Restriction map for
pF1KSDA1563
(Linear) (Six-base) MAPSORT of: KIAA1563.seq Check: 523 from: 1 to: 4971
KIAA1563.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1687 4971
Size: 1687 3284
AatII G_ACGT'C
Cuts at: 0 3433 4971
Size: 3433 1538
AccI GT'mk_AC
Cuts at: 0 185 2736 3341 4971
Size: 185 2551 605 1630
Fragments arranged by size:
2551 1630 605 185
Acc65I G'GTAC_C
Cuts at: 0 523 4971
Size: 523 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 694 1273 2335 2915 4322 4879 4971
Size: 191 503 579 1062 580 1407 557 92
Fragments arranged by size:
1407 1062 580 579 557 503 191 92
AflII C'TTAA_G
Cuts at: 0 1485 4255 4714 4971
Size: 1485 2770 459 257
Fragments arranged by size:
2770 1485 459 257
AflIII A'CryG_T
Cuts at: 0 338 4971
Size: 338 4633
AgeI A'CCGG_T
Cuts at: 0 526 4971
Size: 526 4445
AhdI GACnn_n'nnGTC
Cuts at: 0 3980 4971
Size: 3980 991
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4088 4237 4971
Size: 4088 149 734
Fragments arranged by size:
4088 734 149
AlwNI CAG_nnn'CTG
Cuts at: 0 672 2601 4971
Size: 672 1929 2370
Fragments arranged by size:
2370 1929 672
ApaI G_GGCC'C
Cuts at: 0 3925 4971
Size: 3925 1046
ApoI r'AATT_y
Cuts at: 0 351 986 2172 2370 3670 4051 4262 4582
Size: 351 635 1186 198 1300 381 211 320
Cuts at: 4582 4845 4971
Size: 263 126
Fragments arranged by size:
1300 1186 635 381 351 320 263 211
198 126
AvrII C'CTAG_G
Cuts at: 0 1636 4971
Size: 1636 3335
BamHI G'GATC_C
Cuts at: 0 37 76 3120 4971
Size: 37 39 3044 1851
Fragments arranged by size:
3044 1851 39 37
BanI G'GyrC_C
Cuts at: 0 523 1240 3902 3986 4072 4971
Size: 523 717 2662 84 86 899
Fragments arranged by size:
2662 899 717 523 86 84
BanII G_rGCy'C
Cuts at: 0 1302 2766 3925 4971
Size: 1302 1464 1159 1046
Fragments arranged by size:
1464 1302 1159 1046
BbeI G_GCGC'C
Cuts at: 0 3906 3990 4971
Size: 3906 84 981
Fragments arranged by size:
3906 981 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 743 1446 1892 2695 3296 3400 4508 4781
Size: 743 703 446 803 601 104 1108 273
Cuts at: 4781 4971
Size: 190
Fragments arranged by size:
1108 803 743 703 601 446 273 190
104
BbvCI CC'TCA_GC
Cuts at: 0 1167 4659 4971
Size: 1167 3492 312
Fragments arranged by size:
3492 1167 312
BciVI GTATCCnnnnn_n'
Cuts at: 0 1872 4971
Size: 1872 3099
BclI T'GATC_A
Cuts at: 0 891 4971
Size: 891 4080
BglI GCCn_nnn'nGGC
Cuts at: 0 3911 4971
Size: 3911 1060
BglII A'GATC_T
Cuts at: 0 4742 4971
Size: 4742 229
BlpI GC'TnA_GC
Cuts at: 0 639 2405 4971
Size: 639 1766 2566
Fragments arranged by size:
2566 1766 639
Bme1580I G_kGCm'C
Cuts at: 0 1622 2182 3925 4971
Size: 1622 560 1743 1046
Fragments arranged by size:
1743 1622 1046 560
BmrI ACTGGGnnnn_n'
Cuts at: 0 556 2686 2832 3570 3919 4373 4521 4971
Size: 556 2130 146 738 349 454 148 450
Fragments arranged by size:
2130 738 556 454 450 349 148 146
BmtI G_CTAG'C
Cuts at: 0 2219 4971
Size: 2219 2752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4761 4971
Size: 4761 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1316 1711 1857 1958 2570 4971
Size: 228 1088 395 146 101 612 2401
Fragments arranged by size:
2401 1088 612 395 228 146 101
Bpu10I CC'TnA_GC
Cuts at: 0 1167 1301 4292 4659 4971
Size: 1167 134 2991 367 312
Fragments arranged by size:
2991 1167 367 312 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2739 4971
Size: 2739 2232
BsaI GGTCTCn'nnnn_
Cuts at: 0 547 1194 4971
Size: 547 647 3777
Fragments arranged by size:
3777 647 547
BsaAI yAC'GTr
Cuts at: 0 4194 4971
Size: 4194 777
BsaBI GATnn'nnATC
Cuts at: 0 3772 4386 4490 4971
Size: 3772 614 104 481
Fragments arranged by size:
3772 614 481 104
BsaHI Gr'CG_yC
Cuts at: 0 3430 3903 3987 4762 4971
Size: 3430 473 84 775 209
Fragments arranged by size:
3430 775 473 209 84
BsaWI w'CCGG_w
Cuts at: 0 526 4971
Size: 526 4445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 641 671 1433 1463 4231 4261 4971
Size: 641 30 762 30 2768 30 710
Fragments arranged by size:
2768 762 710 641 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 2953 4124 4322 4753 4971
Size: 490 2463 1171 198 431 218
Fragments arranged by size:
2463 1171 490 431 218 198
BseYI C'CCAG_C
Cuts at: 0 110 595 1614 2782 4971
Size: 110 485 1019 1168 2189
Fragments arranged by size:
2189 1168 1019 485 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 723 1481 4123 4594 4971
Size: 389 334 758 2642 471 377
Fragments arranged by size:
2642 758 471 389 377 334
BsiHKAI G_wGCw'C
Cuts at: 0 482 930 1302 4971
Size: 482 448 372 3669
Fragments arranged by size:
3669 482 448 372
BsmI GAATG_Cn'
Cuts at: 0 921 1776 2319 4971
Size: 921 855 543 2652
Fragments arranged by size:
2652 921 855 543
Bsp1286I G_dGCh'C
Cuts at: 0 482 930 1302 1622 2182 2766 3925 4971
Size: 482 448 372 320 560 584 1159 1046
Fragments arranged by size:
1159 1046 584 560 482 448 372 320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1687 4205 4971
Size: 1687 2518 766
Fragments arranged by size:
2518 1687 766
BsrDI GCAATG_nn'
Cuts at: 0 413 3284 4971
Size: 413 2871 1687
Fragments arranged by size:
2871 1687 413
BsrFI r'CCGG_y
Cuts at: 0 526 4971
Size: 526 4445
BsrGI T'GTAC_A
Cuts at: 0 648 4971
Size: 648 4323
BssSI C'ACGA_G
Cuts at: 0 2086 4971
Size: 2086 2885
BstAPI GCAn_nnn'nTGC
Cuts at: 0 390 701 925 1723 4971
Size: 390 311 224 798 3248
Fragments arranged by size:
3248 798 390 311 224
BstEII G'GTnAC_C
Cuts at: 0 1702 3570 4971
Size: 1702 1868 1401
Fragments arranged by size:
1868 1702 1401
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2304 4971
Size: 2304 2667
BstYI r'GATC_y
Cuts at: 0 37 76 669 3120 3760 4742 4875 4971
Size: 37 39 593 2451 640 982 133 96
Fragments arranged by size:
2451 982 640 593 133 96 39 37
Bsu36I CC'TnA_GG
Cuts at: 0 2936 4971
Size: 2936 2035
BtgI C'CryG_G
Cuts at: 0 4790 4971
Size: 4790 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 959 1640 4971
Size: 959 681 3331
Fragments arranged by size:
3331 959 681
BtsI GCAGTG_nn'
Cuts at: 0 320 551 818 1827 3069 3102 3863 4181
Size: 320 231 267 1009 1242 33 761 318
Cuts at: 4181 4971
Size: 790
Fragments arranged by size:
1242 1009 790 761 320 318 267 231
33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1382 1417 4971
Size: 1382 35 3554
Fragments arranged by size:
3554 1382 35
DraIII CAC_nnn'GTG
Cuts at: 0 2876 3097 4971
Size: 2876 221 1874
Fragments arranged by size:
2876 1874 221
DrdI GACnn_nn'nnGTC
Cuts at: 0 328 4971
Size: 328 4643
EaeI y'GGCC_r
Cuts at: 0 360 1914 2867 3456 4588 4971
Size: 360 1554 953 589 1132 383
Fragments arranged by size:
1554 1132 953 589 383 360
EarI CTCTTCn'nnn_
Cuts at: 0 7 206 2162 2335 3441 4971
Size: 7 199 1956 173 1106 1530
Fragments arranged by size:
1956 1530 1106 199 173 7
EcoICRI GAG'CTC
Cuts at: 0 1300 4971
Size: 1300 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 228 694 1273 1316 1711 1857 1958
Size: 191 37 466 579 43 395 146 101
Cuts at: 1958 2335 2570 2915 4322 4879 4971
Size: 377 235 345 1407 557 92
Fragments arranged by size:
1407 579 557 466 395 377 345 235
191 146 101 92 43 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1931 4304 4870 4971
Size: 1931 2373 566 101
Fragments arranged by size:
2373 1931 566 101
EcoO109I rG'GnC_Cy
Cuts at: 0 1923 3921 4971
Size: 1923 1998 1050
Fragments arranged by size:
1998 1923 1050
EcoRI G'AATT_C
Cuts at: 0 351 4051 4971
Size: 351 3700 920
Fragments arranged by size:
3700 920 351
EcoRV GAT'ATC
Cuts at: 0 1407 2133 3504 4971
Size: 1407 726 1371 1467
Fragments arranged by size:
1467 1407 1371 726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4370 4971
Size: 4370 601
HaeII r_GCGC'y
Cuts at: 0 3906 3990 4971
Size: 3906 84 981
Fragments arranged by size:
3906 981 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 41 73 780 812 937 969 1433 1465
Size: 41 32 707 32 125 32 464 32
Cuts at: 1465 2122 2154 2875 2907 3021 3053 3476 3508
Size: 657 32 721 32 114 32 423 32
Cuts at: 3508 3966 3998 4231 4263 4366 4398 4971
Size: 458 32 233 32 103 32 573
Fragments arranged by size:
721 707 657 573 464 458 423 233
125 114 103 41 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1768 2737 4971
Size: 1768 969 2234
Fragments arranged by size:
2234 1768 969
HindIII A'AGCT_T
Cuts at: 0 297 1996 2557 4963 4971
Size: 297 1699 561 2406 8
Fragments arranged by size:
2406 1699 561 297 8
KasI G'GCGC_C
Cuts at: 0 3902 3986 4971
Size: 3902 84 985
Fragments arranged by size:
3902 985 84
KpnI G_GTAC'C
Cuts at: 0 527 4971
Size: 527 4444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1813 4971
Size: 1813 3158
MscI TGG'CCA
Cuts at: 0 362 2869 3458 4590 4971
Size: 362 2507 589 1132 381
Fragments arranged by size:
2507 1132 589 381 362
MslI CAynn'nnrTG
Cuts at: 0 1625 3401 4070 4971
Size: 1625 1776 669 901
Fragments arranged by size:
1776 1625 901 669
MspA1I CmG'CkG
Cuts at: 0 1614 2199 3865 4272 4464 4971
Size: 1614 585 1666 407 192 507
Fragments arranged by size:
1666 1614 585 507 407 192
NarI GG'CG_CC
Cuts at: 0 3903 3987 4971
Size: 3903 84 984
Fragments arranged by size:
3903 984 84
NcoI C'CATG_G
Cuts at: 0 4790 4971
Size: 4790 181
NheI G'CTAG_C
Cuts at: 0 2215 4971
Size: 2215 2756
NruI TCG'CGA
Cuts at: 0 4496 4971
Size: 4496 475
NspI r_CATG'y
Cuts at: 0 342 2782 3225 4069 4940 4971
Size: 342 2440 443 844 871 31
Fragments arranged by size:
2440 871 844 443 342 31
PasI CC'CwG_GG
Cuts at: 0 4146 4971
Size: 4146 825
PciI A'CATG_T
Cuts at: 0 338 4971
Size: 338 4633
PflMI CCAn_nnn'nTGG
Cuts at: 0 2876 3300 4971
Size: 2876 424 1671
Fragments arranged by size:
2876 1671 424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2929 2961 4971
Size: 2929 32 2010
Fragments arranged by size:
2929 2010 32
PpuMI rG'GwC_Cy
Cuts at: 0 1923 4971
Size: 1923 3048
PspOMI G'GGCC_C
Cuts at: 0 3921 4971
Size: 3921 1050
PstI C_TGCA'G
Cuts at: 0 1851 2606 2777 4679 4971
Size: 1851 755 171 1902 292
Fragments arranged by size:
1902 1851 755 292 171
PvuII CAG'CTG
Cuts at: 0 1614 2199 3865 4272 4971
Size: 1614 585 1666 407 699
Fragments arranged by size:
1666 1614 699 585 407
SacI G_AGCT'C
Cuts at: 0 1302 4971
Size: 1302 3669
SalI G'TCGA_C
Cuts at: 0 2735 4971
Size: 2735 2236
ScaI AGT'ACT
Cuts at: 0 1979 4971
Size: 1979 2992
SfcI C'TryA_G
Cuts at: 0 186 1847 1928 1989 2099 2189 2602 2773
Size: 186 1661 81 61 110 90 413 171
Cuts at: 2773 3342 4675 4971
Size: 569 1333 296
Fragments arranged by size:
1661 1333 569 413 296 186 171 110
90 81 61
SfoI GGC'GCC
Cuts at: 0 3904 3988 4971
Size: 3904 84 983
Fragments arranged by size:
3904 983 84
SmlI C'TyrA_G
Cuts at: 0 1485 2718 4255 4714 4971
Size: 1485 1233 1537 459 257
Fragments arranged by size:
1537 1485 1233 459 257
SphI G_CATG'C
Cuts at: 0 2782 4971
Size: 2782 2189
SspI AAT'ATT
Cuts at: 0 3960 4111 4971
Size: 3960 151 860
Fragments arranged by size:
3960 860 151
StuI AGG'CCT
Cuts at: 0 53 2154 4123 4971
Size: 53 2101 1969 848
Fragments arranged by size:
2101 1969 848 53
StyI C'CwwG_G
Cuts at: 0 41 1636 2045 3930 4608 4790 4971
Size: 41 1595 409 1885 678 182 181
Fragments arranged by size:
1885 1595 678 409 182 181 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1039 3099 4971
Size: 1039 2060 1872
Fragments arranged by size:
2060 1872 1039
TatI w'GTAC_w
Cuts at: 0 648 1276 1977 4971
Size: 648 628 701 2994
Fragments arranged by size:
2994 701 648 628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3678 3710 4971
Size: 3678 32 1261
Fragments arranged by size:
3678 1261 32
XbaI T'CTAG_A
Cuts at: 0 249 4971
Size: 249 4722
XmnI GAAnn'nnTTC
Cuts at: 0 1130 2692 2803 4055 4971
Size: 1130 1562 111 1252 916
Fragments arranged by size:
1562 1252 1130 916 111
ZraI GAC'GTC
Cuts at: 0 3431 4971
Size: 3431 1540
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApoI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NheI NruI NspI PasI PciI PflMI PpiI
PpuMI PspOMI PstI PvuII SacI SalI ScaI SfcI
SfoI SmlI SphI SspI StuI StyI TaqII TatI
TstI XbaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AleI AloI ApaLI AscI AseI AsiSI
AvaI BaeI BcgI BfrBI BmgBI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BssHII BstBI BstZ17I ClaI DraI
EagI EciI FseI FspI FspAI HpaI MfeI MluI
NaeI NdeI NgoMIV NotI NsiI PacI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI Tth111I XcmI XhoI XmaI