Restriction map for
pF1KA1381
(Linear) (Six-base) MAPSORT of: KIAA1381.seq Check: 7120 from: 1 to: 2940
KIAA1381.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 525 977 2940
Size: 525 452 1963
Fragments arranged by size:
1963 525 452
Acc65I G'GTAC_C
Cuts at: 0 164 2940
Size: 164 2776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 817 1594 1903 2940
Size: 49 768 777 309 1037
Fragments arranged by size:
1037 777 768 309 49
AflIII A'CryG_T
Cuts at: 0 1067 1409 2888 2940
Size: 1067 342 1479 52
Fragments arranged by size:
1479 1067 342 52
AhdI GACnn_n'nnGTC
Cuts at: 0 2869 2889 2940
Size: 2869 20 51
Fragments arranged by size:
2869 51 20
AleI CACnn'nnGTG
Cuts at: 0 740 2940
Size: 740 2200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 824 1817 2940
Size: 604 220 993 1123
Fragments arranged by size:
1123 993 604 220
AlwNI CAG_nnn'CTG
Cuts at: 0 1260 1284 1850 1860 2179 2848 2940
Size: 1260 24 566 10 319 669 92
Fragments arranged by size:
1260 669 566 319 92 24 10
ApaI G_GGCC'C
Cuts at: 0 309 2940
Size: 309 2631
ApoI r'AATT_y
Cuts at: 0 377 2159 2940
Size: 377 1782 781
Fragments arranged by size:
1782 781 377
AseI AT'TA_AT
Cuts at: 0 2267 2940
Size: 2267 673
BamHI G'GATC_C
Cuts at: 0 1061 2940
Size: 1061 1879
BanI G'GyrC_C
Cuts at: 0 164 2940
Size: 164 2776
BanII G_rGCy'C
Cuts at: 0 309 400 1331 1915 1944 2940
Size: 309 91 931 584 29 996
Fragments arranged by size:
996 931 584 309 91 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1085 2888 2940
Size: 1085 1803 52
Fragments arranged by size:
1803 1085 52
BfrBI ATG'CAT
Cuts at: 0 1730 2940
Size: 1730 1210
BglI GCCn_nnn'nGGC
Cuts at: 0 10 241 278 283 703 2940
Size: 10 231 37 5 420 2237
Fragments arranged by size:
2237 420 231 37 10 5
BglII A'GATC_T
Cuts at: 0 389 2940
Size: 389 2551
Bme1580I G_kGCm'C
Cuts at: 0 309 922 1876 2940
Size: 309 613 954 1064
Fragments arranged by size:
1064 954 613 309
BmgBI CAC'GTC
Cuts at: 0 2891 2940
Size: 2891 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 2088 2940
Size: 2088 852
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 683 2376 2940
Size: 683 1693 564
Fragments arranged by size:
1693 683 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 414 454 904 1240 1296 2008 2073
Size: 118 296 40 450 336 56 712 65
Cuts at: 2073 2490 2940
Size: 417 450
Fragments arranged by size:
712 450 450 417 336 296 118 65
56 40
Bpu10I CC'TnA_GC
Cuts at: 0 2801 2940
Size: 2801 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 880 1138 2940
Size: 880 258 1802
Fragments arranged by size:
1802 880 258
BsaAI yAC'GTr
Cuts at: 0 1116 2940
Size: 1116 1824
BsaHI Gr'CG_yC
Cuts at: 0 233 1151 2940
Size: 233 918 1789
Fragments arranged by size:
1789 918 233
BsaWI w'CCGG_w
Cuts at: 0 381 557 2940
Size: 381 176 2383
Fragments arranged by size:
2383 381 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1294 1324 2161 2191 2940
Size: 1294 30 837 30 749
Fragments arranged by size:
1294 837 749 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 151 656 2011 2182 2940
Size: 97 54 505 1355 171 758
Fragments arranged by size:
1355 758 505 171 97 54
BseYI C'CCAG_C
Cuts at: 0 308 1193 2144 2940
Size: 308 885 951 796
Fragments arranged by size:
951 885 796 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1717 1807 2081 2198 2626 2879 2940
Size: 1717 90 274 117 428 253 61
Fragments arranged by size:
1717 428 274 253 117 90 61
BsiEI CG_ry'CG
Cuts at: 0 188 287 2940
Size: 188 99 2653
Fragments arranged by size:
2653 188 99
BsiHKAI G_wGCw'C
Cuts at: 0 128 400 640 1331 1915 2940
Size: 128 272 240 691 584 1025
Fragments arranged by size:
1025 691 584 272 240 128
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 2940
Size: 61 2879
Bsp1286I G_dGCh'C
Cuts at: 0 128 309 400 640 922 1331 1876 1915
Size: 128 181 91 240 282 409 545 39
Cuts at: 1915 1944 2940
Size: 29 996
Fragments arranged by size:
996 545 409 282 240 181 128 91
39 29
BspEI T'CCGG_A
Cuts at: 0 381 2940
Size: 381 2559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 525 977 1112 1454 2940
Size: 449 76 452 135 342 1486
Fragments arranged by size:
1486 452 449 342 135 76
BsrBI CCG'CTC
Cuts at: 0 79 1230 2940
Size: 79 1151 1710
Fragments arranged by size:
1710 1151 79
BsrFI r'CCGG_y
Cuts at: 0 277 2940
Size: 277 2663
BsrGI T'GTAC_A
Cuts at: 0 1539 2232 2940
Size: 1539 693 708
Fragments arranged by size:
1539 708 693
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1704 2940
Size: 1704 1236
BstEII G'GTnAC_C
Cuts at: 0 2833 2940
Size: 2833 107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 745 2940
Size: 745 2195
BstYI r'GATC_y
Cuts at: 0 39 86 389 846 1061 2201 2940
Size: 39 47 303 457 215 1140 739
Fragments arranged by size:
1140 739 457 303 215 47 39
BtgI C'CryG_G
Cuts at: 0 8 90 218 301 738 1721 2940
Size: 8 82 128 83 437 983 1219
Fragments arranged by size:
1219 983 437 128 83 82 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2581 2940
Size: 2581 359
BtsI GCAGTG_nn'
Cuts at: 0 1624 1894 2064 2193 2940
Size: 1624 270 170 129 747
Fragments arranged by size:
1624 747 270 170 129
DraIII CAC_nnn'GTG
Cuts at: 0 1743 2940
Size: 1743 1197
EaeI y'GGCC_r
Cuts at: 0 2 185 198 284 295 623 695 785
Size: 2 183 13 86 11 328 72 90
Cuts at: 785 1841 2129 2487 2940
Size: 1056 288 358 453
Fragments arranged by size:
1056 453 358 328 288 183 90 86
72 13 11 2
EagI C'GGCC_G
Cuts at: 0 185 284 2940
Size: 185 99 2656
Fragments arranged by size:
2656 185 99
EarI CTCTTCn'nnn_
Cuts at: 0 650 877 932 1114 1423 1772 2476 2940
Size: 650 227 55 182 309 349 704 464
Fragments arranged by size:
704 650 464 349 309 227 182 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 259 2940
Size: 259 2681
EcoICRI GAG'CTC
Cuts at: 0 398 1329 1913 2940
Size: 398 931 584 1027
Fragments arranged by size:
1027 931 584 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 118 414 454 817 904 1240 1296
Size: 49 69 296 40 363 87 336 56
Cuts at: 1296 1594 1903 2008 2073 2490 2940
Size: 298 309 105 65 417 450
Fragments arranged by size:
450 417 363 336 309 298 296 105
87 69 65 56 49 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 178 271 2940
Size: 178 93 2669
Fragments arranged by size:
2669 178 93
EcoO109I rG'GnC_Cy
Cuts at: 0 630 2940
Size: 630 2310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 1248 1347 2940
Size: 387 861 99 1593
Fragments arranged by size:
1593 861 387 99
HaeII r_GCGC'y
Cuts at: 0 105 2419 2940
Size: 105 2314 521
Fragments arranged by size:
2314 521 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 1606 1638 2104 2136 2161 2193
Size: 1294 32 280 32 466 32 25 32
Cuts at: 2193 2745 2777 2855 2875 2887 2907 2940
Size: 552 32 78 20 12 20 33
Fragments arranged by size:
1294 552 466 280 78 33 32 32
32 32 32 25 20 20 12
HincII GTy'rAC
Cuts at: 0 563 2940
Size: 563 2377
KpnI G_GTAC'C
Cuts at: 0 168 2940
Size: 168 2772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2229 2256 2940
Size: 2229 27 684
Fragments arranged by size:
2229 684 27
MscI TGG'CCA
Cuts at: 0 4 697 787 1843 2131 2940
Size: 4 693 90 1056 288 809
Fragments arranged by size:
1056 809 693 288 90 4
MslI CAynn'nnrTG
Cuts at: 0 740 1114 2940
Size: 740 374 1826
Fragments arranged by size:
1826 740 374
MspA1I CmG'CkG
Cuts at: 0 10 92 212 303 474 956 1193 2144
Size: 10 82 120 91 171 482 237 951
Cuts at: 2144 2424 2830 2940
Size: 280 406 110
Fragments arranged by size:
951 482 406 280 237 171 120 110
91 82 10
NaeI GCC'GGC
Cuts at: 0 279 2940
Size: 279 2661
NcoI C'CATG_G
Cuts at: 0 738 2940
Size: 738 2202
NgoMIV G'CCGG_C
Cuts at: 0 277 2940
Size: 277 2663
NsiI A_TGCA'T
Cuts at: 0 1732 2940
Size: 1732 1208
NspI r_CATG'y
Cuts at: 0 1071 1413 1730 2940
Size: 1071 342 317 1210
Fragments arranged by size:
1210 1071 342 317
PasI CC'CwG_GG
Cuts at: 0 1860 2940
Size: 1860 1080
PciI A'CATG_T
Cuts at: 0 1067 1409 2940
Size: 1067 342 1531
Fragments arranged by size:
1531 1067 342
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 739 1860 2144 2940
Size: 621 118 1121 284 796
Fragments arranged by size:
1121 796 621 284 118
PfoI T'CCnGG_A
Cuts at: 0 1145 2940
Size: 1145 1795
PspOMI G'GGCC_C
Cuts at: 0 305 2940
Size: 305 2635
PstI C_TGCA'G
Cuts at: 0 935 955 1055 2423 2940
Size: 935 20 100 1368 517
Fragments arranged by size:
1368 935 517 100 20
PvuII CAG'CTG
Cuts at: 0 474 956 1193 2144 2424 2830 2940
Size: 474 482 237 951 280 406 110
Fragments arranged by size:
951 482 474 406 280 237 110
SacI G_AGCT'C
Cuts at: 0 400 1331 1915 2940
Size: 400 931 584 1025
Fragments arranged by size:
1025 931 584 400
SacII CC_GC'GG
Cuts at: 0 11 93 304 2940
Size: 11 82 211 2636
Fragments arranged by size:
2636 211 82 11
SapI GCTCTTCn'nnn_
Cuts at: 0 877 1114 1423 2940
Size: 877 237 309 1517
Fragments arranged by size:
1517 877 309 237
SbfI CC_TGCA'GG
Cuts at: 0 935 2940
Size: 935 2005
ScaI AGT'ACT
Cuts at: 0 256 2018 2940
Size: 256 1762 922
Fragments arranged by size:
1762 922 256
SexAI A'CCwGG_T
Cuts at: 0 2045 2940
Size: 2045 895
SfcI C'TryA_G
Cuts at: 0 342 931 951 1051 1765 2419 2940
Size: 342 589 20 100 714 654 521
Fragments arranged by size:
714 654 589 521 342 100 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 283 2940
Size: 283 2657
SphI G_CATG'C
Cuts at: 0 1730 2940
Size: 1730 1210
StyI C'CwwG_G
Cuts at: 0 600 738 1322 1631 2465 2940
Size: 600 138 584 309 834 475
Fragments arranged by size:
834 600 584 475 309 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1286 2107 2419 2940
Size: 1286 821 312 521
Fragments arranged by size:
1286 821 521 312
TatI w'GTAC_w
Cuts at: 0 254 1403 1539 2016 2232 2940
Size: 254 1149 136 477 216 708
Fragments arranged by size:
1149 708 477 254 216 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 2940
Size: 1141 32 1767
Fragments arranged by size:
1767 1141 32
Tth111I GACn'n_nGTC
Cuts at: 0 1716 2940
Size: 1716 1224
XbaI T'CTAG_A
Cuts at: 0 2106 2940
Size: 2106 834
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 736 1329 2940
Size: 736 593 1611
Fragments arranged by size:
1611 736 593
XmnI GAAnn'nnTTC
Cuts at: 0 1979 2940
Size: 1979 961
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AseI BamHI BanI BanII BbsI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PspOMI PstI PvuII SacI SacII
SapI SbfI ScaI SexAI SfcI SfiI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AloI ApaLI
AscI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BlpI BmtI BpuEI BsaBI BsiWI BsmI
BspHI BsrDI BssHII BssSI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI PacI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI XhoI XmaI ZraI