Restriction map for
pF1KSDA1623
(Linear) (Six-base) MAPSORT of: KIAA1623.seq Check: 3300 from: 1 to: 3696
KIAA1623.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 609 1619 3696
Size: 609 1010 2077
Fragments arranged by size:
2077 1010 609
AccI GT'mk_AC
Cuts at: 0 779 883 3696
Size: 779 104 2813
Fragments arranged by size:
2813 779 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1861 3696
Size: 1861 1835
AfeI AGC'GCT
Cuts at: 0 1393 3338 3696
Size: 1393 1945 358
Fragments arranged by size:
1945 1393 358
AflIII A'CryG_T
Cuts at: 0 310 764 2228 3696
Size: 310 454 1464 1468
Fragments arranged by size:
1468 1464 454 310
AgeI A'CCGG_T
Cuts at: 0 2138 3696
Size: 2138 1558
AleI CACnn'nnGTG
Cuts at: 0 311 1930 3696
Size: 311 1619 1766
Fragments arranged by size:
1766 1619 311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2386 3696
Size: 2386 1310
AlwNI CAG_nnn'CTG
Cuts at: 0 823 1149 3501 3561 3689 3696
Size: 823 326 2352 60 128 7
Fragments arranged by size:
2352 823 326 128 60 7
ApaI G_GGCC'C
Cuts at: 0 2072 2083 3461 3542 3696
Size: 2072 11 1378 81 154
Fragments arranged by size:
2072 1378 154 81 11
ApaLI G'TGCA_C
Cuts at: 0 2361 3696
Size: 2361 1335
ApoI r'AATT_y
Cuts at: 0 522 2959 3696
Size: 522 2437 737
Fragments arranged by size:
2437 737 522
AvaI C'yCGr_G
Cuts at: 0 274 1526 2545 2601 3097 3179 3625 3696
Size: 274 1252 1019 56 496 82 446 71
Fragments arranged by size:
1252 1019 496 446 274 82 71 56
BamHI G'GATC_C
Cuts at: 0 3618 3696
Size: 3618 78
BanI G'GyrC_C
Cuts at: 0 19 177 412 1478 1556 1631 1653 2910
Size: 19 158 235 1066 78 75 22 1257
Cuts at: 2910 3175 3352 3481 3696
Size: 265 177 129 215
Fragments arranged by size:
1257 1066 265 235 215 177 158 129
78 75 22 19
BanII G_rGCy'C
Cuts at: 0 281 1533 1835 1924 1998 2021 2072 2083
Size: 281 1252 302 89 74 23 51 11
Cuts at: 2083 2515 2803 3388 3461 3542 3696
Size: 432 288 585 73 81 154
Fragments arranged by size:
1252 585 432 302 288 281 154 89
81 74 73 51 23 11
BbeI G_GCGC'C
Cuts at: 0 23 1482 1560 1657 3356 3696
Size: 23 1459 78 97 1699 340
Fragments arranged by size:
1699 1459 340 97 78 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 783 1104 3696
Size: 178 605 321 2592
Fragments arranged by size:
2592 605 321 178
BbvCI CC'TCA_GC
Cuts at: 0 1636 3073 3290 3696
Size: 1636 1437 217 406
Fragments arranged by size:
1636 1437 406 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 121 155 3696
Size: 121 34 3541
Fragments arranged by size:
3541 121 34
BclI T'GATC_A
Cuts at: 0 825 1421 3696
Size: 825 596 2275
Fragments arranged by size:
2275 825 596
BglI GCCn_nnn'nGGC
Cuts at: 0 2883 3696
Size: 2883 813
BlpI GC'TnA_GC
Cuts at: 0 920 1156 1859 2509 3185 3696
Size: 920 236 703 650 676 511
Fragments arranged by size:
920 703 676 650 511 236
Bme1580I G_kGCm'C
Cuts at: 0 31 415 2072 2083 2365 3461 3520 3542
Size: 31 384 1657 11 282 1096 59 22
Cuts at: 3542 3696
Size: 154
Fragments arranged by size:
1657 1096 384 282 154 59 31 22
11
BmgBI CAC'GTC
Cuts at: 0 32 198 767 3696
Size: 32 166 569 2929
Fragments arranged by size:
2929 569 166 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 978 3027 3696
Size: 978 2049 669
Fragments arranged by size:
2049 978 669
BmtI G_CTAG'C
Cuts at: 0 1252 3696
Size: 1252 2444
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 565 2190 2853 3342 3696
Size: 30 535 1625 663 489 354
Fragments arranged by size:
1625 663 535 489 354 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 589 1306 1423 1715 1930 1966 2221
Size: 58 531 717 117 292 215 36 255
Cuts at: 2221 2653 3696
Size: 432 1043
Fragments arranged by size:
1043 717 531 432 292 255 215 117
58 36
Bpu10I CC'TnA_GC
Cuts at: 0 1351 1546 1636 3073 3290 3327 3696
Size: 1351 195 90 1437 217 37 369
Fragments arranged by size:
1437 1351 369 217 195 90 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1363 2551 3073 3696
Size: 1363 1188 522 623
Fragments arranged by size:
1363 1188 623 522
BsaI GGTCTCn'nnnn_
Cuts at: 0 2054 3094 3622 3696
Size: 2054 1040 528 74
Fragments arranged by size:
2054 1040 528 74
BsaAI yAC'GTr
Cuts at: 0 341 604 2583 3696
Size: 341 263 1979 1113
Fragments arranged by size:
1979 1113 341 263
BsaHI Gr'CG_yC
Cuts at: 0 20 91 1479 1557 1654 1878 2348 3353
Size: 20 71 1388 78 97 224 470 1005
Cuts at: 3353 3572 3696
Size: 219 124
Fragments arranged by size:
1388 1005 470 224 219 124 97 78
71 20
BsaWI w'CCGG_w
Cuts at: 0 94 2138 3696
Size: 94 2044 1558
Fragments arranged by size:
2044 1558 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3006 3036 3696
Size: 3006 30 660
Fragments arranged by size:
3006 660 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 985 1042 1675 1678 1681 1684 1729
Size: 448 537 57 633 3 3 3 45
Cuts at: 1729 1759 1777 1780 1783 1792 1801 1810 1813
Size: 30 18 3 3 9 9 9 3
Cuts at: 1813 1987 2179 2554 2731 2734 2737 2797 2814
Size: 174 192 375 177 3 3 60 17
Cuts at: 2814 3276 3307 3696
Size: 462 31 389
Fragments arranged by size:
633 537 462 448 389 375 192 177
174 60 57 45 31 30 18 17
9 9 9 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 372 753 783 1497 2180 2606 2678 3235
Size: 372 381 30 714 683 426 72 557
Cuts at: 3235 3554 3633 3652 3658 3682 3696
Size: 319 79 19 6 24 14
Fragments arranged by size:
714 683 557 426 381 372 319 79
72 30 24 19 14 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 2278 3696
Size: 694 1584 1418
Fragments arranged by size:
1584 1418 694
BsiEI CG_ry'CG
Cuts at: 0 224 2143 3696
Size: 224 1919 1553
Fragments arranged by size:
1919 1553 224
BsiHKAI G_wGCw'C
Cuts at: 0 281 2279 2365 2546 2552 2747 3696
Size: 281 1998 86 181 6 195 949
Fragments arranged by size:
1998 949 281 195 181 86 6
BsmI GAATG_Cn'
Cuts at: 0 269 3696
Size: 269 3427
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1183 2121 3563 3696
Size: 1183 938 1442 133
Fragments arranged by size:
1442 1183 938 133
Bsp1286I G_dGCh'C
Cuts at: 0 31 281 415 1533 1835 1924 1998 2021
Size: 31 250 134 1118 302 89 74 23
Cuts at: 2021 2072 2083 2279 2365 2515 2546 2552 2747
Size: 51 11 196 86 150 31 6 195
Cuts at: 2747 2803 3388 3461 3520 3542 3696
Size: 56 585 73 59 22 154
Fragments arranged by size:
1118 585 302 250 196 195 154 150
134 89 86 74 73 59 56 51
31 31 23 22 11 6
BspEI T'CCGG_A
Cuts at: 0 94 3696
Size: 94 3602
BspHI T'CATG_A
Cuts at: 0 659 2924 3696
Size: 659 2265 772
Fragments arranged by size:
2265 772 659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 609 1619 3696
Size: 609 1010 2077
Fragments arranged by size:
2077 1010 609
BsrBI CCG'CTC
Cuts at: 0 1689 3696
Size: 1689 2007
BsrFI r'CCGG_y
Cuts at: 0 1280 1475 2138 3270 3436 3696
Size: 1280 195 663 1132 166 260
Fragments arranged by size:
1280 1132 663 260 195 166
BsrGI T'GTAC_A
Cuts at: 0 806 3696
Size: 806 2890
BssSI C'ACGA_G
Cuts at: 0 634 2665 3696
Size: 634 2031 1031
Fragments arranged by size:
2031 1031 634
BstAPI GCAn_nnn'nTGC
Cuts at: 0 525 3696
Size: 525 3171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1932 3696
Size: 1932 1764
BstYI r'GATC_y
Cuts at: 0 2336 3618 3696
Size: 2336 1282 78
Fragments arranged by size:
2336 1282 78
BstZ17I GTA'TAC
Cuts at: 0 780 3696
Size: 780 2916
Bsu36I CC'TnA_GG
Cuts at: 0 1196 3696
Size: 1196 2500
BtgI C'CryG_G
Cuts at: 0 417 965 1488 2432 2871 3347 3530 3696
Size: 417 548 523 944 439 476 183 166
Fragments arranged by size:
944 548 523 476 439 417 183 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2668 3696
Size: 2668 1028
BtsI GCAGTG_nn'
Cuts at: 0 395 2858 2947 3246 3454 3696
Size: 395 2463 89 299 208 242
Fragments arranged by size:
2463 395 299 242 208 89
DraIII CAC_nnn'GTG
Cuts at: 0 558 3526 3696
Size: 558 2968 170
Fragments arranged by size:
2968 558 170
EaeI y'GGCC_r
Cuts at: 0 2 1409 1967 2024 2385 2406 2472 2585
Size: 2 1407 558 57 361 21 66 113
Cuts at: 2585 2612 2624 2775 2868 3220 3503 3533 3696
Size: 27 12 151 93 352 283 30 163
Fragments arranged by size:
1407 558 361 352 283 163 151 113
93 66 57 30 27 21 12 2
EarI CTCTTCn'nnn_
Cuts at: 0 871 1066 1363 1778 2284 2503 3166 3311
Size: 871 195 297 415 506 219 663 145
Cuts at: 3311 3696
Size: 385
Fragments arranged by size:
871 663 506 415 385 297 219 195
145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 1603 2002 2026 2277 3696
Size: 283 1320 399 24 251 1419
Fragments arranged by size:
1419 1320 399 283 251 24
EcoICRI GAG'CTC
Cuts at: 0 279 3696
Size: 279 3417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 589 1306 1423 1715 1861 1930 1966
Size: 58 531 717 117 292 146 69 36
Cuts at: 1966 2221 2653 3696
Size: 255 432 1043
Fragments arranged by size:
1043 717 531 432 292 255 146 117
69 58 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 1909 1969 3289 3696
Size: 1195 714 60 1320 407
Fragments arranged by size:
1320 1195 714 407 60
EcoO109I rG'GnC_Cy
Cuts at: 0 59 1455 1815 1902 2068 2079 2900 3230
Size: 59 1396 360 87 166 11 821 330
Cuts at: 3230 3282 3458 3498 3539 3696
Size: 52 176 40 41 157
Fragments arranged by size:
1396 821 360 330 176 166 157 87
59 52 41 40 11
EcoRV GAT'ATC
Cuts at: 0 2598 3696
Size: 2598 1098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1869 3696
Size: 1869 1827
FspI TGC'GCA
Cuts at: 0 382 3696
Size: 382 3314
HaeII r_GCGC'y
Cuts at: 0 23 1295 1395 1482 1560 1657 1681 2308
Size: 23 1272 100 87 78 97 24 627
Cuts at: 2308 2496 2646 2673 2736 2895 3340 3356 3640
Size: 188 150 27 63 159 445 16 284
Cuts at: 3640 3696
Size: 56
Fragments arranged by size:
1272 627 445 284 188 159 150 100
97 87 78 63 56 27 24 23
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3178 3210 3696
Size: 3178 32 486
Fragments arranged by size:
3178 486 32
HincII GTy'rAC
Cuts at: 0 884 1341 2322 2457 3696
Size: 884 457 981 135 1239
Fragments arranged by size:
1239 981 884 457 135
HindIII A'AGCT_T
Cuts at: 0 109 3696
Size: 109 3587
KasI G'GCGC_C
Cuts at: 0 19 1478 1556 1653 3352 3696
Size: 19 1459 78 97 1699 344
Fragments arranged by size:
1699 1459 344 97 78 19
MluI A'CGCG_T
Cuts at: 0 310 3696
Size: 310 3386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2126 3221 3696
Size: 2126 1095 475
Fragments arranged by size:
2126 1095 475
MscI TGG'CCA
Cuts at: 0 1411 2587 2626 2870 3696
Size: 1411 1176 39 244 826
Fragments arranged by size:
1411 1176 826 244 39
MslI CAynn'nnrTG
Cuts at: 0 262 311 933 1930 2581 3696
Size: 262 49 622 997 651 1115
Fragments arranged by size:
1115 997 651 622 262 49
MspA1I CmG'CkG
Cuts at: 0 419 787 1068 1140 1490 1613 1640 2376
Size: 419 368 281 72 350 123 27 736
Cuts at: 2376 2487 3411 3508 3686 3696
Size: 111 924 97 178 10
Fragments arranged by size:
924 736 419 368 350 281 178 123
111 97 72 27 10
NaeI GCC'GGC
Cuts at: 0 3438 3696
Size: 3438 258
NarI GG'CG_CC
Cuts at: 0 20 1479 1557 1654 3353 3696
Size: 20 1459 78 97 1699 343
Fragments arranged by size:
1699 1459 343 97 78 20
NcoI C'CATG_G
Cuts at: 0 2432 3530 3696
Size: 2432 1098 166
Fragments arranged by size:
2432 1098 166
NgoMIV G'CCGG_C
Cuts at: 0 3436 3696
Size: 3436 260
NheI G'CTAG_C
Cuts at: 0 1248 3696
Size: 1248 2448
PasI CC'CwG_GG
Cuts at: 0 2896 3461 3696
Size: 2896 565 235
Fragments arranged by size:
2896 565 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 2394 3501 3689 3696
Size: 2394 1107 188 7
Fragments arranged by size:
2394 1107 188 7
PfoI T'CCnGG_A
Cuts at: 0 566 587 1820 1907 2234 3696
Size: 566 21 1233 87 327 1462
Fragments arranged by size:
1462 1233 566 327 87 21
PmlI CAC'GTG
Cuts at: 0 604 2583 3696
Size: 604 1979 1113
Fragments arranged by size:
1979 1113 604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2215 2247 3696
Size: 2215 32 1449
Fragments arranged by size:
2215 1449 32
PpuMI rG'GwC_Cy
Cuts at: 0 1455 2900 3230 3696
Size: 1455 1445 330 466
Fragments arranged by size:
1455 1445 466 330
PspOMI G'GGCC_C
Cuts at: 0 2068 2079 3457 3538 3696
Size: 2068 11 1378 81 158
Fragments arranged by size:
2068 1378 158 81 11
PspXI vC'TCGA_Gb
Cuts at: 0 2545 3696
Size: 2545 1151
PstI C_TGCA'G
Cuts at: 0 84 134 521 1145 1463 2375 2492 2954
Size: 84 50 387 624 318 912 117 462
Cuts at: 2954 3000 3696
Size: 46 696
Fragments arranged by size:
912 696 624 462 387 318 117 84
50 46
PvuII CAG'CTG
Cuts at: 0 787 1068 1140 2487 3696
Size: 787 281 72 1347 1209
Fragments arranged by size:
1347 1209 787 281 72
SacI G_AGCT'C
Cuts at: 0 281 3696
Size: 281 3415
SacII CC_GC'GG
Cuts at: 0 420 1491 3696
Size: 420 1071 2205
Fragments arranged by size:
2205 1071 420
SalI G'TCGA_C
Cuts at: 0 882 3696
Size: 882 2814
SanDI GG'GwC_CC
Cuts at: 0 2900 3230 3696
Size: 2900 330 466
Fragments arranged by size:
2900 466 330
SapI GCTCTTCn'nnn_
Cuts at: 0 1066 2284 3166 3696
Size: 1066 1218 882 530
Fragments arranged by size:
1218 1066 882 530
SbfI CC_TGCA'GG
Cuts at: 0 134 3696
Size: 134 3562
SexAI A'CCwGG_T
Cuts at: 0 146 3696
Size: 146 3550
SfcI C'TryA_G
Cuts at: 0 80 130 182 517 1141 1459 2371 2488
Size: 80 50 52 335 624 318 912 117
Cuts at: 2488 2950 2996 3696
Size: 462 46 700
Fragments arranged by size:
912 700 624 462 335 318 117 80
52 50 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2883 3696
Size: 2883 813
SfoI GGC'GCC
Cuts at: 0 21 1480 1558 1655 3354 3696
Size: 21 1459 78 97 1699 342
Fragments arranged by size:
1699 1459 342 97 78 21
SmaI CCC'GGG
Cuts at: 0 2603 3181 3696
Size: 2603 578 515
Fragments arranged by size:
2603 578 515
SmlI C'TyrA_G
Cuts at: 0 1378 2545 2566 3088 3625 3696
Size: 1378 1167 21 522 537 71
Fragments arranged by size:
1378 1167 537 522 71 21
StuI AGG'CCT
Cuts at: 0 2653 2836 3696
Size: 2653 183 860
Fragments arranged by size:
2653 860 183
StyI C'CwwG_G
Cuts at: 0 2432 2843 2849 3530 3696
Size: 2432 411 6 681 166
Fragments arranged by size:
2432 681 411 166 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 660 2203 2425 2598 2928 3054 3696
Size: 238 422 1543 222 173 330 126 642
Fragments arranged by size:
1543 642 422 330 238 222 173 126
TatI w'GTAC_w
Cuts at: 0 740 806 3696
Size: 740 66 2890
Fragments arranged by size:
2890 740 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3006 3038 3603 3635 3696
Size: 3006 32 565 32 61
Fragments arranged by size:
3006 565 61 32 32
XbaI T'CTAG_A
Cuts at: 0 699 3696
Size: 699 2997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2532 2985 3696
Size: 2532 453 711
Fragments arranged by size:
2532 711 453
XhoI C'TCGA_G
Cuts at: 0 2545 3625 3696
Size: 2545 1080 71
Fragments arranged by size:
2545 1080 71
XmaI C'CCGG_G
Cuts at: 0 2601 3179 3696
Size: 2601 578 517
Fragments arranged by size:
2601 578 517
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AloI AscI AseI
AsiSI AvrII BaeI BciVI BfrBI BglII BsaBI BsiWI
BsrDI BssHII BstBI BstEII ClaI CspCI DraI DrdI
EagI EcoRI FseI FspAI HpaI KpnI MfeI NdeI
NotI NruI NsiI NspI PacI PciI PmeI PshAI
PsiI PsrI PvuI RsrII ScaI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XmnI ZraI