Restriction map for
pF1KA1621
(Linear) (Six-base) MAPSORT of: KIAA1621.seq Check: 8624 from: 1 to: 2328
KIAA1621.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 996 1499 2328
Size: 996 503 829
Fragments arranged by size:
996 829 503
AccI GT'mk_AC
Cuts at: 0 2063 2328
Size: 2063 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 2320 2328
Size: 1169 1151 8
Fragments arranged by size:
1169 1151 8
AfeI AGC'GCT
Cuts at: 0 641 2328
Size: 641 1687
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 2328
Size: 1567 32 729
Fragments arranged by size:
1567 729 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 2328
Size: 1117 1211
ApaI G_GGCC'C
Cuts at: 0 2191 2328
Size: 2191 137
ApaLI G'TGCA_C
Cuts at: 0 990 2328
Size: 990 1338
ApoI r'AATT_y
Cuts at: 0 550 2328
Size: 550 1778
AvaI C'yCGr_G
Cuts at: 0 1730 1785 1834 2328
Size: 1730 55 49 494
Fragments arranged by size:
1730 494 55 49
BanI G'GyrC_C
Cuts at: 0 79 1725 2221 2328
Size: 79 1646 496 107
Fragments arranged by size:
1646 496 107 79
BanII G_rGCy'C
Cuts at: 0 1835 2191 2328
Size: 1835 356 137
Fragments arranged by size:
1835 356 137
BbeI G_GCGC'C
Cuts at: 0 83 2328
Size: 83 2245
BbsI GAAGACnn'nnnn_
Cuts at: 0 1144 1166 2328
Size: 1144 22 1162
Fragments arranged by size:
1162 1144 22
BlpI GC'TnA_GC
Cuts at: 0 61 1888 2328
Size: 61 1827 440
Fragments arranged by size:
1827 440 61
Bme1580I G_kGCm'C
Cuts at: 0 994 1730 2191 2328
Size: 994 736 461 137
Fragments arranged by size:
994 736 461 137
BmgBI CAC'GTC
Cuts at: 0 1017 2328
Size: 1017 1311
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 2328
Size: 251 2077
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 2328
Size: 141 2187
Bpu10I CC'TnA_GC
Cuts at: 0 575 677 2328
Size: 575 102 1651
Fragments arranged by size:
1651 575 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1804 2328
Size: 214 1590 524
Fragments arranged by size:
1590 524 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 1515 2328
Size: 1515 813
BsaAI yAC'GTr
Cuts at: 0 1977 2328
Size: 1977 351
BsaHI Gr'CG_yC
Cuts at: 0 80 1658 2202 2328
Size: 80 1578 544 126
Fragments arranged by size:
1578 544 126 80
BsaWI w'CCGG_w
Cuts at: 0 426 1123 2328
Size: 426 697 1205
Fragments arranged by size:
1205 697 426
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1567 1594 1597 1624 1981 2011 2328
Size: 1567 27 3 27 357 30 317
Fragments arranged by size:
1567 357 317 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 622 625 2160 2328
Size: 292 330 3 1535 168
Fragments arranged by size:
1535 330 292 168 3
BseYI C'CCAG_C
Cuts at: 0 2000 2328
Size: 2000 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1034 1336 1387 1698 1957 2160 2328
Size: 766 268 302 51 311 259 203 168
Fragments arranged by size:
766 311 302 268 259 203 168 51
BsiEI CG_ry'CG
Cuts at: 0 1739 2328
Size: 1739 589
BsiHKAI G_wGCw'C
Cuts at: 0 994 1224 1304 1957 1982 2117 2328
Size: 994 230 80 653 25 135 211
Fragments arranged by size:
994 653 230 211 135 80 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 795 2328
Size: 795 1533
Bsp1286I G_dGCh'C
Cuts at: 0 994 1224 1304 1730 1835 1957 1982 2117
Size: 994 230 80 426 105 122 25 135
Cuts at: 2117 2191 2328
Size: 74 137
Fragments arranged by size:
994 426 230 137 135 122 105 80
74 25
BspEI T'CCGG_A
Cuts at: 0 1123 2328
Size: 1123 1205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 996 1499 2328
Size: 996 503 829
Fragments arranged by size:
996 829 503
BsrBI CCG'CTC
Cuts at: 0 2016 2328
Size: 2016 312
BstBI TT'CG_AA
Cuts at: 0 926 2328
Size: 926 1402
BstEII G'GTnAC_C
Cuts at: 0 1038 1455 1755 2328
Size: 1038 417 300 573
Fragments arranged by size:
1038 573 417 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1767 2328
Size: 1767 561
BstYI r'GATC_y
Cuts at: 0 1113 2328
Size: 1113 1215
Bsu36I CC'TnA_GG
Cuts at: 0 1546 2183 2328
Size: 1546 637 145
Fragments arranged by size:
1546 637 145
BtgI C'CryG_G
Cuts at: 0 1605 1826 2328
Size: 1605 221 502
Fragments arranged by size:
1605 502 221
DrdI GACnn_nn'nnGTC
Cuts at: 0 174 2328
Size: 174 2154
EaeI y'GGCC_r
Cuts at: 0 1530 1736 1958 2034 2328
Size: 1530 206 222 76 294
Fragments arranged by size:
1530 294 222 206 76
EagI C'GGCC_G
Cuts at: 0 1736 2328
Size: 1736 592
EarI CTCTTCn'nnn_
Cuts at: 0 1001 2104 2328
Size: 1001 1103 224
Fragments arranged by size:
1103 1001 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 783 827 2328
Size: 783 44 1501
Fragments arranged by size:
1501 783 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 2320 2328
Size: 1169 1151 8
Fragments arranged by size:
1169 1151 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 890 2328
Size: 890 1438
EcoO109I rG'GnC_Cy
Cuts at: 0 92 1482 1566 2187 2188 2228 2328
Size: 92 1390 84 621 1 40 100
Fragments arranged by size:
1390 621 100 92 84 40 1
EcoRI G'AATT_C
Cuts at: 0 550 2328
Size: 550 1778
EcoRV GAT'ATC
Cuts at: 0 1322 2328
Size: 1322 1006
FspI TGC'GCA
Cuts at: 0 952 1873 2328
Size: 952 921 455
Fragments arranged by size:
952 921 455
FspAI rTGC'GCAy
Cuts at: 0 952 1873 2328
Size: 952 921 455
Fragments arranged by size:
952 921 455
HaeII r_GCGC'y
Cuts at: 0 83 643 1424 1598 1636 2328
Size: 83 560 781 174 38 692
Fragments arranged by size:
781 692 560 174 83 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 76 108 637 669 1105 1137
Size: 6 32 38 32 529 32 436 32
Cuts at: 1137 1420 1452 1594 1626 1768 1800 1981 2013
Size: 283 32 142 32 142 32 181 32
Cuts at: 2013 2328
Size: 315
Fragments arranged by size:
529 436 315 283 181 142 142 38
32 32 32 32 32 32 32 32
6
HincII GTy'rAC
Cuts at: 0 167 2328
Size: 167 2161
KasI G'GCGC_C
Cuts at: 0 79 2328
Size: 79 2249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 2328
Size: 566 1762
MslI CAynn'nnrTG
Cuts at: 0 1273 1312 2328
Size: 1273 39 1016
Fragments arranged by size:
1273 1016 39
MspA1I CmG'CkG
Cuts at: 0 1607 1692 1815 2171 2328
Size: 1607 85 123 356 157
Fragments arranged by size:
1607 356 157 123 85
NarI GG'CG_CC
Cuts at: 0 80 2328
Size: 80 2248
PasI CC'CwG_GG
Cuts at: 0 204 2328
Size: 204 2124
PfoI T'CCnGG_A
Cuts at: 0 1154 2328
Size: 1154 1174
PmlI CAC'GTG
Cuts at: 0 1977 2328
Size: 1977 351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 2278 2310 2328
Size: 1567 32 679 32 18
Fragments arranged by size:
1567 679 32 32 18
PpuMI rG'GwC_Cy
Cuts at: 0 92 1482 2228 2328
Size: 92 1390 746 100
Fragments arranged by size:
1390 746 100 92
PspOMI G'GGCC_C
Cuts at: 0 2187 2328
Size: 2187 141
PstI C_TGCA'G
Cuts at: 0 1007 1697 2328
Size: 1007 690 631
Fragments arranged by size:
1007 690 631
PvuII CAG'CTG
Cuts at: 0 1815 2328
Size: 1815 513
SacII CC_GC'GG
Cuts at: 0 1608 2328
Size: 1608 720
SanDI GG'GwC_CC
Cuts at: 0 2228 2328
Size: 2228 100
SapI GCTCTTCn'nnn_
Cuts at: 0 2104 2328
Size: 2104 224
SbfI CC_TGCA'GG
Cuts at: 0 1007 2328
Size: 1007 1321
SexAI A'CCwGG_T
Cuts at: 0 1751 2066 2328
Size: 1751 315 262
Fragments arranged by size:
1751 315 262
SfcI C'TryA_G
Cuts at: 0 1003 1693 2328
Size: 1003 690 635
Fragments arranged by size:
1003 690 635
SfoI GGC'GCC
Cuts at: 0 81 2328
Size: 81 2247
SmaI CCC'GGG
Cuts at: 0 1732 1836 2328
Size: 1732 104 492
Fragments arranged by size:
1732 492 104
SmlI C'TyrA_G
Cuts at: 0 229 1819 2328
Size: 229 1590 509
Fragments arranged by size:
1590 509 229
SrfI GCCC'GGGC
Cuts at: 0 1836 2328
Size: 1836 492
SspI AAT'ATT
Cuts at: 0 347 2328
Size: 347 1981
StyI C'CwwG_G
Cuts at: 0 1205 1285 1760 2328
Size: 1205 80 475 568
Fragments arranged by size:
1205 568 475 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1369 2328
Size: 1369 959
Tth111I GACn'n_nGTC
Cuts at: 0 1044 2232 2328
Size: 1044 1188 96
Fragments arranged by size:
1188 1044 96
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1914 2328
Size: 1914 414
XmaI C'CCGG_G
Cuts at: 0 1730 1834 2328
Size: 1730 104 494
Fragments arranged by size:
1730 494 104
XmnI GAAnn'nnTTC
Cuts at: 0 347 413 1163 2328
Size: 347 66 750 1165
Fragments arranged by size:
1165 750 347 66
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BstBI BstEII BstXI BstYI
Bsu36I BtgI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I
HincII KasI MmeI MslI MspA1I NarI PasI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI
SapI SbfI SexAI SfcI SfoI SmaI SmlI SrfI
SspI StyI TaqII Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmtI BpmI
BsaBI BsiWI BsmI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII EcoICRI FalI FseI HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI StuI SwaI
TatI TstI XbaI XhoI ZraI