Restriction map for
pF1KSDA1537
(Linear) (Six-base) MAPSORT of: KIAA1537.seq Check: 7441 from: 1 to: 1770
KIAA1537.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1085 1211 1770
Size: 265 820 126 559
Fragments arranged by size:
820 559 265 126
AlwNI CAG_nnn'CTG
Cuts at: 0 1554 1770
Size: 1554 216
ApaI G_GGCC'C
Cuts at: 0 172 1770
Size: 172 1598
ApoI r'AATT_y
Cuts at: 0 1606 1652 1770
Size: 1606 46 118
Fragments arranged by size:
1606 118 46
BanII G_rGCy'C
Cuts at: 0 172 1770
Size: 172 1598
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 1526 1770
Size: 253 1273 244
Fragments arranged by size:
1273 253 244
BfrBI ATG'CAT
Cuts at: 0 1735 1770
Size: 1735 35
BglII A'GATC_T
Cuts at: 0 1335 1770
Size: 1335 435
BlpI GC'TnA_GC
Cuts at: 0 1495 1770
Size: 1495 275
Bme1580I G_kGCm'C
Cuts at: 0 172 1770
Size: 172 1598
BmtI G_CTAG'C
Cuts at: 0 910 1770
Size: 910 860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 979 1405 1770
Size: 688 291 426 365
Fragments arranged by size:
688 426 365 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1770
Size: 366 1404
BsaBI GATnn'nnATC
Cuts at: 0 437 1430 1755 1770
Size: 437 993 325 15
Fragments arranged by size:
993 437 325 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 209 239 1770
Size: 178 30 1 30 1531
Fragments arranged by size:
1531 178 30 30 1
BsmI GAATG_Cn'
Cuts at: 0 816 1770
Size: 816 954
Bsp1286I G_dGCh'C
Cuts at: 0 172 1770
Size: 172 1598
BspHI T'CATG_A
Cuts at: 0 1455 1770
Size: 1455 315
BsrDI GCAATG_nn'
Cuts at: 0 105 1543 1735 1770
Size: 105 1438 192 35
Fragments arranged by size:
1438 192 105 35
BsrGI T'GTAC_A
Cuts at: 0 842 1770
Size: 842 928
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1326 1770
Size: 1326 444
BstEII G'GTnAC_C
Cuts at: 0 774 1770
Size: 774 996
BstYI r'GATC_y
Cuts at: 0 231 339 1335 1770
Size: 231 108 996 435
Fragments arranged by size:
996 435 231 108
ClaI AT'CG_AT
Cuts at: 0 431 1770
Size: 431 1339
DraI TTT'AAA
Cuts at: 0 503 1770
Size: 503 1267
EaeI y'GGCC_r
Cuts at: 0 48 305 1770
Size: 48 257 1465
Fragments arranged by size:
1465 257 48
EarI CTCTTCn'nnn_
Cuts at: 0 662 1478 1770
Size: 662 816 292
Fragments arranged by size:
816 662 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 688 979 1085 1211 1405 1770
Size: 265 423 291 106 126 194 365
Fragments arranged by size:
423 365 291 265 194 126 106
EcoO109I rG'GnC_Cy
Cuts at: 0 168 169 1770
Size: 168 1 1601
Fragments arranged by size:
1601 168 1
EcoRI G'AATT_C
Cuts at: 0 1606 1770
Size: 1606 164
EcoRV GAT'ATC
Cuts at: 0 969 1770
Size: 969 801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1429 1770
Size: 1429 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 272 304 451 483 1345 1377
Size: 209 32 31 32 147 32 862 32
Cuts at: 1377 1770
Size: 393
Fragments arranged by size:
862 393 209 147 32 32 32 32
31
HindIII A'AGCT_T
Cuts at: 0 1492 1770
Size: 1492 278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 1770
Size: 456 1314
MslI CAynn'nnrTG
Cuts at: 0 1039 1770
Size: 1039 731
MspA1I CmG'CkG
Cuts at: 0 1146 1275 1770
Size: 1146 129 495
Fragments arranged by size:
1146 495 129
NheI G'CTAG_C
Cuts at: 0 906 1770
Size: 906 864
NsiI A_TGCA'T
Cuts at: 0 1737 1770
Size: 1737 33
PfoI T'CCnGG_A
Cuts at: 0 227 704 1421 1770
Size: 227 477 717 349
Fragments arranged by size:
717 477 349 227
PspOMI G'GGCC_C
Cuts at: 0 168 1770
Size: 168 1602
PstI C_TGCA'G
Cuts at: 0 1134 1247 1770
Size: 1134 113 523
Fragments arranged by size:
1134 523 113
PvuII CAG'CTG
Cuts at: 0 1146 1275 1770
Size: 1146 129 495
Fragments arranged by size:
1146 495 129
SapI GCTCTTCn'nnn_
Cuts at: 0 1478 1770
Size: 1478 292
SexAI A'CCwGG_T
Cuts at: 0 237 1770
Size: 237 1533
SfcI C'TryA_G
Cuts at: 0 1130 1243 1635 1770
Size: 1130 113 392 135
Fragments arranged by size:
1130 392 135 113
SmlI C'TyrA_G
Cuts at: 0 345 1770
Size: 345 1425
StuI AGG'CCT
Cuts at: 0 998 1770
Size: 998 772
TatI w'GTAC_w
Cuts at: 0 842 912 1770
Size: 842 70 858
Fragments arranged by size:
858 842 70
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 786 954 1143 1770
Size: 786 168 189 627
Fragments arranged by size:
786 627 189 168
XmnI GAAnn'nnTTC
Cuts at: 0 1337 1770
Size: 1337 433
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanII BbsI BfrBI BglII
BlpI Bme1580I BmtI BpmI BpuEI BsaBI BsaXI BsmI
Bsp1286I BspHI BsrDI BsrGI BstAPI BstEII BstYI ClaI
DraI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HindIII MmeI MslI MspA1I NheI NsiI PfoI
PspOMI PstI PvuII SapI SexAI SfcI SmlI StuI
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BglI BmgBI BmrI BplI Bpu10I
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
ZraI