Restriction map for
pF1KA1375
(Linear) (Six-base) MAPSORT of: KIAA1375.seq Check: 5115 from: 1 to: 2604
KIAA1375.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2181 2604
Size: 2181 423
AclI AA'CG_TT
Cuts at: 0 1422 2604
Size: 1422 1182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1467 2604
Size: 1467 1137
AflII C'TTAA_G
Cuts at: 0 510 1941 2604
Size: 510 1431 663
Fragments arranged by size:
1431 663 510
AhdI GACnn_n'nnGTC
Cuts at: 0 560 2604
Size: 560 2044
AleI CACnn'nnGTG
Cuts at: 0 1009 1558 2604
Size: 1009 549 1046
Fragments arranged by size:
1046 1009 549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 779 2604
Size: 779 1825
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 227 259 2604
Size: 227 32 2345
Fragments arranged by size:
2345 227 32
AlwNI CAG_nnn'CTG
Cuts at: 0 417 547 1582 2604
Size: 417 130 1035 1022
Fragments arranged by size:
1035 1022 417 130
ApoI r'AATT_y
Cuts at: 0 241 805 1048 1411 1728 1903 1962 2604
Size: 241 564 243 363 317 175 59 642
Fragments arranged by size:
642 564 363 317 243 241 175 59
AvaI C'yCGr_G
Cuts at: 0 193 2538 2604
Size: 193 2345 66
Fragments arranged by size:
2345 193 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2161 2194 2491 2524 2604
Size: 2161 33 297 33 80
Fragments arranged by size:
2161 297 80 33 33
BanI G'GyrC_C
Cuts at: 0 472 2604
Size: 472 2132
BanII G_rGCy'C
Cuts at: 0 137 2304 2604
Size: 137 2167 300
Fragments arranged by size:
2167 300 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 2101 2604
Size: 2101 503
BbvCI CC'TCA_GC
Cuts at: 0 2570 2604
Size: 2570 34
BclI T'GATC_A
Cuts at: 0 216 1299 2604
Size: 216 1083 1305
Fragments arranged by size:
1305 1083 216
BglII A'GATC_T
Cuts at: 0 263 981 1264 2604
Size: 263 718 283 1340
Fragments arranged by size:
1340 718 283 263
BlpI GC'TnA_GC
Cuts at: 0 136 2373 2604
Size: 136 2237 231
Fragments arranged by size:
2237 231 136
Bme1580I G_kGCm'C
Cuts at: 0 921 2604
Size: 921 1683
BmrI ACTGGGnnnn_n'
Cuts at: 0 105 2604
Size: 105 2499
BmtI G_CTAG'C
Cuts at: 0 395 1189 2604
Size: 395 794 1415
Fragments arranged by size:
1415 794 395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2128 2604
Size: 2128 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1247 1726 2207 2309 2604
Size: 524 723 479 481 102 295
Fragments arranged by size:
723 524 481 479 295 102
Bpu10I CC'TnA_GC
Cuts at: 0 345 387 1583 2570 2604
Size: 345 42 1196 987 34
Fragments arranged by size:
1196 987 345 42 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1751 2604
Size: 569 1182 853
Fragments arranged by size:
1182 853 569
BsaI GGTCTCn'nnnn_
Cuts at: 0 1896 2604
Size: 1896 708
BsaBI GATnn'nnATC
Cuts at: 0 986 2604
Size: 986 1618
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 1220 1250 2604
Size: 308 30 882 30 1354
Fragments arranged by size:
1354 882 308 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 2204 2369 2372 2604
Size: 145 2059 165 3 232
Fragments arranged by size:
2059 232 165 145 3
BseYI C'CCAG_C
Cuts at: 0 2255 2262 2335 2546 2604
Size: 2255 7 73 211 58
Fragments arranged by size:
2255 211 73 58 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 401 1528 2604
Size: 40 361 1127 1076
Fragments arranged by size:
1127 1076 361 40
BsiEI CG_ry'CG
Cuts at: 0 165 527 2604
Size: 165 362 2077
Fragments arranged by size:
2077 362 165
BsiHKAI G_wGCw'C
Cuts at: 0 137 2157 2304 2604
Size: 137 2020 147 300
Fragments arranged by size:
2020 300 147 137
BsmI GAATG_Cn'
Cuts at: 0 1596 2604
Size: 1596 1008
BsmBI CGTCTCn'nnnn_
Cuts at: 0 190 487 2604
Size: 190 297 2117
Fragments arranged by size:
2117 297 190
Bsp1286I G_dGCh'C
Cuts at: 0 137 921 2157 2304 2604
Size: 137 784 1236 147 300
Fragments arranged by size:
1236 784 300 147 137
BspHI T'CATG_A
Cuts at: 0 957 2604
Size: 957 1647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2181 2604
Size: 2181 423
BsrDI GCAATG_nn'
Cuts at: 0 2135 2604
Size: 2135 469
BssHII G'CGCG_C
Cuts at: 0 188 2604
Size: 188 2416
BssSI C'ACGA_G
Cuts at: 0 181 2604
Size: 181 2423
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 1170 2454 2521 2604
Size: 60 1110 1284 67 83
Fragments arranged by size:
1284 1110 83 67 60
BstYI r'GATC_y
Cuts at: 0 263 981 987 1264 2604
Size: 263 718 6 277 1340
Fragments arranged by size:
1340 718 277 263 6
BtgI C'CryG_G
Cuts at: 0 527 2453 2604
Size: 527 1926 151
Fragments arranged by size:
1926 527 151
BtsI GCAGTG_nn'
Cuts at: 0 1641 2079 2604
Size: 1641 438 525
Fragments arranged by size:
1641 525 438
DraI TTT'AAA
Cuts at: 0 602 2604
Size: 602 2002
EaeI y'GGCC_r
Cuts at: 0 50 2478 2604
Size: 50 2428 126
Fragments arranged by size:
2428 126 50
EarI CTCTTCn'nnn_
Cuts at: 0 95 2604
Size: 95 2509
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 2604
Size: 142 2462
EcoICRI GAG'CTC
Cuts at: 0 135 2302 2604
Size: 135 2167 302
Fragments arranged by size:
2167 302 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1247 1467 1726 2207 2309 2604
Size: 524 723 220 259 481 102 295
Fragments arranged by size:
723 524 481 295 259 220 102
EcoO109I rG'GnC_Cy
Cuts at: 0 554 2542 2604
Size: 554 1988 62
Fragments arranged by size:
1988 554 62
EcoRV GAT'ATC
Cuts at: 0 2425 2604
Size: 2425 179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 325 1024 1810 2604
Size: 325 699 786 794
Fragments arranged by size:
794 786 699 325
HaeII r_GCGC'y
Cuts at: 0 2230 2604
Size: 2230 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 546 578 1284 1316 1705 1737
Size: 425 32 89 32 706 32 389 32
Cuts at: 1737 2604
Size: 867
Fragments arranged by size:
867 706 425 389 89 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1134 2039 2604
Size: 1134 905 565
Fragments arranged by size:
1134 905 565
HindIII A'AGCT_T
Cuts at: 0 342 2604
Size: 342 2262
HpaI GTT'AAC
Cuts at: 0 1134 2604
Size: 1134 1470
MfeI C'AATT_G
Cuts at: 0 375 1142 1211 2604
Size: 375 767 69 1393
Fragments arranged by size:
1393 767 375 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 2242 2604
Size: 1352 890 362
Fragments arranged by size:
1352 890 362
MscI TGG'CCA
Cuts at: 0 52 2480 2604
Size: 52 2428 124
Fragments arranged by size:
2428 124 52
MslI CAynn'nnrTG
Cuts at: 0 16 1009 1558 2452 2604
Size: 16 993 549 894 152
Fragments arranged by size:
993 894 549 152 16
MspA1I CmG'CkG
Cuts at: 0 24 60 171 2604
Size: 24 36 111 2433
Fragments arranged by size:
2433 111 36 24
NcoI C'CATG_G
Cuts at: 0 2453 2604
Size: 2453 151
NdeI CA'TA_TG
Cuts at: 0 1977 2604
Size: 1977 627
NheI G'CTAG_C
Cuts at: 0 391 1185 2604
Size: 391 794 1419
Fragments arranged by size:
1419 794 391
NspI r_CATG'y
Cuts at: 0 838 2206 2604
Size: 838 1368 398
Fragments arranged by size:
1368 838 398
PfoI T'CCnGG_A
Cuts at: 0 2418 2520 2604
Size: 2418 102 84
Fragments arranged by size:
2418 102 84
PpuMI rG'GwC_Cy
Cuts at: 0 554 2542 2604
Size: 554 1988 62
Fragments arranged by size:
1988 554 62
PstI C_TGCA'G
Cuts at: 0 663 930 2604
Size: 663 267 1674
Fragments arranged by size:
1674 663 267
PvuII CAG'CTG
Cuts at: 0 24 2604
Size: 24 2580
SacI G_AGCT'C
Cuts at: 0 137 2304 2604
Size: 137 2167 300
Fragments arranged by size:
2167 300 137
SanDI GG'GwC_CC
Cuts at: 0 554 2542 2604
Size: 554 1988 62
Fragments arranged by size:
1988 554 62
SapI GCTCTTCn'nnn_
Cuts at: 0 95 2604
Size: 95 2509
ScaI AGT'ACT
Cuts at: 0 115 2604
Size: 115 2489
SfcI C'TryA_G
Cuts at: 0 659 926 2604
Size: 659 267 1678
Fragments arranged by size:
1678 659 267
SmaI CCC'GGG
Cuts at: 0 2540 2604
Size: 2540 64
SmlI C'TyrA_G
Cuts at: 0 193 510 584 1766 1941 2604
Size: 193 317 74 1182 175 663
Fragments arranged by size:
1182 663 317 193 175 74
SphI G_CATG'C
Cuts at: 0 838 2604
Size: 838 1766
SspI AAT'ATT
Cuts at: 0 1884 2604
Size: 1884 720
StuI AGG'CCT
Cuts at: 0 2271 2604
Size: 2271 333
StyI C'CwwG_G
Cuts at: 0 713 2453 2604
Size: 713 1740 151
Fragments arranged by size:
1740 713 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 2604
Size: 151 2453
TatI w'GTAC_w
Cuts at: 0 113 1086 2604
Size: 113 973 1518
Fragments arranged by size:
1518 973 113
XbaI T'CTAG_A
Cuts at: 0 984 2604
Size: 984 1620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 466 2604
Size: 466 2138
XhoI C'TCGA_G
Cuts at: 0 193 2604
Size: 193 2411
XmaI C'CCGG_G
Cuts at: 0 2538 2604
Size: 2538 66
Enzymes that do cut:
AarI AclI AcuI AflII AhdI AleI AlfI AloI
AlwNI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BssHII BssSI BstXI BstYI BtgI BtsI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NheI NspI PfoI PpuMI PstI
PvuII SacI SanDI SapI ScaI SfcI SmaI SmlI
SphI SspI StuI StyI TaqII TatI XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AgeI ApaI ApaLI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI
BfrBI BglI BmgBI BsaAI BsaHI BsaWI BsiWI BspEI
BsrBI BsrFI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EcoNI EcoRI
FseI FspI FspAI KasI KpnI MluI NaeI NarI
NgoMIV NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SwaI TstI Tth111I
XmnI ZraI