Restriction map for
pF1KA1352
(Linear) (Six-base) MAPSORT of: KIAA1352.seq Check: 6037 from: 1 to: 3528
KIAA1352.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1754 3528
Size: 1754 1774
AccI GT'mk_AC
Cuts at: 0 596 709 3528
Size: 596 113 2819
Fragments arranged by size:
2819 596 113
Acc65I G'GTAC_C
Cuts at: 0 224 3528
Size: 224 3304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 2965 3528
Size: 545 2420 563
Fragments arranged by size:
2420 563 545
AflIII A'CryG_T
Cuts at: 0 188 3528
Size: 188 3340
AleI CACnn'nnGTG
Cuts at: 0 1159 2595 3528
Size: 1159 1436 933
Fragments arranged by size:
1436 1159 933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 3528
Size: 1762 1766
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1442 1474 2528 2560 3528
Size: 1442 32 1054 32 968
Fragments arranged by size:
1442 1054 968 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1767 2668 3528
Size: 1767 901 860
Fragments arranged by size:
1767 901 860
ApoI r'AATT_y
Cuts at: 0 517 694 1067 1349 1677 1987 2197 3037
Size: 517 177 373 282 328 310 210 840
Cuts at: 3037 3464 3528
Size: 427 64
Fragments arranged by size:
840 517 427 373 328 310 282 210
177 64
AseI AT'TA_AT
Cuts at: 0 1054 3528
Size: 1054 2474
BanI G'GyrC_C
Cuts at: 0 224 1035 1284 3528
Size: 224 811 249 2244
Fragments arranged by size:
2244 811 249 224
BbsI GAAGACnn'nnnn_
Cuts at: 0 1480 2776 3155 3418 3528
Size: 1480 1296 379 263 110
Fragments arranged by size:
1480 1296 379 263 110
BciVI GTATCCnnnnn_n'
Cuts at: 0 66 2006 3528
Size: 66 1940 1522
Fragments arranged by size:
1940 1522 66
BfrBI ATG'CAT
Cuts at: 0 100 3528
Size: 100 3428
BmrI ACTGGGnnnn_n'
Cuts at: 0 2349 3528
Size: 2349 1179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1523 3019 3528
Size: 785 738 1496 509
Fragments arranged by size:
1496 785 738 509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1704 2722 3112 3528
Size: 835 869 1018 390 416
Fragments arranged by size:
1018 869 835 416 390
BsaI GGTCTCn'nnnn_
Cuts at: 0 883 1660 3528
Size: 883 777 1868
Fragments arranged by size:
1868 883 777
BsaAI yAC'GTr
Cuts at: 0 2866 3528
Size: 2866 662
BsaBI GATnn'nnATC
Cuts at: 0 629 3528
Size: 629 2899
BsaHI Gr'CG_yC
Cuts at: 0 171 603 3528
Size: 171 432 2925
Fragments arranged by size:
2925 432 171
BsaWI w'CCGG_w
Cuts at: 0 2546 3528
Size: 2546 982
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 758 788 2114 2144 3528
Size: 758 30 1326 30 1384
Fragments arranged by size:
1384 1326 758 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1678 2119 2563 2682 3528
Size: 1678 441 444 119 846
Fragments arranged by size:
1678 846 444 441 119
BseYI C'CCAG_C
Cuts at: 0 1866 3221 3528
Size: 1866 1355 307
Fragments arranged by size:
1866 1355 307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1073 3528
Size: 253 820 2455
Fragments arranged by size:
2455 820 253
BsiHKAI G_wGCw'C
Cuts at: 0 2606 3528
Size: 2606 922
BsmI GAATG_Cn'
Cuts at: 0 287 2219 2409 2649 3528
Size: 287 1932 190 240 879
Fragments arranged by size:
1932 879 287 240 190
BsmBI CGTCTCn'nnnn_
Cuts at: 0 943 2019 3528
Size: 943 1076 1509
Fragments arranged by size:
1509 1076 943
Bsp1286I G_dGCh'C
Cuts at: 0 2606 3528
Size: 2606 922
BspHI T'CATG_A
Cuts at: 0 750 3452 3528
Size: 750 2702 76
Fragments arranged by size:
2702 750 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1754 2275 2653 3528
Size: 1754 521 378 875
Fragments arranged by size:
1754 875 521 378
BsrDI GCAATG_nn'
Cuts at: 0 2802 2912 3528
Size: 2802 110 616
Fragments arranged by size:
2802 616 110
BsrFI r'CCGG_y
Cuts at: 0 1281 3528
Size: 1281 2247
BsrGI T'GTAC_A
Cuts at: 0 2300 3528
Size: 2300 1228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2265 2817 3528
Size: 2265 552 711
Fragments arranged by size:
2265 711 552
BstYI r'GATC_y
Cuts at: 0 454 2025 3528
Size: 454 1571 1503
Fragments arranged by size:
1571 1503 454
BstZ17I GTA'TAC
Cuts at: 0 710 3528
Size: 710 2818
Bsu36I CC'TnA_GG
Cuts at: 0 782 1692 3528
Size: 782 910 1836
Fragments arranged by size:
1836 910 782
BtgI C'CryG_G
Cuts at: 0 2273 3528
Size: 2273 1255
BtsI GCAGTG_nn'
Cuts at: 0 1768 3528
Size: 1768 1760
DraI TTT'AAA
Cuts at: 0 575 1446 2451 3528
Size: 575 871 1005 1077
Fragments arranged by size:
1077 1005 871 575
DrdI GACnn_nn'nnGTC
Cuts at: 0 2908 3528
Size: 2908 620
EaeI y'GGCC_r
Cuts at: 0 2077 3234 3528
Size: 2077 1157 294
Fragments arranged by size:
2077 1157 294
EarI CTCTTCn'nnn_
Cuts at: 0 356 362 500 1601 3528
Size: 356 6 138 1101 1927
Fragments arranged by size:
1927 1101 356 138 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3528
Size: 19 3509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 785 1523 2965 3019 3528
Size: 545 240 738 1442 54 509
Fragments arranged by size:
1442 738 545 509 240 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1828 2660 3394 3528
Size: 1828 832 734 134
Fragments arranged by size:
1828 832 734 134
EcoO109I rG'GnC_Cy
Cuts at: 0 2665 3010 3370 3528
Size: 2665 345 360 158
Fragments arranged by size:
2665 360 345 158
EcoRI G'AATT_C
Cuts at: 0 1987 3528
Size: 1987 1541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1193 1248 3528
Size: 1193 55 2280
Fragments arranged by size:
2280 1193 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2114 2134 2146 2166 2720 2752 2997 3029
Size: 2114 20 12 20 554 32 245 32
Cuts at: 3029 3371 3403 3528
Size: 342 32 125
Fragments arranged by size:
2114 554 342 245 125 32 32 32
20 20 12
HindIII A'AGCT_T
Cuts at: 0 2456 3528
Size: 2456 1072
KpnI G_GTAC'C
Cuts at: 0 228 3528
Size: 228 3300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 756 1205 3528
Size: 84 672 449 2323
Fragments arranged by size:
2323 672 449 84
MscI TGG'CCA
Cuts at: 0 3236 3528
Size: 3236 292
MslI CAynn'nnrTG
Cuts at: 0 1159 1432 1632 2595 2597 2815 3528
Size: 1159 273 200 963 2 218 713
Fragments arranged by size:
1159 963 713 273 218 200 2
MspA1I CmG'CkG
Cuts at: 0 1866 2107 3528
Size: 1866 241 1421
Fragments arranged by size:
1866 1421 241
NcoI C'CATG_G
Cuts at: 0 2273 3528
Size: 2273 1255
NsiI A_TGCA'T
Cuts at: 0 102 3528
Size: 102 3426
NspI r_CATG'y
Cuts at: 0 299 1714 3433 3528
Size: 299 1415 1719 95
Fragments arranged by size:
1719 1415 299 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 1309 3232 3528
Size: 1309 1923 296
Fragments arranged by size:
1923 1309 296
PpuMI rG'GwC_Cy
Cuts at: 0 2665 3528
Size: 2665 863
PshAI GACnn'nnGTC
Cuts at: 0 602 3155 3528
Size: 602 2553 373
Fragments arranged by size:
2553 602 373
PsiI TTA'TAA
Cuts at: 0 2060 2416 3528
Size: 2060 356 1112
Fragments arranged by size:
2060 1112 356
PstI C_TGCA'G
Cuts at: 0 2792 3528
Size: 2792 736
PvuII CAG'CTG
Cuts at: 0 2107 3528
Size: 2107 1421
SexAI A'CCwGG_T
Cuts at: 0 3192 3528
Size: 3192 336
SfcI C'TryA_G
Cuts at: 0 218 2102 2246 2788 3528
Size: 218 1884 144 542 740
Fragments arranged by size:
1884 740 542 218 144
SmlI C'TyrA_G
Cuts at: 0 814 1719 2737 3091 3528
Size: 814 905 1018 354 437
Fragments arranged by size:
1018 905 814 437 354
SnaBI TAC'GTA
Cuts at: 0 2866 3528
Size: 2866 662
SphI G_CATG'C
Cuts at: 0 3433 3528
Size: 3433 95
SspI AAT'ATT
Cuts at: 0 858 3528
Size: 858 2670
StyI C'CwwG_G
Cuts at: 0 489 1750 1892 2273 2836 3528
Size: 489 1261 142 381 563 692
Fragments arranged by size:
1261 692 563 489 381 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2307 3528
Size: 2307 1221
TatI w'GTAC_w
Cuts at: 0 274 935 2300 3401 3528
Size: 274 661 1365 1101 127
Fragments arranged by size:
1365 1101 661 274 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1747 1779 3528
Size: 1747 32 1749
Fragments arranged by size:
1749 1747 32
Tth111I GACn'n_nGTC
Cuts at: 0 1264 3528
Size: 1264 2264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1797 3229 3528
Size: 1797 1432 299
Fragments arranged by size:
1797 1432 299
XmnI GAAnn'nnTTC
Cuts at: 0 1317 3528
Size: 1317 2211
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI ApoI AseI BanI BbsI BciVI BfrBI BmrI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DraI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HindIII KpnI MmeI
MscI MslI MspA1I NcoI NsiI NspI PflMI PpuMI
PshAI PsiI PstI PvuII SexAI SfcI SmlI SnaBI
SphI SspI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsiEI BsiWI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstEII BtgZI ClaI CspCI DraIII
EagI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SpeI SrfI StuI SwaI XbaI
XhoI XmaI ZraI