(Linear) (Six-base) MAPSORT of: KIAA1592.seq Check: 5653 from: 1 to: 1893 KIAA1592.orf With 182 enzymes: SgfI * October 24, 2006 15:08 .. AatII G_ACGT'C Cuts at: 0 1586 1893 Size: 1586 307 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 1644 1893 Size: 70 1574 249 Fragments arranged by size: 1574 249 70 AflIII A'CryG_T Cuts at: 0 1106 1893 Size: 1106 787 AhdI GACnn_n'nnGTC Cuts at: 0 810 1893 Size: 810 1083 AlwNI CAG_nnn'CTG Cuts at: 0 435 1893 Size: 435 1458 ApaI G_GGCC'C Cuts at: 0 30 44 1893 Size: 30 14 1849 Fragments arranged by size: 1849 30 14 ApaLI G'TGCA_C Cuts at: 0 18 913 1773 1893 Size: 18 895 860 120 Fragments arranged by size: 895 860 120 18 ApoI r'AATT_y Cuts at: 0 487 1893 Size: 487 1406 AvaI C'yCGr_G Cuts at: 0 23 178 1893 Size: 23 155 1715 Fragments arranged by size: 1715 155 23 AvrII C'CTAG_G Cuts at: 0 309 1893 Size: 309 1584 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1291 1324 1893 Size: 1291 33 569 Fragments arranged by size: 1291 569 33 BanI G'GyrC_C Cuts at: 0 229 463 658 1468 1893 Size: 229 234 195 810 425 Fragments arranged by size: 810 425 234 229 195 BanII G_rGCy'C Cuts at: 0 30 44 269 617 644 1193 1893 Size: 30 14 225 348 27 549 700 Fragments arranged by size: 700 549 348 225 30 27 14 BbeI G_GCGC'C Cuts at: 0 1472 1893 Size: 1472 421 BbsI GAAGACnn'nnnn_ Cuts at: 0 809 915 1893 Size: 809 106 978 Fragments arranged by size: 978 809 106 BglI GCCn_nnn'nGGC Cuts at: 0 39 114 273 1893 Size: 39 75 159 1620 Fragments arranged by size: 1620 159 75 39 BlpI GC'TnA_GC Cuts at: 0 1551 1893 Size: 1551 342 Bme1580I G_kGCm'C Cuts at: 0 22 30 44 663 917 1674 1777 1893 Size: 22 8 14 619 254 757 103 116 Fragments arranged by size: 757 619 254 116 103 22 14 8 BmgBI CAC'GTC Cuts at: 0 1062 1893 Size: 1062 831 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1872 1893 Size: 1872 21 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 973 1075 1893 Size: 691 282 102 818 Fragments arranged by size: 818 691 282 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 1288 1315 1893 Size: 865 423 27 578 Fragments arranged by size: 865 578 423 27 BsaI GGTCTCn'nnnn_ Cuts at: 0 1257 1822 1893 Size: 1257 565 71 Fragments arranged by size: 1257 565 71 BsaHI Gr'CG_yC Cuts at: 0 1469 1583 1893 Size: 1469 114 310 Fragments arranged by size: 1469 310 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1858 1888 1893 Size: 1858 30 5 Fragments arranged by size: 1858 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 378 652 970 1084 1207 1893 Size: 103 275 274 318 114 123 686 Fragments arranged by size: 686 318 275 274 123 114 103 BseYI C'CCAG_C Cuts at: 0 29 701 1524 1893 Size: 29 672 823 369 Fragments arranged by size: 823 672 369 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 238 855 1012 1849 1893 Size: 103 135 617 157 837 44 Fragments arranged by size: 837 617 157 135 103 44 BsiEI CG_ry'CG Cuts at: 0 1394 1518 1893 Size: 1394 124 375 Fragments arranged by size: 1394 375 124 BsiHKAI G_wGCw'C Cuts at: 0 22 442 644 917 1193 1346 1777 1893 Size: 22 420 202 273 276 153 431 116 Fragments arranged by size: 431 420 276 273 202 153 116 22 BsmI GAATG_Cn' Cuts at: 0 1890 1893 Size: 1890 3 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1456 1590 1893 Size: 1456 134 303 Fragments arranged by size: 1456 303 134 Bsp1286I G_dGCh'C Cuts at: 0 22 30 44 269 442 617 644 663 Size: 22 8 14 225 173 175 27 19 Cuts at: 663 917 1193 1346 1674 1777 1893 Size: 254 276 153 328 103 116 Fragments arranged by size: 328 276 254 225 175 173 153 116 103 27 22 19 14 8 BspHI T'CATG_A Cuts at: 0 695 719 1893 Size: 695 24 1174 Fragments arranged by size: 1174 695 24 BsrBI CCG'CTC Cuts at: 0 1228 1550 1893 Size: 1228 322 343 Fragments arranged by size: 1228 343 322 BsrFI r'CCGG_y Cuts at: 0 1389 1893 Size: 1389 504 BsrGI T'GTAC_A Cuts at: 0 1109 1373 1893 Size: 1109 264 520 Fragments arranged by size: 1109 520 264 BssSI C'ACGA_G Cuts at: 0 628 1675 1879 1893 Size: 628 1047 204 14 Fragments arranged by size: 1047 628 204 14 BstBI TT'CG_AA Cuts at: 0 800 1893 Size: 800 1093 BstEII G'GTnAC_C Cuts at: 0 1018 1047 1893 Size: 1018 29 846 Fragments arranged by size: 1018 846 29 BstXI CCAn_nnnn'nTGG Cuts at: 0 1785 1893 Size: 1785 108 BstYI r'GATC_y Cuts at: 0 419 1088 1286 1893 Size: 419 669 198 607 Fragments arranged by size: 669 607 419 198 Bsu36I CC'TnA_GG Cuts at: 0 428 1893 Size: 428 1465 BtgI C'CryG_G Cuts at: 0 200 1893 Size: 200 1693 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 746 1216 1893 Size: 746 470 677 Fragments arranged by size: 746 677 470 BtsI GCAGTG_nn' Cuts at: 0 70 1893 Size: 70 1823 DraIII CAC_nnn'GTG Cuts at: 0 912 1893 Size: 912 981 DrdI GACnn_nn'nnGTC Cuts at: 0 1663 1893 Size: 1663 230 EaeI y'GGCC_r Cuts at: 0 983 1223 1391 1796 1893 Size: 983 240 168 405 97 Fragments arranged by size: 983 405 240 168 97 EagI C'GGCC_G Cuts at: 0 1391 1893 Size: 1391 502 EarI CTCTTCn'nnn_ Cuts at: 0 82 1844 1893 Size: 82 1762 49 Fragments arranged by size: 1762 82 49 EcoICRI GAG'CTC Cuts at: 0 642 1191 1893 Size: 642 549 702 Fragments arranged by size: 702 642 549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 691 973 1075 1644 1893 Size: 70 621 282 102 569 249 Fragments arranged by size: 621 569 282 249 102 70 EcoNI CCTnn'n_nnAGG Cuts at: 0 427 1893 Size: 427 1466 EcoO109I rG'GnC_Cy Cuts at: 0 41 432 1035 1893 Size: 41 391 603 858 Fragments arranged by size: 858 603 391 41 EcoRV GAT'ATC Cuts at: 0 693 1277 1893 Size: 693 584 616 Fragments arranged by size: 693 616 584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1176 1893 Size: 1176 717 FspI TGC'GCA Cuts at: 0 211 1893 Size: 211 1682 HaeII r_GCGC'y Cuts at: 0 249 1472 1893 Size: 249 1223 421 Fragments arranged by size: 1223 421 249 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 52 84 411 443 796 828 876 908 Size: 52 32 327 32 353 32 48 32 Cuts at: 908 1821 1853 1893 Size: 913 32 40 Fragments arranged by size: 913 353 327 52 48 40 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 327 1893 Size: 327 1566 KasI G'GCGC_C Cuts at: 0 1468 1893 Size: 1468 425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 740 920 1064 1127 1538 1893 Size: 740 180 144 63 411 355 Fragments arranged by size: 740 411 355 180 144 63 MscI TGG'CCA Cuts at: 0 985 1893 Size: 985 908 MslI CAynn'nnrTG Cuts at: 0 379 793 1783 1883 1893 Size: 379 414 990 100 10 Fragments arranged by size: 990 414 379 100 10 NaeI GCC'GGC Cuts at: 0 1391 1893 Size: 1391 502 NarI GG'CG_CC Cuts at: 0 1469 1893 Size: 1469 424 NcoI C'CATG_G Cuts at: 0 200 1893 Size: 200 1693 NgoMIV G'CCGG_C Cuts at: 0 1389 1893 Size: 1389 504 NspI r_CATG'y Cuts at: 0 1110 1893 Size: 1110 783 PciI A'CATG_T Cuts at: 0 1106 1893 Size: 1106 787 PfoI T'CCnGG_A Cuts at: 0 536 707 740 1091 1893 Size: 536 171 33 351 802 Fragments arranged by size: 802 536 351 171 33 PpuMI rG'GwC_Cy Cuts at: 0 1035 1893 Size: 1035 858 PshAI GACnn'nnGTC Cuts at: 0 1504 1893 Size: 1504 389 PspOMI G'GGCC_C Cuts at: 0 26 40 1893 Size: 26 14 1853 Fragments arranged by size: 1853 26 14 PstI C_TGCA'G Cuts at: 0 1418 1596 1893 Size: 1418 178 297 Fragments arranged by size: 1418 297 178 SacI G_AGCT'C Cuts at: 0 644 1193 1893 Size: 644 549 700 Fragments arranged by size: 700 644 549 SapI GCTCTTCn'nnn_ Cuts at: 0 82 1893 Size: 82 1811 SbfI CC_TGCA'GG Cuts at: 0 1418 1893 Size: 1418 475 ScaI AGT'ACT Cuts at: 0 1354 1893 Size: 1354 539 SfcI C'TryA_G Cuts at: 0 683 1414 1592 1893 Size: 683 731 178 301 Fragments arranged by size: 731 683 301 178 SfoI GGC'GCC Cuts at: 0 1470 1893 Size: 1470 423 SmaI CCC'GGG Cuts at: 0 25 1893 Size: 25 1868 SmlI C'TyrA_G Cuts at: 0 880 1303 1330 1893 Size: 880 423 27 563 Fragments arranged by size: 880 563 423 27 SspI AAT'ATT Cuts at: 0 819 1893 Size: 819 1074 StyI C'CwwG_G Cuts at: 0 200 309 1601 1893 Size: 200 109 1292 292 Fragments arranged by size: 1292 292 200 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 451 1494 1795 1893 Size: 451 1043 301 98 Fragments arranged by size: 1043 451 301 98 TatI w'GTAC_w Cuts at: 0 1109 1352 1373 1646 1688 1893 Size: 1109 243 21 273 42 205 Fragments arranged by size: 1109 273 243 205 42 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1858 1890 1893 Size: 1858 32 3 Fragments arranged by size: 1858 32 3 XbaI T'CTAG_A Cuts at: 0 348 1893 Size: 348 1545 XmaI C'CCGG_G Cuts at: 0 23 1893 Size: 23 1870 ZraI GAC'GTC Cuts at: 0 1584 1893 Size: 1584 309 Enzymes that do cut: AatII AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI NaeI NarI NcoI NgoMIV NspI PciI PfoI PpuMI PshAI PspOMI PstI SacI SapI SbfI ScaI SfcI SfoI SmaI SmlI SspI StyI TaqII TatI TstI XbaI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AscI AseI AsiSI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsiWI BspEI BspMI BsrDI BssHII BstAPI BstZ17I ClaI CspCI DraI EciI EcoRI FseI FspAI HindIII HpaI KpnI MfeI MluI MspA1I NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI XhoI XmnI