Restriction map for
pF1KA1405
(Linear) (Six-base) MAPSORT of: KIAA1405.seq Check: 8820 from: 1 to: 3087
KIAA1405.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1128 1149 1383 1772 2208 2577 3087
Size: 1128 21 234 389 436 369 510
Fragments arranged by size:
1128 510 436 389 369 234 21
AatII G_ACGT'C
Cuts at: 0 1340 1919 3087
Size: 1340 579 1168
Fragments arranged by size:
1340 1168 579
AccI GT'mk_AC
Cuts at: 0 2399 3087
Size: 2399 688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 2308 3087
Size: 502 1806 779
Fragments arranged by size:
1806 779 502
AfeI AGC'GCT
Cuts at: 0 1449 2314 3087
Size: 1449 865 773
Fragments arranged by size:
1449 865 773
AflIII A'CryG_T
Cuts at: 0 359 3087
Size: 359 2728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1958 3087
Size: 1958 1129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 570 601 602 633 3087
Size: 570 31 1 31 2454
Fragments arranged by size:
2454 570 31 31 1
AlwNI CAG_nnn'CTG
Cuts at: 0 309 474 1153 1384 2071 2223 2580 2598
Size: 309 165 679 231 687 152 357 18
Cuts at: 2598 2612 3087
Size: 14 475
Fragments arranged by size:
687 679 475 357 309 231 165 152
18 14
ApaLI G'TGCA_C
Cuts at: 0 535 3087
Size: 535 2552
AvaI C'yCGr_G
Cuts at: 0 123 1289 1741 3013 3087
Size: 123 1166 452 1272 74
Fragments arranged by size:
1272 1166 452 123 74
BamHI G'GATC_C
Cuts at: 0 315 3087
Size: 315 2772
BanI G'GyrC_C
Cuts at: 0 127 166 253 1140 1760 1809 3087
Size: 127 39 87 887 620 49 1278
Fragments arranged by size:
1278 887 620 127 87 49 39
BanII G_rGCy'C
Cuts at: 0 1108 1612 1676 1967 2126 3087
Size: 1108 504 64 291 159 961
Fragments arranged by size:
1108 961 504 291 159 64
BbeI G_GCGC'C
Cuts at: 0 131 170 3087
Size: 131 39 2917
Fragments arranged by size:
2917 131 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 1313 1491 3087
Size: 1313 178 1596
Fragments arranged by size:
1596 1313 178
BbvCI CC'TCA_GC
Cuts at: 0 1415 2884 2935 3087
Size: 1415 1469 51 152
Fragments arranged by size:
1469 1415 152 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 643 677 1270 1304 1938 1972 3087
Size: 643 34 593 34 634 34 1115
Fragments arranged by size:
1115 643 634 593 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 10 278 991 1867 1993 2101 2110 3087
Size: 10 268 713 876 126 108 9 977
Fragments arranged by size:
977 876 713 268 126 108 10 9
BglII A'GATC_T
Cuts at: 0 197 422 2025 2490 3087
Size: 197 225 1603 465 597
Fragments arranged by size:
1603 597 465 225 197
BlpI GC'TnA_GC
Cuts at: 0 2114 3087
Size: 2114 973
Bme1580I G_kGCm'C
Cuts at: 0 539 1145 1763 1814 3087
Size: 539 606 618 51 1273
Fragments arranged by size:
1273 618 606 539 51
BmgBI CAC'GTC
Cuts at: 0 186 852 3087
Size: 186 666 2235
Fragments arranged by size:
2235 666 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 543 817 907 1517 1961 2391 2710
Size: 99 444 274 90 610 444 430 319
Cuts at: 2710 2741 3087
Size: 31 346
Fragments arranged by size:
610 444 444 430 346 319 274 99
90 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1641 1692 2574 3087
Size: 1641 51 882 513
Fragments arranged by size:
1641 882 513 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 496 1321 1375 1539 1596 1981 2464
Size: 439 57 825 54 164 57 385 483
Cuts at: 2464 2533 2602 2650 3087
Size: 69 69 48 437
Fragments arranged by size:
825 483 439 437 385 164 69 69
57 57 54 48
Bpu10I CC'TnA_GC
Cuts at: 0 1415 2090 2120 2884 2935 3087
Size: 1415 675 30 764 51 152
Fragments arranged by size:
1415 764 675 152 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1063 3087
Size: 754 309 2024
Fragments arranged by size:
2024 754 309
BsaI GGTCTCn'nnnn_
Cuts at: 0 1695 3087
Size: 1695 1392
BsaAI yAC'GTr
Cuts at: 0 177 360 510 3087
Size: 177 183 150 2577
Fragments arranged by size:
2577 183 177 150
BsaHI Gr'CG_yC
Cuts at: 0 128 167 233 1337 1916 2894 3087
Size: 128 39 66 1104 579 978 193
Fragments arranged by size:
1104 978 579 193 128 66 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1159 1189 1519 1549 2817 2847 3087
Size: 1159 30 330 30 1268 30 240
Fragments arranged by size:
1268 1159 330 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1078 1180 1246 1372 1684 1720 1810 1869
Size: 1078 102 66 126 312 36 90 59
Cuts at: 1869 2809 3087
Size: 940 278
Fragments arranged by size:
1078 940 312 278 126 102 90 66
59 36
BseYI C'CCAG_C
Cuts at: 0 1108 1756 1767 2654 2993 3087
Size: 1108 648 11 887 339 94
Fragments arranged by size:
1108 887 648 339 94 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1177 1954 2611 3087
Size: 401 776 777 657 476
Fragments arranged by size:
777 776 657 476 401
BsiEI CG_ry'CG
Cuts at: 0 593 3087
Size: 593 2494
BsiHKAI G_wGCw'C
Cuts at: 0 359 491 539 560 3087
Size: 359 132 48 21 2527
Fragments arranged by size:
2527 359 132 48 21
Bsp1286I G_dGCh'C
Cuts at: 0 359 491 539 560 1108 1145 1612 1676
Size: 359 132 48 21 548 37 467 64
Cuts at: 1676 1763 1814 1967 2126 3087
Size: 87 51 153 159 961
Fragments arranged by size:
961 548 467 359 159 153 132 87
64 51 48 37 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1128 1149 1373 1383 1772 1926 1964 2208
Size: 1128 21 224 10 389 154 38 244
Cuts at: 2208 2241 2577 3087
Size: 33 336 510
Fragments arranged by size:
1128 510 389 336 244 224 154 38
33 21 10
BsrBI CCG'CTC
Cuts at: 0 675 735 765 3087
Size: 675 60 30 2322
Fragments arranged by size:
2322 675 60 30
BsrDI GCAATG_nn'
Cuts at: 0 819 2840 3087
Size: 819 2021 247
Fragments arranged by size:
2021 819 247
BsrFI r'CCGG_y
Cuts at: 0 2130 2543 3087
Size: 2130 413 544
Fragments arranged by size:
2130 544 413
BssHII G'CGCG_C
Cuts at: 0 95 97 99 3087
Size: 95 2 2 2988
Fragments arranged by size:
2988 95 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 534 1809 2220 3087
Size: 309 225 1275 411 867
Fragments arranged by size:
1275 867 411 309 225
BstYI r'GATC_y
Cuts at: 0 197 315 422 2025 2490 2681 2879 3087
Size: 197 118 107 1603 465 191 198 208
Fragments arranged by size:
1603 465 208 198 197 191 118 107
Bsu36I CC'TnA_GG
Cuts at: 0 1667 2018 3087
Size: 1667 351 1069
Fragments arranged by size:
1667 1069 351
BtgI C'CryG_G
Cuts at: 0 77 665 755 1886 3087
Size: 77 588 90 1131 1201
Fragments arranged by size:
1201 1131 588 90 77
BtsI GCAGTG_nn'
Cuts at: 0 1313 2183 3081 3087
Size: 1313 870 898 6
Fragments arranged by size:
1313 898 870 6
ClaI AT'CG_AT
Cuts at: 0 2522 3087
Size: 2522 565
DraIII CAC_nnn'GTG
Cuts at: 0 534 543 3087
Size: 534 9 2544
Fragments arranged by size:
2544 534 9
EaeI y'GGCC_r
Cuts at: 0 270 1868 1889 2132 2531 3087
Size: 270 1598 21 243 399 556
Fragments arranged by size:
1598 556 399 270 243 21
EarI CTCTTCn'nnn_
Cuts at: 0 1235 2143 2896 3087
Size: 1235 908 753 191
Fragments arranged by size:
1235 908 753 191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 308 2530 2998 3087
Size: 308 2222 468 89
Fragments arranged by size:
2222 468 308 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 496 502 1321 1375 1539 1596 1981
Size: 439 57 6 819 54 164 57 385
Cuts at: 1981 2308 2464 2533 2602 2650 3087
Size: 327 156 69 69 48 437
Fragments arranged by size:
819 439 437 385 327 164 156 69
69 57 57 54 48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1369 1408 1744 2584 3087
Size: 1369 39 336 840 503
Fragments arranged by size:
1369 840 503 336 39
EcoO109I rG'GnC_Cy
Cuts at: 0 306 347 444 1029 1839 1926 1938 2094
Size: 306 41 97 585 810 87 12 156
Cuts at: 2094 2283 2979 3087
Size: 189 696 108
Fragments arranged by size:
810 696 585 306 189 156 108 97
87 41 12
FspI TGC'GCA
Cuts at: 0 1324 3087
Size: 1324 1763
HaeII r_GCGC'y
Cuts at: 0 131 170 829 843 1248 1451 2316 3087
Size: 131 39 659 14 405 203 865 771
Fragments arranged by size:
865 771 659 405 203 131 39 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 307 323 339 355 724 756
Size: 201 32 74 16 16 16 369 32
Cuts at: 756 1270 1302 1408 1440 1500 1519 1532 1551
Size: 514 32 106 32 60 19 13 19
Cuts at: 1551 2017 2049 2475 2482 2507 2514 2673 2705
Size: 466 32 426 7 25 7 159 32
Cuts at: 2705 3087
Size: 382
Fragments arranged by size:
514 466 426 382 369 201 159 106
74 60 32 32 32 32 32 32
25 19 19 16 16 16 13 7
7
HindIII A'AGCT_T
Cuts at: 0 2461 3087
Size: 2461 626
KasI G'GCGC_C
Cuts at: 0 127 166 3087
Size: 127 39 2921
Fragments arranged by size:
2921 127 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1703 2063 2222 2803 3087
Size: 1703 360 159 581 284
Fragments arranged by size:
1703 581 360 284 159
MscI TGG'CCA
Cuts at: 0 1870 1891 2533 3087
Size: 1870 21 642 554
Fragments arranged by size:
1870 642 554 21
MslI CAynn'nnrTG
Cuts at: 0 604 655 1318 2170 3087
Size: 604 51 663 852 917
Fragments arranged by size:
917 852 663 604 51
MspA1I CmG'CkG
Cuts at: 0 51 222 284 1221 1756 2140 2196 2238
Size: 51 171 62 937 535 384 56 42
Cuts at: 2238 2340 2375 2511 2939 3087
Size: 102 35 136 428 148
Fragments arranged by size:
937 535 428 384 171 148 136 102
62 56 51 42 35
NaeI GCC'GGC
Cuts at: 0 2132 2545 3087
Size: 2132 413 542
Fragments arranged by size:
2132 542 413
NarI GG'CG_CC
Cuts at: 0 128 167 3087
Size: 128 39 2920
Fragments arranged by size:
2920 128 39
NdeI CA'TA_TG
Cuts at: 0 1332 3087
Size: 1332 1755
NgoMIV G'CCGG_C
Cuts at: 0 2130 2543 3087
Size: 2130 413 544
Fragments arranged by size:
2130 544 413
NruI TCG'CGA
Cuts at: 0 1052 3087
Size: 1052 2035
PasI CC'CwG_GG
Cuts at: 0 344 1660 3087
Size: 344 1316 1427
Fragments arranged by size:
1427 1316 344
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 3087
Size: 78 3009
PfoI T'CCnGG_A
Cuts at: 0 440 1226 1711 1727 2411 2579 3087
Size: 440 786 485 16 684 168 508
Fragments arranged by size:
786 684 508 485 440 168 16
PmlI CAC'GTG
Cuts at: 0 177 360 3087
Size: 177 183 2727
Fragments arranged by size:
2727 183 177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 3087
Size: 346 32 2709
Fragments arranged by size:
2709 346 32
PpuMI rG'GwC_Cy
Cuts at: 0 444 1029 1839 1926 1938 2283 3087
Size: 444 585 810 87 12 345 804
Fragments arranged by size:
810 804 585 444 345 87 12
PspXI vC'TCGA_Gb
Cuts at: 0 3013 3087
Size: 3013 74
PstI C_TGCA'G
Cuts at: 0 890 1394 1820 1838 2237 2357 2498 2769
Size: 890 504 426 18 399 120 141 271
Cuts at: 2769 3087
Size: 318
Fragments arranged by size:
890 504 426 399 318 271 141 120
18
PvuII CAG'CTG
Cuts at: 0 1221 2140 2238 2340 2511 2939 3087
Size: 1221 919 98 102 171 428 148
Fragments arranged by size:
1221 919 428 171 148 102 98
SapI GCTCTTCn'nnn_
Cuts at: 0 1235 2896 3087
Size: 1235 1661 191
Fragments arranged by size:
1661 1235 191
SbfI CC_TGCA'GG
Cuts at: 0 1394 1820 1838 3087
Size: 1394 426 18 1249
Fragments arranged by size:
1394 1249 426 18
SexAI A'CCwGG_T
Cuts at: 0 710 3087
Size: 710 2377
SfcI C'TryA_G
Cuts at: 0 886 1390 1816 1834 2233 2353 2494 2619
Size: 886 504 426 18 399 120 141 125
Cuts at: 2619 2765 2805 3087
Size: 146 40 282
Fragments arranged by size:
886 504 426 399 282 146 141 125
120 40 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2101 3087
Size: 2101 986
SfoI GGC'GCC
Cuts at: 0 129 168 3087
Size: 129 39 2919
Fragments arranged by size:
2919 129 39
SmaI CCC'GGG
Cuts at: 0 125 3087
Size: 125 2962
SmlI C'TyrA_G
Cuts at: 0 769 1078 3013 3087
Size: 769 309 1935 74
Fragments arranged by size:
1935 769 309 74
StuI AGG'CCT
Cuts at: 0 1832 2016 2933 3087
Size: 1832 184 917 154
Fragments arranged by size:
1832 917 184 154
StyI C'CwwG_G
Cuts at: 0 450 1034 1493 1574 1922 3038 3087
Size: 450 584 459 81 348 1116 49
Fragments arranged by size:
1116 584 459 450 348 81 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 506 579 2248 2373 2985 2989 3087
Size: 506 73 1669 125 612 4 98
Fragments arranged by size:
1669 612 506 125 98 73 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2817 2849 3087
Size: 2817 32 238
Fragments arranged by size:
2817 238 32
Tth111I GACn'n_nGTC
Cuts at: 0 184 1531 3087
Size: 184 1347 1556
Fragments arranged by size:
1556 1347 184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 918 3087
Size: 471 447 2169
Fragments arranged by size:
2169 471 447
XhoI C'TCGA_G
Cuts at: 0 3013 3087
Size: 3013 74
XmaI C'CCGG_G
Cuts at: 0 123 3087
Size: 123 2964
XmnI GAAnn'nnTTC
Cuts at: 0 292 3087
Size: 292 2795
ZraI GAC'GTC
Cuts at: 0 1338 1917 3087
Size: 1338 579 1170
Fragments arranged by size:
1338 1170 579
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AlfI AloI
AlwNI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstYI Bsu36I BtgI BtsI
ClaI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NruI PasI PflMI
PfoI PmlI PpiI PpuMI PspXI PstI PvuII SapI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI
BmtI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgZI CspCI
DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HincII HpaI KpnI MfeI MluI NcoI NheI
NotI NsiI NspI PacI PciI PmeI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI