Restriction map for
pF1KA1434
(Linear) (Six-base) MAPSORT of: KIAA1434.seq Check: 6507 from: 1 to: 2016
KIAA1434.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 1909 2016
Size: 1706 203 107
Fragments arranged by size:
1706 203 107
AflII C'TTAA_G
Cuts at: 0 1321 1909 2016
Size: 1321 588 107
Fragments arranged by size:
1321 588 107
AflIII A'CryG_T
Cuts at: 0 382 750 2016
Size: 382 368 1266
Fragments arranged by size:
1266 382 368
AleI CACnn'nnGTG
Cuts at: 0 350 1234 2016
Size: 350 884 782
Fragments arranged by size:
884 782 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1539 2016
Size: 1539 477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 2016
Size: 173 32 1811
Fragments arranged by size:
1811 173 32
ApoI r'AATT_y
Cuts at: 0 809 837 1072 1150 1181 1287 1476 2016
Size: 809 28 235 78 31 106 189 540
Fragments arranged by size:
809 540 235 189 106 78 31 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 891 924 2016
Size: 891 33 1092
Fragments arranged by size:
1092 891 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1694 2016
Size: 1694 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 616 650 2016
Size: 616 34 1366
Fragments arranged by size:
1366 616 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 892 2016
Size: 892 1124
BglI GCCn_nnn'nGGC
Cuts at: 0 1003 2016
Size: 1003 1013
BglII A'GATC_T
Cuts at: 0 708 1615 2016
Size: 708 907 401
Fragments arranged by size:
907 708 401
Bme1580I G_kGCm'C
Cuts at: 0 1106 2016
Size: 1106 910
BmrI ACTGGGnnnn_n'
Cuts at: 0 1675 2016
Size: 1675 341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1198 2016
Size: 517 681 818
Fragments arranged by size:
818 681 517
Bpu10I CC'TnA_GC
Cuts at: 0 1730 2016
Size: 1730 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1559 2016
Size: 1559 457
BsaAI yAC'GTr
Cuts at: 0 723 2016
Size: 723 1293
BsaBI GATnn'nnATC
Cuts at: 0 1160 2016
Size: 1160 856
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 173 203 2016
Size: 173 30 1813
Fragments arranged by size:
1813 173 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 1503 2016
Size: 194 1309 513
Fragments arranged by size:
1309 513 194
BseYI C'CCAG_C
Cuts at: 0 998 1751 2016
Size: 998 753 265
Fragments arranged by size:
998 753 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 995 1079 1226 2016
Size: 612 383 84 147 790
Fragments arranged by size:
790 612 383 147 84
BsiHKAI G_wGCw'C
Cuts at: 0 722 2016
Size: 722 1294
BsmI GAATG_Cn'
Cuts at: 0 597 2016
Size: 597 1419
Bsp1286I G_dGCh'C
Cuts at: 0 722 1106 2016
Size: 722 384 910
Fragments arranged by size:
910 722 384
BspHI T'CATG_A
Cuts at: 0 827 1116 2016
Size: 827 289 900
Fragments arranged by size:
900 827 289
BsrDI GCAATG_nn'
Cuts at: 0 782 1631 1643 2016
Size: 782 849 12 373
Fragments arranged by size:
849 782 373 12
BsrGI T'GTAC_A
Cuts at: 0 1677 2016
Size: 1677 339
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1899 2016
Size: 1899 117
BstBI TT'CG_AA
Cuts at: 0 701 2016
Size: 701 1315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 2016
Size: 555 1461
BstYI r'GATC_y
Cuts at: 0 708 1379 1615 2016
Size: 708 671 236 401
Fragments arranged by size:
708 671 401 236
BtgI C'CryG_G
Cuts at: 0 1106 2016
Size: 1106 910
DraI TTT'AAA
Cuts at: 0 1460 2016
Size: 1460 556
EaeI y'GGCC_r
Cuts at: 0 963 2016
Size: 963 1053
EarI CTCTTCn'nnn_
Cuts at: 0 122 824 1480 2016
Size: 122 702 656 536
Fragments arranged by size:
702 656 536 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1198 1706 1909 2016
Size: 517 681 508 203 107
Fragments arranged by size:
681 517 508 203 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1090 1891 2016
Size: 1090 801 125
Fragments arranged by size:
1090 801 125
EcoRI G'AATT_C
Cuts at: 0 809 2016
Size: 809 1207
EcoRV GAT'ATC
Cuts at: 0 563 1974 2016
Size: 563 1411 42
Fragments arranged by size:
1411 563 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 505 537 901 933 1600 1632 2016
Size: 26 479 32 364 32 667 32 384
Fragments arranged by size:
667 479 384 364 32 32 32 26
HincII GTy'rAC
Cuts at: 0 870 2016
Size: 870 1146
MfeI C'AATT_G
Cuts at: 0 1876 2016
Size: 1876 140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 2016
Size: 619 1397
MscI TGG'CCA
Cuts at: 0 965 2016
Size: 965 1051
MslI CAynn'nnrTG
Cuts at: 0 350 971 1234 1636 1956 1982 2016
Size: 350 621 263 402 320 26 34
Fragments arranged by size:
621 402 350 320 263 34 26
MspA1I CmG'CkG
Cuts at: 0 1002 2016
Size: 1002 1014
NspI r_CATG'y
Cuts at: 0 150 386 754 2016
Size: 150 236 368 1262
Fragments arranged by size:
1262 368 236 150
PciI A'CATG_T
Cuts at: 0 382 750 2016
Size: 382 368 1266
Fragments arranged by size:
1266 382 368
PflMI CCAn_nnn'nTGG
Cuts at: 0 935 2016
Size: 935 1081
PfoI T'CCnGG_A
Cuts at: 0 744 841 2016
Size: 744 97 1175
Fragments arranged by size:
1175 744 97
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 2016
Size: 173 32 1811
Fragments arranged by size:
1811 173 32
PvuII CAG'CTG
Cuts at: 0 1002 2016
Size: 1002 1014
SapI GCTCTTCn'nnn_
Cuts at: 0 122 2016
Size: 122 1894
SmlI C'TyrA_G
Cuts at: 0 1321 1574 1909 2016
Size: 1321 253 335 107
Fragments arranged by size:
1321 335 253 107
SpeI A'CTAG_T
Cuts at: 0 1737 2016
Size: 1737 279
SphI G_CATG'C
Cuts at: 0 150 2016
Size: 150 1866
StuI AGG'CCT
Cuts at: 0 494 2016
Size: 494 1522
TatI w'GTAC_w
Cuts at: 0 168 531 647 1677 2016
Size: 168 363 116 1030 339
Fragments arranged by size:
1030 363 339 168 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 1387 1419 2016
Size: 529 32 826 32 597
Fragments arranged by size:
826 597 529 32 32
XbaI T'CTAG_A
Cuts at: 0 463 2016
Size: 463 1553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1393 2016
Size: 1393 623
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI AloI ApoI BaeI
BbsI BcgI BciVI BglI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI BstBI
BstXI BstYI BtgI DraI EaeI EarI Eco57MI EcoNI
EcoRI EcoRV Hin4I HincII MfeI MmeI MscI MslI
MspA1I NspI PciI PflMI PfoI PpiI PvuII SapI
SmlI SpeI SphI StuI TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BclI BfrBI BlpI
BmgBI BmtI BplI BsaI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoO109I FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI SspI StyI SwaI TaqII Tth111I
XhoI XmaI XmnI ZraI