Restriction map for
pF1KA1390
(Linear) (Six-base) MAPSORT of: KIAA1390.seq Check: 4658 from: 1 to: 1407
KIAA1390.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 808 1407
Size: 808 599
AccI GT'mk_AC
Cuts at: 0 664 1166 1407
Size: 664 502 241
Fragments arranged by size:
664 502 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 278 427 541 1240 1407
Size: 233 45 149 114 699 167
Fragments arranged by size:
699 233 167 149 114 45
AleI CACnn'nnGTG
Cuts at: 0 672 812 1407
Size: 672 140 595
Fragments arranged by size:
672 595 140
AlwNI CAG_nnn'CTG
Cuts at: 0 31 858 913 1407
Size: 31 827 55 494
Fragments arranged by size:
827 494 55 31
AvaI C'yCGr_G
Cuts at: 0 670 1407
Size: 670 737
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 691 724 1407
Size: 691 33 683
Fragments arranged by size:
691 683 33
BamHI G'GATC_C
Cuts at: 0 21 353 1407
Size: 21 332 1054
Fragments arranged by size:
1054 332 21
BanII G_rGCy'C
Cuts at: 0 497 1407
Size: 497 910
BclI T'GATC_A
Cuts at: 0 479 1407
Size: 479 928
BlpI GC'TnA_GC
Cuts at: 0 222 1407
Size: 222 1185
BmtI G_CTAG'C
Cuts at: 0 221 1407
Size: 221 1186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 880 1142 1407
Size: 795 85 262 265
Fragments arranged by size:
795 265 262 85
Bpu10I CC'TnA_GC
Cuts at: 0 326 917 1407
Size: 326 591 490
Fragments arranged by size:
591 490 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1407
Size: 217 1190
BsaI GGTCTCn'nnnn_
Cuts at: 0 856 1407
Size: 856 551
BsaHI Gr'CG_yC
Cuts at: 0 647 1350 1407
Size: 647 703 57
Fragments arranged by size:
703 647 57
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1039 1279 1349 1407
Size: 1039 240 70 58
Fragments arranged by size:
1039 240 70 58
BseYI C'CCAG_C
Cuts at: 0 875 1221 1247 1353 1407
Size: 875 346 26 106 54
Fragments arranged by size:
875 346 106 54 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 773 1033 1407
Size: 696 77 260 374
Fragments arranged by size:
696 374 260 77
BsiHKAI G_wGCw'C
Cuts at: 0 101 397 497 1407
Size: 101 296 100 910
Fragments arranged by size:
910 296 101 100
BsmI GAATG_Cn'
Cuts at: 0 1397 1407
Size: 1397 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 654 1407
Size: 654 753
Bsp1286I G_dGCh'C
Cuts at: 0 101 397 497 1407
Size: 101 296 100 910
Fragments arranged by size:
910 296 101 100
BspHI T'CATG_A
Cuts at: 0 425 1407
Size: 425 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 698 808 1152 1407
Size: 698 110 344 255
Fragments arranged by size:
698 344 255 110
BsrDI GCAATG_nn'
Cuts at: 0 596 1407
Size: 596 811
BssSI C'ACGA_G
Cuts at: 0 826 1211 1384 1407
Size: 826 385 173 23
Fragments arranged by size:
826 385 173 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 124 1296 1407
Size: 124 1172 111
Fragments arranged by size:
1172 124 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 1407
Size: 621 786
BstYI r'GATC_y
Cuts at: 0 21 353 1407
Size: 21 332 1054
Fragments arranged by size:
1054 332 21
BstZ17I GTA'TAC
Cuts at: 0 665 1407
Size: 665 742
Bsu36I CC'TnA_GG
Cuts at: 0 17 104 740 1407
Size: 17 87 636 667
Fragments arranged by size:
667 636 87 17
BtgI C'CryG_G
Cuts at: 0 711 869 888 1407
Size: 711 158 19 519
Fragments arranged by size:
711 519 158 19
BtsI GCAGTG_nn'
Cuts at: 0 250 684 1321 1407
Size: 250 434 637 86
Fragments arranged by size:
637 434 250 86
DraIII CAC_nnn'GTG
Cuts at: 0 1056 1407
Size: 1056 351
EaeI y'GGCC_r
Cuts at: 0 611 1407
Size: 611 796
EcoICRI GAG'CTC
Cuts at: 0 495 1407
Size: 495 912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 278 427 541 795 880 1142 1240
Size: 233 45 149 114 254 85 262 98
Cuts at: 1240 1407
Size: 167
Fragments arranged by size:
262 254 233 167 149 114 98 85
45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 362 1111 1407
Size: 362 749 296
Fragments arranged by size:
749 362 296
EcoO109I rG'GnC_Cy
Cuts at: 0 314 1117 1314 1407
Size: 314 803 197 93
Fragments arranged by size:
803 314 197 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1002 1407
Size: 1002 405
HindIII A'AGCT_T
Cuts at: 0 164 1407
Size: 164 1243
MscI TGG'CCA
Cuts at: 0 613 1407
Size: 613 794
MslI CAynn'nnrTG
Cuts at: 0 672 812 1407
Size: 672 140 595
Fragments arranged by size:
672 595 140
MspA1I CmG'CkG
Cuts at: 0 780 871 879 913 1070 1158 1221 1407
Size: 780 91 8 34 157 88 63 186
Fragments arranged by size:
780 186 157 91 88 63 34 8
NcoI C'CATG_G
Cuts at: 0 711 1407
Size: 711 696
NheI G'CTAG_C
Cuts at: 0 217 1407
Size: 217 1190
NspI r_CATG'y
Cuts at: 0 281 1407
Size: 281 1126
PasI CC'CwG_GG
Cuts at: 0 1107 1407
Size: 1107 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 31 244 783 1407
Size: 31 213 539 624
Fragments arranged by size:
624 539 213 31
PfoI T'CCnGG_A
Cuts at: 0 857 1407
Size: 857 550
PpuMI rG'GwC_Cy
Cuts at: 0 314 1314 1407
Size: 314 1000 93
Fragments arranged by size:
1000 314 93
PshAI GACnn'nnGTC
Cuts at: 0 1312 1407
Size: 1312 95
PstI C_TGCA'G
Cuts at: 0 51 1163 1199 1328 1407
Size: 51 1112 36 129 79
Fragments arranged by size:
1112 129 79 51 36
PvuII CAG'CTG
Cuts at: 0 780 879 913 1070 1158 1407
Size: 780 99 34 157 88 249
Fragments arranged by size:
780 249 157 99 88 34
SacI G_AGCT'C
Cuts at: 0 497 1407
Size: 497 910
SacII CC_GC'GG
Cuts at: 0 872 1407
Size: 872 535
SfcI C'TryA_G
Cuts at: 0 47 1018 1159 1195 1324 1407
Size: 47 971 141 36 129 83
Fragments arranged by size:
971 141 129 83 47 36
SmlI C'TyrA_G
Cuts at: 0 196 1407
Size: 196 1211
SphI G_CATG'C
Cuts at: 0 281 1407
Size: 281 1126
StuI AGG'CCT
Cuts at: 0 839 1407
Size: 839 568
StyI C'CwwG_G
Cuts at: 0 243 358 711 1407
Size: 243 115 353 696
Fragments arranged by size:
696 353 243 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 393 403 1407
Size: 393 10 1004
Fragments arranged by size:
1004 393 10
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI AvaI BaeI BamHI
BanII BclI BlpI BmtI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I FalI HindIII MscI MslI MspA1I NcoI NheI
NspI PasI PflMI PfoI PpuMI PshAI PstI PvuII
SacI SacII SfcI SmlI SphI StuI StyI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BtgZI ClaI CspCI DraI DrdI
EagI EarI EciI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI