Restriction map for
pF1KA1454
(Linear) (Six-base) MAPSORT of: KIAA1454.seq Check: 8425 from: 1 to: 4197
KIAA1454.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2995 4197
Size: 2995 1202
AccI GT'mk_AC
Cuts at: 0 223 4197
Size: 223 3974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 689 803 1214 1352 1385 1687 2387
Size: 439 250 114 411 138 33 302 700
Cuts at: 2387 2622 2695 3505 4197
Size: 235 73 810 692
Fragments arranged by size:
810 700 692 439 411 302 250 235
138 114 73 33
AfeI AGC'GCT
Cuts at: 0 16 4197
Size: 16 4181
AhdI GACnn_n'nnGTC
Cuts at: 0 4061 4197
Size: 4061 136
AleI CACnn'nnGTG
Cuts at: 0 1001 2920 4197
Size: 1001 1919 1277
Fragments arranged by size:
1919 1277 1001
ApoI r'AATT_y
Cuts at: 0 600 1062 1186 1242 1568 2231 2676 2939
Size: 600 462 124 56 326 663 445 263
Cuts at: 2939 4197
Size: 1258
Fragments arranged by size:
1258 663 600 462 445 326 263 124
56
AvaI C'yCGr_G
Cuts at: 0 2147 4197
Size: 2147 2050
AvrII C'CTAG_G
Cuts at: 0 2802 4197
Size: 2802 1395
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3052 3085 4197
Size: 3052 33 1112
Fragments arranged by size:
3052 1112 33
BamHI G'GATC_C
Cuts at: 0 3495 4197
Size: 3495 702
BanI G'GyrC_C
Cuts at: 0 269 1505 3577 4197
Size: 269 1236 2072 620
Fragments arranged by size:
2072 1236 620 269
BanII G_rGCy'C
Cuts at: 0 297 2034 4197
Size: 297 1737 2163
Fragments arranged by size:
2163 1737 297
BbeI G_GCGC'C
Cuts at: 0 1509 4197
Size: 1509 2688
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 1986 4197
Size: 478 1508 2211
Fragments arranged by size:
2211 1508 478
BbvCI CC'TCA_GC
Cuts at: 0 4145 4197
Size: 4145 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1459 1493 4197
Size: 1459 34 2704
Fragments arranged by size:
2704 1459 34
BclI T'GATC_A
Cuts at: 0 3935 4197
Size: 3935 262
BfrBI ATG'CAT
Cuts at: 0 1578 4197
Size: 1578 2619
BglII A'GATC_T
Cuts at: 0 2727 4197
Size: 2727 1470
BlpI GC'TnA_GC
Cuts at: 0 2303 2308 4197
Size: 2303 5 1889
Fragments arranged by size:
2303 1889 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 717 1023 3364 4197
Size: 312 405 306 2341 833
Fragments arranged by size:
2341 833 405 312 306
BmtI G_CTAG'C
Cuts at: 0 1282 4197
Size: 1282 2915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1541 2543 3560 4021 4043 4197
Size: 251 1290 1002 1017 461 22 154
Fragments arranged by size:
1290 1017 1002 461 251 154 22
Bpu10I CC'TnA_GC
Cuts at: 0 1666 1859 2645 4145 4197
Size: 1666 193 786 1500 52
Fragments arranged by size:
1666 1500 786 193 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2224 4144 4197
Size: 2224 1920 53
Fragments arranged by size:
2224 1920 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 4197
Size: 787 3410
BsaAI yAC'GTr
Cuts at: 0 2002 3312 4197
Size: 2002 1310 885
Fragments arranged by size:
2002 1310 885
BsaBI GATnn'nnATC
Cuts at: 0 3380 4197
Size: 3380 817
BsaHI Gr'CG_yC
Cuts at: 0 534 1506 4197
Size: 534 972 2691
Fragments arranged by size:
2691 972 534
BsaWI w'CCGG_w
Cuts at: 0 1733 4197
Size: 1733 2464
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 623 4197
Size: 70 553 3574
Fragments arranged by size:
3574 553 70
BseYI C'CCAG_C
Cuts at: 0 2910 3592 3759 4197
Size: 2910 682 167 438
Fragments arranged by size:
2910 682 438 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1837 2035 3880 4197
Size: 766 1071 198 1845 317
Fragments arranged by size:
1845 1071 766 317 198
BsiEI CG_ry'CG
Cuts at: 0 3870 4197
Size: 3870 327
BsiHKAI G_wGCw'C
Cuts at: 0 297 2034 4197
Size: 297 1737 2163
Fragments arranged by size:
2163 1737 297
BsmI GAATG_Cn'
Cuts at: 0 3733 4197
Size: 3733 464
Bsp1286I G_dGCh'C
Cuts at: 0 297 2034 4197
Size: 297 1737 2163
Fragments arranged by size:
2163 1737 297
BspEI T'CCGG_A
Cuts at: 0 1733 4197
Size: 1733 2464
BspHI T'CATG_A
Cuts at: 0 27 1911 3798 4197
Size: 27 1884 1887 399
Fragments arranged by size:
1887 1884 399 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1554 2995 4197
Size: 1554 1441 1202
Fragments arranged by size:
1554 1441 1202
BsrBI CCG'CTC
Cuts at: 0 2046 2589 4197
Size: 2046 543 1608
Fragments arranged by size:
2046 1608 543
BsrDI GCAATG_nn'
Cuts at: 0 1313 2394 2967 3437 4197
Size: 1313 1081 573 470 760
Fragments arranged by size:
1313 1081 760 573 470
BsrGI T'GTAC_A
Cuts at: 0 3436 4197
Size: 3436 761
BssSI C'ACGA_G
Cuts at: 0 1493 4197
Size: 1493 2704
BstBI TT'CG_AA
Cuts at: 0 1092 2259 4197
Size: 1092 1167 1938
Fragments arranged by size:
1938 1167 1092
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3639 4197
Size: 3639 558
BstYI r'GATC_y
Cuts at: 0 2727 3495 4197
Size: 2727 768 702
Fragments arranged by size:
2727 768 702
BtgI C'CryG_G
Cuts at: 0 1247 1457 3573 3928 4197
Size: 1247 210 2116 355 269
Fragments arranged by size:
2116 1247 355 269 210
BtsI GCAGTG_nn'
Cuts at: 0 206 842 1434 2319 2380 3018 3596 3765
Size: 206 636 592 885 61 638 578 169
Cuts at: 3765 4197
Size: 432
Fragments arranged by size:
885 638 636 592 578 432 206 169
61
DraIII CAC_nnn'GTG
Cuts at: 0 3271 4197
Size: 3271 926
EaeI y'GGCC_r
Cuts at: 0 3392 3641 3746 3867 4028 4197
Size: 3392 249 105 121 161 169
Fragments arranged by size:
3392 249 169 161 121 105
EagI C'GGCC_G
Cuts at: 0 3867 4197
Size: 3867 330
EarI CTCTTCn'nnn_
Cuts at: 0 1268 1679 1961 2071 2210 2406 2579 3524
Size: 1268 411 282 110 139 196 173 945
Cuts at: 3524 3574 3686 4197
Size: 50 112 511
Fragments arranged by size:
1268 945 511 411 282 196 173 139
112 110 50
EcoICRI GAG'CTC
Cuts at: 0 295 2032 4197
Size: 295 1737 2165
Fragments arranged by size:
2165 1737 295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 439 689 803 1214 1352 1385 1541
Size: 251 188 250 114 411 138 33 156
Cuts at: 1541 1687 2387 2543 2622 2695 3505 3560 4021
Size: 146 700 156 79 73 810 55 461
Cuts at: 4021 4043 4197
Size: 22 154
Fragments arranged by size:
810 700 461 411 251 250 188 156
156 154 146 138 114 79 73 55
33 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3799 4197
Size: 3799 398
EcoO109I rG'GnC_Cy
Cuts at: 0 3297 4197
Size: 3297 900
EcoRI G'AATT_C
Cuts at: 0 1062 1568 2939 4197
Size: 1062 506 1371 1258
Fragments arranged by size:
1371 1258 1062 506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2725 3057 4138 4197
Size: 2725 332 1081 59
Fragments arranged by size:
2725 1081 332 59
HaeII r_GCGC'y
Cuts at: 0 18 1509 4197
Size: 18 1491 2688
Fragments arranged by size:
2688 1491 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 534 566 1906 1938 2746 2778
Size: 465 32 37 32 1340 32 808 32
Cuts at: 2778 3180 3212 3501 3533 3709 3741 4047 4079
Size: 402 32 289 32 176 32 306 32
Cuts at: 4079 4197
Size: 118
Fragments arranged by size:
1340 808 465 402 306 289 176 118
37 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1346 3071 3834 4197
Size: 1346 1725 763 363
Fragments arranged by size:
1725 1346 763 363
HindIII A'AGCT_T
Cuts at: 0 594 1699 2567 2707 4197
Size: 594 1105 868 140 1490
Fragments arranged by size:
1490 1105 868 594 140
HpaI GTT'AAC
Cuts at: 0 1346 4197
Size: 1346 2851
KasI G'GCGC_C
Cuts at: 0 1505 4197
Size: 1505 2692
MfeI C'AATT_G
Cuts at: 0 1613 1829 4197
Size: 1613 216 2368
Fragments arranged by size:
2368 1613 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 4197
Size: 927 3270
MscI TGG'CCA
Cuts at: 0 3394 3643 3748 4030 4197
Size: 3394 249 105 282 167
Fragments arranged by size:
3394 282 249 167 105
MslI CAynn'nnrTG
Cuts at: 0 504 1001 1577 2920 3637 4197
Size: 504 497 576 1343 717 560
Fragments arranged by size:
1343 717 576 560 504 497
MspA1I CmG'CkG
Cuts at: 0 2194 3514 4197
Size: 2194 1320 683
Fragments arranged by size:
2194 1320 683
NarI GG'CG_CC
Cuts at: 0 1506 4197
Size: 1506 2691
NcoI C'CATG_G
Cuts at: 0 1247 1457 3573 4197
Size: 1247 210 2116 624
Fragments arranged by size:
2116 1247 624 210
NdeI CA'TA_TG
Cuts at: 0 3551 4197
Size: 3551 646
NheI G'CTAG_C
Cuts at: 0 1278 4197
Size: 1278 2919
NsiI A_TGCA'T
Cuts at: 0 1580 4197
Size: 1580 2617
NspI r_CATG'y
Cuts at: 0 1578 2326 3412 3430 4197
Size: 1578 748 1086 18 767
Fragments arranged by size:
1578 1086 767 748 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 1463 1564 4197
Size: 1463 101 2633
Fragments arranged by size:
2633 1463 101
PfoI T'CCnGG_A
Cuts at: 0 528 4197
Size: 528 3669
PmlI CAC'GTG
Cuts at: 0 2002 4197
Size: 2002 2195
PpuMI rG'GwC_Cy
Cuts at: 0 3297 4197
Size: 3297 900
PsiI TTA'TAA
Cuts at: 0 1882 2699 4197
Size: 1882 817 1498
Fragments arranged by size:
1882 1498 817
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 948 980 4197
Size: 948 32 3217
Fragments arranged by size:
3217 948 32
PstI C_TGCA'G
Cuts at: 0 201 3013 3407 3772 3890 4197
Size: 201 2812 394 365 118 307
Fragments arranged by size:
2812 394 365 307 201 118
PvuII CAG'CTG
Cuts at: 0 2194 3514 4197
Size: 2194 1320 683
Fragments arranged by size:
2194 1320 683
SacI G_AGCT'C
Cuts at: 0 297 2034 4197
Size: 297 1737 2163
Fragments arranged by size:
2163 1737 297
SapI GCTCTTCn'nnn_
Cuts at: 0 1268 1961 2579 3524 3686 4197
Size: 1268 693 618 945 162 511
Fragments arranged by size:
1268 945 693 618 511 162
ScaI AGT'ACT
Cuts at: 0 195 3944 4197
Size: 195 3749 253
Fragments arranged by size:
3749 253 195
SexAI A'CCwGG_T
Cuts at: 0 3848 4197
Size: 3848 349
SfcI C'TryA_G
Cuts at: 0 197 968 1960 3009 3403 3768 3886 4197
Size: 197 771 992 1049 394 365 118 311
Fragments arranged by size:
1049 992 771 394 365 311 197 118
SfoI GGC'GCC
Cuts at: 0 1507 4197
Size: 1507 2690
SmaI CCC'GGG
Cuts at: 0 2149 4197
Size: 2149 2048
SmlI C'TyrA_G
Cuts at: 0 2239 4123 4197
Size: 2239 1884 74
Fragments arranged by size:
2239 1884 74
SphI G_CATG'C
Cuts at: 0 3412 4197
Size: 3412 785
StuI AGG'CCT
Cuts at: 0 754 813 4153 4197
Size: 754 59 3340 44
Fragments arranged by size:
3340 754 59 44
StyI C'CwwG_G
Cuts at: 0 1247 1457 2802 3573 3608 4104 4197
Size: 1247 210 1345 771 35 496 93
Fragments arranged by size:
1345 1247 771 496 210 93 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3854 4197
Size: 3854 343
TatI w'GTAC_w
Cuts at: 0 193 1042 2525 2551 2742 3436 3942 4197
Size: 193 849 1483 26 191 694 506 255
Fragments arranged by size:
1483 849 694 506 255 193 191 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 524 556 3588 3620 4197
Size: 524 32 3032 32 577
Fragments arranged by size:
3032 577 524 32 32
XbaI T'CTAG_A
Cuts at: 0 1290 2034 4197
Size: 1290 744 2163
Fragments arranged by size:
2163 1290 744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3756 4197
Size: 3756 441
XmaI C'CCGG_G
Cuts at: 0 2147 4197
Size: 2147 2050
XmnI GAAnn'nnTTC
Cuts at: 0 1187 2255 4197
Size: 1187 1068 1942
Fragments arranged by size:
1942 1187 1068
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI ApoI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BfrBI BglII BlpI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstBI BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NsiI NspI PflMI PfoI PmlI
PpuMI PsiI PsrI PstI PvuII SacI SapI ScaI
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI BciVI BglI
Bme1580I BmgBI BplI BsaXI BsiWI BsmBI BsrFI BssHII
BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoRV FseI FspI FspAI KpnI MluI
NaeI NgoMIV NotI NruI PacI PasI PciI PmeI
PpiI PshAI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XhoI ZraI