Restriction map for
pF1KSDA1431
(Linear) (Six-base) MAPSORT of: KIAA1431.seq Check: 7878 from: 1 to: 2604
KIAA1431.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1524 1608 1692 1860 2028 2448 2604
Size: 974 550 84 84 168 168 420 156
Fragments arranged by size:
974 550 420 168 168 156 84 84
AleI CACnn'nnGTG
Cuts at: 0 2087 2604
Size: 2087 517
ApoI r'AATT_y
Cuts at: 0 750 1197 1319 1574 2498 2569 2604
Size: 750 447 122 255 924 71 35
Fragments arranged by size:
924 750 447 255 122 71 35
AvaI C'yCGr_G
Cuts at: 0 45 74 128 2604
Size: 45 29 54 2476
Fragments arranged by size:
2476 54 45 29
BanI G'GyrC_C
Cuts at: 0 55 515 2604
Size: 55 460 2089
Fragments arranged by size:
2089 460 55
BanII G_rGCy'C
Cuts at: 0 210 857 1297 2604
Size: 210 647 440 1307
Fragments arranged by size:
1307 647 440 210
BbeI G_GCGC'C
Cuts at: 0 59 2604
Size: 59 2545
BbsI GAAGACnn'nnnn_
Cuts at: 0 2454 2604
Size: 2454 150
BfrBI ATG'CAT
Cuts at: 0 1720 2604
Size: 1720 884
BglI GCCn_nnn'nGGC
Cuts at: 0 115 2604
Size: 115 2489
BglII A'GATC_T
Cuts at: 0 2431 2604
Size: 2431 173
BlpI GC'TnA_GC
Cuts at: 0 815 2604
Size: 815 1789
Bme1580I G_kGCm'C
Cuts at: 0 203 520 2604
Size: 203 317 2084
Fragments arranged by size:
2084 317 203
BmgBI CAC'GTC
Cuts at: 0 2543 2604
Size: 2543 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1508 1844 2604
Size: 663 845 336 760
Fragments arranged by size:
845 760 663 336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1316 2604
Size: 1316 1288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 277 649 1349 1503 1604 1772 1940
Size: 89 188 372 700 154 101 168 168
Cuts at: 1940 2276 2444 2528 2604
Size: 336 168 84 76
Fragments arranged by size:
700 372 336 188 168 168 168 154
101 89 84 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 544 2604
Size: 129 415 2060
Fragments arranged by size:
2060 415 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 910 2604
Size: 910 1694
BsaHI Gr'CG_yC
Cuts at: 0 37 56 2604
Size: 37 19 2548
Fragments arranged by size:
2548 37 19
BsaWI w'CCGG_w
Cuts at: 0 1666 2604
Size: 1666 938
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 2604
Size: 337 30 2237
Fragments arranged by size:
2237 337 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 358 447 2604
Size: 191 167 89 2157
Fragments arranged by size:
2157 191 167 89
BseYI C'CCAG_C
Cuts at: 0 423 738 2604
Size: 423 315 1866
Fragments arranged by size:
1866 423 315
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2149 2604
Size: 2149 455
BsiHKAI G_wGCw'C
Cuts at: 0 210 1297 2604
Size: 210 1087 1307
Fragments arranged by size:
1307 1087 210
BsmI GAATG_Cn'
Cuts at: 0 2035 2209 2278 2604
Size: 2035 174 69 326
Fragments arranged by size:
2035 326 174 69
Bsp1286I G_dGCh'C
Cuts at: 0 203 210 520 857 1297 2604
Size: 203 7 310 337 440 1307
Fragments arranged by size:
1307 440 337 310 203 7
BsrBI CCG'CTC
Cuts at: 0 42 181 2604
Size: 42 139 2423
Fragments arranged by size:
2423 139 42
BsrGI T'GTAC_A
Cuts at: 0 380 909 1647 2319 2604
Size: 380 529 738 672 285
Fragments arranged by size:
738 672 529 380 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2030 2604
Size: 2030 574
BstYI r'GATC_y
Cuts at: 0 2431 2556 2604
Size: 2431 125 48
Fragments arranged by size:
2431 125 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 404 2224 2604
Size: 404 1820 380
Fragments arranged by size:
1820 404 380
BtsI GCAGTG_nn'
Cuts at: 0 224 602 2283 2604
Size: 224 378 1681 321
Fragments arranged by size:
1681 378 321 224
ClaI AT'CG_AT
Cuts at: 0 1523 2604
Size: 1523 1081
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 328 363 2534 2569 2604
Size: 328 35 2171 35 35
Fragments arranged by size:
2171 328 35 35 35
EaeI y'GGCC_r
Cuts at: 0 2172 2604
Size: 2172 432
EarI CTCTTCn'nnn_
Cuts at: 0 236 1160 2604
Size: 236 924 1444
Fragments arranged by size:
1444 924 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 712 2604
Size: 712 1892
EcoICRI GAG'CTC
Cuts at: 0 208 1295 2604
Size: 208 1087 1309
Fragments arranged by size:
1309 1087 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 277 649 974 1349 1503 1524 1604
Size: 89 188 372 325 375 154 21 80
Cuts at: 1604 1608 1692 1772 1860 1940 2028 2276 2444
Size: 4 84 80 88 80 88 248 168
Cuts at: 2444 2448 2528 2604
Size: 4 80 76
Fragments arranged by size:
375 372 325 248 188 168 154 89
88 88 84 80 80 80 80 76
21 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 2604
Size: 1105 1499
EcoO109I rG'GnC_Cy
Cuts at: 0 193 2604
Size: 193 2411
EcoRI G'AATT_C
Cuts at: 0 1319 1574 2498 2604
Size: 1319 255 924 106
Fragments arranged by size:
1319 924 255 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 585 2604
Size: 585 2019
HaeII r_GCGC'y
Cuts at: 0 59 2604
Size: 59 2545
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 778 810 814 846 958 990
Size: 439 32 307 32 4 32 112 32
Cuts at: 990 1366 1398 1476 1508 2125 2157 2604
Size: 376 32 78 32 617 32 447
Fragments arranged by size:
617 447 439 376 307 112 78 32
32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 717 2519 2604
Size: 717 1802 85
Fragments arranged by size:
1802 717 85
HindIII A'AGCT_T
Cuts at: 0 1042 1252 1448 1787 2604
Size: 1042 210 196 339 817
Fragments arranged by size:
1042 817 339 210 196
KasI G'GCGC_C
Cuts at: 0 55 2604
Size: 55 2549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 443 2604
Size: 377 66 2161
Fragments arranged by size:
2161 377 66
MscI TGG'CCA
Cuts at: 0 2174 2604
Size: 2174 430
MslI CAynn'nnrTG
Cuts at: 0 1181 1683 2019 2087 2355 2604
Size: 1181 502 336 68 268 249
Fragments arranged by size:
1181 502 336 268 249 68
NarI GG'CG_CC
Cuts at: 0 56 2604
Size: 56 2548
NdeI CA'TA_TG
Cuts at: 0 948 1680 2016 2352 2604
Size: 948 732 336 336 252
Fragments arranged by size:
948 732 336 336 252
NsiI A_TGCA'T
Cuts at: 0 1722 2604
Size: 1722 882
NspI r_CATG'y
Cuts at: 0 2525 2604
Size: 2525 79
PasI CC'CwG_GG
Cuts at: 0 297 2604
Size: 297 2307
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 2604
Size: 303 2301
PsiI TTA'TAA
Cuts at: 0 1343 2438 2604
Size: 1343 1095 166
Fragments arranged by size:
1343 1095 166
SacI G_AGCT'C
Cuts at: 0 210 1297 2604
Size: 210 1087 1307
Fragments arranged by size:
1307 1087 210
SapI GCTCTTCn'nnn_
Cuts at: 0 236 2604
Size: 236 2368
ScaI AGT'ACT
Cuts at: 0 634 2604
Size: 634 1970
SfcI C'TryA_G
Cuts at: 0 95 326 405 2604
Size: 95 231 79 2199
Fragments arranged by size:
2199 231 95 79
SfoI GGC'GCC
Cuts at: 0 57 2604
Size: 57 2547
SmaI CCC'GGG
Cuts at: 0 47 76 130 2604
Size: 47 29 54 2474
Fragments arranged by size:
2474 54 47 29
SmlI C'TyrA_G
Cuts at: 0 144 559 2604
Size: 144 415 2045
Fragments arranged by size:
2045 415 144
StuI AGG'CCT
Cuts at: 0 1366 2125 2604
Size: 1366 759 479
Fragments arranged by size:
1366 759 479
StyI C'CwwG_G
Cuts at: 0 113 481 2604
Size: 113 368 2123
Fragments arranged by size:
2123 368 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 752 1978 2604
Size: 752 1226 626
Fragments arranged by size:
1226 752 626
TatI w'GTAC_w
Cuts at: 0 380 632 909 1647 1743 2146 2319 2604
Size: 380 252 277 738 96 403 173 285
Fragments arranged by size:
738 403 380 285 277 252 173 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 337 369 794 826 2532 2564
Size: 216 32 89 32 425 32 1706 32
Cuts at: 2564 2604
Size: 40
Fragments arranged by size:
1706 425 216 89 40 32 32 32
32
XmaI C'CCGG_G
Cuts at: 0 45 74 128 2604
Size: 45 29 54 2476
Fragments arranged by size:
2476 54 45 29
XmnI GAAnn'nnTTC
Cuts at: 0 568 751 929 1450 1537 2334 2377 2604
Size: 568 183 178 521 87 797 43 227
Fragments arranged by size:
797 568 521 227 183 178 87 43
Enzymes that do cut:
AcuI AleI ApoI AvaI BanI BanII BbeI BbsI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrGI BstAPI BstYI
BtgZI BtsI ClaI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI NarI NdeI NsiI
NspI PasI PflMI PsiI SacI SapI ScaI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BmtI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI DraI DraIII DrdI
EagI EcoRV FseI FspI FspAI HpaI KpnI MfeI
MluI MspA1I NaeI NcoI NgoMIV NheI NotI NruI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI ZraI