Restriction map for
pF1KA1364
(Linear) (Six-base) MAPSORT of: KIAA1364.seq Check: 186 from: 1 to: 2844
KIAA1364.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 79 2844
Size: 79 2765
AatII G_ACGT'C
Cuts at: 0 2792 2844
Size: 2792 52
AccI GT'mk_AC
Cuts at: 0 2312 2844
Size: 2312 532
Acc65I G'GTAC_C
Cuts at: 0 2724 2844
Size: 2724 120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1362 2844
Size: 1362 1482
AgeI A'CCGG_T
Cuts at: 0 50 2844
Size: 50 2794
AlwNI CAG_nnn'CTG
Cuts at: 0 831 1050 2187 2271 2844
Size: 831 219 1137 84 573
Fragments arranged by size:
1137 831 573 219 84
ApaLI G'TGCA_C
Cuts at: 0 249 610 2844
Size: 249 361 2234
Fragments arranged by size:
2234 361 249
ApoI r'AATT_y
Cuts at: 0 544 643 706 741 817 1512 2194 2431
Size: 544 99 63 35 76 695 682 237
Cuts at: 2431 2844
Size: 413
Fragments arranged by size:
695 682 544 413 237 99 76 63
35
AvaI C'yCGr_G
Cuts at: 0 977 1235 2078 2844
Size: 977 258 843 766
Fragments arranged by size:
977 843 766 258
AvrII C'CTAG_G
Cuts at: 0 516 2844
Size: 516 2328
BanI G'GyrC_C
Cuts at: 0 421 1580 2542 2656 2724 2776 2822 2844
Size: 421 1159 962 114 68 52 46 22
Fragments arranged by size:
1159 962 421 114 68 52 46 22
BanII G_rGCy'C
Cuts at: 0 104 968 1300 2245 2633 2844
Size: 104 864 332 945 388 211
Fragments arranged by size:
945 864 388 332 211 104
BbeI G_GCGC'C
Cuts at: 0 2546 2780 2826 2844
Size: 2546 234 46 18
Fragments arranged by size:
2546 234 46 18
BbvCI CC'TCA_GC
Cuts at: 0 2708 2844
Size: 2708 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2166 2200 2844
Size: 2166 34 644
Fragments arranged by size:
2166 644 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 484 1421 2844
Size: 484 937 1423
Fragments arranged by size:
1423 937 484
BclI T'GATC_A
Cuts at: 0 653 2844
Size: 653 2191
BglI GCCn_nnn'nGGC
Cuts at: 0 196 736 1021 1212 2844
Size: 196 540 285 191 1632
Fragments arranged by size:
1632 540 285 196 191
BglII A'GATC_T
Cuts at: 0 1623 2844
Size: 1623 1221
BlpI GC'TnA_GC
Cuts at: 0 1006 2844
Size: 1006 1838
Bme1580I G_kGCm'C
Cuts at: 0 253 606 614 2659 2844
Size: 253 353 8 2045 185
Fragments arranged by size:
2045 353 253 185 8
BmgBI CAC'GTC
Cuts at: 0 534 2844
Size: 534 2310
BmrI ACTGGGnnnn_n'
Cuts at: 0 331 2844
Size: 331 2513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 2644 2698 2844
Size: 473 2171 54 146
Fragments arranged by size:
2171 473 146 54
Bpu10I CC'TnA_GC
Cuts at: 0 1960 2708 2755 2844
Size: 1960 748 47 89
Fragments arranged by size:
1960 748 89 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 142 2844
Size: 70 72 2702
Fragments arranged by size:
2702 72 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 298 1289 2481 2844
Size: 13 285 991 1192 363
Fragments arranged by size:
1192 991 363 285 13
BsaAI yAC'GTr
Cuts at: 0 2316 2844
Size: 2316 528
BsaHI Gr'CG_yC
Cuts at: 0 2543 2777 2789 2823 2844
Size: 2543 234 12 34 21
Fragments arranged by size:
2543 234 34 21 12
BsaWI w'CCGG_w
Cuts at: 0 50 2354 2727 2844
Size: 50 2304 373 117
Fragments arranged by size:
2304 373 117 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 2844
Size: 124 30 2690
Fragments arranged by size:
2690 124 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1927 2055 2066 2077 2080 2105 2641
Size: 19 1908 128 11 11 3 25 536
Cuts at: 2641 2746 2797 2844
Size: 105 51 47
Fragments arranged by size:
1908 536 128 105 51 47 25 19
11 11 3
BseYI C'CCAG_C
Cuts at: 0 30 1040 1572 2032 2180 2632 2844
Size: 30 1010 532 460 148 452 212
Fragments arranged by size:
1010 532 460 452 212 148 30
BsiHKAI G_wGCw'C
Cuts at: 0 104 253 614 968 2116 2750 2844
Size: 104 149 361 354 1148 634 94
Fragments arranged by size:
1148 634 361 354 149 104 94
BsmI GAATG_Cn'
Cuts at: 0 2211 2844
Size: 2211 633
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1061 2844
Size: 1061 1783
Bsp1286I G_dGCh'C
Cuts at: 0 104 253 606 614 968 1300 2116 2245
Size: 104 149 353 8 354 332 816 129
Cuts at: 2245 2633 2659 2750 2844
Size: 388 26 91 94
Fragments arranged by size:
816 388 354 353 332 149 129 104
94 91 26 8
BspHI T'CATG_A
Cuts at: 0 1784 2844
Size: 1784 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 2789 2844
Size: 79 2710 55
Fragments arranged by size:
2710 79 55
BsrBI CCG'CTC
Cuts at: 0 140 2667 2844
Size: 140 2527 177
Fragments arranged by size:
2527 177 140
BsrDI GCAATG_nn'
Cuts at: 0 1548 1751 2844
Size: 1548 203 1093
Fragments arranged by size:
1548 1093 203
BsrFI r'CCGG_y
Cuts at: 0 50 2490 2844
Size: 50 2440 354
Fragments arranged by size:
2440 354 50
BstAPI GCAn_nnn'nTGC
Cuts at: 0 921 1050 2844
Size: 921 129 1794
Fragments arranged by size:
1794 921 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2577 2844
Size: 2577 267
BstYI r'GATC_y
Cuts at: 0 1623 1942 2844
Size: 1623 319 902
Fragments arranged by size:
1623 902 319
Bsu36I CC'TnA_GG
Cuts at: 0 569 2698 2844
Size: 569 2129 146
Fragments arranged by size:
2129 569 146
BtgI C'CryG_G
Cuts at: 0 711 1808 1832 2576 2844
Size: 711 1097 24 744 268
Fragments arranged by size:
1097 744 711 268 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 711 2622 2844
Size: 711 1911 222
Fragments arranged by size:
1911 711 222
BtsI GCAGTG_nn'
Cuts at: 0 2501 2713 2844
Size: 2501 212 131
Fragments arranged by size:
2501 212 131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 831 866 2844
Size: 831 35 1978
Fragments arranged by size:
1978 831 35
DraIII CAC_nnn'GTG
Cuts at: 0 263 2844
Size: 263 2581
DrdI GACnn_nn'nnGTC
Cuts at: 0 55 2844
Size: 55 2789
EaeI y'GGCC_r
Cuts at: 0 388 722 1097 1213 1438 1971 2189 2579
Size: 388 334 375 116 225 533 218 390
Cuts at: 2579 2844
Size: 265
Fragments arranged by size:
533 390 388 375 334 265 225 218
116
EarI CTCTTCn'nnn_
Cuts at: 0 776 2032 2195 2479 2844
Size: 776 1256 163 284 365
Fragments arranged by size:
1256 776 365 284 163
EcoICRI GAG'CTC
Cuts at: 0 102 966 2844
Size: 102 864 1878
Fragments arranged by size:
1878 864 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1362 2644 2698 2844
Size: 473 889 1282 54 146
Fragments arranged by size:
1282 889 473 146 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2701 2844
Size: 2701 143
EcoO109I rG'GnC_Cy
Cuts at: 0 2527 2844
Size: 2527 317
EcoRI G'AATT_C
Cuts at: 0 544 706 1512 2844
Size: 544 162 806 1332
Fragments arranged by size:
1332 806 544 162
HaeII r_GCGC'y
Cuts at: 0 2546 2780 2826 2844
Size: 2546 234 46 18
Fragments arranged by size:
2546 234 46 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 346 378 1813 1845 1934 1966
Size: 124 32 190 32 1435 32 89 32
Cuts at: 1966 1968 2000 2844
Size: 2 32 844
Fragments arranged by size:
1435 844 190 124 89 32 32 32
32 32 2
HincII GTy'rAC
Cuts at: 0 1428 2844
Size: 1428 1416
HindIII A'AGCT_T
Cuts at: 0 166 2844
Size: 166 2678
KasI G'GCGC_C
Cuts at: 0 2542 2776 2822 2844
Size: 2542 234 46 22
Fragments arranged by size:
2542 234 46 22
KpnI G_GTAC'C
Cuts at: 0 2728 2844
Size: 2728 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 652 679 2844
Size: 510 142 27 2165
Fragments arranged by size:
2165 510 142 27
MscI TGG'CCA
Cuts at: 0 390 724 1099 1215 1973 2191 2581 2844
Size: 390 334 375 116 758 218 390 263
Fragments arranged by size:
758 390 390 375 334 263 218 116
MslI CAynn'nnrTG
Cuts at: 0 536 1117 1460 1837 2844
Size: 536 581 343 377 1007
Fragments arranged by size:
1007 581 536 377 343
MspA1I CmG'CkG
Cuts at: 0 1050 2184 2844
Size: 1050 1134 660
Fragments arranged by size:
1134 1050 660
NarI GG'CG_CC
Cuts at: 0 2543 2777 2823 2844
Size: 2543 234 46 21
Fragments arranged by size:
2543 234 46 21
NcoI C'CATG_G
Cuts at: 0 1832 2844
Size: 1832 1012
NspI r_CATG'y
Cuts at: 0 2291 2844
Size: 2291 553
PflMI CCAn_nnn'nTGG
Cuts at: 0 1637 2187 2844
Size: 1637 550 657
Fragments arranged by size:
1637 657 550
PfoI T'CCnGG_A
Cuts at: 0 95 824 1636 2844
Size: 95 729 812 1208
Fragments arranged by size:
1208 812 729 95
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2329 2361 2844
Size: 2329 32 483
Fragments arranged by size:
2329 483 32
PpuMI rG'GwC_Cy
Cuts at: 0 2527 2844
Size: 2527 317
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 853 885 2844
Size: 853 32 1959
Fragments arranged by size:
1959 853 32
PstI C_TGCA'G
Cuts at: 0 2537 2720 2820 2844
Size: 2537 183 100 24
Fragments arranged by size:
2537 183 100 24
PvuII CAG'CTG
Cuts at: 0 1050 2184 2844
Size: 1050 1134 660
Fragments arranged by size:
1134 1050 660
SacI G_AGCT'C
Cuts at: 0 104 968 2844
Size: 104 864 1876
Fragments arranged by size:
1876 864 104
SbfI CC_TGCA'GG
Cuts at: 0 2537 2844
Size: 2537 307
ScaI AGT'ACT
Cuts at: 0 2377 2844
Size: 2377 467
SfcI C'TryA_G
Cuts at: 0 1550 2533 2716 2816 2844
Size: 1550 983 183 100 28
Fragments arranged by size:
1550 983 183 100 28
SfoI GGC'GCC
Cuts at: 0 2544 2778 2824 2844
Size: 2544 234 46 20
Fragments arranged by size:
2544 234 46 20
SmaI CCC'GGG
Cuts at: 0 1237 2844
Size: 1237 1607
SmlI C'TyrA_G
Cuts at: 0 85 157 2844
Size: 85 72 2687
Fragments arranged by size:
2687 85 72
SpeI A'CTAG_T
Cuts at: 0 1173 2844
Size: 1173 1671
StyI C'CwwG_G
Cuts at: 0 329 516 549 683 1142 1832 1895 2000
Size: 329 187 33 134 459 690 63 105
Cuts at: 2000 2844
Size: 844
Fragments arranged by size:
844 690 459 329 187 134 105 63
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 409 2115 2844
Size: 409 1706 729
Fragments arranged by size:
1706 729 409
TatI w'GTAC_w
Cuts at: 0 1385 2168 2375 2844
Size: 1385 783 207 469
Fragments arranged by size:
1385 783 469 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1395 1427 2844
Size: 1395 32 1417
Fragments arranged by size:
1417 1395 32
Tth111I GACn'n_nGTC
Cuts at: 0 1399 2844
Size: 1399 1445
XmaI C'CCGG_G
Cuts at: 0 1235 2844
Size: 1235 1609
XmnI GAAnn'nnTTC
Cuts at: 0 692 706 2257 2844
Size: 692 14 1551 587
Fragments arranged by size:
1551 692 587 14
ZraI GAC'GTC
Cuts at: 0 2790 2844
Size: 2790 54
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AgeI AlwNI ApaLI
ApoI AvaI AvrII BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NarI NcoI
NspI PflMI PfoI PpiI PpuMI PsrI PstI PvuII
SacI SbfI ScaI SfcI SfoI SmaI SmlI SpeI
StyI TaqII TatI TstI Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AhdI AleI AlfI AloI
ApaI AscI AseI AsiSI BaeI BamHI BbsI BfrBI
BmtI BplI BsaBI BsgI BsiEI BsiWI BspEI BsrGI
BssHII BssSI BstBI BstEII BstZ17I ClaI DraI EagI
EciI EcoRV FalI FseI FspI FspAI HpaI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SexAI
SfiI SgfI SgrAI SnaBI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI