Restriction map for
pF1KA1370
(Linear) (Six-base) MAPSORT of: KIAA1370.seq Check: 4951 from: 1 to: 3228
KIAA1370.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 1182 3228
Size: 1182 2046
AccI GT'mk_AC
Cuts at: 0 548 2311 3228
Size: 548 1763 917
Fragments arranged by size:
1763 917 548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2002 2843 3228
Size: 2002 841 385
Fragments arranged by size:
2002 841 385
AflIII A'CryG_T
Cuts at: 0 1167 2137 2314 3228
Size: 1167 970 177 914
Fragments arranged by size:
1167 970 914 177
AhdI GACnn_n'nnGTC
Cuts at: 0 2307 3228
Size: 2307 921
AlwNI CAG_nnn'CTG
Cuts at: 0 798 910 3228
Size: 798 112 2318
Fragments arranged by size:
2318 798 112
ApaI G_GGCC'C
Cuts at: 0 314 3228
Size: 314 2914
ApoI r'AATT_y
Cuts at: 0 561 716 1331 1584 2002 2640 3228
Size: 561 155 615 253 418 638 588
Fragments arranged by size:
638 615 588 561 418 253 155
AvrII C'CTAG_G
Cuts at: 0 2634 3228
Size: 2634 594
BanI G'GyrC_C
Cuts at: 0 825 3228
Size: 825 2403
BanII G_rGCy'C
Cuts at: 0 314 3228
Size: 314 2914
BbsI GAAGACnn'nnnn_
Cuts at: 0 436 1319 1583 3228
Size: 436 883 264 1645
Fragments arranged by size:
1645 883 436 264
BfrBI ATG'CAT
Cuts at: 0 361 1530 3228
Size: 361 1169 1698
Fragments arranged by size:
1698 1169 361
BglII A'GATC_T
Cuts at: 0 3101 3228
Size: 3101 127
BlpI GC'TnA_GC
Cuts at: 0 1469 3228
Size: 1469 1759
Bme1580I G_kGCm'C
Cuts at: 0 314 3228
Size: 314 2914
BmgBI CAC'GTC
Cuts at: 0 677 3228
Size: 677 2551
BmrI ACTGGGnnnn_n'
Cuts at: 0 154 192 3228
Size: 154 38 3036
Fragments arranged by size:
3036 154 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 512 3228
Size: 512 2716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1420 1521 3228
Size: 125 1295 101 1707
Fragments arranged by size:
1707 1295 125 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1178 3228
Size: 923 255 2050
Fragments arranged by size:
2050 923 255
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 3228
Size: 66 3162
BsaAI yAC'GTr
Cuts at: 0 1790 3228
Size: 1790 1438
BsaHI Gr'CG_yC
Cuts at: 0 1179 3228
Size: 1179 2049
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 1343 3228
Size: 64 1279 1885
Fragments arranged by size:
1885 1279 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 3228
Size: 699 2529
BsiHKAI G_wGCw'C
Cuts at: 0 1484 2610 3228
Size: 1484 1126 618
Fragments arranged by size:
1484 1126 618
BsiWI C'GTAC_G
Cuts at: 0 3120 3228
Size: 3120 108
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3110 3228
Size: 3110 118
Bsp1286I G_dGCh'C
Cuts at: 0 314 1484 2610 3228
Size: 314 1170 1126 618
Fragments arranged by size:
1170 1126 618 314
BspHI T'CATG_A
Cuts at: 0 2580 3228
Size: 2580 648
BsrBI CCG'CTC
Cuts at: 0 452 1107 1443 3228
Size: 452 655 336 1785
Fragments arranged by size:
1785 655 452 336
BsrDI GCAATG_nn'
Cuts at: 0 132 1118 1908 3228
Size: 132 986 790 1320
Fragments arranged by size:
1320 986 790 132
BsrGI T'GTAC_A
Cuts at: 0 1954 3228
Size: 1954 1274
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1129 1465 2722 3228
Size: 1129 336 1257 506
Fragments arranged by size:
1257 1129 506 336
BstEII G'GTnAC_C
Cuts at: 0 409 3228
Size: 409 2819
BstYI r'GATC_y
Cuts at: 0 380 2250 3101 3228
Size: 380 1870 851 127
Fragments arranged by size:
1870 851 380 127
BtgI C'CryG_G
Cuts at: 0 821 927 3228
Size: 821 106 2301
Fragments arranged by size:
2301 821 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 317 3228
Size: 317 2911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 248 283 3228
Size: 248 35 2945
Fragments arranged by size:
2945 248 35
DraI TTT'AAA
Cuts at: 0 1007 1704 3228
Size: 1007 697 1524
Fragments arranged by size:
1524 1007 697
EarI CTCTTCn'nnn_
Cuts at: 0 419 2450 2753 3001 3228
Size: 419 2031 303 248 227
Fragments arranged by size:
2031 419 303 248 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1420 1521 2002 2843 3228
Size: 125 1295 101 481 841 385
Fragments arranged by size:
1295 841 481 385 125 101
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1085 1421 2858 3228
Size: 1085 336 1437 370
Fragments arranged by size:
1437 1085 370 336
EcoRI G'AATT_C
Cuts at: 0 716 3228
Size: 716 2512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1382 2741 3228
Size: 1382 1359 487
Fragments arranged by size:
1382 1359 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 964 996 1597 1629 2293 2325 3228
Size: 964 32 601 32 664 32 903
Fragments arranged by size:
964 903 664 601 32 32 32
HindIII A'AGCT_T
Cuts at: 0 126 1268 2523 3228
Size: 126 1142 1255 705
Fragments arranged by size:
1255 1142 705 126
MfeI C'AATT_G
Cuts at: 0 578 3228
Size: 578 2650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1383 1516 2048 2278 3057 3228
Size: 1383 133 532 230 779 171
Fragments arranged by size:
1383 779 532 230 171 133
MslI CAynn'nnrTG
Cuts at: 0 99 240 2366 3222 3228
Size: 99 141 2126 856 6
Fragments arranged by size:
2126 856 141 99 6
MspA1I CmG'CkG
Cuts at: 0 880 1066 3228
Size: 880 186 2162
Fragments arranged by size:
2162 880 186
NcoI C'CATG_G
Cuts at: 0 821 927 3228
Size: 821 106 2301
Fragments arranged by size:
2301 821 106
NdeI CA'TA_TG
Cuts at: 0 1394 1572 3171 3228
Size: 1394 178 1599 57
Fragments arranged by size:
1599 1394 178 57
NsiI A_TGCA'T
Cuts at: 0 363 1532 3228
Size: 363 1169 1696
Fragments arranged by size:
1696 1169 363
NspI r_CATG'y
Cuts at: 0 589 847 1171 1530 2106 2141 2318 3228
Size: 589 258 324 359 576 35 177 910
Fragments arranged by size:
910 589 576 359 324 258 177 35
PciI A'CATG_T
Cuts at: 0 1167 2137 2314 3228
Size: 1167 970 177 914
Fragments arranged by size:
1167 970 914 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 910 3228
Size: 910 2318
PfoI T'CCnGG_A
Cuts at: 0 1436 3228
Size: 1436 1792
PspOMI G'GGCC_C
Cuts at: 0 310 3228
Size: 310 2918
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 771 803 3038 3070 3228
Size: 771 32 2235 32 158
Fragments arranged by size:
2235 771 158 32 32
PstI C_TGCA'G
Cuts at: 0 879 3228
Size: 879 2349
PvuII CAG'CTG
Cuts at: 0 880 1066 3228
Size: 880 186 2162
Fragments arranged by size:
2162 880 186
SfcI C'TryA_G
Cuts at: 0 297 525 553 875 2769 3228
Size: 297 228 28 322 1894 459
Fragments arranged by size:
1894 459 322 297 228 28
SmlI C'TyrA_G
Cuts at: 0 938 1193 3228
Size: 938 255 2035
Fragments arranged by size:
2035 938 255
SnaBI TAC'GTA
Cuts at: 0 1790 3228
Size: 1790 1438
SspI AAT'ATT
Cuts at: 0 31 519 696 948 2083 3228
Size: 31 488 177 252 1135 1145
Fragments arranged by size:
1145 1135 488 252 177 31
StuI AGG'CCT
Cuts at: 0 2130 3228
Size: 2130 1098
StyI C'CwwG_G
Cuts at: 0 821 927 2396 2634 2829 3228
Size: 821 106 1469 238 195 399
Fragments arranged by size:
1469 821 399 238 195 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 26 428 2797 3228
Size: 26 402 2369 431
Fragments arranged by size:
2369 431 402 26
TatI w'GTAC_w
Cuts at: 0 1954 3228
Size: 1954 1274
XbaI T'CTAG_A
Cuts at: 0 2533 3136 3228
Size: 2533 603 92
Fragments arranged by size:
2533 603 92
XmnI GAAnn'nnTTC
Cuts at: 0 128 2524 2979 3228
Size: 128 2396 455 249
Fragments arranged by size:
2396 455 249 128
ZraI GAC'GTC
Cuts at: 0 1180 3228
Size: 1180 2048
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlwNI ApaI ApoI
AvrII BanI BanII BbsI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI
BseRI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BsrBI
BsrDI BsrGI BstAPI BstEII BstYI BtgI BtgZI CspCI
DraI EarI Eco57MI EcoNI EcoRI FalI Hin4I HindIII
MfeI MmeI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PspOMI PsrI PstI PvuII SfcI
SmlI SnaBI SspI StuI StyI TaqII TatI XbaI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvaI BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BglI BmtI Bpu10I
BsaBI BsaWI BsaXI BseYI BsiEI BsmI BspEI BspMI
BsrFI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtsI
ClaI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI SwaI TstI Tth111I XcmI
XhoI XmaI