Restriction map for
pF1KA1579
(Linear) (Six-base) MAPSORT of: KIAA1579.seq Check: 4870 from: 1 to: 2034
KIAA1579.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AccI GT'mk_AC
Cuts at: 0 615 2030 2034
Size: 615 1415 4
Fragments arranged by size:
1415 615 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 656 875 1279 1531 2034
Size: 394 262 219 404 252 503
Fragments arranged by size:
503 404 394 262 252 219
AflIII A'CryG_T
Cuts at: 0 972 2034
Size: 972 1062
AlwNI CAG_nnn'CTG
Cuts at: 0 242 1642 2034
Size: 242 1400 392
Fragments arranged by size:
1400 392 242
ApaI G_GGCC'C
Cuts at: 0 97 2034
Size: 97 1937
ApoI r'AATT_y
Cuts at: 0 749 1521 1688 1716 2034
Size: 749 772 167 28 318
Fragments arranged by size:
772 749 318 167 28
AseI AT'TA_AT
Cuts at: 0 1225 2034
Size: 1225 809
BanI G'GyrC_C
Cuts at: 0 118 2034
Size: 118 1916
BanII G_rGCy'C
Cuts at: 0 97 680 1280 2034
Size: 97 583 600 754
Fragments arranged by size:
754 600 583 97
BbeI G_GCGC'C
Cuts at: 0 122 2034
Size: 122 1912
BbvCI CC'TCA_GC
Cuts at: 0 99 2034
Size: 99 1935
BlpI GC'TnA_GC
Cuts at: 0 193 2034
Size: 193 1841
Bme1580I G_kGCm'C
Cuts at: 0 97 449 1472 1987 2034
Size: 97 352 1023 515 47
Fragments arranged by size:
1023 515 352 97 47
BmrI ACTGGGnnnn_n'
Cuts at: 0 710 1071 1730 2034
Size: 710 361 659 304
Fragments arranged by size:
710 659 361 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 947 2034
Size: 622 325 1087
Fragments arranged by size:
1087 622 325
Bpu10I CC'TnA_GC
Cuts at: 0 99 2034
Size: 99 1935
BsaBI GATnn'nnATC
Cuts at: 0 659 2034
Size: 659 1375
BsaHI Gr'CG_yC
Cuts at: 0 119 2034
Size: 119 1915
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 2034
Size: 169 1865
BseYI C'CCAG_C
Cuts at: 0 63 1281 1459 2034
Size: 63 1218 178 575
Fragments arranged by size:
1218 575 178 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1286 2034
Size: 130 1156 748
Fragments arranged by size:
1156 748 130
BsiHKAI G_wGCw'C
Cuts at: 0 680 924 2034
Size: 680 244 1110
Fragments arranged by size:
1110 680 244
BsmI GAATG_Cn'
Cuts at: 0 1799 1974 2034
Size: 1799 175 60
Fragments arranged by size:
1799 175 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 2034
Size: 11 2023
Bsp1286I G_dGCh'C
Cuts at: 0 97 449 680 924 1280 1472 1987 2034
Size: 97 352 231 244 356 192 515 47
Fragments arranged by size:
515 356 352 244 231 192 97 47
BspHI T'CATG_A
Cuts at: 0 255 2034
Size: 255 1779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 2034
Size: 233 1801
BsrDI GCAATG_nn'
Cuts at: 0 1445 1853 2034
Size: 1445 408 181
Fragments arranged by size:
1445 408 181
BssSI C'ACGA_G
Cuts at: 0 112 2034
Size: 112 1922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 640 2034
Size: 180 460 1394
Fragments arranged by size:
1394 460 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1084 2034
Size: 1084 950
BstZ17I GTA'TAC
Cuts at: 0 2031 2034
Size: 2031 3
Bsu36I CC'TnA_GG
Cuts at: 0 152 2034
Size: 152 1882
BtgI C'CryG_G
Cuts at: 0 1499 2034
Size: 1499 535
BtsI GCAGTG_nn'
Cuts at: 0 830 839 2034
Size: 830 9 1195
Fragments arranged by size:
1195 830 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 2034
Size: 400 1634
EaeI y'GGCC_r
Cuts at: 0 537 2034
Size: 537 1497
EarI CTCTTCn'nnn_
Cuts at: 0 461 731 851 1778 2034
Size: 461 270 120 927 256
Fragments arranged by size:
927 461 270 256 120
EcoICRI GAG'CTC
Cuts at: 0 678 2034
Size: 678 1356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 622 656 875 947 1279 1531 2034
Size: 394 228 34 219 72 332 252 503
Fragments arranged by size:
503 394 332 252 228 219 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 229 2034
Size: 229 1805
EcoO109I rG'GnC_Cy
Cuts at: 0 93 94 2034
Size: 93 1 1940
Fragments arranged by size:
1940 93 1
HaeII r_GCGC'y
Cuts at: 0 122 934 2034
Size: 122 812 1100
Fragments arranged by size:
1100 812 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 951 983 1374 1406 1798 1830
Size: 354 32 565 32 391 32 392 32
Cuts at: 1830 2034
Size: 204
Fragments arranged by size:
565 392 391 354 204 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1968 2034
Size: 1968 66
HindIII A'AGCT_T
Cuts at: 0 1575 2034
Size: 1575 459
KasI G'GCGC_C
Cuts at: 0 118 2034
Size: 118 1916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1411 2034
Size: 1411 623
MscI TGG'CCA
Cuts at: 0 539 2034
Size: 539 1495
MslI CAynn'nnrTG
Cuts at: 0 1352 2034
Size: 1352 682
MspA1I CmG'CkG
Cuts at: 0 177 186 1285 1408 2034
Size: 177 9 1099 123 626
Fragments arranged by size:
1099 626 177 123 9
NarI GG'CG_CC
Cuts at: 0 119 2034
Size: 119 1915
NspI r_CATG'y
Cuts at: 0 1422 2034
Size: 1422 612
PflMI CCAn_nnn'nTGG
Cuts at: 0 1873 1948 2034
Size: 1873 75 86
Fragments arranged by size:
1873 86 75
PfoI T'CCnGG_A
Cuts at: 0 924 2034
Size: 924 1110
PspOMI G'GGCC_C
Cuts at: 0 93 2034
Size: 93 1941
PstI C_TGCA'G
Cuts at: 0 176 2034
Size: 176 1858
PvuII CAG'CTG
Cuts at: 0 1285 1408 2034
Size: 1285 123 626
Fragments arranged by size:
1285 626 123
SacI G_AGCT'C
Cuts at: 0 680 2034
Size: 680 1354
SapI GCTCTTCn'nnn_
Cuts at: 0 731 2034
Size: 731 1303
SfcI C'TryA_G
Cuts at: 0 172 522 723 1069 2034
Size: 172 350 201 346 965
Fragments arranged by size:
965 350 346 201 172
SfoI GGC'GCC
Cuts at: 0 120 2034
Size: 120 1914
SspI AAT'ATT
Cuts at: 0 286 376 501 1242 2034
Size: 286 90 125 741 792
Fragments arranged by size:
792 741 286 125 90
TatI w'GTAC_w
Cuts at: 0 943 1020 2034
Size: 943 77 1014
Fragments arranged by size:
1014 943 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1506 2034
Size: 1506 528
XmnI GAAnn'nnTTC
Cuts at: 0 482 2034
Size: 482 1552
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AseI BanI
BanII BbeI BbvCI BlpI Bme1580I BmrI BpmI Bpu10I
BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstXI BstZ17I
Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NarI NspI PflMI PfoI PspOMI
PstI PvuII SacI SapI SfcI SfoI SspI TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaWI
BsaXI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstYI BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI