarI CTCTTCn'nnn_
Cuts at: 0 1036 1252 1328 1800
Size: 1036 216 76 472
Fragments arranged by size:
1036 472 216 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 360 423 479 1111 1309 1631 1800
Size: 257 103 63 56 632 198 322 169
Fragments arranged by size:
632 322 257 198 169 103 63 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 977 1800
Size: 977 823
EcoO109I rG'GnC_Cy
Cuts at: 0 1131 1318 1800
Size: 1131 187 482
Fragments arranged by size:
1131 482 187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 673 1800
Size: 453 220 1127
Fragments arranged by size:
1127 453 220
HaeII r_GCGC'y
Cuts at: 0 16 1027 1796 1800
Size: 16 1011 769 4
Fragments arranged by size:
1011 769 16 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1323 1355 1800
Size: 1323 32 445
Fragments arranged by size:
1323 445 32
HincII GTy'rAC
Cuts at: 0 486 1221 1800
Size: 486 735 579
Fragments arranged by size:
735 579 486
HpaI GTT'AAC
Cuts at: 0 486 1800
Size: 486 1314
KasI G'GCGC_C
Cuts at: 0 12 1792 1800
Size: 12 1780 8
Fragments arranged by size:
1780 12 8
KpnI G_GTAC'C
Cuts at: 0 419 1800
Size: 419 1381
MfeI C'AATT_G
Cuts at: 0 938 1800
Size: 938 862
MslI CAynn'nnrTG
Cuts at: 0 202 596 802 1563 1800
Size: 202 394 206 761 237
Fragments arranged by size:
761 394 237 206 202
MspA1I CmG'CkG
Cuts at: 0 38 55 1450 1800
Size: 38 17 1395 350
Fragments arranged by size:
1395 350 38 17
NarI GG'CG_CC
Cuts at: 0 13 1793 1800
Size: 13 1780 7
Fragments arranged by size:
1780 13 7
NcoI C'CATG_G
Cuts at: 0 566 1800
Size: 566 1234
PasI CC'CwG_GG
Cuts at: 0 963 1776 1800
Size: 963 813 24
Fragments arranged by size:
963 813 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 129 412 1134 1587 1800
Size: 129 283 722 453 213
Fragments arranged by size:
722 453 283 213 129
PfoI T'CCnGG_A
Cuts at: 0 1586 1800
Size: 1586 214
PpuMI rG'GwC_Cy
Cuts at: 0 1131 1318 1800
Size: 1131 187 482
Fragments arranged by size:
1131 482 187
PstI C_TGCA'G
Cuts at: 0 92 1800
Size: 92 1708
PvuII CAG'CTG
Cuts at: 0 1450 1800
Size: 1450 350
SacII CC_GC'GG
Cuts at: 0 39 56 1800
Size: 39 17 1744
Fragments arranged by size:
1744 39 17
SalI G'TCGA_C
Cuts at: 0 1219 1800
Size: 1219 581
SfcI C'TryA_G
Cuts at: 0 88 1800
Size: 88 1712
SfoI GGC'GCC
Cuts at: 0 14 1794 1800
Size: 14 1780 6
Fragments arranged by size:
1780 14 6
SmaI CCC'GGG
Cuts at: 0 1381 1800
Size: 1381 419
SmlI C'TyrA_G
Cuts at: 0 136 1800
Size: 136 1664
SrfI GCCC'GGGC
Cuts at: 0 1381 1800
Size: 1381 419
StuI AGG'CCT
Cuts at: 0 656 1800
Size: 656 1144
StyI C'CwwG_G
Cuts at: 0 43 459 566 684 923 1800
Size: 43 416 107 118 239 877
Fragments arranged by size:
877 416 239 118 107 43
TatI w'GTAC_w
Cuts at: 0 547 995 1800
Size: 547 448 805
Fragments arranged by size:
805 547 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 940 972 1115 1147 1800
Size: 940 32 143 32 653
Fragments arranged by size:
940 653 143 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1764 1800
Size: 1764 36
XmaI C'CCGG_G
Cuts at: 0 1379 1800
Size: 1379 421
XmnI GAAnn'nnTTC
Cuts at: 0 1477 1800
Size: 1477 323
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApoI AvaI AvrII BanI
BanII BbeI BbsI BglI BglII Bme1580I BmrI BpmI
BpuEI BsaBI BsaHI BsaXI BseRI BseYI Bsp1286I BspHI
BsrBI BsrDI BstBI BstEII BstXI BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MslI MspA1I
NarI NcoI PasI PflMI PfoI PpuMI PstI PvuII
SacII SalI SfcI SfoI SmaI SmlI SrfI StuI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaWI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI TaqII Tth111I XbaI XhoI ZraI