Restriction map for
pF1KSDA1499
(Linear) (Six-base) MAPSORT of: KIAA1499.seq Check: 1074 from: 1 to: 1851
KIAA1499.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1776 1851
Size: 1776 75
AatII G_ACGT'C
Cuts at: 0 274 1851
Size: 274 1577
AccI GT'mk_AC
Cuts at: 0 1286 1851
Size: 1286 565
Acc65I G'GTAC_C
Cuts at: 0 967 1151 1851
Size: 967 184 700
Fragments arranged by size:
967 700 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 899 1851
Size: 824 75 952
Fragments arranged by size:
952 824 75
AflIII A'CryG_T
Cuts at: 0 482 1058 1851
Size: 482 576 793
Fragments arranged by size:
793 576 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1594 1790 1851
Size: 1594 196 61
Fragments arranged by size:
1594 196 61
ApaI G_GGCC'C
Cuts at: 0 254 1851
Size: 254 1597
ApaLI G'TGCA_C
Cuts at: 0 415 1851
Size: 415 1436
AvaI C'yCGr_G
Cuts at: 0 237 463 1851
Size: 237 226 1388
Fragments arranged by size:
1388 237 226
BamHI G'GATC_C
Cuts at: 0 867 1090 1374 1851
Size: 867 223 284 477
Fragments arranged by size:
867 477 284 223
BanI G'GyrC_C
Cuts at: 0 587 967 1151 1694 1752 1788 1851
Size: 587 380 184 543 58 36 63
Fragments arranged by size:
587 543 380 184 63 58 36
BanII G_rGCy'C
Cuts at: 0 254 1616 1851
Size: 254 1362 235
Fragments arranged by size:
1362 254 235
BbvCI CC'TCA_GC
Cuts at: 0 340 1851
Size: 340 1511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 911 1851
Size: 877 34 940
Fragments arranged by size:
940 877 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1497 1851
Size: 1497 354
BclI T'GATC_A
Cuts at: 0 330 1851
Size: 330 1521
BglI GCCn_nnn'nGGC
Cuts at: 0 395 1851
Size: 395 1456
Bme1580I G_kGCm'C
Cuts at: 0 254 419 590 1757 1851
Size: 254 165 171 1167 94
Fragments arranged by size:
1167 254 171 165 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 568 741 1058 1606 1851
Size: 536 32 173 317 548 245
Fragments arranged by size:
548 536 317 245 173 32
Bpu10I CC'TnA_GC
Cuts at: 0 340 1851
Size: 340 1511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1851
Size: 883 968
BsaI GGTCTCn'nnnn_
Cuts at: 0 368 1851
Size: 368 1483
BsaAI yAC'GTr
Cuts at: 0 1260 1837 1851
Size: 1260 577 14
Fragments arranged by size:
1260 577 14
BsaHI Gr'CG_yC
Cuts at: 0 271 1214 1851
Size: 271 943 637
Fragments arranged by size:
943 637 271
BsaWI w'CCGG_w
Cuts at: 0 157 503 1851
Size: 157 346 1348
Fragments arranged by size:
1348 346 157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1806 1836 1851
Size: 1806 30 15
Fragments arranged by size:
1806 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 165 1624 1851
Size: 165 1459 227
Fragments arranged by size:
1459 227 165
BseYI C'CCAG_C
Cuts at: 0 247 1310 1705 1770 1851
Size: 247 1063 395 65 81
Fragments arranged by size:
1063 395 247 81 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1686 1714 1851
Size: 1686 28 137
Fragments arranged by size:
1686 137 28
BsiHKAI G_wGCw'C
Cuts at: 0 419 776 1616 1851
Size: 419 357 840 235
Fragments arranged by size:
840 419 357 235
BsmBI CGTCTCn'nnnn_
Cuts at: 0 262 951 1851
Size: 262 689 900
Fragments arranged by size:
900 689 262
Bsp1286I G_dGCh'C
Cuts at: 0 254 419 590 776 1616 1757 1851
Size: 254 165 171 186 840 141 94
Fragments arranged by size:
840 254 186 171 165 141 94
BspEI T'CCGG_A
Cuts at: 0 503 1851
Size: 503 1348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1776 1851
Size: 1776 75
BsrDI GCAATG_nn'
Cuts at: 0 1805 1851
Size: 1805 46
BsrFI r'CCGG_y
Cuts at: 0 1064 1746 1851
Size: 1064 682 105
Fragments arranged by size:
1064 682 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1260 1851
Size: 1260 591
BstEII G'GTnAC_C
Cuts at: 0 1539 1851
Size: 1539 312
BstXI CCAn_nnnn'nTGG
Cuts at: 0 124 315 745 1837 1851
Size: 124 191 430 1092 14
Fragments arranged by size:
1092 430 191 124 14
BstYI r'GATC_y
Cuts at: 0 867 1090 1374 1851
Size: 867 223 284 477
Fragments arranged by size:
867 477 284 223
BtgI C'CryG_G
Cuts at: 0 393 595 1700 1851
Size: 393 202 1105 151
Fragments arranged by size:
1105 393 202 151
BtsI GCAGTG_nn'
Cuts at: 0 417 1025 1115 1254 1648 1674 1741 1851
Size: 417 608 90 139 394 26 67 110
Fragments arranged by size:
608 417 394 139 110 90 67 26
DraIII CAC_nnn'GTG
Cuts at: 0 1701 1851
Size: 1701 150
DrdI GACnn_nn'nnGTC
Cuts at: 0 1135 1851
Size: 1135 716
EaeI y'GGCC_r
Cuts at: 0 2 598 1463 1851
Size: 2 596 865 388
Fragments arranged by size:
865 596 388 2
EarI CTCTTCn'nnn_
Cuts at: 0 1010 1624 1851
Size: 1010 614 227
Fragments arranged by size:
1010 614 227
EcoICRI GAG'CTC
Cuts at: 0 1614 1851
Size: 1614 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 568 741 824 899 1058 1606 1851
Size: 536 32 173 83 75 159 548 245
Fragments arranged by size:
548 536 245 173 159 83 75 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 916 1851
Size: 916 935
EcoO109I rG'GnC_Cy
Cuts at: 0 251 1851
Size: 251 1600
HaeII r_GCGC'y
Cuts at: 0 305 635 1851
Size: 305 330 1216
Fragments arranged by size:
1216 330 305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 931 963 1495 1527 1806 1838 1851
Size: 931 32 532 32 279 32 13
Fragments arranged by size:
931 532 279 32 32 32 13
KpnI G_GTAC'C
Cuts at: 0 971 1155 1851
Size: 971 184 696
Fragments arranged by size:
971 696 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1851
Size: 203 1648
MscI TGG'CCA
Cuts at: 0 1465 1851
Size: 1465 386
MslI CAynn'nnrTG
Cuts at: 0 384 1851
Size: 384 1467
MspA1I CmG'CkG
Cuts at: 0 560 1314 1662 1774 1851
Size: 560 754 348 112 77
Fragments arranged by size:
754 560 348 112 77
NaeI GCC'GGC
Cuts at: 0 1066 1851
Size: 1066 785
NgoMIV G'CCGG_C
Cuts at: 0 1064 1851
Size: 1064 787
NspI r_CATG'y
Cuts at: 0 486 667 1062 1851
Size: 486 181 395 789
Fragments arranged by size:
789 486 395 181
PciI A'CATG_T
Cuts at: 0 482 1058 1851
Size: 482 576 793
Fragments arranged by size:
793 576 482
PflMI CCAn_nnn'nTGG
Cuts at: 0 1461 1851
Size: 1461 390
PmlI CAC'GTG
Cuts at: 0 1837 1851
Size: 1837 14
PsiI TTA'TAA
Cuts at: 0 1277 1851
Size: 1277 574
PspOMI G'GGCC_C
Cuts at: 0 250 1851
Size: 250 1601
PspXI vC'TCGA_Gb
Cuts at: 0 237 1851
Size: 237 1614
PstI C_TGCA'G
Cuts at: 0 1032 1110 1565 1851
Size: 1032 78 455 286
Fragments arranged by size:
1032 455 286 78
PvuII CAG'CTG
Cuts at: 0 560 1314 1662 1774 1851
Size: 560 754 348 112 77
Fragments arranged by size:
754 560 348 112 77
SacI G_AGCT'C
Cuts at: 0 1616 1851
Size: 1616 235
SexAI A'CCwGG_T
Cuts at: 0 1154 1851
Size: 1154 697
SfcI C'TryA_G
Cuts at: 0 978 1028 1106 1115 1561 1851
Size: 978 50 78 9 446 290
Fragments arranged by size:
978 446 290 78 50 9
SmlI C'TyrA_G
Cuts at: 0 237 463 862 1851
Size: 237 226 399 989
Fragments arranged by size:
989 399 237 226
StyI C'CwwG_G
Cuts at: 0 789 1235 1370 1851
Size: 789 446 135 481
Fragments arranged by size:
789 481 446 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1324 1426 1784 1851
Size: 1324 102 358 67
Fragments arranged by size:
1324 358 102 67
TatI w'GTAC_w
Cuts at: 0 613 656 1851
Size: 613 43 1195
Fragments arranged by size:
1195 613 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1436 1468 1851
Size: 1436 32 383
Fragments arranged by size:
1436 383 32
Tth111I GACn'n_nGTC
Cuts at: 0 943 1851
Size: 943 908
XbaI T'CTAG_A
Cuts at: 0 429 1851
Size: 429 1422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 34 1170 1778 1851
Size: 34 1136 608 73
Fragments arranged by size:
1136 608 73 34
XhoI C'TCGA_G
Cuts at: 0 237 463 1851
Size: 237 226 1388
Fragments arranged by size:
1388 237 226
ZraI GAC'GTC
Cuts at: 0 272 1851
Size: 272 1579
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AlfI ApaI
ApaLI AvaI BamHI BanI BanII BbvCI BcgI BciVI
BclI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtsI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PciI PflMI PmlI
PsiI PspOMI PspXI PstI PvuII SacI SexAI SfcI
SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AloI AlwNI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaBI
BsiEI BsiWI BsmI BspHI BsrBI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PpiI PpuMI PshAI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XmaI XmnI