Restriction map for
pF1KSDA1486
(Linear) (Six-base) MAPSORT of: KIAA1486.seq Check: 4325 from: 1 to: 1959
KIAA1486.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AatII G_ACGT'C
Cuts at: 0 661 1870 1959
Size: 661 1209 89
Fragments arranged by size:
1209 661 89
AccI GT'mk_AC
Cuts at: 0 800 1252 1773 1864 1959
Size: 800 452 521 91 95
Fragments arranged by size:
800 521 452 95 91
Acc65I G'GTAC_C
Cuts at: 0 1932 1959
Size: 1932 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 750 1394 1959
Size: 539 211 644 565
Fragments arranged by size:
644 565 539 211
AflIII A'CryG_T
Cuts at: 0 1103 1959
Size: 1103 856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1588 1933 1959
Size: 1588 345 26
Fragments arranged by size:
1588 345 26
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1257 1289 1622 1654 1959
Size: 1257 32 333 32 305
Fragments arranged by size:
1257 333 305 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 360 395 1959
Size: 360 35 1564
Fragments arranged by size:
1564 360 35
ApaI G_GGCC'C
Cuts at: 0 650 1959
Size: 650 1309
AvaI C'yCGr_G
Cuts at: 0 128 712 1494 1959
Size: 128 584 782 465
Fragments arranged by size:
782 584 465 128
BanI G'GyrC_C
Cuts at: 0 900 1932 1959
Size: 900 1032 27
Fragments arranged by size:
1032 900 27
BanII G_rGCy'C
Cuts at: 0 650 728 1405 1528 1798 1959
Size: 650 78 677 123 270 161
Fragments arranged by size:
677 650 270 161 123 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 44 204 1697 1959
Size: 44 160 1493 262
Fragments arranged by size:
1493 262 160 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 600 634 1959
Size: 600 34 1325
Fragments arranged by size:
1325 600 34
BlpI GC'TnA_GC
Cuts at: 0 451 607 1959
Size: 451 156 1352
Fragments arranged by size:
1352 451 156
Bme1580I G_kGCm'C
Cuts at: 0 379 650 1959
Size: 379 271 1309
Fragments arranged by size:
1309 379 271
BmgBI CAC'GTC
Cuts at: 0 1158 1959
Size: 1158 801
BmrI ACTGGGnnnn_n'
Cuts at: 0 1263 1959
Size: 1263 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 315 1093 1198 1552 1695 1959
Size: 43 272 778 105 354 143 264
Fragments arranged by size:
778 354 272 264 143 105 43
Bpu10I CC'TnA_GC
Cuts at: 0 1345 1730 1959
Size: 1345 385 229
Fragments arranged by size:
1345 385 229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 1380 1959
Size: 165 1215 579
Fragments arranged by size:
1215 579 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 701 1959
Size: 701 1258
BsaAI yAC'GTr
Cuts at: 0 261 1959
Size: 261 1698
BsaHI Gr'CG_yC
Cuts at: 0 658 845 1132 1702 1867 1959
Size: 658 187 287 570 165 92
Fragments arranged by size:
658 570 287 187 165 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 1257 1287 1959
Size: 715 30 512 30 672
Fragments arranged by size:
715 672 512 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 293 563 666 736 1221 1545 1549 1871
Size: 293 270 103 70 485 324 4 322
Cuts at: 1871 1959
Size: 88
Fragments arranged by size:
485 324 322 293 270 103 88 70
4
BseYI C'CCAG_C
Cuts at: 0 439 1959
Size: 439 1520
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1959
Size: 346 1613
BsiEI CG_ry'CG
Cuts at: 0 1563 1959
Size: 1563 396
BsiHKAI G_wGCw'C
Cuts at: 0 457 613 1405 1959
Size: 457 156 792 554
Fragments arranged by size:
792 554 457 156
BsmI GAATG_Cn'
Cuts at: 0 117 1380 1959
Size: 117 1263 579
Fragments arranged by size:
1263 579 117
Bsp1286I G_dGCh'C
Cuts at: 0 379 457 613 650 728 1405 1528 1798
Size: 379 78 156 37 78 677 123 270
Cuts at: 1798 1959
Size: 161
Fragments arranged by size:
677 379 270 161 156 123 78 78
37
BspHI T'CATG_A
Cuts at: 0 287 1959
Size: 287 1672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 992 1215 1925 1959
Size: 992 223 710 34
Fragments arranged by size:
992 710 223 34
BsrBI CCG'CTC
Cuts at: 0 497 1959
Size: 497 1462
BsrDI GCAATG_nn'
Cuts at: 0 1032 1959
Size: 1032 927
BsrFI r'CCGG_y
Cuts at: 0 1592 1853 1959
Size: 1592 261 106
Fragments arranged by size:
1592 261 106
BsrGI T'GTAC_A
Cuts at: 0 731 1959
Size: 731 1228
BssSI C'ACGA_G
Cuts at: 0 1521 1959
Size: 1521 438
BstEII G'GTnAC_C
Cuts at: 0 1764 1959
Size: 1764 195
Bsu36I CC'TnA_GG
Cuts at: 0 32 305 1900 1959
Size: 32 273 1595 59
Fragments arranged by size:
1595 273 59 32
BtgI C'CryG_G
Cuts at: 0 667 701 896 1193 1278 1469 1695 1959
Size: 667 34 195 297 85 191 226 264
Fragments arranged by size:
667 297 264 226 195 191 85 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 563 1959
Size: 563 1396
BtsI GCAGTG_nn'
Cuts at: 0 479 889 1917 1959
Size: 479 410 1028 42
Fragments arranged by size:
1028 479 410 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 522 1959
Size: 522 1437
EaeI y'GGCC_r
Cuts at: 0 1164 1560 1590 1959
Size: 1164 396 30 369
Fragments arranged by size:
1164 396 369 30
EagI C'GGCC_G
Cuts at: 0 1560 1959
Size: 1560 399
EarI CTCTTCn'nnn_
Cuts at: 0 713 1555 1959
Size: 713 842 404
Fragments arranged by size:
842 713 404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 959 1959
Size: 959 1000
EcoICRI GAG'CTC
Cuts at: 0 1403 1959
Size: 1403 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 315 539 750 1093 1198 1394 1552
Size: 43 272 224 211 343 105 196 158
Cuts at: 1552 1695 1959
Size: 143 264
Fragments arranged by size:
343 272 264 224 211 196 158 143
105 43
EcoO109I rG'GnC_Cy
Cuts at: 0 435 647 1204 1331 1959
Size: 435 212 557 127 628
Fragments arranged by size:
628 557 435 212 127
EcoRV GAT'ATC
Cuts at: 0 5 1959
Size: 5 1954
FspI TGC'GCA
Cuts at: 0 1576 1959
Size: 1576 383
HaeII r_GCGC'y
Cuts at: 0 1599 1959
Size: 1599 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 558 590 1706 1738 1959
Size: 12 32 514 32 1116 32 221
Fragments arranged by size:
1116 514 221 32 32 32 12
HincII GTy'rAC
Cuts at: 0 1464 1774 1865 1959
Size: 1464 310 91 94
Fragments arranged by size:
1464 310 94 91
HindIII A'AGCT_T
Cuts at: 0 1084 1959
Size: 1084 875
KpnI G_GTAC'C
Cuts at: 0 1936 1959
Size: 1936 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 1652 1959
Size: 1073 579 307
Fragments arranged by size:
1073 579 307
MslI CAynn'nnrTG
Cuts at: 0 292 548 739 1959
Size: 292 256 191 1220
Fragments arranged by size:
1220 292 256 191
MspA1I CmG'CkG
Cuts at: 0 395 606 669 703 872 1005 1195 1471
Size: 395 211 63 34 169 133 190 276
Cuts at: 1471 1530 1959
Size: 59 429
Fragments arranged by size:
429 395 276 211 190 169 133 63
59 34
NaeI GCC'GGC
Cuts at: 0 1594 1959
Size: 1594 365
NcoI C'CATG_G
Cuts at: 0 1278 1695 1959
Size: 1278 417 264
Fragments arranged by size:
1278 417 264
NgoMIV G'CCGG_C
Cuts at: 0 1592 1959
Size: 1592 367
PasI CC'CwG_GG
Cuts at: 0 1200 1959
Size: 1200 759
PfoI T'CCnGG_A
Cuts at: 0 1510 1959
Size: 1510 449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1257 1289 1959
Size: 1257 32 670
Fragments arranged by size:
1257 670 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 1204 1331 1959
Size: 435 769 127 628
Fragments arranged by size:
769 628 435 127
PshAI GACnn'nnGTC
Cuts at: 0 1437 1959
Size: 1437 522
PspOMI G'GGCC_C
Cuts at: 0 646 1959
Size: 646 1313
PstI C_TGCA'G
Cuts at: 0 486 1614 1959
Size: 486 1128 345
Fragments arranged by size:
1128 486 345
PvuII CAG'CTG
Cuts at: 0 395 606 872 1959
Size: 395 211 266 1087
Fragments arranged by size:
1087 395 266 211
SacI G_AGCT'C
Cuts at: 0 1405 1959
Size: 1405 554
SacII CC_GC'GG
Cuts at: 0 670 704 1196 1472 1959
Size: 670 34 492 276 487
Fragments arranged by size:
670 492 487 276 34
SalI G'TCGA_C
Cuts at: 0 1772 1863 1959
Size: 1772 91 96
Fragments arranged by size:
1772 96 91
SanDI GG'GwC_CC
Cuts at: 0 435 1959
Size: 435 1524
SfcI C'TryA_G
Cuts at: 0 482 1610 1959
Size: 482 1128 349
Fragments arranged by size:
1128 482 349
SmaI CCC'GGG
Cuts at: 0 1496 1959
Size: 1496 463
SmlI C'TyrA_G
Cuts at: 0 128 144 1395 1959
Size: 128 16 1251 564
Fragments arranged by size:
1251 564 128 16
SspI AAT'ATT
Cuts at: 0 556 1959
Size: 556 1403
StuI AGG'CCT
Cuts at: 0 88 925 1359 1959
Size: 88 837 434 600
Fragments arranged by size:
837 600 434 88
StyI C'CwwG_G
Cuts at: 0 920 944 1278 1695 1959
Size: 920 24 334 417 264
Fragments arranged by size:
920 417 334 264 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 1533 1959
Size: 435 1098 426
Fragments arranged by size:
1098 435 426
TatI w'GTAC_w
Cuts at: 0 62 731 1959
Size: 62 669 1228
Fragments arranged by size:
1228 669 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 245 277 1071 1103 1959
Size: 245 32 794 32 856
Fragments arranged by size:
856 794 245 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1276 1527 1959
Size: 1276 251 432
Fragments arranged by size:
1276 432 251
XhoI C'TCGA_G
Cuts at: 0 128 1959
Size: 128 1831
XmaI C'CCGG_G
Cuts at: 0 1494 1959
Size: 1494 465
ZraI GAC'GTC
Cuts at: 0 659 1868 1959
Size: 659 1209 91
Fragments arranged by size:
1209 659 91
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI AvaI BanI BanII BbsI BcgI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I
HincII HindIII KpnI MmeI MslI MspA1I NaeI NcoI
NgoMIV PasI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SalI SanDI SfcI SmaI SmlI
SspI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BmtI BplI
BsaBI BsaWI BsiWI BsmBI BspEI BssHII BstAPI BstBI
BstXI BstYI BstZ17I ClaI CspCI DraI DraIII EcoNI
EcoRI FalI FseI FspAI HpaI KasI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XmnI