Restriction map for
pF1KSDA1443
(Linear) (Six-base) MAPSORT of: KIAA1443.seq Check: 4537 from: 1 to: 1647
KIAA1443.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 215 1470 1647
Size: 215 1255 177
Fragments arranged by size:
1255 215 177
AccI GT'mk_AC
Cuts at: 0 380 1647
Size: 380 1267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 204 368 1647
Size: 68 136 164 1279
Fragments arranged by size:
1279 164 136 68
AlwNI CAG_nnn'CTG
Cuts at: 0 882 1273 1485 1647
Size: 882 391 212 162
Fragments arranged by size:
882 391 212 162
AseI AT'TA_AT
Cuts at: 0 802 1647
Size: 802 845
AvaI C'yCGr_G
Cuts at: 0 25 365 371 776 1647
Size: 25 340 6 405 871
Fragments arranged by size:
871 405 340 25 6
BanI G'GyrC_C
Cuts at: 0 67 453 1276 1647
Size: 67 386 823 371
Fragments arranged by size:
823 386 371 67
BanII G_rGCy'C
Cuts at: 0 122 372 563 1647
Size: 122 250 191 1084
Fragments arranged by size:
1084 250 191 122
BbvCI CC'TCA_GC
Cuts at: 0 1511 1520 1647
Size: 1511 9 127
Fragments arranged by size:
1511 127 9
BclI T'GATC_A
Cuts at: 0 1634 1647
Size: 1634 13
BglI GCCn_nnn'nGGC
Cuts at: 0 27 999 1647
Size: 27 972 648
Fragments arranged by size:
972 648 27
BlpI GC'TnA_GC
Cuts at: 0 1468 1647
Size: 1468 179
Bme1580I G_kGCm'C
Cuts at: 0 56 1143 1281 1647
Size: 56 1087 138 366
Fragments arranged by size:
1087 366 138 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 947 1416 1647
Size: 947 469 231
Fragments arranged by size:
947 469 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 579 1647
Size: 579 1068
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 461 901 1647
Size: 449 12 440 746
Fragments arranged by size:
746 449 440 12
Bpu10I CC'TnA_GC
Cuts at: 0 1511 1520 1647
Size: 1511 9 127
Fragments arranged by size:
1511 127 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 1647
Size: 103 1544
BsaWI w'CCGG_w
Cuts at: 0 1380 1647
Size: 1380 267
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 1647
Size: 60 30 1557
Fragments arranged by size:
1557 60 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 449 452 463 571 838 1570 1573
Size: 160 289 3 11 108 267 732 3
Cuts at: 1573 1606 1609 1612 1615 1647
Size: 33 3 3 3 32
Fragments arranged by size:
732 289 267 160 108 33 32 11
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 11 238 314 764 1296 1475 1647
Size: 11 227 76 450 532 179 172
Fragments arranged by size:
532 450 227 179 172 76 11
BsmI GAATG_Cn'
Cuts at: 0 634 1647
Size: 634 1013
Bsp1286I G_dGCh'C
Cuts at: 0 56 122 372 563 1143 1281 1647
Size: 56 66 250 191 580 138 366
Fragments arranged by size:
580 366 250 191 138 66 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 1470 1647
Size: 215 1255 177
Fragments arranged by size:
1255 215 177
BsrBI CCG'CTC
Cuts at: 0 1328 1647
Size: 1328 319
BsrDI GCAATG_nn'
Cuts at: 0 204 251 1647
Size: 204 47 1396
Fragments arranged by size:
1396 204 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 253 805 1647
Size: 253 552 842
Fragments arranged by size:
842 552 253
BstXI CCAn_nnnn'nTGG
Cuts at: 0 721 1647
Size: 721 926
Bsu36I CC'TnA_GG
Cuts at: 0 554 1647
Size: 554 1093
BtsI GCAGTG_nn'
Cuts at: 0 577 633 681 1647
Size: 577 56 48 966
Fragments arranged by size:
966 577 56 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1231 1266 1647
Size: 1231 35 381
Fragments arranged by size:
1231 381 35
DraIII CAC_nnn'GTG
Cuts at: 0 955 1147 1647
Size: 955 192 500
Fragments arranged by size:
955 500 192
EarI CTCTTCn'nnn_
Cuts at: 0 353 855 870 1541 1547 1583 1647
Size: 353 502 15 671 6 36 64
Fragments arranged by size:
671 502 353 64 36 15 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 204 368 449 461 901 1647
Size: 68 136 164 81 12 440 746
Fragments arranged by size:
746 440 164 136 81 68 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 685 1398 1647
Size: 685 713 249
Fragments arranged by size:
713 685 249
EcoO109I rG'GnC_Cy
Cuts at: 0 502 706 1647
Size: 502 204 941
Fragments arranged by size:
941 502 204
EcoRV GAT'ATC
Cuts at: 0 1440 1647
Size: 1440 207
HaeII r_GCGC'y
Cuts at: 0 321 1647
Size: 321 1326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1341 1373 1647
Size: 817 32 492 32 274
Fragments arranged by size:
817 492 274 32 32
MfeI C'AATT_G
Cuts at: 0 794 1447 1647
Size: 794 653 200
Fragments arranged by size:
794 653 200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1647
Size: 532 1115
MspA1I CmG'CkG
Cuts at: 0 95 115 326 1101 1519 1647
Size: 95 20 211 775 418 128
Fragments arranged by size:
775 418 211 128 95 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 882 1647
Size: 882 765
PfoI T'CCnGG_A
Cuts at: 0 739 1647
Size: 739 908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 1647
Size: 194 32 1421
Fragments arranged by size:
1421 194 32
PpuMI rG'GwC_Cy
Cuts at: 0 502 706 1647
Size: 502 204 941
Fragments arranged by size:
941 502 204
PspXI vC'TCGA_Gb
Cuts at: 0 365 1647
Size: 365 1282
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 950 982 1647
Size: 950 32 665
Fragments arranged by size:
950 665 32
PvuII CAG'CTG
Cuts at: 0 1101 1519 1647
Size: 1101 418 128
Fragments arranged by size:
1101 418 128
SanDI GG'GwC_CC
Cuts at: 0 706 1647
Size: 706 941
SexAI A'CCwGG_T
Cuts at: 0 1020 1272 1647
Size: 1020 252 375
Fragments arranged by size:
1020 375 252
SfcI C'TryA_G
Cuts at: 0 151 1647
Size: 151 1496
SmaI CCC'GGG
Cuts at: 0 27 1647
Size: 27 1620
SmlI C'TyrA_G
Cuts at: 0 365 1647
Size: 365 1282
SrfI GCCC'GGGC
Cuts at: 0 27 1647
Size: 27 1620
StyI C'CwwG_G
Cuts at: 0 996 1394 1647
Size: 996 398 253
Fragments arranged by size:
996 398 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 966 1489 1647
Size: 966 523 158
Fragments arranged by size:
966 523 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 1647
Size: 60 32 1555
Fragments arranged by size:
1555 60 32
XbaI T'CTAG_A
Cuts at: 0 1536 1647
Size: 1536 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1098 1647
Size: 1098 549
XhoI C'TCGA_G
Cuts at: 0 365 1647
Size: 365 1282
XmaI C'CCGG_G
Cuts at: 0 25 1647
Size: 25 1622
Enzymes that do cut:
AarI AccI AcuI AlwNI AseI AvaI BanI BanII
BbvCI BclI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsmI Bsp1286I
BspMI BsrBI BsrDI BstAPI BstXI Bsu36I BtsI CspCI
DraIII EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
MfeI MmeI MspA1I PflMI PfoI PpiI PpuMI PspXI
PsrI PvuII SanDI SexAI SfcI SmaI SmlI SrfI
StyI TaqII TstI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BfrBI
BglII BmgBI BmtI BpuEI BsaAI BsaBI BsaHI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I BtgI BtgZI
ClaI DraI DrdI EaeI EagI EciI EcoICRI EcoRI
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PshAI PsiI PspOMI PstI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
SwaI TatI Tth111I XmnI ZraI