Restriction map for
pF1KSDA1479
(Linear) (Six-base) MAPSORT of: KIAA1479.seq Check: 4436 from: 1 to: 3033
KIAA1479.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1840 3033
Size: 1840 1193
AccI GT'mk_AC
Cuts at: 0 98 1862 2140 3033
Size: 98 1764 278 893
Fragments arranged by size:
1764 893 278 98
Acc65I G'GTAC_C
Cuts at: 0 409 3033
Size: 409 2624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 128 1349 1709 2482 3033
Size: 92 36 1221 360 773 551
Fragments arranged by size:
1221 773 551 360 92 36
AflIII A'CryG_T
Cuts at: 0 1877 2327 3033
Size: 1877 450 706
Fragments arranged by size:
1877 706 450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1881 3033
Size: 1881 1152
AlwNI CAG_nnn'CTG
Cuts at: 0 565 1054 1541 3033
Size: 565 489 487 1492
Fragments arranged by size:
1492 565 489 487
ApoI r'AATT_y
Cuts at: 0 191 1711 2712 3033
Size: 191 1520 1001 321
Fragments arranged by size:
1520 1001 321 191
AvaI C'yCGr_G
Cuts at: 0 1244 3033
Size: 1244 1789
AvrII C'CTAG_G
Cuts at: 0 2926 3033
Size: 2926 107
BamHI G'GATC_C
Cuts at: 0 1615 2541 3033
Size: 1615 926 492
Fragments arranged by size:
1615 926 492
BanI G'GyrC_C
Cuts at: 0 409 3033
Size: 409 2624
BanII G_rGCy'C
Cuts at: 0 1229 3033
Size: 1229 1804
BbsI GAAGACnn'nnnn_
Cuts at: 0 1082 1454 1664 2167 3033
Size: 1082 372 210 503 866
Fragments arranged by size:
1082 866 503 372 210
BclI T'GATC_A
Cuts at: 0 2382 3033
Size: 2382 651
BfrBI ATG'CAT
Cuts at: 0 418 3033
Size: 418 2615
BglI GCCn_nnn'nGGC
Cuts at: 0 146 562 3033
Size: 146 416 2471
Fragments arranged by size:
2471 416 146
BmgBI CAC'GTC
Cuts at: 0 2330 2967 3033
Size: 2330 637 66
Fragments arranged by size:
2330 637 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 42 1920 2282 2837 3033
Size: 42 1878 362 555 196
Fragments arranged by size:
1878 555 362 196 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 881 1031 1790 1926 2093 2687 2874
Size: 829 52 150 759 136 167 594 187
Cuts at: 2874 3033
Size: 159
Fragments arranged by size:
829 759 594 187 167 159 150 136
52
BsaI GGTCTCn'nnnn_
Cuts at: 0 1975 3033
Size: 1975 1058
BsaBI GATnn'nnATC
Cuts at: 0 1481 3033
Size: 1481 1552
BsaHI Gr'CG_yC
Cuts at: 0 138 2947 3033
Size: 138 2809 86
Fragments arranged by size:
2809 138 86
BsaWI w'CCGG_w
Cuts at: 0 123 3033
Size: 123 2910
BseYI C'CCAG_C
Cuts at: 0 797 3033
Size: 797 2236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1449 3033
Size: 1449 1584
BsmI GAATG_Cn'
Cuts at: 0 355 418 1840 3033
Size: 355 63 1422 1193
Fragments arranged by size:
1422 1193 355 63
Bsp1286I G_dGCh'C
Cuts at: 0 1229 3033
Size: 1229 1804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1840 2042 3033
Size: 1840 202 991
Fragments arranged by size:
1840 991 202
BsrDI GCAATG_nn'
Cuts at: 0 156 1214 1844 3033
Size: 156 1058 630 1189
Fragments arranged by size:
1189 1058 630 156
BsrFI r'CCGG_y
Cuts at: 0 2420 3033
Size: 2420 613
BssSI C'ACGA_G
Cuts at: 0 1583 3033
Size: 1583 1450
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1846 3033
