Restriction map for
pF1KA1450
(Linear) (Six-base) MAPSORT of: KIAA1450.seq Check: 2957 from: 1 to: 3342
KIAA1450.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1060 2625 2669 2702 2929 2996 3088
Size: 905 155 1565 44 33 227 67 92
Cuts at: 3088 3342
Size: 254
Fragments arranged by size:
1565 905 254 227 155 92 67 44
33
AfeI AGC'GCT
Cuts at: 0 1246 3342
Size: 1246 2096
AflIII A'CryG_T
Cuts at: 0 407 3342
Size: 407 2935
AgeI A'CCGG_T
Cuts at: 0 2087 3342
Size: 2087 1255
AleI CACnn'nnGTG
Cuts at: 0 1513 2744 3342
Size: 1513 1231 598
Fragments arranged by size:
1513 1231 598
AlwNI CAG_nnn'CTG
Cuts at: 0 536 2185 2919 3342
Size: 536 1649 734 423
Fragments arranged by size:
1649 734 536 423
ApaI G_GGCC'C
Cuts at: 0 2584 3342
Size: 2584 758
ApoI r'AATT_y
Cuts at: 0 981 1141 3342
Size: 981 160 2201
Fragments arranged by size:
2201 981 160
BanI G'GyrC_C
Cuts at: 0 1895 1968 3342
Size: 1895 73 1374
Fragments arranged by size:
1895 1374 73
BanII G_rGCy'C
Cuts at: 0 84 1727 2299 2584 3342
Size: 84 1643 572 285 758
Fragments arranged by size:
1643 758 572 285 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 704 3342
Size: 504 200 2638
Fragments arranged by size:
2638 504 200
BclI T'GATC_A
Cuts at: 0 746 3342
Size: 746 2596
BfrBI ATG'CAT
Cuts at: 0 1939 3159 3342
Size: 1939 1220 183
Fragments arranged by size:
1939 1220 183
BglI GCCn_nnn'nGGC
Cuts at: 0 1808 2257 3342
Size: 1808 449 1085
Fragments arranged by size:
1808 1085 449
BglII A'GATC_T
Cuts at: 0 2730 3342
Size: 2730 612
BlpI GC'TnA_GC
Cuts at: 0 2437 3342
Size: 2437 905
Bme1580I G_kGCm'C
Cuts at: 0 1458 1804 1813 2461 2584 3342
Size: 1458 346 9 648 123 758
Fragments arranged by size:
1458 758 648 346 123 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1401 3271 3342
Size: 1401 1870 71
Fragments arranged by size:
1870 1401 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1520 3342
Size: 1520 1822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 1475 1629 2162 2521 2569 2777 3342
Size: 1 1474 154 533 359 48 208 565
Fragments arranged by size:
1474 565 533 359 208 154 48 1
Bpu10I CC'TnA_GC
Cuts at: 0 356 2273 3342
Size: 356 1917 1069
Fragments arranged by size:
1917 1069 356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 3342
Size: 1237 2105
BsaI GGTCTCn'nnnn_
Cuts at: 0 1817 2798 3084 3342
Size: 1817 981 286 258
Fragments arranged by size:
1817 981 286 258
BsaHI Gr'CG_yC
Cuts at: 0 64 3342
Size: 64 3278
BsaWI w'CCGG_w
Cuts at: 0 2087 3342
Size: 2087 1255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 1681 1711 3300 3330 3342
Size: 439 30 1212 30 1589 30 12
Fragments arranged by size:
1589 1212 439 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 476 762 1702 2508 3342
Size: 232 244 286 940 806 834
Fragments arranged by size:
940 834 806 286 244 232
BseYI C'CCAG_C
Cuts at: 0 1461 1996 2428 3342
Size: 1461 535 432 914
Fragments arranged by size:
1461 914 535 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2495 3037 3342
Size: 2495 542 305
Fragments arranged by size:
2495 542 305
BsmI GAATG_Cn'
Cuts at: 0 1941 3342
Size: 1941 1401
Bsp1286I G_dGCh'C
Cuts at: 0 84 1458 1727 1804 1813 2299 2461 2584
Size: 84 1374 269 77 9 486 162 123
Cuts at: 2584 3342
Size: 758
Fragments arranged by size:
1374 758 486 269 162 123 84 77
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2274 3009 3290 3342
Size: 2274 735 281 52
Fragments arranged by size:
2274 735 281 52
BsrBI CCG'CTC
Cuts at: 0 1729 2834 3342
Size: 1729 1105 508
Fragments arranged by size:
1729 1105 508
BsrDI GCAATG_nn'
Cuts at: 0 713 3342
Size: 713 2629
BsrFI r'CCGG_y
Cuts at: 0 2087 2890 3342
Size: 2087 803 452
Fragments arranged by size:
2087 803 452
BssSI C'ACGA_G
Cuts at: 0 2590 3230 3342
Size: 2590 640 112
Fragments arranged by size:
2590 640 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2425 3342
Size: 2425 917
BstBI TT'CG_AA
Cuts at: 0 813 3342
Size: 813 2529
BstEII G'GTnAC_C
Cuts at: 0 1630 3342
Size: 1630 1712
BstYI r'GATC_y
Cuts at: 0 340 585 2730 3342
Size: 340 245 2145 612
Fragments arranged by size:
2145 612 340 245
Bsu36I CC'TnA_GG
Cuts at: 0 86 3342
Size: 86 3256
BtgI C'CryG_G
Cuts at: 0 56 2469 3342
Size: 56 2413 873
Fragments arranged by size:
2413 873 56
BtsI GCAGTG_nn'
Cuts at: 0 294 2901 2920 3342
Size: 294 2607 19 422
Fragments arranged by size:
2607 422 294 19
DraI TTT'AAA
Cuts at: 0 1643 2304 3342
Size: 1643 661 1038
Fragments arranged by size:
1643 1038 661
DrdI GACnn_nn'nnGTC
Cuts at: 0 2760 3342
Size: 2760 582
EaeI y'GGCC_r
Cuts at: 0 1424 1780 2829 2924 3342
Size: 1424 356 1049 95 418
Fragments arranged by size:
1424 1049 418 356 95
EarI CTCTTCn'nnn_
Cuts at: 0 31 324 1036 1989 3342
Size: 31 293 712 953 1353
Fragments arranged by size:
1353 953 712 293 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 905 1060 1475 1629 2162 2521 2569
Size: 1 904 155 415 154 533 359 48
Cuts at: 2569 2625 2669 2702 2777 2929 2996 3088 3342
Size: 56 44 33 75 152 67 92 254
Fragments arranged by size:
904 533 415 359 254 155 154 152
92 75 67 56 48 44 33 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 89 3342
Size: 89 3253
EcoO109I rG'GnC_Cy
Cuts at: 0 94 628 1344 2277 2539 2580 2581 2762
Size: 94 534 716 933 262 41 1 181
Cuts at: 2762 3342
Size: 580
Fragments arranged by size:
933 716 580 534 262 181 94 41
1
EcoRI G'AATT_C
Cuts at: 0 1141 3342
Size: 1141 2201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 410 2655 3342
Size: 410 2245 687
Fragments arranged by size:
2245 687 410
HaeII r_GCGC'y
Cuts at: 0 804 1248 2687 3342
Size: 804 444 1439 655
Fragments arranged by size:
1439 804 655 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1139 1148 1171 1602 1634 3342
Size: 1116 23 9 23 431 32 1708
Fragments arranged by size:
1708 1116 431 32 23 23 9
HincII GTy'rAC
Cuts at: 0 405 860 1325 3293 3342
Size: 405 455 465 1968 49
Fragments arranged by size:
1968 465 455 405 49
HindIII A'AGCT_T
Cuts at: 0 2166 3127 3342
Size: 2166 961 215
Fragments arranged by size:
2166 961 215
HpaI GTT'AAC
Cuts at: 0 1325 3342
Size: 1325 2017
MfeI C'AATT_G
Cuts at: 0 1049 3342
Size: 1049 2293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3299 3342
Size: 3299 43
MscI TGG'CCA
Cuts at: 0 1426 3342
Size: 1426 1916
MslI CAynn'nnrTG
Cuts at: 0 1360 1513 1711 2744 2863 3342
Size: 1360 153 198 1033 119 479
Fragments arranged by size:
1360 1033 479 198 153 119
MspA1I CmG'CkG
Cuts at: 0 47 58 806 1461 1602 2000 2577 3342
Size: 47 11 748 655 141 398 577 765
Fragments arranged by size:
765 748 655 577 398 141 47 11
NsiI A_TGCA'T
Cuts at: 0 1941 3161 3342
Size: 1941 1220 181
Fragments arranged by size:
1941 1220 181
NspI r_CATG'y
Cuts at: 0 411 1422 2868 3342
Size: 411 1011 1446 474
Fragments arranged by size:
1446 1011 474 411
PasI CC'CwG_GG
Cuts at: 0 72 2688 3342
Size: 72 2616 654
Fragments arranged by size:
2616 654 72
PciI A'CATG_T
Cuts at: 0 407 3342
Size: 407 2935
PfoI T'CCnGG_A
Cuts at: 0 986 1921 2123 2957 3342
Size: 986 935 202 834 385
Fragments arranged by size:
986 935 834 385 202
PpuMI rG'GwC_Cy
Cuts at: 0 94 628 1344 2277 2539 2762 3342
Size: 94 534 716 933 262 223 580
Fragments arranged by size:
933 716 580 534 262 223 94
PshAI GACnn'nnGTC
Cuts at: 0 1829 3342
Size: 1829 1513
PspOMI G'GGCC_C
Cuts at: 0 2580 3342
Size: 2580 762
PvuII CAG'CTG
Cuts at: 0 1461 1602 2000 3342
Size: 1461 141 398 1342
Fragments arranged by size:
1461 1342 398 141
SacII CC_GC'GG
Cuts at: 0 59 3342
Size: 59 3283
SanDI GG'GwC_CC
Cuts at: 0 1344 3342
Size: 1344 1998
SapI GCTCTTCn'nnn_
Cuts at: 0 31 1989 3342
Size: 31 1958 1353
Fragments arranged by size:
1958 1353 31
ScaI AGT'ACT
Cuts at: 0 3309 3342
Size: 3309 33
SfcI C'TryA_G
Cuts at: 0 894 1071 3175 3342
Size: 894 177 2104 167
Fragments arranged by size:
2104 894 177 167
SmlI C'TyrA_G
Cuts at: 0 1252 3342
Size: 1252 2090
StuI AGG'CCT
Cuts at: 0 2230 3342
Size: 2230 1112
StyI C'CwwG_G
Cuts at: 0 1177 1667 2243 2765 2788 3342
Size: 1177 490 576 522 23 554
Fragments arranged by size:
1177 576 554 522 490 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1453 2824 3342
Size: 1453 1371 518
Fragments arranged by size:
1453 1371 518
TatI w'GTAC_w
Cuts at: 0 380 544 572 3307 3342
Size: 380 164 28 2735 35
Fragments arranged by size:
2735 380 164 35 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2880 2912 3342
Size: 2880 32 430
Fragments arranged by size:
2880 430 32
Tth111I GACn'n_nGTC
Cuts at: 0 1537 2932 3342
Size: 1537 1395 410
Fragments arranged by size:
1537 1395 410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 331 1642 2581 2730 3342
Size: 331 1311 939 149 612
Fragments arranged by size:
1311 939 612 331 149
XmnI GAAnn'nnTTC
Cuts at: 0 25 365 3342
Size: 25 340 2977
Fragments arranged by size:
2977 340 25
Enzymes that do cut:
AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApoI
BanI BanII BbsI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtsI DraI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NsiI NspI
PasI PciI PfoI PpuMI PshAI PspOMI PvuII SacII
SanDI SapI ScaI SfcI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BmgBI BmtI BsaAI
BsaBI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrGI
BssHII BstXI BstZ17I BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoRV FseI FspI FspAI KasI KpnI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PstI PvuI RsrII SacI SalI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmaI ZraI