Restriction map for
pF1KSDA1348
(Linear) (Six-base) MAPSORT of: KIAA1348.seq Check: 9925 from: 1 to: 1587
KIAA1348.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 69 1587
Size: 69 1518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1587
Size: 1078 509
AflIII A'CryG_T
Cuts at: 0 916 1587
Size: 916 671
AleI CACnn'nnGTG
Cuts at: 0 607 626 1540 1587
Size: 607 19 914 47
Fragments arranged by size:
914 607 47 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 822 1587
Size: 822 765
AlwNI CAG_nnn'CTG
Cuts at: 0 507 645 711 1359 1587
Size: 507 138 66 648 228
Fragments arranged by size:
648 507 228 138 66
ApoI r'AATT_y
Cuts at: 0 30 309 827 1587
Size: 30 279 518 760
Fragments arranged by size:
760 518 279 30
AseI AT'TA_AT
Cuts at: 0 691 1587
Size: 691 896
AvaI C'yCGr_G
Cuts at: 0 128 1587
Size: 128 1459
BanI G'GyrC_C
Cuts at: 0 132 1286 1587
Size: 132 1154 301
Fragments arranged by size:
1154 301 132
BanII G_rGCy'C
Cuts at: 0 1349 1388 1587
Size: 1349 39 199
Fragments arranged by size:
1349 199 39
BbvCI CC'TCA_GC
Cuts at: 0 1198 1295 1587
Size: 1198 97 292
Fragments arranged by size:
1198 292 97
BclI T'GATC_A
Cuts at: 0 1421 1587
Size: 1421 166
BfrBI ATG'CAT
Cuts at: 0 439 1587
Size: 439 1148
BlpI GC'TnA_GC
Cuts at: 0 1172 1252 1587
Size: 1172 80 335
Fragments arranged by size:
1172 335 80
Bme1580I G_kGCm'C
Cuts at: 0 447 1289 1587
Size: 447 842 298
Fragments arranged by size:
842 447 298
BmgBI CAC'GTC
Cuts at: 0 1266 1587
Size: 1266 321
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 1024 1587
Size: 349 675 563
Fragments arranged by size:
675 563 349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 529 763 1111 1587
Size: 66 463 234 348 476
Fragments arranged by size:
476 463 348 234 66
Bpu10I CC'TnA_GC
Cuts at: 0 236 1198 1295 1587
Size: 236 962 97 292
Fragments arranged by size:
962 292 236 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1587
Size: 553 1034
BsaI GGTCTCn'nnnn_
Cuts at: 0 1323 1587
Size: 1323 264
BsaAI yAC'GTr
Cuts at: 0 840 919 1587
Size: 840 79 668
Fragments arranged by size:
840 668 79
BsaHI Gr'CG_yC
Cuts at: 0 1019 1587
Size: 1019 568
BsiHKAI G_wGCw'C
Cuts at: 0 515 971 1587
Size: 515 456 616
Fragments arranged by size:
616 515 456
BsmI GAATG_Cn'
Cuts at: 0 1085 1587
Size: 1085 502
Bsp1286I G_dGCh'C
Cuts at: 0 447 515 971 1289 1349 1388 1587
Size: 447 68 456 318 60 39 199
Fragments arranged by size:
456 447 318 199 68 60 39
BsrBI CCG'CTC
Cuts at: 0 1470 1587
Size: 1470 117
BsrDI GCAATG_nn'
Cuts at: 0 47 55 1263 1489 1587
Size: 47 8 1208 226 98
Fragments arranged by size:
1208 226 98 47 8
BsrFI r'CCGG_y
Cuts at: 0 365 1587
Size: 365 1222
BsrGI T'GTAC_A
Cuts at: 0 1514 1587
Size: 1514 73
BssSI C'ACGA_G
Cuts at: 0 1390 1587
Size: 1390 197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 645 1359 1587
Size: 645 714 228
Fragments arranged by size:
714 645 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 823 1587
Size: 823 764
BstYI r'GATC_y
Cuts at: 0 23 1587
Size: 23 1564
Bsu36I CC'TnA_GG
Cuts at: 0 1178 1587
Size: 1178 409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1495 1587
Size: 1495 92
BtsI GCAGTG_nn'
Cuts at: 0 458 488 1587
Size: 458 30 1099
Fragments arranged by size:
1099 458 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 431 1587
Size: 431 1156
EarI CTCTTCn'nnn_
Cuts at: 0 472 1587
Size: 472 1115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 529 763 1078 1111 1587
Size: 66 463 234 315 33 476
Fragments arranged by size:
476 463 315 234 66 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1587
Size: 1294 293
EcoO109I rG'GnC_Cy
Cuts at: 0 741 1037 1113 1202 1587
Size: 741 296 76 89 385
Fragments arranged by size:
741 385 296 89 76
EcoRI G'AATT_C
Cuts at: 0 827 1587
Size: 827 760
EcoRV GAT'ATC
Cuts at: 0 1527 1587
Size: 1527 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 589 621 1418 1450 1587
Size: 589 32 797 32 137
Fragments arranged by size:
797 589 137 32 32
MslI CAynn'nnrTG
Cuts at: 0 440 607 626 664 821 1540 1587
Size: 440 167 19 38 157 719 47
Fragments arranged by size:
719 440 167 157 47 38 19
MspA1I CmG'CkG
Cuts at: 0 339 1056 1382 1587
Size: 339 717 326 205
Fragments arranged by size:
717 339 326 205
NsiI A_TGCA'T
Cuts at: 0 441 1587
Size: 441 1146
NspI r_CATG'y
Cuts at: 0 443 897 1251 1432 1587
Size: 443 454 354 181 155
Fragments arranged by size:
454 443 354 181 155
PasI CC'CwG_GG
Cuts at: 0 577 1587
Size: 577 1010
PflMI CCAn_nnn'nTGG
Cuts at: 0 163 507 711 1587
Size: 163 344 204 876
Fragments arranged by size:
876 344 204 163
PmlI CAC'GTG
Cuts at: 0 840 919 1587
Size: 840 79 668
Fragments arranged by size:
840 668 79
PstI C_TGCA'G
Cuts at: 0 1036 1370 1587
Size: 1036 334 217
Fragments arranged by size:
1036 334 217
PvuII CAG'CTG
Cuts at: 0 339 1056 1587
Size: 339 717 531
Fragments arranged by size:
717 531 339
SapI GCTCTTCn'nnn_
Cuts at: 0 472 1587
Size: 472 1115
SbfI CC_TGCA'GG
Cuts at: 0 1036 1587
Size: 1036 551
ScaI AGT'ACT
Cuts at: 0 9 1587
Size: 9 1578
SfcI C'TryA_G
Cuts at: 0 186 1032 1366 1587
Size: 186 846 334 221
Fragments arranged by size:
846 334 221 186
SmaI CCC'GGG
Cuts at: 0 130 1587
Size: 130 1457
SmlI C'TyrA_G
Cuts at: 0 568 1587
Size: 568 1019
StyI C'CwwG_G
Cuts at: 0 870 1587
Size: 870 717
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 590 1587
Size: 153 437 997
Fragments arranged by size:
997 437 153
TatI w'GTAC_w
Cuts at: 0 7 695 1514 1587
Size: 7 688 819 73
Fragments arranged by size:
819 688 73 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 886 1587
Size: 354 532 701
Fragments arranged by size:
701 532 354
XmaI C'CCGG_G
Cuts at: 0 128 1587
Size: 128 1459
XmnI GAAnn'nnTTC
Cuts at: 0 254 1587
Size: 254 1333
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvaI BanI BanII BbvCI BclI BfrBI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgZI BtsI DrdI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I MslI MspA1I
NsiI NspI PasI PflMI PmlI PstI PvuII SapI
SbfI ScaI SfcI SmaI SmlI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BglI BglII BmtI BplI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BssHII BstBI BstEII BstZ17I
BtgI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoICRI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
ZraI