Size: 1846 1187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1275 1785 2088 2206 2646 3033
Size: 1275 510 303 118 440 387
Fragments arranged by size:
1275 510 440 387 303 118
BstYI r'GATC_y
Cuts at: 0 613 1615 1901 2541 3033
Size: 613 1002 286 640 492
Fragments arranged by size:
1002 640 613 492 286
BstZ17I GTA'TAC
Cuts at: 0 1863 3033
Size: 1863 1170
BtgI C'CryG_G
Cuts at: 0 1743 2081 2199 2205 2546 2802 3033
Size: 1743 338 118 6 341 256 231
Fragments arranged by size:
1743 341 338 256 231 118 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 596 2579 3033
Size: 596 1983 454
Fragments arranged by size:
1983 596 454
BtsI GCAGTG_nn'
Cuts at: 0 1437 3033
Size: 1437 1596
ClaI AT'CG_AT
Cuts at: 0 1148 2554 3033
Size: 1148 1406 479
Fragments arranged by size:
1406 1148 479
DrdI GACnn_nn'nnGTC
Cuts at: 0 1456 1984 3033
Size: 1456 528 1049
Fragments arranged by size:
1456 1049 528
EaeI y'GGCC_r
Cuts at: 0 575 1265 2202 3033
Size: 575 690 937 831
Fragments arranged by size:
937 831 690 575
EarI CTCTTCn'nnn_
Cuts at: 0 1394 2129 2173 2927 3033
Size: 1394 735 44 754 106
Fragments arranged by size:
1394 754 735 106 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1911 2240 3033
Size: 1911 329 793
Fragments arranged by size:
1911 793 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 128 829 881 1031 1349 1709 1790
Size: 92 36 701 52 150 318 360 81
Cuts at: 1790 1926 2093 2482 2687 2874 3033
Size: 136 167 389 205 187 159
Fragments arranged by size:
701 389 360 318 205 187 167 159
150 136 92 81 52 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2721 3033
Size: 2721 312
EcoO109I rG'GnC_Cy
Cuts at: 0 804 2565 3033
Size: 804 1761 468
Fragments arranged by size:
1761 804 468
EcoRI G'AATT_C
Cuts at: 0 1711 3033
Size: 1711 1322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3000 3033
Size: 3000 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1745 1777 1903 1935 2062 2094 2636 2668
Size: 1745 32 126 32 127 32 542 32
Cuts at: 2668 2856 2888 3033
Size: 188 32 145
Fragments arranged by size:
1745 542 188 145 127 126 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 99 1652 3033
Size: 99 1553 1381
Fragments arranged by size:
1553 1381 99
HindIII A'AGCT_T
Cuts at: 0 1130 2368 3033
Size: 1130 1238 665
Fragments arranged by size:
1238 1130 665
HpaI GTT'AAC
Cuts at: 0 1652 3033
Size: 1652 1381
KpnI G_GTAC'C
Cuts at: 0 413 3033
Size: 413 2620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1802 2026 3033
Size: 1454 348 224 1007
Fragments arranged by size:
1454 1007 348 224
MscI TGG'CCA
Cuts at: 0 577 2204 3033
Size: 577 1627 829
Fragments arranged by size:
1627 829 577
MslI CAynn'nnrTG
Cuts at: 0 344 1273 1796 1910 2086 2204 2471 2515
Size: 344 929 523 114 176 118 267 44
Cuts at: 2515 3033
Size: 518
Fragments arranged by size:
929 523 518 344 267 176 118 114
44
MspA1I CmG'CkG
Cuts at: 0 180 801 3033
Size: 180 621 2232
Fragments arranged by size:
2232 621 180
NcoI C'CATG_G
Cuts at: 0 1743 2081 2199 2205 2546 3033
Size: 1743 338 118 6 341 487
Fragments arranged by size:
1743 487 341 338 118 6
NdeI CA'TA_TG
Cuts at: 0 772 3033
Size: 772 2261
NsiI A_TGCA'T
Cuts at: 0 420 3033
Size: 420 2613
NspI r_CATG'y
Cuts at: 0 1881 3033
Size: 1881 1152
PasI CC'CwG_GG
Cuts at: 0 1636 3033
Size: 1636 1397
PciI A'CATG_T
Cuts at: 0 1877 3033
Size: 1877 1156
PflMI CCAn_nnn'nTGG
Cuts at: 0 1099 2065 3033
Size: 1099 966 968
Fragments arranged by size:
1099 968 966
PfoI T'CCnGG_A
Cuts at: 0 806 1154 2664 3033
Size: 806 348 1510 369
Fragments arranged by size:
1510 806 369 348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 3033
Size: 1216 32 1785
Fragments arranged by size:
1785 1216 32
PpuMI rG'GwC_Cy
Cuts at: 0 804 2565 3033
Size: 804 1761 468
Fragments arranged by size:
1761 804 468
PshAI GACnn'nnGTC
Cuts at: 0 1246 3033
Size: 1246 1787
PsiI TTA'TAA
Cuts at: 0 1136 3033
Size: 1136 1897
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1334 1366 3033
Size: 1334 32 1667
Fragments arranged by size:
1667 1334 32
PstI C_TGCA'G
Cuts at: 0 893 2171 2879 3033
Size: 893 1278 708 154
Fragments arranged by size:
1278 893 708 154
PvuII CAG'CTG
Cuts at: 0 180 3033
Size: 180 2853
SalI G'TCGA_C
Cuts at: 0 97 3033
Size: 97 2936
SapI GCTCTTCn'nnn_
Cuts at: 0 2129 2173 3033
Size: 2129 44 860
Fragments arranged by size:
2129 860 44
SbfI CC_TGCA'GG
Cuts at: 0 2171 3033
Size: 2171 862
SfcI C'TryA_G
Cuts at: 0 441 889 2167 2875 3033
Size: 441 448 1278 708 158
Fragments arranged by size:
1278 708 448 441 158
SgrAI Cr'CCGG_yG
Cuts at: 0 2420 3033
Size: 2420 613
SspI AAT'ATT
Cuts at: 0 2007 3033
Size: 2007 1026
StuI AGG'CCT
Cuts at: 0 1098 3033
Size: 1098 1935
StyI C'CwwG_G
Cuts at: 0 1093 1743 2081 2199 2205 2546 2926 3033
Size: 1093 650 338 118 6 341 380 107
Fragments arranged by size:
1093 650 380 341 338 118 107 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 778 1227 2076 3033
Size: 595 183 449 849 957
Fragments arranged by size:
957 849 595 449 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2561 2593 2698 2730 2846 2878 3033
Size: 2561 32 105 32 116 32 155
Fragments arranged by size:
2561 155 116 105 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2949 3033
Size: 2949 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1055 1618 2499 3033
Size: 1055 563 881 534
Fragments arranged by size:
1055 881 563 534
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApoI
AvaI AvrII BamHI BanI BanII BbsI BclI BfrBI
BglI BmgBI BmrI BpmI BsaI BsaBI BsaHI BsaWI
BseYI BsgI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HindIII HpaI KpnI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PsiI PsrI PstI
PvuII SalI SapI SbfI SfcI SgrAI SspI StuI
StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI
BcgI BciVI BglII BlpI Bme1580I BmtI BplI Bpu10I
BpuEI BsaAI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII Bsu36I
CspCI DraI DraIII EagI EcoICRI EcoRV FseI FspI
FspAI HaeII KasI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PmeI PmlI PspOMI PspXI
PvuI RsrII SacI SacII SanDI ScaI SexAI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI TatI XbaI XhoI XmaI XmnI ZraI