Restriction map for
pF1KA1477
(Linear) (Six-base) MAPSORT of: KIAA1477.seq Check: 8545 from: 1 to: 2274
KIAA1477.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1833 2274
Size: 1833 441
AccI GT'mk_AC
Cuts at: 0 300 2274
Size: 300 1974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 817 1120 1174 1673 2274
Size: 662 155 303 54 499 601
Fragments arranged by size:
662 601 499 303 155 54
AflII C'TTAA_G
Cuts at: 0 481 2274
Size: 481 1793
AflIII A'CryG_T
Cuts at: 0 234 2274
Size: 234 2040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 68 100 2274
Size: 68 32 2174
Fragments arranged by size:
2174 68 32
ApoI r'AATT_y
Cuts at: 0 207 418 550 714 1054 1909 2274
Size: 207 211 132 164 340 855 365
Fragments arranged by size:
855 365 340 211 207 164 132
AvaI C'yCGr_G
Cuts at: 0 607 1748 2274
Size: 607 1141 526
Fragments arranged by size:
1141 607 526
BanII G_rGCy'C
Cuts at: 0 1759 2274
Size: 1759 515
BbsI GAAGACnn'nnnn_
Cuts at: 0 2014 2274
Size: 2014 260
BbvCI CC'TCA_GC
Cuts at: 0 1569 2274
Size: 1569 705
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1333 1367 2274
Size: 1333 34 907
Fragments arranged by size:
1333 907 34
BclI T'GATC_A
Cuts at: 0 1203 2274
Size: 1203 1071
BmrI ACTGGGnnnn_n'
Cuts at: 0 1103 2274
Size: 1103 1171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1439 1712 2274
Size: 1439 273 562
Fragments arranged by size:
1439 562 273
Bpu10I CC'TnA_GC
Cuts at: 0 1569 2274
Size: 1569 705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1743 2274
Size: 1743 531
BsaAI yAC'GTr
Cuts at: 0 1476 2274
Size: 1476 798
BsaHI Gr'CG_yC
Cuts at: 0 13 665 2274
Size: 13 652 1609
Fragments arranged by size:
1609 652 13
BsaWI w'CCGG_w
Cuts at: 0 915 1733 2274
Size: 915 818 541
Fragments arranged by size:
915 818 541
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1710 1740 2274
Size: 1710 30 534
Fragments arranged by size:
1710 534 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1300 2274
Size: 1300 974
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2167 2274
Size: 2167 107
BsiHKAI G_wGCw'C
Cuts at: 0 1759 2274
Size: 1759 515
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2117 2274
Size: 2117 157
Bsp1286I G_dGCh'C
Cuts at: 0 1759 2274
Size: 1759 515
BspEI T'CCGG_A
Cuts at: 0 915 2274
Size: 915 1359
BspHI T'CATG_A
Cuts at: 0 876 2274
Size: 876 1398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1833 2274
Size: 1833 441
BsrBI CCG'CTC
Cuts at: 0 36 2274
Size: 36 2238
BsrDI GCAATG_nn'
Cuts at: 0 16 1480 2240 2274
Size: 16 1464 760 34
Fragments arranged by size:
1464 760 34 16
BsrFI r'CCGG_y
Cuts at: 0 1661 2274
Size: 1661 613
BsrGI T'GTAC_A
Cuts at: 0 441 2274
Size: 441 1833
BssSI C'ACGA_G
Cuts at: 0 968 2143 2274
Size: 968 1175 131
Fragments arranged by size:
1175 968 131
BstEII G'GTnAC_C
Cuts at: 0 951 2274
Size: 951 1323
Bsu36I CC'TnA_GG
Cuts at: 0 1253 2274
Size: 1253 1021
BtgI C'CryG_G
Cuts at: 0 384 956 1776 2118 2274
Size: 384 572 820 342 156
Fragments arranged by size:
820 572 384 342 156
BtsI GCAGTG_nn'
Cuts at: 0 1627 2155 2274
Size: 1627 528 119
Fragments arranged by size:
1627 528 119
ClaI AT'CG_AT
Cuts at: 0 858 1470 2274
Size: 858 612 804
Fragments arranged by size:
