Restriction map for
pF1KSDA1559
(Linear) (Six-base) MAPSORT of: KIAA1559.seq Check: 4595 from: 1 to: 1599
KIAA1559.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1599
Size: 325 1274
AgeI A'CCGG_T
Cuts at: 0 667 1599
Size: 667 932
AleI CACnn'nnGTG
Cuts at: 0 668 836 1340 1424 1599
Size: 668 168 504 84 175
Fragments arranged by size:
668 504 175 168 84
AlwNI CAG_nnn'CTG
Cuts at: 0 810 1599
Size: 810 789
ApaLI G'TGCA_C
Cuts at: 0 635 1599
Size: 635 964
ApoI r'AATT_y
Cuts at: 0 61 575 827 995 1499 1599
Size: 61 514 252 168 504 100
Fragments arranged by size:
514 504 252 168 100 61
BamHI G'GATC_C
Cuts at: 0 69 1599
Size: 69 1530
BbsI GAAGACnn'nnnn_
Cuts at: 0 541 1129 1213 1599
Size: 541 588 84 386
Fragments arranged by size:
588 541 386 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 830 1599
Size: 796 34 769
Fragments arranged by size:
796 769 34
Bme1580I G_kGCm'C
Cuts at: 0 639 1599
Size: 639 960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1599
Size: 254 1345
BsaI GGTCTCn'nnnn_
Cuts at: 0 1257 1599
Size: 1257 342
BsaWI w'CCGG_w
Cuts at: 0 667 1599
Size: 667 932
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 252 282 494 524 1599
Size: 252 30 212 30 1075
Fragments arranged by size:
1075 252 212 30 30
BsiHKAI G_wGCw'C
Cuts at: 0 639 719 1599
Size: 639 80 880
Fragments arranged by size:
880 639 80
BsmI GAATG_Cn'
Cuts at: 0 376 1247 1557 1599
Size: 376 871 310 42
Fragments arranged by size:
871 376 310 42
Bsp1286I G_dGCh'C
Cuts at: 0 639 719 1599
Size: 639 80 880
Fragments arranged by size:
880 639 80
BsrFI r'CCGG_y
Cuts at: 0 667 1599
Size: 667 932
BsrGI T'GTAC_A
Cuts at: 0 886 1599
Size: 886 713
BssSI C'ACGA_G
Cuts at: 0 1172 1599
Size: 1172 427
BstYI r'GATC_y
Cuts at: 0 69 1599
Size: 69 1530
BtgI C'CryG_G
Cuts at: 0 6 1599
Size: 6 1593
EaeI y'GGCC_r
Cuts at: 0 32 1599
Size: 32 1567
EarI CTCTTCn'nnn_
Cuts at: 0 1274 1599
Size: 1274 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 325 1599
Size: 254 71 1274
Fragments arranged by size:
1274 254 71
EcoO109I rG'GnC_Cy
Cuts at: 0 136 1599
Size: 136 1463
EcoRI G'AATT_C
Cuts at: 0 61 575 827 1499 1599
Size: 61 514 252 672 100
Fragments arranged by size:
672 514 252 100 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1563 1599
Size: 1563 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 1599
Size: 226 32 1341
Fragments arranged by size:
1341 226 32
HincII GTy'rAC
Cuts at: 0 565 1599
Size: 565 1034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 1599
Size: 1183 416
MscI TGG'CCA
Cuts at: 0 34 1599
Size: 34 1565
MslI CAynn'nnrTG
Cuts at: 0 303 584 668 752 836 1004 1088 1172
Size: 303 281 84 84 84 168 84 84
Cuts at: 1172 1256 1340 1424 1508 1599
Size: 84 84 84 84 91
Fragments arranged by size:
303 281 168 91 84 84 84 84
84 84 84 84 84
NcoI C'CATG_G
Cuts at: 0 6 1599
Size: 6 1593
PfoI T'CCnGG_A
Cuts at: 0 65 1599
Size: 65 1534
PpuMI rG'GwC_Cy
Cuts at: 0 136 1599
Size: 136 1463
PsiI TTA'TAA
Cuts at: 0 599 1599
Size: 599 1000
SfcI C'TryA_G
Cuts at: 0 114 333 1599
Size: 114 219 1266
Fragments arranged by size:
1266 219 114
SgrAI Cr'CCGG_yG
Cuts at: 0 667 1599
Size: 667 932
SpeI A'CTAG_T
Cuts at: 0 1146 1599
Size: 1146 453
StuI AGG'CCT
Cuts at: 0 622 706 790 1294 1546 1599
Size: 622 84 84 504 252 53
Fragments arranged by size:
622 504 252 84 84 53
StyI C'CwwG_G
Cuts at: 0 6 1599
Size: 6 1593
TatI w'GTAC_w
Cuts at: 0 798 886 984 1599
Size: 798 88 98 615
Fragments arranged by size:
798 615 98 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 494 526 1599
Size: 88 32 374 32 1073
Fragments arranged by size:
1073 374 88 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 958 1599
Size: 958 641
Enzymes that do cut:
AcuI AgeI AleI AlwNI ApaLI ApoI BamHI BbsI
BcgI Bme1580I BpmI BsaI BsaWI BsaXI BsiHKAI BsmI
Bsp1286I BsrFI BsrGI BssSI BstYI BtgI EaeI EarI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII MmeI MscI
MslI NcoI PfoI PpuMI PsiI SfcI SgrAI SpeI
StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SmaI SmlI SnaBI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI ZraI