Restriction map for
pF1KA1604
(Linear) (Six-base) MAPSORT of: KIAA1604.seq Check: 7152 from: 1 to: 2724
KIAA1604.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 2000 2015 2724
Size: 505 1495 15 709
Fragments arranged by size:
1495 709 505 15
AflII C'TTAA_G
Cuts at: 0 889 2724
Size: 889 1835
AlwNI CAG_nnn'CTG
Cuts at: 0 1767 2038 2724
Size: 1767 271 686
Fragments arranged by size:
1767 686 271
ApoI r'AATT_y
Cuts at: 0 673 772 1211 1513 1739 2310 2481 2532
Size: 673 99 439 302 226 571 171 51
Cuts at: 2532 2724
Size: 192
Fragments arranged by size:
673 571 439 302 226 192 171 99
51
AseI AT'TA_AT
Cuts at: 0 497 694 2724
Size: 497 197 2030
Fragments arranged by size:
2030 497 197
AvaI C'yCGr_G
Cuts at: 0 119 1850 2724
Size: 119 1731 874
Fragments arranged by size:
1731 874 119
BamHI G'GATC_C
Cuts at: 0 366 1161 2724
Size: 366 795 1563
Fragments arranged by size:
1563 795 366
BanI G'GyrC_C
Cuts at: 0 2255 2620 2724
Size: 2255 365 104
Fragments arranged by size:
2255 365 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 1777 2078 2176 2247 2724
Size: 95 1682 301 98 71 477
Fragments arranged by size:
1682 477 301 98 95 71
BbvCI CC'TCA_GC
Cuts at: 0 2136 2724
Size: 2136 588
BclI T'GATC_A
Cuts at: 0 1089 2198 2724
Size: 1089 1109 526
Fragments arranged by size:
1109 1089 526
BfrBI ATG'CAT
Cuts at: 0 2424 2724
Size: 2424 300
BlpI GC'TnA_GC
Cuts at: 0 453 2724
Size: 453 2271
Bme1580I G_kGCm'C
Cuts at: 0 1494 2258 2724
Size: 1494 764 466
Fragments arranged by size:
1494 764 466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 638 2058 2121 2724
Size: 638 1420 63 603
Fragments arranged by size:
1420 638 603 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1135 2693 2724
Size: 68 1067 1558 31
Fragments arranged by size:
1558 1067 68 31
Bpu10I CC'TnA_GC
Cuts at: 0 326 415 2136 2724
Size: 326 89 1721 588
Fragments arranged by size:
1721 588 326 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2574 2724
Size: 2574 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 1362 2405 2724
Size: 266 1096 1043 319
Fragments arranged by size:
1096 1043 319 266
BsaBI GATnn'nnATC
Cuts at: 0 248 2609 2724
Size: 248 2361 115
Fragments arranged by size:
2361 248 115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 300 2016 2483 2724
Size: 69 231 1716 467 241
Fragments arranged by size:
1716 467 241 231 69
BseYI C'CCAG_C
Cuts at: 0 1255 1531 2724
Size: 1255 276 1193
Fragments arranged by size:
1255 1193 276
BsiHKAI G_wGCw'C
Cuts at: 0 1419 2724
Size: 1419 1305
BsmI GAATG_Cn'
Cuts at: 0 162 2724
Size: 162 2562
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2622 2724
Size: 2622 102
Bsp1286I G_dGCh'C
Cuts at: 0 1419 1494 2258 2724
Size: 1419 75 764 466
Fragments arranged by size:
1419 764 466 75
BspHI T'CATG_A
Cuts at: 0 39 1332 2724
Size: 39 1293 1392
Fragments arranged by size:
1392 1293 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 2724
Size: 345 2379
BsrFI r'CCGG_y
Cuts at: 0 2690 2724
Size: 2690 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 616 2724
Size: 616 2108
BstBI TT'CG_AA
Cuts at: 0 723 957 2724
Size: 723 234 1767
Fragments arranged by size:
1767 723 234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1255 2724
Size: 1255 1469
BstYI r'GATC_y
Cuts at: 0 366 1161 2334 2724
Size: 366 795 1173 390
Fragments arranged by size:
1173 795 390 366
BtgI C'CryG_G
Cuts at: 0 1681 2724
Size: 1681 1043
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1594 2724
Size: 1594 1130
BtsI GCAGTG_nn'
Cuts at: 0 2073 2724
Size: 2073 651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 758 793 2724
Size: 758 35 1931
Fragments arranged by size:
1931 758 35
DraIII CAC_nnn'GTG
Cuts at: 0 616 2724
Size: 616 2108
EarI CTCTTCn'nnn_
Cuts at: 0 165 377 573 1178 1262 1280 1286 2013
Size: 165 212 196 605 84 18 6 727
Cuts at: 2013 2019 2034 2090 2112 2458 2691 2724
Size: 6 15 56 22 346 233 33
Fragments arranged by size:
727 605 346 233 212 196 165 84
56 33 22 18 15 6 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 505 1135 2000 2015 2693 2724
Size: 68 437 630 865 15 678 31
Fragments arranged by size:
865 678 630 437 68 31 15
EcoO109I rG'GnC_Cy
Cuts at: 0 480 2724
Size: 480 2244
EcoRV GAT'ATC
Cuts at: 0 2611 2724
Size: 2611 113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 571 1159 1231 2501 2724
Size: 571 588 72 1270 223
Fragments arranged by size:
1270 588 571 223 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 1219 1251 1638 1670 2095 2127
Size: 680 32 507 32 387 32 425 32
Cuts at: 2127 2724
Size: 597
Fragments arranged by size:
680 597 507 425 387 32 32 32
32
HincII GTy'rAC
Cuts at: 0 519 2724
Size: 519 2205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 2072 2724
Size: 551 1521 652
Fragments arranged by size:
1521 652 551
MslI CAynn'nnrTG
Cuts at: 0 641 1102 1680 2724
Size: 641 461 578 1044
Fragments arranged by size:
1044 641 578 461
NcoI C'CATG_G
Cuts at: 0 1681 2724
Size: 1681 1043
NdeI CA'TA_TG
Cuts at: 0 1103 2724
Size: 1103 1621
NsiI A_TGCA'T
Cuts at: 0 2426 2724
Size: 2426 298
NspI r_CATG'y
Cuts at: 0 1548 2724
Size: 1548 1176
PflMI CCAn_nnn'nTGG
Cuts at: 0 280 685 801 1614 2724
Size: 280 405 116 813 1110
Fragments arranged by size:
1110 813 405 280 116
PfoI T'CCnGG_A
Cuts at: 0 842 1760 2724
Size: 842 918 964
Fragments arranged by size:
964 918 842
PshAI GACnn'nnGTC
Cuts at: 0 588 2724
Size: 588 2136
PstI C_TGCA'G
Cuts at: 0 1826 2724
Size: 1826 898
SexAI A'CCwGG_T
Cuts at: 0 1352 2724
Size: 1352 1372
SfcI C'TryA_G
Cuts at: 0 1822 1898 2388 2724
Size: 1822 76 490 336
Fragments arranged by size:
1822 490 336 76
SmaI CCC'GGG
Cuts at: 0 121 2724
Size: 121 2603
SmlI C'TyrA_G
Cuts at: 0 889 2589 2724
Size: 889 1700 135
Fragments arranged by size:
1700 889 135
SphI G_CATG'C
Cuts at: 0 1548 2724
Size: 1548 1176
SspI AAT'ATT
Cuts at: 0 1754 2724
Size: 1754 970
StyI C'CwwG_G
Cuts at: 0 1681 2724
Size: 1681 1043
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 230 649 2724
Size: 230 419 2075
Fragments arranged by size:
2075 419 230
TatI w'GTAC_w
Cuts at: 0 1190 1559 2348 2724
Size: 1190 369 789 376
Fragments arranged by size:
1190 789 376 369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1611 2724
Size: 1611 1113
XmaI C'CCGG_G
Cuts at: 0 119 2724
Size: 119 2605
XmnI GAAnn'nnTTC
Cuts at: 0 953 1139 2481 2724
Size: 953 186 1342 243
Fragments arranged by size:
1342 953 243 186
Enzymes that do cut:
AcuI AflII AlwNI ApoI AseI AvaI BamHI BanI
BbsI BbvCI BclI BfrBI BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrFI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII EarI Eco57MI
EcoO109I EcoRV FalI Hin4I HincII MmeI MslI NcoI
NdeI NsiI NspI PflMI PfoI PshAI PstI SexAI
SfcI SmaI SmlI SphI SspI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BanII BbeI BcgI BciVI BglI BglII
BmgBI BmrI BmtI BsaAI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstEII BstZ17I Bsu36I ClaI DraI DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XhoI
ZraI