Restriction map for
pF1KA1452
(Linear) (Six-base) MAPSORT of: KIAA1452.seq Check: 9933 from: 1 to: 2124
KIAA1452.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 629 2124
Size: 629 1495
Acc65I G'GTAC_C
Cuts at: 0 2099 2124
Size: 2099 25
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1705 1779 2124
Size: 1705 74 345
Fragments arranged by size:
1705 345 74
AflIII A'CryG_T
Cuts at: 0 1415 2124
Size: 1415 709
AleI CACnn'nnGTG
Cuts at: 0 1669 2124
Size: 1669 455
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1351 1857 2124
Size: 672 679 506 267
Fragments arranged by size:
679 672 506 267
ApaI G_GGCC'C
Cuts at: 0 907 2124
Size: 907 1217
ApoI r'AATT_y
Cuts at: 0 1964 2124
Size: 1964 160
AvaI C'yCGr_G
Cuts at: 0 1606 1676 2124
Size: 1606 70 448
Fragments arranged by size:
1606 448 70
BamHI G'GATC_C
Cuts at: 0 1379 2124
Size: 1379 745
BanI G'GyrC_C
Cuts at: 0 1461 1640 1860 2099 2124
Size: 1461 179 220 239 25
Fragments arranged by size:
1461 239 220 179 25
BanII G_rGCy'C
Cuts at: 0 371 611 713 907 1502 1553 2124
Size: 371 240 102 194 595 51 571
Fragments arranged by size:
595 571 371 240 194 102 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 2124
Size: 506 1618
BbvCI CC'TCA_GC
Cuts at: 0 1037 2124
Size: 1037 1087
BciVI GTATCCnnnnn_n'
Cuts at: 0 1285 2124
Size: 1285 839
BclI T'GATC_A
Cuts at: 0 1938 2124
Size: 1938 186
BglI GCCn_nnn'nGGC
Cuts at: 0 469 902 1356 1608 2124
Size: 469 433 454 252 516
Fragments arranged by size:
516 469 454 433 252
BglII A'GATC_T
Cuts at: 0 2034 2124
Size: 2034 90
BlpI GC'TnA_GC
Cuts at: 0 1732 2124
Size: 1732 392
Bme1580I G_kGCm'C
Cuts at: 0 682 907 1624 1643 2124
Size: 682 225 717 19 481
Fragments arranged by size:
717 682 481 225 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 16 719 981 2124
Size: 16 703 262 1143
Fragments arranged by size:
1143 703 262 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2053 2124
Size: 2053 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1444 1946 2124
Size: 1177 267 502 178
Fragments arranged by size:
1177 502 267 178
Bpu10I CC'TnA_GC
Cuts at: 0 1037 2124
Size: 1037 1087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 672 1726 2003 2124
Size: 193 479 1054 277 121
Fragments arranged by size:
1054 479 277 193 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 256 2124
Size: 256 1868
BsaAI yAC'GTr
Cuts at: 0 272 2124
Size: 272 1852
BsaBI GATnn'nnATC
Cuts at: 0 181 847 1943 2124
Size: 181 666 1096 181
Fragments arranged by size:
1096 666 181 181
BsaHI Gr'CG_yC
Cuts at: 0 437 2124
Size: 437 1687
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 778 781 1510 1525 1534 1543 1558
Size: 619 159 3 729 15 9 9 15
Cuts at: 1558 1561 2124
Size: 3 563
Fragments arranged by size:
729 619 563 159 15 15 9 9
3 3
BseYI C'CCAG_C
Cuts at: 0 409 754 790 812 1091 1190 1403 1567
Size: 409 345 36 22 279 99 213 164
Cuts at: 1567 2124
Size: 557
Fragments arranged by size:
557 409 345 279 213 164 99 36
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 589 1744 2124
Size: 332 257 1155 380
Fragments arranged by size:
1155 380 332 257
BsiEI CG_ry'CG
Cuts at: 0 538 2124
Size: 538 1586
BsiHKAI G_wGCw'C
Cuts at: 0 371 713 1046 1163 1727 2124
Size: 371 342 333 117 564 397
Fragments arranged by size:
564 397 371 342 333 117
Bsp1286I G_dGCh'C
Cuts at: 0 371 611 682 713 907 1046 1163 1502
Size: 371 240 71 31 194 139 117 339
Cuts at: 1502 1553 1624 1643 1727 2124
Size: 51 71 19 84 397
Fragments arranged by size:
397 371 339 240 194 139 117 84
71 71 51 31 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 629 680 2124
Size: 629 51 1444
Fragments arranged by size:
1444 629 51
BsrBI CCG'CTC
Cuts at: 0 1428 2124
Size: 1428 696
BsrFI r'CCGG_y
Cuts at: 0 1840 2124
Size: 1840 284
BsrGI T'GTAC_A
Cuts at: 0 27 2124
Size: 27 2097
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1260 2124
Size: 1260 864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 2124
Size: 60 2064
BstYI r'GATC_y
Cuts at: 0 848 1379 2034 2124
Size: 848 531 655 90
Fragments arranged by size:
848 655 531 90
Bsu36I CC'TnA_GG
Cuts at: 0 637 2124
Size: 637 1487
BtgI C'CryG_G
Cuts at: 0 920 1169 1553 2124
Size: 920 249 384 571
Fragments arranged by size:
920 571 384 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 967 2124
Size: 967 1157
ClaI AT'CG_AT
Cuts at: 0 38 914 2124
Size: 38 876 1210
Fragments arranged by size:
1210 876 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 677 712 2124
Size: 677 35 1412
Fragments arranged by size:
1412 677 35
DraIII CAC_nnn'GTG
Cuts at: 0 727 2124
Size: 727 1397
DrdI GACnn_nn'nnGTC
Cuts at: 0 2118 2124
Size: 2118 6
EaeI y'GGCC_r
Cuts at: 0 2 50 179 461 535 833 1166 1589
Size: 2 48 129 282 74 298 333 423
Cuts at: 1589 1766 1782 1835 2124
Size: 177 16 53 289
Fragments arranged by size:
423 333 298 289 282 177 129 74
53 48 16 2
EagI C'GGCC_G
Cuts at: 0 535 2124
Size: 535 1589
EarI CTCTTCn'nnn_
Cuts at: 0 5 315 486 976 1756 1798 2124
Size: 5 310 171 490 780 42 326
Fragments arranged by size:
780 490 326 310 171 42 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 511 1458 1555 2124
Size: 79 432 947 97 569
Fragments arranged by size:
947 569 432 97 79
EcoICRI GAG'CTC
Cuts at: 0 369 711 2124
Size: 369 342 1413
Fragments arranged by size:
1413 369 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1444 1705 1779 1946 2124
Size: 1177 267 261 74 167 178
Fragments arranged by size:
1177 267 261 178 167 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 2124
Size: 427 1697
EcoO109I rG'GnC_Cy
Cuts at: 0 248 904 1348 2124
Size: 248 656 444 776
Fragments arranged by size:
776 656 444 248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 318 1767 2124
Size: 318 1449 357
Fragments arranged by size:
1449 357 318
FspI TGC'GCA
Cuts at: 0 820 2124
Size: 820 1304
HaeII r_GCGC'y
Cuts at: 0 564 1749 2124
Size: 564 1185 375
Fragments arranged by size:
1185 564 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 630 634 662 666 1327 1359 1992 2024
Size: 630 4 28 4 661 32 633 32
Cuts at: 2024 2124
Size: 100
Fragments arranged by size:
661 633 630 100 32 32 28 4
4
HincII GTy'rAC
Cuts at: 0 2015 2124
Size: 2015 109
KpnI G_GTAC'C
Cuts at: 0 2103 2124
Size: 2103 21
MluI A'CGCG_T
Cuts at: 0 1415 2124
Size: 1415 709
MscI TGG'CCA
Cuts at: 0 4 52 181 1591 1768 2124
Size: 4 48 129 1410 177 356
Fragments arranged by size:
1410 356 177 129 48 4
MslI CAynn'nnrTG
Cuts at: 0 629 1168 1238 1669 2124
Size: 629 539 70 431 455
Fragments arranged by size:
629 539 455 431 70
MspA1I CmG'CkG
Cuts at: 0 346 402 816 1025 1091 1251 1377 1440
Size: 346 56 414 209 66 160 126 63
Cuts at: 1440 1455 1571 1799 2124
Size: 15 116 228 325
Fragments arranged by size:
414 346 325 228 209 160 126 116
66 63 56 15
NcoI C'CATG_G
Cuts at: 0 1553 2124
Size: 1553 571
NspI r_CATG'y
Cuts at: 0 1263 1818 2097 2124
Size: 1263 555 279 27
Fragments arranged by size:
1263 555 279 27
PasI CC'CwG_GG
Cuts at: 0 611 1650 2124
Size: 611 1039 474
Fragments arranged by size:
1039 611 474
PflMI CCAn_nnn'nTGG
Cuts at: 0 948 2124
Size: 948 1176
PfoI T'CCnGG_A
Cuts at: 0 1154 2124
Size: 1154 970
PspOMI G'GGCC_C
Cuts at: 0 903 2124
Size: 903 1221
PstI C_TGCA'G
Cuts at: 0 401 590 1051 1439 2124
Size: 401 189 461 388 685
Fragments arranged by size:
685 461 401 388 189
PvuII CAG'CTG
Cuts at: 0 402 816 1455 2124
Size: 402 414 639 669
Fragments arranged by size:
669 639 414 402
RsrII CG'GwC_CG
Cuts at: 0 1809 2124
Size: 1809 315
SacI G_AGCT'C
Cuts at: 0 371 713 2124
Size: 371 342 1411
Fragments arranged by size:
1411 371 342
SapI GCTCTTCn'nnn_
Cuts at: 0 315 2124
Size: 315 1809
ScaI AGT'ACT
Cuts at: 0 2063 2124
Size: 2063 61
SfcI C'TryA_G
Cuts at: 0 354 397 586 1047 1435 1819 2124
Size: 354 43 189 461 388 384 305
Fragments arranged by size:
461 388 384 354 305 189 43
SmlI C'TyrA_G
Cuts at: 0 172 687 1741 2018 2124
Size: 172 515 1054 277 106
Fragments arranged by size:
1054 515 277 172 106
SphI G_CATG'C
Cuts at: 0 1818 2124
Size: 1818 306
StuI AGG'CCT
Cuts at: 0 1690 2124
Size: 1690 434
StyI C'CwwG_G
Cuts at: 0 963 1001 1397 1553 2124
Size: 963 38 396 156 571
Fragments arranged by size:
963 571 396 156 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 768 963 1083 2124
Size: 738 30 195 120 1041
Fragments arranged by size:
1041 738 195 120 30
TatI w'GTAC_w
Cuts at: 0 27 44 2061 2115 2124
Size: 27 17 2017 54 9
Fragments arranged by size:
2017 54 27 17 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 918 1668 2124
Size: 918 750 456
Fragments arranged by size:
918 750 456
XmnI GAAnn'nnTTC
Cuts at: 0 1150 1690 2124
Size: 1150 540 434
Fragments arranged by size:
1150 540 434
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII KpnI MluI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PspOMI PstI PvuII RsrII SacI SapI ScaI SfcI
SmlI SphI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BfrBI BmgBI BmtI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI BstEII
BstZ17I BtsI DraI EcoRI EcoRV FseI FspAI HindIII
HpaI KasI MfeI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI