Restriction map for
pF1KSDA1612
(Linear) (Six-base) MAPSORT of: KIAA1612.seq Check: 390 from: 1 to: 1626
KIAA1612.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 102 1626
Size: 102 1524
AccI GT'mk_AC
Cuts at: 0 1508 1626
Size: 1508 118
Acc65I G'GTAC_C
Cuts at: 0 538 1626
Size: 538 1088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 683 695 904 1108 1184 1626
Size: 220 463 12 209 204 76 442
Fragments arranged by size:
463 442 220 209 204 76 12
AflII C'TTAA_G
Cuts at: 0 895 1626
Size: 895 731
AflIII A'CryG_T
Cuts at: 0 419 942 1626
Size: 419 523 684
Fragments arranged by size:
684 523 419
AgeI A'CCGG_T
Cuts at: 0 1330 1626
Size: 1330 296
ApoI r'AATT_y
Cuts at: 0 350 439 845 875 907 1369 1531 1626
Size: 350 89 406 30 32 462 162 95
Fragments arranged by size:
462 406 350 162 95 89 32 30
AvaI C'yCGr_G
Cuts at: 0 35 1626
Size: 35 1591
BanI G'GyrC_C
Cuts at: 0 482 538 1626
Size: 482 56 1088
Fragments arranged by size:
1088 482 56
BanII G_rGCy'C
Cuts at: 0 26 769 1626
Size: 26 743 857
Fragments arranged by size:
857 743 26
BbeI G_GCGC'C
Cuts at: 0 486 1626
Size: 486 1140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1333 1626
Size: 1333 293
BbvCI CC'TCA_GC
Cuts at: 0 119 995 1626
Size: 119 876 631
Fragments arranged by size:
876 631 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1626
Size: 147 1479
Bpu10I CC'TnA_GC
Cuts at: 0 119 995 1190 1626
Size: 119 876 195 436
Fragments arranged by size:
876 436 195 119
BsaBI GATnn'nnATC
Cuts at: 0 1213 1626
Size: 1213 413
BsaHI Gr'CG_yC
Cuts at: 0 139 483 1626
Size: 139 344 1143
Fragments arranged by size:
1143 344 139
BsaWI w'CCGG_w
Cuts at: 0 368 1330 1626
Size: 368 962 296
Fragments arranged by size:
962 368 296
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 519 549 717 747 1626
Size: 519 30 168 30 879
Fragments arranged by size:
879 519 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1626
Size: 1012 614
BseYI C'CCAG_C
Cuts at: 0 30 1409 1626
Size: 30 1379 217
Fragments arranged by size:
1379 217 30
Bsp1286I G_dGCh'C
Cuts at: 0 26 769 1626
Size: 26 743 857
Fragments arranged by size:
857 743 26
BspHI T'CATG_A
Cuts at: 0 786 1350 1626
Size: 786 564 276
Fragments arranged by size:
786 564 276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 102 1626
Size: 102 1524
BsrDI GCAATG_nn'
Cuts at: 0 1245 1442 1626
Size: 1245 197 184
Fragments arranged by size:
1245 197 184
BsrFI r'CCGG_y
Cuts at: 0 1330 1626
Size: 1330 296
BsrGI T'GTAC_A
Cuts at: 0 551 1626
Size: 551 1075
DraIII CAC_nnn'GTG
Cuts at: 0 192 657 1626
Size: 192 465 969
Fragments arranged by size:
969 465 192
EaeI y'GGCC_r
Cuts at: 0 13 1211 1626
Size: 13 1198 415
Fragments arranged by size:
1198 415 13
EarI CTCTTCn'nnn_
Cuts at: 0 310 315 848 1457 1626
Size: 310 5 533 609 169
Fragments arranged by size:
609 533 310 169 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 1626
Size: 85 1541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 220 683 695 904 1108 1184 1626
Size: 147 73 463 12 209 204 76 442
Fragments arranged by size:
463 442 209 204 147 76 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 522 1626
Size: 522 1104
EcoO109I rG'GnC_Cy
Cuts at: 0 964 1626
Size: 964 662
EcoRI G'AATT_C
Cuts at: 0 439 1626
Size: 439 1187
EcoRV GAT'ATC
Cuts at: 0 1158 1626
Size: 1158 468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1511 1593 1626
Size: 1511 82 33
Fragments arranged by size:
1511 82 33
HaeII r_GCGC'y
Cuts at: 0 486 1626
Size: 486 1140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 391 423 717 749 1626
Size: 355 32 4 32 294 32 877
Fragments arranged by size:
877 355 294 32 32 32 4
HindIII A'AGCT_T
Cuts at: 0 1006 1090 1626
Size: 1006 84 536
Fragments arranged by size:
1006 536 84
KasI G'GCGC_C
Cuts at: 0 482 1626
Size: 482 1144
KpnI G_GTAC'C
Cuts at: 0 542 1626
Size: 542 1084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1626
Size: 1097 529
MscI TGG'CCA
Cuts at: 0 1213 1626
Size: 1213 413
MslI CAynn'nnrTG
Cuts at: 0 163 169 418 467 941 1626
Size: 163 6 249 49 474 685
Fragments arranged by size:
685 474 249 163 49 6
MspA1I CmG'CkG
Cuts at: 0 19 1626
Size: 19 1607
NarI GG'CG_CC
Cuts at: 0 483 1626
Size: 483 1143
NspI r_CATG'y
Cuts at: 0 423 946 1626
Size: 423 523 680
Fragments arranged by size:
680 523 423
PasI CC'CwG_GG
Cuts at: 0 518 1626
Size: 518 1108
PciI A'CATG_T
Cuts at: 0 419 942 1626
Size: 419 523 684
Fragments arranged by size:
684 523 419
PflMI CCAn_nnn'nTGG
Cuts at: 0 1423 1626
Size: 1423 203
PpuMI rG'GwC_Cy
Cuts at: 0 964 1626
Size: 964 662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 740 772 1626
Size: 740 32 854
Fragments arranged by size:
854 740 32
SapI GCTCTTCn'nnn_
Cuts at: 0 1457 1626
Size: 1457 169
SexAI A'CCwGG_T
Cuts at: 0 506 653 1626
Size: 506 147 973
Fragments arranged by size:
973 506 147
SfcI C'TryA_G
Cuts at: 0 1509 1626
Size: 1509 117
SfoI GGC'GCC
Cuts at: 0 484 1626
Size: 484 1142
SmaI CCC'GGG
Cuts at: 0 37 1626
Size: 37 1589
SmlI C'TyrA_G
Cuts at: 0 895 1626
Size: 895 731
StuI AGG'CCT
Cuts at: 0 124 928 1085 1626
Size: 124 804 157 541
Fragments arranged by size:
804 541 157 124
StyI C'CwwG_G
Cuts at: 0 929 1305 1626
Size: 929 376 321
Fragments arranged by size:
929 376 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 523 1626
Size: 523 1103
TatI w'GTAC_w
Cuts at: 0 551 1391 1626
Size: 551 840 235
Fragments arranged by size:
840 551 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 1626
Size: 519 32 1075
Fragments arranged by size:
1075 519 32
XmaI C'CCGG_G
Cuts at: 0 35 1626
Size: 35 1591
XmnI GAAnn'nnTTC
Cuts at: 0 233 569 1576 1626
Size: 233 336 1007 50
Fragments arranged by size:
1007 336 233 50
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AgeI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BpmI Bpu10I
BsaBI BsaHI BsaWI BsaXI BseRI BseYI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NspI PasI PciI PflMI PpuMI PsrI SapI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AhdI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI
EcoICRI FseI FspI FspAI HincII HpaI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
ZraI