Restriction map for
pF1KA1539
(Linear) (Six-base) MAPSORT of: KIAA1539.seq Check: 6148 from: 1 to: 1614
KIAA1539.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 872 1614
Size: 872 742
AccI GT'mk_AC
Cuts at: 0 140 1614
Size: 140 1474
Acc65I G'GTAC_C
Cuts at: 0 172 1614
Size: 172 1442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 299 395 967 1614
Size: 256 43 96 572 647
Fragments arranged by size:
647 572 256 96 43
AflII C'TTAA_G
Cuts at: 0 1488 1614
Size: 1488 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 593 1614
Size: 478 115 1021
Fragments arranged by size:
1021 478 115
AlwNI CAG_nnn'CTG
Cuts at: 0 476 607 1614
Size: 476 131 1007
Fragments arranged by size:
1007 476 131
ApaI G_GGCC'C
Cuts at: 0 81 195 878 1614
Size: 81 114 683 736
Fragments arranged by size:
736 683 114 81
AvaI C'yCGr_G
Cuts at: 0 133 227 673 1614
Size: 133 94 446 941
Fragments arranged by size:
941 446 133 94
AvrII C'CTAG_G
Cuts at: 0 637 1614
Size: 637 977
BamHI G'GATC_C
Cuts at: 0 799 1614
Size: 799 815
BanI G'GyrC_C
Cuts at: 0 172 791 1273 1395 1614
Size: 172 619 482 122 219
Fragments arranged by size:
619 482 219 172 122
BanII G_rGCy'C
Cuts at: 0 81 195 302 601 878 939 958 997
Size: 81 114 107 299 277 61 19 39
Cuts at: 997 1474 1553 1614
Size: 477 79 61
Fragments arranged by size:
477 299 277 114 107 81 79 61
61 39 19
BbeI G_GCGC'C
Cuts at: 0 795 1614
Size: 795 819
BciVI GTATCCnnnnn_n'
Cuts at: 0 159 1614
Size: 159 1455
BlpI GC'TnA_GC
Cuts at: 0 952 991 1614
Size: 952 39 623
Fragments arranged by size:
952 623 39
Bme1580I G_kGCm'C
Cuts at: 0 81 195 668 878 1265 1276 1614
Size: 81 114 473 210 387 11 338
Fragments arranged by size:
473 387 338 210 114 81 11
BmgBI CAC'GTC
Cuts at: 0 1152 1614
Size: 1152 462
BmrI ACTGGGnnnn_n'
Cuts at: 0 486 595 995 1614
Size: 486 109 400 619
Fragments arranged by size:
619 486 400 109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 67 441 521 761 876 1555 1614
Size: 47 20 374 80 240 115 679 59
Fragments arranged by size:
679 374 240 115 80 59 47 20
Bpu10I CC'TnA_GC
Cuts at: 0 1479 1614
Size: 1479 135
BsaAI yAC'GTr
Cuts at: 0 9 1614
Size: 9 1605
BsaHI Gr'CG_yC
Cuts at: 0 792 1614
Size: 792 822
BsaWI w'CCGG_w
Cuts at: 0 971 1466 1614
Size: 971 495 148
Fragments arranged by size:
971 495 148
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 1236 1266 1614
Size: 466 30 740 30 348
Fragments arranged by size:
740 466 348 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1423 1429 1614
Size: 1423 6 185
Fragments arranged by size:
1423 185 6
BseYI C'CCAG_C
Cuts at: 0 111 252 324 607 1044 1145 1614
Size: 111 141 72 283 437 101 469
Fragments arranged by size:
469 437 283 141 111 101 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 532 1438 1614
Size: 31 501 906 176
Fragments arranged by size:
906 501 176 31
BsiHKAI G_wGCw'C
Cuts at: 0 680 1474 1614
Size: 680 794 140
Fragments arranged by size:
794 680 140
BsmBI CGTCTCn'nnnn_
Cuts at: 0 29 1614
Size: 29 1585
Bsp1286I G_dGCh'C
Cuts at: 0 81 195 302 601 668 680 878 939
Size: 81 114 107 299 67 12 198 61
Cuts at: 939 958 997 1265 1276 1474 1553 1614
Size: 19 39 268 11 198 79 61
Fragments arranged by size:
299 268 198 198 114 107 81 79
67 61 61 39 19 12 11
BspEI T'CCGG_A
Cuts at: 0 971 1466 1614
Size: 971 495 148
Fragments arranged by size:
971 495 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 872 1614
Size: 872 742
BsrBI CCG'CTC
Cuts at: 0 1478 1614
Size: 1478 136
BsrDI GCAATG_nn'
Cuts at: 0 624 1063 1614
Size: 624 439 551
Fragments arranged by size:
624 551 439
BstBI TT'CG_AA
Cuts at: 0 963 1614
Size: 963 651
BstYI r'GATC_y
Cuts at: 0 489 799 1227 1614
Size: 489 310 428 387
Fragments arranged by size:
489 428 387 310
Bsu36I CC'TnA_GG
Cuts at: 0 1459 1614
Size: 1459 155
BtgI C'CryG_G
Cuts at: 0 360 914 1614
Size: 360 554 700
Fragments arranged by size:
700 554 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1366 1614
Size: 1366 248
BtsI GCAGTG_nn'
Cuts at: 0 653 1614
Size: 653 961
EaeI y'GGCC_r
Cuts at: 0 338 1092 1614
Size: 338 754 522
Fragments arranged by size:
754 522 338
EarI CTCTTCn'nnn_
Cuts at: 0 232 764 1614
Size: 232 532 850
Fragments arranged by size:
850 532 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 936 1498 1614
Size: 31 905 562 116
Fragments arranged by size:
905 562 116 31
EcoICRI GAG'CTC
Cuts at: 0 1472 1614
Size: 1472 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 67 256 299 395 441 521 761
Size: 47 20 189 43 96 46 80 240
Cuts at: 761 876 967 1555 1614
Size: 115 91 588 59
Fragments arranged by size:
588 240 189 115 96 91 80 59
47 46 43 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1242 1614
Size: 973 269 372
Fragments arranged by size:
973 372 269
EcoO109I rG'GnC_Cy
Cuts at: 0 77 78 191 192 223 244 416 506
Size: 77 1 113 1 31 21 172 90
Cuts at: 506 706 1581 1614
Size: 200 875 33
Fragments arranged by size:
875 200 172 113 90 77 33 31
21 1 1
EcoRV GAT'ATC
Cuts at: 0 1506 1614
Size: 1506 108
HaeII r_GCGC'y
Cuts at: 0 795 1614
Size: 795 819
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 745 777 1614
Size: 466 32 247 32 837
Fragments arranged by size:
837 466 247 32 32
KasI G'GCGC_C
Cuts at: 0 791 1614
Size: 791 823
KpnI G_GTAC'C
Cuts at: 0 176 1614
Size: 176 1438
MscI TGG'CCA
Cuts at: 0 340 1094 1614
Size: 340 754 520
Fragments arranged by size:
754 520 340
MspA1I CmG'CkG
Cuts at: 0 546 1614
Size: 546 1068
NarI GG'CG_CC
Cuts at: 0 792 1614
Size: 792 822
NcoI C'CATG_G
Cuts at: 0 914 1614
Size: 914 700
NspI r_CATG'y
Cuts at: 0 1353 1614
Size: 1353 261
PasI CC'CwG_GG
Cuts at: 0 247 510 523 804 1614
Size: 247 263 13 281 810
Fragments arranged by size:
810 281 263 247 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 476 607 1311 1614
Size: 476 131 704 303
Fragments arranged by size:
704 476 303 131
PmlI CAC'GTG
Cuts at: 0 9 1614
Size: 9 1605
PpuMI rG'GwC_Cy
Cuts at: 0 223 506 1614
Size: 223 283 1108
Fragments arranged by size:
1108 283 223
PshAI GACnn'nnGTC
Cuts at: 0 504 908 1614
Size: 504 404 706
Fragments arranged by size:
706 504 404
PspOMI G'GGCC_C
Cuts at: 0 77 191 874 1614
Size: 77 114 683 740
Fragments arranged by size:
740 683 114 77
PstI C_TGCA'G
Cuts at: 0 1568 1614
Size: 1568 46
PvuII CAG'CTG
Cuts at: 0 546 1614
Size: 546 1068
SacI G_AGCT'C
Cuts at: 0 1474 1614
Size: 1474 140
SanDI GG'GwC_CC
Cuts at: 0 223 506 1614
Size: 223 283 1108
Fragments arranged by size:
1108 283 223
SexAI A'CCwGG_T
Cuts at: 0 143 1614
Size: 143 1471
SfcI C'TryA_G
Cuts at: 0 105 1564 1614
Size: 105 1459 50
Fragments arranged by size:
1459 105 50
SfoI GGC'GCC
Cuts at: 0 793 1614
Size: 793 821
SmaI CCC'GGG
Cuts at: 0 135 229 1614
Size: 135 94 1385
Fragments arranged by size:
1385 135 94
SmlI C'TyrA_G
Cuts at: 0 1488 1614
Size: 1488 126
StuI AGG'CCT
Cuts at: 0 986 1614
Size: 986 628
StyI C'CwwG_G
Cuts at: 0 637 914 1238 1265 1614
Size: 637 277 324 27 349
Fragments arranged by size:
637 349 324 277 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1393 1444 1591 1614
Size: 1393 51 147 23
Fragments arranged by size:
1393 147 51 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 207 239 320 352 1614
Size: 207 32 81 32 1262
Fragments arranged by size:
1262 207 81 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 868 1308 1614
Size: 868 440 306
Fragments arranged by size:
868 440 306
XmaI C'CCGG_G
Cuts at: 0 133 227 1614
Size: 133 94 1387
Fragments arranged by size:
1387 133 94
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AlfI AlwNI ApaI
AvaI AvrII BamHI BanI BanII BbeI BciVI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BstBI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I KasI KpnI MscI MspA1I NarI
NcoI NspI PasI PflMI PmlI PpuMI PshAI PspOMI
PstI PvuII SacI SanDI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI BaeI BbsI BbvCI
BcgI BclI BfrBI BglI BglII BmtI BplI BpuEI
BsaI BsaBI BsiEI BsiWI BsmI BspHI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoRI FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI