Restriction map for
pF1KA1558
(Linear) (Six-base) MAPSORT of: KIAA1558.seq Check: 6178 from: 1 to: 2637
KIAA1558.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AatII G_ACGT'C
Cuts at: 0 1666 2637
Size: 1666 971
AccI GT'mk_AC
Cuts at: 0 449 1144 2485 2637
Size: 449 695 1341 152
Fragments arranged by size:
1341 695 449 152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 354 1973 2603 2637
Size: 91 263 1619 630 34
Fragments arranged by size:
1619 630 263 91 34
AflIII A'CryG_T
Cuts at: 0 511 1130 2637
Size: 511 619 1507
Fragments arranged by size:
1507 619 511
AleI CACnn'nnGTG
Cuts at: 0 2620 2637
Size: 2620 17
AlwNI CAG_nnn'CTG
Cuts at: 0 1951 2434 2637
Size: 1951 483 203
Fragments arranged by size:
1951 483 203
ApaI G_GGCC'C
Cuts at: 0 1426 2637
Size: 1426 1211
ApaLI G'TGCA_C
Cuts at: 0 1405 2637
Size: 1405 1232
ApoI r'AATT_y
Cuts at: 0 10 683 1178 2637
Size: 10 673 495 1459
Fragments arranged by size:
1459 673 495 10
AseI AT'TA_AT
Cuts at: 0 1297 2637
Size: 1297 1340
AvaI C'yCGr_G
Cuts at: 0 1917 2527 2578 2637
Size: 1917 610 51 59
Fragments arranged by size:
1917 610 59 51
BamHI G'GATC_C
Cuts at: 0 1008 2637
Size: 1008 1629
BanI G'GyrC_C
Cuts at: 0 2553 2637
Size: 2553 84
BanII G_rGCy'C
Cuts at: 0 704 1426 2637
Size: 704 722 1211
Fragments arranged by size:
1211 722 704
BbeI G_GCGC'C
Cuts at: 0 2557 2637
Size: 2557 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 939 994 1362 2174 2433 2637
Size: 939 55 368 812 259 204
Fragments arranged by size:
939 812 368 259 204 55
BbvCI CC'TCA_GC
Cuts at: 0 1346 2637
Size: 1346 1291
BciVI GTATCCnnnnn_n'
Cuts at: 0 239 2637
Size: 239 2398
BfrBI ATG'CAT
Cuts at: 0 631 2637
Size: 631 2006
BglI GCCn_nnn'nGGC
Cuts at: 0 875 2637
Size: 875 1762
BglII A'GATC_T
Cuts at: 0 863 1458 1539 2637
Size: 863 595 81 1098
Fragments arranged by size:
1098 863 595 81
BlpI GC'TnA_GC
Cuts at: 0 2404 2637
Size: 2404 233
Bme1580I G_kGCm'C
Cuts at: 0 1409 1426 2637
Size: 1409 17 1211
Fragments arranged by size:
1409 1211 17
BmgBI CAC'GTC
Cuts at: 0 512 2180 2637
Size: 512 1668 457
Fragments arranged by size:
1668 512 457
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 2261 2637
Size: 295 1966 376
Fragments arranged by size:
1966 376 295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2234 2637
Size: 2234 403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 2139 2204 2637
Size: 982 1157 65 433
Fragments arranged by size:
1157 982 433 65
Bpu10I CC'TnA_GC
Cuts at: 0 499 661 1346 2188 2637
Size: 499 162 685 842 449
Fragments arranged by size:
842 685 499 449 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 847 2477 2637
Size: 182 665 1630 160
Fragments arranged by size:
1630 665 182 160
BsaI GGTCTCn'nnnn_
Cuts at: 0 662 2637
Size: 662 1975
BsaHI Gr'CG_yC
Cuts at: 0 1663 2554 2637
Size: 1663 891 83
Fragments arranged by size:
1663 891 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 577 2143 2353 2533 2637
Size: 577 1566 210 180 104
Fragments arranged by size:
1566 577 210 180 104
BseYI C'CCAG_C
Cuts at: 0 1990 2156 2566 2637
Size: 1990 166 410 71
Fragments arranged by size:
1990 410 166 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1462 1482 2308 2637
Size: 1462 20 826 329
Fragments arranged by size:
1462 826 329 20
BsiHKAI G_wGCw'C
Cuts at: 0 1409 2091 2125 2194 2637
Size: 1409 682 34 69 443
Fragments arranged by size:
1409 682 443 69 34
BsmI GAATG_Cn'
Cuts at: 0 1885 2637
Size: 1885 752
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1483 2143 2637
Size: 1483 660 494
Fragments arranged by size:
1483 660 494
Bsp1286I G_dGCh'C
Cuts at: 0 704 1409 1426 2091 2125 2194 2637
Size: 704 705 17 665 34 69 443
Fragments arranged by size:
705 704 665 443 69 34 17
BspHI T'CATG_A
Cuts at: 0 948 2637
Size: 948 1689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1561 2637
Size: 1561 1076
BsrDI GCAATG_nn'
Cuts at: 0 1734 2637
Size: 1734 903
BstAPI GCAn_nnn'nTGC
Cuts at: 0 543 2637
Size: 543 2094
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2045 2637
Size: 2045 592
BstYI r'GATC_y
Cuts at: 0 863 940 1008 1458 1539 2107 2637
Size: 863 77 68 450 81 568 530
Fragments arranged by size:
863 568 530 450 81 77 68
BstZ17I GTA'TAC
Cuts at: 0 1145 2637
Size: 1145 1492
Bsu36I CC'TnA_GG
Cuts at: 0 1509 2637
Size: 1509 1128
BtgI C'CryG_G
Cuts at: 0 2082 2249 2315 2637
Size: 2082 167 66 322
Fragments arranged by size:
2082 322 167 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 587 1866 2637
Size: 587 1279 771
Fragments arranged by size:
1279 771 587
BtsI GCAGTG_nn'
Cuts at: 0 2001 2291 2313 2637
Size: 2001 290 22 324
Fragments arranged by size:
2001 324 290 22
DraI TTT'AAA
Cuts at: 0 1593 2637
Size: 1593 1044
DraIII CAC_nnn'GTG
Cuts at: 0 646 2611 2637
Size: 646 1965 26
Fragments arranged by size:
1965 646 26
EaeI y'GGCC_r
Cuts at: 0 2312 2592 2637
Size: 2312 280 45
Fragments arranged by size:
2312 280 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 354 982 1973 2139 2204 2603 2637
Size: 91 263 628 991 166 65 399 34
Fragments arranged by size:
991 628 399 263 166 91 65 34
EcoO109I rG'GnC_Cy
Cuts at: 0 1092 1422 1423 2637
Size: 1092 330 1 1214
Fragments arranged by size:
1214 1092 330 1
EcoRV GAT'ATC
Cuts at: 0 1052 2637
Size: 1052 1585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1995 2637
Size: 1995 642
HaeII r_GCGC'y
Cuts at: 0 2557 2637
Size: 2557 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 157 189 1231 1263 1732 1764
Size: 7 32 118 32 1042 32 469 32
Cuts at: 1764 1831 1863 2099 2131 2164 2196 2637
Size: 67 32 236 32 33 32 441
Fragments arranged by size:
1042 469 441 236 118 67 33 32
32 32 32 32 32 32 7
KasI G'GCGC_C
Cuts at: 0 2553 2637
Size: 2553 84
MscI TGG'CCA
Cuts at: 0 2314 2594 2637
Size: 2314 280 43
Fragments arranged by size:
2314 280 43
MslI CAynn'nnrTG
Cuts at: 0 626 2620 2637
Size: 626 1994 17
Fragments arranged by size:
1994 626 17
NarI GG'CG_CC
Cuts at: 0 2554 2637
Size: 2554 83
NcoI C'CATG_G
Cuts at: 0 2249 2315 2637
Size: 2249 66 322
Fragments arranged by size:
2249 322 66
NsiI A_TGCA'T
Cuts at: 0 633 2637
Size: 633 2004
NspI r_CATG'y
Cuts at: 0 1134 1811 2637
Size: 1134 677 826
Fragments arranged by size:
1134 826 677
PciI A'CATG_T
Cuts at: 0 1130 2637
Size: 1130 1507
PpuMI rG'GwC_Cy
Cuts at: 0 1092 2637
Size: 1092 1545
PshAI GACnn'nnGTC
Cuts at: 0 2437 2637
Size: 2437 200
PsiI TTA'TAA
Cuts at: 0 460 2637
Size: 460 2177
PspOMI G'GGCC_C
Cuts at: 0 1422 2637
Size: 1422 1215
PstI C_TGCA'G
Cuts at: 0 2538 2637
Size: 2538 99
SexAI A'CCwGG_T
Cuts at: 0 2607 2637
Size: 2607 30
SfcI C'TryA_G
Cuts at: 0 2414 2534 2637
Size: 2414 120 103
Fragments arranged by size:
2414 120 103
SfoI GGC'GCC
Cuts at: 0 2555 2637
Size: 2555 82
SmlI C'TyrA_G
Cuts at: 0 197 826 2492 2637
Size: 197 629 1666 145
Fragments arranged by size:
1666 629 197 145
SspI AAT'ATT
Cuts at: 0 762 1791 2637
Size: 762 1029 846
Fragments arranged by size:
1029 846 762
StuI AGG'CCT
Cuts at: 0 2562 2637
Size: 2562 75
StyI C'CwwG_G
Cuts at: 0 2249 2315 2637
Size: 2249 66 322
Fragments arranged by size:
2249 322 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 984 2055 2637
Size: 984 1071 582
Fragments arranged by size:
1071 984 582
TatI w'GTAC_w
Cuts at: 0 694 1924 1942 2353 2637
Size: 694 1230 18 411 284
Fragments arranged by size:
1230 694 411 284 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 2637
Size: 13 32 2592
Fragments arranged by size:
2592 32 13
Tth111I GACn'n_nGTC
Cuts at: 0 1471 2637
Size: 1471 1166
XbaI T'CTAG_A
Cuts at: 0 48 723 915 2637
Size: 48 675 192 1722
Fragments arranged by size:
1722 675 192 48
XmnI GAAnn'nnTTC
Cuts at: 0 593 653 1132 2637
Size: 593 60 479 1505
Fragments arranged by size:
1505 593 479 60
ZraI GAC'GTC
Cuts at: 0 1664 2637
Size: 1664 973
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII EaeI Eco57MI EcoO109I EcoRV FalI
HaeII Hin4I KasI MscI MslI NarI NcoI NsiI
NspI PciI PpuMI PshAI PsiI PspOMI PstI SexAI
SfcI SfoI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AsiSI AvrII BaeI BcgI BclI BmtI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII ClaI CspCI
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MspA1I NaeI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI XcmI XhoI XmaI