858 804 612
DraI TTT'AAA
Cuts at: 0 719 2274
Size: 719 1555
DrdI GACnn_nn'nnGTC
Cuts at: 0 949 2274
Size: 949 1325
EaeI y'GGCC_r
Cuts at: 0 708 2274
Size: 708 1566
EarI CTCTTCn'nnn_
Cuts at: 0 875 1139 1551 1952 2274
Size: 875 264 412 401 322
Fragments arranged by size:
875 412 401 322 264
EcoICRI GAG'CTC
Cuts at: 0 1757 2274
Size: 1757 517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 817 1120 1174 1439 1673 1712 2274
Size: 662 155 303 54 265 234 39 562
Fragments arranged by size:
662 562 303 265 234 155 54 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 353 1364 2274
Size: 353 1011 910
Fragments arranged by size:
1011 910 353
EcoO109I rG'GnC_Cy
Cuts at: 0 1100 2274
Size: 1100 1174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 327 359 1452 1484 2274
Size: 101 32 194 32 1093 32 790
Fragments arranged by size:
1093 790 194 101 32 32 32
HincII GTy'rAC
Cuts at: 0 1880 2274
Size: 1880 394
HindIII A'AGCT_T
Cuts at: 0 1655 2274
Size: 1655 619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1311 2274
Size: 1311 963
MscI TGG'CCA
Cuts at: 0 710 2274
Size: 710 1564
MspA1I CmG'CkG
Cuts at: 0 1191 2274
Size: 1191 1083
NcoI C'CATG_G
Cuts at: 0 384 956 1776 2118 2274
Size: 384 572 820 342 156
Fragments arranged by size:
820 572 384 342 156
NdeI CA'TA_TG
Cuts at: 0 1446 2274
Size: 1446 828
NspI r_CATG'y
Cuts at: 0 1864 2274
Size: 1864 410
PfoI T'CCnGG_A
Cuts at: 0 1790 2274
Size: 1790 484
PpuMI rG'GwC_Cy
Cuts at: 0 1100 2274
Size: 1100 1174
PsiI TTA'TAA
Cuts at: 0 1814 2274
Size: 1814 460
PstI C_TGCA'G
Cuts at: 0 1542 2274
Size: 1542 732
SacI G_AGCT'C
Cuts at: 0 1759 2274
Size: 1759 515
SapI GCTCTTCn'nnn_
Cuts at: 0 1551 2274
Size: 1551 723
ScaI AGT'ACT
Cuts at: 0 1719 2274
Size: 1719 555
SfcI C'TryA_G
Cuts at: 0 1538 2274
Size: 1538 736
SmlI C'TyrA_G
Cuts at: 0 481 1758 2274
Size: 481 1277 516
Fragments arranged by size:
1277 516 481
SnaBI TAC'GTA
Cuts at: 0 1476 2274
Size: 1476 798
SpeI A'CTAG_T
Cuts at: 0 2014 2274
Size: 2014 260
SspI AAT'ATT
Cuts at: 0 519 2274
Size: 519 1755
StyI C'CwwG_G
Cuts at: 0 384 618 956 1776 2118 2274
Size: 384 234 338 820 342 156
Fragments arranged by size:
820 384 342 338 234 156
TatI w'GTAC_w
Cuts at: 0 441 461 1717 1924 2274
Size: 441 20 1256 207 350
Fragments arranged by size:
1256 441 350 207 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 2274
Size: 1576 32 666
Fragments arranged by size:
1576 666 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 2019 2274
Size: 222 1797 255
Fragments arranged by size:
1797 255 222
XmnI GAAnn'nnTTC
Cuts at: 0 494 791 2274
Size: 494 297 1483
Fragments arranged by size:
1483 494 297
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AloI ApoI AvaI
BanII BbsI BbvCI BcgI BclI BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstEII Bsu36I BtgI BtsI ClaI DraI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I
HincII HindIII MmeI MscI MspA1I NcoI NdeI NspI
PfoI PpuMI PsiI PstI SacI SapI ScaI SfcI
SmlI SnaBI SpeI SspI StyI TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BsaI BsaBI BseYI BsiEI
BsiWI BsmI BssHII BstAPI BstBI BstXI BstYI BstZ17I
BtgZI CspCI DraIII EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI ZraI