Restriction map for
pF1KA1526
(Linear) (Six-base) MAPSORT of: KIAA1526.seq Check: 2160 from: 1 to: 2721
KIAA1526.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1304 2721
Size: 1304 1417
AatII G_ACGT'C
Cuts at: 0 1076 2204 2721
Size: 1076 1128 517
Fragments arranged by size:
1128 1076 517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 643 959 2721
Size: 444 199 316 1762
Fragments arranged by size:
1762 444 316 199
AfeI AGC'GCT
Cuts at: 0 134 2721
Size: 134 2587
AflII C'TTAA_G
Cuts at: 0 1036 2721
Size: 1036 1685
AflIII A'CryG_T
Cuts at: 0 500 2721
Size: 500 2221
AhdI GACnn_n'nnGTC
Cuts at: 0 21 547 861 2721
Size: 21 526 314 1860
Fragments arranged by size:
1860 526 314 21
AleI CACnn'nnGTG
Cuts at: 0 706 764 922 1102 2561 2721
Size: 706 58 158 180 1459 160
Fragments arranged by size:
1459 706 180 160 158 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2392 2721
Size: 2392 329
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1113 1145 2721
Size: 1113 32 1576
Fragments arranged by size:
1576 1113 32
AlwNI CAG_nnn'CTG
Cuts at: 0 564 937 1725 2095 2517 2721
Size: 564 373 788 370 422 204
Fragments arranged by size:
788 564 422 373 370 204
ApaI G_GGCC'C
Cuts at: 0 1222 2721
Size: 1222 1499
AvaI C'yCGr_G
Cuts at: 0 590 741 1531 1848 2721
Size: 590 151 790 317 873
Fragments arranged by size:
873 790 590 317 151
AvrII C'CTAG_G
Cuts at: 0 2521 2721
Size: 2521 200
BanI G'GyrC_C
Cuts at: 0 263 766 1049 1292 1441 1597 1894 2497
Size: 263 503 283 243 149 156 297 603
Cuts at: 2497 2721
Size: 224
Fragments arranged by size:
603 503 297 283 263 243 224 156
149
BanII G_rGCy'C
Cuts at: 0 34 58 478 631 959 1222 2435 2721
Size: 34 24 420 153 328 263 1213 286
Fragments arranged by size:
1213 420 328 286 263 153 34 24
BbeI G_GCGC'C
Cuts at: 0 267 2501 2721
Size: 267 2234 220
Fragments arranged by size:
2234 267 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 204 1851 2721
Size: 204 1647 870
Fragments arranged by size:
1647 870 204
BbvCI CC'TCA_GC
Cuts at: 0 1486 2721
Size: 1486 1235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 195 229 2469 2503 2721
Size: 195 34 2240 34 218
Fragments arranged by size:
2240 218 195 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 616 1226 1324 2005 2092 2721
Size: 616 610 98 681 87 629
Fragments arranged by size:
681 629 616 610 98 87
BlpI GC'TnA_GC
Cuts at: 0 148 2550 2721
Size: 148 2402 171
Fragments arranged by size:
2402 171 148
Bme1580I G_kGCm'C
Cuts at: 0 771 1222 1855 1897 2721
Size: 771 451 633 42 824
Fragments arranged by size:
824 771 633 451 42
BmgBI CAC'GTC
Cuts at: 0 2438 2721
Size: 2438 283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1834 2721
Size: 1834 887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1660 1804 2449 2721
Size: 1297 363 144 645 272
Fragments arranged by size:
1297 645 363 272 144
Bpu10I CC'TnA_GC
Cuts at: 0 1243 1486 2721
Size: 1243 243 1235
Fragments arranged by size:
1243 1235 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 2557 2721
Size: 943 1614 164
Fragments arranged by size:
1614 943 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 1105 1138 1563 2721
Size: 1105 33 425 1158
Fragments arranged by size:
1158 1105 425 33
BsaAI yAC'GTr
Cuts at: 0 501 2586 2721
Size: 501 2085 135
Fragments arranged by size:
2085 501 135
BsaHI Gr'CG_yC
Cuts at: 0 264 1073 1997 2201 2498 2721
Size: 264 809 924 204 297 223
Fragments arranged by size:
924 809 297 264 223 204
BsaWI w'CCGG_w
Cuts at: 0 252 791 2721
Size: 252 539 1930
Fragments arranged by size:
1930 539 252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1564 1585 1615 1963 1993 2422 2452
Size: 1534 30 21 30 348 30 429 30
Cuts at: 2452 2721
Size: 269
Fragments arranged by size:
1534 429 348 269 30 30 30 30
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 810 1294 1414 1908 2721
Size: 810 484 120 494 813
Fragments arranged by size:
813 810 494 484 120
BseYI C'CCAG_C
Cuts at: 0 51 752 849 1221 1537 1803 1975 2095
Size: 51 701 97 372 316 266 172 120
Cuts at: 2095 2252 2721
Size: 157 469
Fragments arranged by size:
701 469 372 316 266 172 157 120
97 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 790 1907 2721
Size: 109 681 1117 814
Fragments arranged by size:
1117 814 681 109
BsiEI CG_ry'CG
Cuts at: 0 65 332 861 2721
Size: 65 267 529 1860
Fragments arranged by size:
1860 529 267 65
BsiHKAI G_wGCw'C
Cuts at: 0 34 485 802 2630 2721
Size: 34 451 317 1828 91
Fragments arranged by size:
1828 451 317 91 34
BsiWI C'GTAC_G
Cuts at: 0 114 2721
Size: 114 2607
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1365 1965 2721
Size: 1365 600 756
Fragments arranged by size:
1365 756 600
Bsp1286I G_dGCh'C
Cuts at: 0 34 58 478 485 631 771 802 959
Size: 34 24 420 7 146 140 31 157
Cuts at: 959 1222 1855 1897 2435 2630 2721
Size: 263 633 42 538 195 91
Fragments arranged by size:
633 538 420 263 195 157 146 140
91 42 34 31 24 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 1304 2721
Size: 353 951 1417
Fragments arranged by size:
1417 951 353
BsrBI CCG'CTC
Cuts at: 0 162 2721
Size: 162 2559
BsrFI r'CCGG_y
Cuts at: 0 43 860 1225 2721
Size: 43 817 365 1496
Fragments arranged by size:
1496 817 365 43
BssHII G'CGCG_C
Cuts at: 0 199 2721
Size: 199 2522
BssSI C'ACGA_G
Cuts at: 0 445 1265 2721
Size: 445 820 1456
Fragments arranged by size:
1456 820 445
BstAPI GCAn_nnn'nTGC
Cuts at: 0 813 2721
Size: 813 1908
BstEII G'GTnAC_C
Cuts at: 0 594 787 1236 2721
Size: 594 193 449 1485
Fragments arranged by size:
1485 594 449 193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 2721
Size: 639 2082
BstYI r'GATC_y
Cuts at: 0 1708 2721
Size: 1708 1013
Bsu36I CC'TnA_GG
Cuts at: 0 772 2721
Size: 772 1949
BtgI C'CryG_G
Cuts at: 0 468 759 882 1148 1322 1347 1553 1593
Size: 468 291 123 266 174 25 206 40
Cuts at: 1593 1694 2041 2721
Size: 101 347 680
Fragments arranged by size:
680 468 347 291 266 206 174 123
101 40 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2466 2721
Size: 2466 255
BtsI GCAGTG_nn'
Cuts at: 0 177 1741 2721
Size: 177 1564 980
Fragments arranged by size:
1564 980 177
DraIII CAC_nnn'GTG
Cuts at: 0 489 1482 2721
Size: 489 993 1239
Fragments arranged by size:
1239 993 489
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 1024 2695 2721
Size: 253 771 1671 26
Fragments arranged by size:
1671 771 253 26
EaeI y'GGCC_r
Cuts at: 0 62 329 858 1619 2127 2468 2721
Size: 62 267 529 761 508 341 253
Fragments arranged by size:
761 529 508 341 267 253 62
EagI C'GGCC_G
Cuts at: 0 62 329 858 2721
Size: 62 267 529 1863
Fragments arranged by size:
1863 529 267 62
EarI CTCTTCn'nnn_
Cuts at: 0 319 1944 2721
Size: 319 1625 777
Fragments arranged by size:
1625 777 319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 172 1008 2721
Size: 172 836 1713
Fragments arranged by size:
1713 836 172
EcoICRI GAG'CTC
Cuts at: 0 32 2721
Size: 32 2689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 643 959 1297 1660 1804 2449 2721
Size: 444 199 316 338 363 144 645 272
Fragments arranged by size:
645 444 363 338 316 272 199 144
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1429 2721
Size: 1429 1292
EcoO109I rG'GnC_Cy
Cuts at: 0 336 1218 1371 1528 1815 1913 2721
Size: 336 882 153 157 287 98 808
Fragments arranged by size:
882 808 336 287 157 153 98
FspI TGC'GCA
Cuts at: 0 226 2721
Size: 226 2495
FspAI rTGC'GCAy
Cuts at: 0 226 2721
Size: 226 2495
HaeII r_GCGC'y
Cuts at: 0 136 267 2221 2324 2501 2721
Size: 136 131 1954 103 177 220
Fragments arranged by size:
1954 220 177 136 131 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 533 565 847 879 919 951
Size: 7 32 494 32 282 32 40 32
Cuts at: 951 1486 1518 1534 1566 1693 1725 1921 1953
Size: 535 32 16 32 127 32 196 32
Cuts at: 1953 2422 2454 2721
Size: 469 32 267
Fragments arranged by size:
535 494 469 282 267 196 127 40
32 32 32 32 32 32 32 32
32 16 7
HincII GTy'rAC
Cuts at: 0 573 2016 2721
Size: 573 1443 705
Fragments arranged by size:
1443 705 573
HindIII A'AGCT_T
Cuts at: 0 1731 2721
Size: 1731 990
KasI G'GCGC_C
Cuts at: 0 263 2497 2721
Size: 263 2234 224
Fragments arranged by size:
2234 263 224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 2721
Size: 326 2395
MscI TGG'CCA
Cuts at: 0 2129 2721
Size: 2129 592
MslI CAynn'nnrTG
Cuts at: 0 499 706 764 922 1102 2561 2721
Size: 499 207 58 158 180 1459 160
Fragments arranged by size:
1459 499 207 180 160 158 58
MspA1I CmG'CkG
Cuts at: 0 12 123 309 953 1891 1992 2267 2721
Size: 12 111 186 644 938 101 275 454
Fragments arranged by size:
938 644 454 275 186 111 101 12
NaeI GCC'GGC
Cuts at: 0 1227 2721
Size: 1227 1494
NarI GG'CG_CC
Cuts at: 0 264 2498 2721
Size: 264 2234 223
Fragments arranged by size:
2234 264 223
NcoI C'CATG_G
Cuts at: 0 1148 1322 1553 2721
Size: 1148 174 231 1168
Fragments arranged by size:
1168 1148 231 174
NgoMIV G'CCGG_C
Cuts at: 0 1225 2721
Size: 1225 1496
PasI CC'CwG_GG
Cuts at: 0 673 867 1719 1909 2475 2721
Size: 673 194 852 190 566 246
Fragments arranged by size:
852 673 566 246 194 190
PfoI T'CCnGG_A
Cuts at: 0 1132 2721
Size: 1132 1589
PmlI CAC'GTG
Cuts at: 0 2586 2721
Size: 2586 135
PpuMI rG'GwC_Cy
Cuts at: 0 1815 1913 2721
Size: 1815 98 808
Fragments arranged by size:
1815 808 98
PspOMI G'GGCC_C
Cuts at: 0 1218 2721
Size: 1218 1503
PstI C_TGCA'G
Cuts at: 0 2082 2721
Size: 2082 639
PvuII CAG'CTG
Cuts at: 0 123 309 2721
Size: 123 186 2412
Fragments arranged by size:
2412 186 123
SacI G_AGCT'C
Cuts at: 0 34 2721
Size: 34 2687
SapI GCTCTTCn'nnn_
Cuts at: 0 319 2721
Size: 319 2402
SexAI A'CCwGG_T
Cuts at: 0 218 842 1478 2721
Size: 218 624 636 1243
Fragments arranged by size:
1243 636 624 218
SfcI C'TryA_G
Cuts at: 0 363 1563 2078 2721
Size: 363 1200 515 643
Fragments arranged by size:
1200 643 515 363
SfoI GGC'GCC
Cuts at: 0 265 2499 2721
Size: 265 2234 222
Fragments arranged by size:
2234 265 222
SmaI CCC'GGG
Cuts at: 0 592 1533 2721
Size: 592 941 1188
Fragments arranged by size:
1188 941 592
SmlI C'TyrA_G
Cuts at: 0 958 1036 2572 2721
Size: 958 78 1536 149
Fragments arranged by size:
1536 958 149 78
StuI AGG'CCT
Cuts at: 0 2659 2721
Size: 2659 62
StyI C'CwwG_G
Cuts at: 0 437 903 1148 1322 1553 1767 1795 2521
Size: 437 466 245 174 231 214 28 726
Cuts at: 2521 2721
Size: 200
Fragments arranged by size:
726 466 437 245 231 214 200 174
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 696 1420 1917 2147 2721
Size: 190 506 724 497 230 574
Fragments arranged by size:
724 574 506 497 230 190
TatI w'GTAC_w
Cuts at: 0 653 2721
Size: 653 2068
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1585 1617 2721
Size: 1585 32 1104
Fragments arranged by size:
1585 1104 32
XbaI T'CTAG_A
Cuts at: 0 978 2721
Size: 978 1743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 282 760 1629 1989 2442 2721
Size: 48 234 478 869 360 453 279
Fragments arranged by size:
869 478 453 360 279 234 48
XmaI C'CCGG_G
Cuts at: 0 590 1531 2721
Size: 590 941 1190
Fragments arranged by size:
1190 941 590
ZraI GAC'GTC
Cuts at: 0 1074 2202 2721
Size: 1074 1128 519
Fragments arranged by size:
1128 1074 519
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BciVI BclI BfrBI BglII BmrI
BmtI BsaBI BsmI BspEI BspHI BsrDI BsrGI BstBI
BstZ17I ClaI CspCI DraI EcoRI EcoRV FalI FseI
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XhoI XmnI