Restriction map for
pF1KA1428
(Linear) (Six-base) MAPSORT of: KIAA1428.seq Check: 986 from: 1 to: 2127
KIAA1428.orf
With 182 enzymes: SgfI *
October 16, 2009 16:19 ..
AccI GT'mk_AC
Cuts at: 0 1303 2022 2127
Size: 1303 719 105
Fragments arranged by size:
1303 719 105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1499 2127
Size: 547 952 628
Fragments arranged by size:
952 628 547
AflII C'TTAA_G
Cuts at: 0 1134 2127
Size: 1134 993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 680 712 2127
Size: 680 32 1415
Fragments arranged by size:
1415 680 32
ApoI r'AATT_y
Cuts at: 0 670 808 825 921 1308 2127
Size: 670 138 17 96 387 819
Fragments arranged by size:
819 670 387 138 96 17
AseI AT'TA_AT
Cuts at: 0 431 2127
Size: 431 1696
AvaI C'yCGr_G
Cuts at: 0 1592 2127
Size: 1592 535
BamHI G'GATC_C
Cuts at: 0 1285 2127
Size: 1285 842
BanII G_rGCy'C
Cuts at: 0 250 2127
Size: 250 1877
BbsI GAAGACnn'nnnn_
Cuts at: 0 1013 2127
Size: 1013 1114
BciVI GTATCCnnnnn_n'
Cuts at: 0 838 2127
Size: 838 1289
BclI T'GATC_A
Cuts at: 0 414 2127
Size: 414 1713
BfrBI ATG'CAT
Cuts at: 0 132 537 2127
Size: 132 405 1590
Fragments arranged by size:
1590 405 132
BglI GCCn_nnn'nGGC
Cuts at: 0 1424 2127
Size: 1424 703
BglII A'GATC_T
Cuts at: 0 363 2127
Size: 363 1764
BlpI GC'TnA_GC
Cuts at: 0 286 2127
Size: 286 1841
BmrI ACTGGGnnnn_n'
Cuts at: 0 342 2127
Size: 342 1785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 306 2127
Size: 306 1821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1475 2127
Size: 58 1417 652
Fragments arranged by size:
1417 652 58
Bpu10I CC'TnA_GC
Cuts at: 0 2062 2127
Size: 2062 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 727 2127
Size: 727 1400
BsaBI GATnn'nnATC
Cuts at: 0 1547 2127
Size: 1547 580
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 2127
Size: 43 2084
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1145 2127
Size: 185 960 982
Fragments arranged by size:
982 960 185
BsiHKAI G_wGCw'C
Cuts at: 0 250 2127
Size: 250 1877
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 2127
Size: 25 2102
Bsp1286I G_dGCh'C
Cuts at: 0 250 2127
Size: 250 1877
BspHI T'CATG_A
Cuts at: 0 417 1086 2127
Size: 417 669 1041
Fragments arranged by size:
1041 669 417
BsrDI GCAATG_nn'
Cuts at: 0 253 2127
Size: 253 1874
BsrGI T'GTAC_A
Cuts at: 0 500 1102 2127
Size: 500 602 1025
Fragments arranged by size:
1025 602 500
BssSI C'ACGA_G
Cuts at: 0 1163 1222 2127
Size: 1163 59 905
Fragments arranged by size:
1163 905 59
BstYI r'GATC_y
Cuts at: 0 363 1097 1285 1294 2127
Size: 363 734 188 9 833
Fragments arranged by size:
833 734 363 188 9
Bsu36I CC'TnA_GG
Cuts at: 0 1290 2127
Size: 1290 837
BtgI C'CryG_G
Cuts at: 0 939 965 2127
Size: 939 26 1162
Fragments arranged by size:
1162 939 26
BtsI GCAGTG_nn'
Cuts at: 0 308 2127
Size: 308 1819
DraI TTT'AAA
Cuts at: 0 194 354 1655 2127
Size: 194 160 1301 472
Fragments arranged by size:
1301 472 194 160
EaeI y'GGCC_r
Cuts at: 0 962 1234 1255 1398 1416 2127
Size: 962 272 21 143 18 711
Fragments arranged by size:
962 711 272 143 21 18
EarI CTCTTCn'nnn_
Cuts at: 0 566 1085 2075 2104 2127
Size: 566 519 990 29 23
Fragments arranged by size:
990 566 519 29 23
EcoICRI GAG'CTC
Cuts at: 0 248 2127
Size: 248 1879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 547 1475 1499 2127
Size: 58 489 928 24 628
Fragments arranged by size:
928 628 489 58 24
HaeII r_GCGC'y
Cuts at: 0 1746 2127
Size: 1746 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 2127
Size: 1522 32 573
Fragments arranged by size:
1522 573 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1836 2127
Size: 125 1711 291
Fragments arranged by size:
1711 291 125
MscI TGG'CCA
Cuts at: 0 964 1400 1418 2127
Size: 964 436 18 709
Fragments arranged by size:
964 709 436 18
MslI CAynn'nnrTG
Cuts at: 0 1553 2127
Size: 1553 574
NcoI C'CATG_G
Cuts at: 0 965 2127
Size: 965 1162
NsiI A_TGCA'T
Cuts at: 0 134 539 2127
Size: 134 405 1588
Fragments arranged by size:
1588 405 134
NspI r_CATG'y
Cuts at: 0 612 1885 2127
Size: 612 1273 242
Fragments arranged by size:
1273 612 242
SacI G_AGCT'C
Cuts at: 0 250 2127
Size: 250 1877
SmaI CCC'GGG
Cuts at: 0 1594 2127
Size: 1594 533
SmlI C'TyrA_G
Cuts at: 0 1134 2127
Size: 1134 993
SphI G_CATG'C
Cuts at: 0 1885 2127
Size: 1885 242
SrfI GCCC'GGGC
Cuts at: 0 1594 2127
Size: 1594 533
SspI AAT'ATT
Cuts at: 0 684 2127
Size: 684 1443
StuI AGG'CCT
Cuts at: 0 959 2127
Size: 959 1168
StyI C'CwwG_G
Cuts at: 0 965 1518 2127
Size: 965 553 609
Fragments arranged by size:
965 609 553
TatI w'GTAC_w
Cuts at: 0 500 566 1102 1462 2127
Size: 500 66 536 360 665
Fragments arranged by size:
665 536 500 360 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 795 827 2127
Size: 795 32 1300
Fragments arranged by size:
1300 795 32
Tth111I GACn'n_nGTC
Cuts at: 0 57 2127
Size: 57 2070
XbaI T'CTAG_A
Cuts at: 0 1326 2127
Size: 1326 801
XmaI C'CCGG_G
Cuts at: 0 1592 2127
Size: 1592 535
XmnI GAAnn'nnTTC
Cuts at: 0 395 2127
Size: 395 1732
Enzymes that do cut:
AccI AcuI AflII AloI ApoI AseI AvaI BamHI
BanII BbsI BciVI BclI BfrBI BglI BglII BlpI
BmrI BplI BpmI Bpu10I BsaI BsaBI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrGI BssSI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
HaeII Hin4I MmeI MscI MslI NcoI NsiI NspI
SacI SmaI SmlI SphI SrfI SspI StuI StyI
TatI TstI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI Bme1580I BmgBI BmtI
BpuEI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmI BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SwaI TaqII XcmI XhoI ZraI