Restriction map for
pF1KA1346
(Linear) (Six-base) MAPSORT of: KIAA1346.seq Check: 5516 from: 1 to: 2901
KIAA1346.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AflIII A'CryG_T
Cuts at: 0 1096 1214 1513 2901
Size: 1096 118 299 1388
Fragments arranged by size:
1388 1096 299 118
AleI CACnn'nnGTG
Cuts at: 0 824 2901
Size: 824 2077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1822 2901
Size: 1822 1079
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 2901
Size: 1261 32 1608
Fragments arranged by size:
1608 1261 32
AlwNI CAG_nnn'CTG
Cuts at: 0 252 422 480 733 1151 1820 2901
Size: 252 170 58 253 418 669 1081
Fragments arranged by size:
1081 669 418 253 252 170 58
ApaI G_GGCC'C
Cuts at: 0 95 2901
Size: 95 2806
ApoI r'AATT_y
Cuts at: 0 814 2429 2901
Size: 814 1615 472
Fragments arranged by size:
1615 814 472
AseI AT'TA_AT
Cuts at: 0 2651 2901
Size: 2651 250
AvaI C'yCGr_G
Cuts at: 0 16 148 204 2901
Size: 16 132 56 2697
Fragments arranged by size:
2697 132 56 16
BamHI G'GATC_C
Cuts at: 0 2278 2901
Size: 2278 623
BanI G'GyrC_C
Cuts at: 0 152 183 288 379 439 534 1435 1537
Size: 152 31 105 91 60 95 901 102
Cuts at: 1537 1591 2901
Size: 54 1310
Fragments arranged by size:
1310 901 152 105 102 95 91 60
54 31
BanII G_rGCy'C
Cuts at: 0 72 95 614 2459 2901
Size: 72 23 519 1845 442
Fragments arranged by size:
1845 519 442 72 23
BbeI G_GCGC'C
Cuts at: 0 156 292 443 2901
Size: 156 136 151 2458
Fragments arranged by size:
2458 156 151 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 647 2634 2901
Size: 647 1987 267
Fragments arranged by size:
1987 647 267
BbvCI CC'TCA_GC
Cuts at: 0 415 2901
Size: 415 2486
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 785 819 2901
Size: 785 34 2082
Fragments arranged by size:
2082 785 34
BglII A'GATC_T
Cuts at: 0 923 2901
Size: 923 1978
Bme1580I G_kGCm'C
Cuts at: 0 17 95 2901
Size: 17 78 2806
Fragments arranged by size:
2806 78 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 632 1019 1759 2724 2901
Size: 632 387 740 965 177
Fragments arranged by size:
965 740 632 387 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 280 289 2258 2901
Size: 214 66 9 1969 643
Fragments arranged by size:
1969 643 214 66 9
Bpu10I CC'TnA_GC
Cuts at: 0 415 2901
Size: 415 2486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1849 2415 2901
Size: 1849 566 486
Fragments arranged by size:
1849 566 486
BsaI GGTCTCn'nnnn_
Cuts at: 0 83 628 1334 2901
Size: 83 545 706 1567
Fragments arranged by size:
1567 706 545 83
BsaAI yAC'GTr
Cuts at: 0 590 1215 2901
Size: 590 625 1686
Fragments arranged by size:
1686 625 590
BsaHI Gr'CG_yC
Cuts at: 0 153 289 337 440 1291 1952 2901
Size: 153 136 48 103 851 661 949
Fragments arranged by size:
949 851 661 153 136 103 48
BsaWI w'CCGG_w
Cuts at: 0 345 2901
Size: 345 2556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2392 2422 2901
Size: 2392 30 479
Fragments arranged by size:
2392 479 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 187 1745 2400 2901
Size: 178 9 1558 655 501
Fragments arranged by size:
1558 655 501 178 9
BseYI C'CCAG_C
Cuts at: 0 39 397 453 886 1682 2739 2901
Size: 39 358 56 433 796 1057 162
Fragments arranged by size:
1057 796 433 358 162 56 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 2732 2901
Size: 217 2515 169
Fragments arranged by size:
2515 217 169
BsiEI CG_ry'CG
Cuts at: 0 619 2901
Size: 619 2282
BsiHKAI G_wGCw'C
Cuts at: 0 1046 2901
Size: 1046 1855
BsiWI C'GTAC_G
Cuts at: 0 1442 2901
Size: 1442 1459
BsmI GAATG_Cn'
Cuts at: 0 890 2644 2899 2901
Size: 890 1754 255 2
Fragments arranged by size:
1754 890 255 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 328 1959 2901
Size: 328 1631 942
Fragments arranged by size:
1631 942 328
Bsp1286I G_dGCh'C
Cuts at: 0 17 72 95 614 1046 2459 2901
Size: 17 55 23 519 432 1413 442
Fragments arranged by size:
1413 519 442 432 55 23 17
BspEI T'CCGG_A
Cuts at: 0 345 2901
Size: 345 2556
BspHI T'CATG_A
Cuts at: 0 2214 2832 2901
Size: 2214 618 69
Fragments arranged by size:
2214 618 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 458 2901
Size: 458 2443
BsrBI CCG'CTC
Cuts at: 0 66 2901
Size: 66 2835
BsrDI GCAATG_nn'
Cuts at: 0 2493 2901
Size: 2493 408
BsrFI r'CCGG_y
Cuts at: 0 531 1454 1534 2901
Size: 531 923 80 1367
Fragments arranged by size:
1367 923 531 80
BsrGI T'GTAC_A
Cuts at: 0 875 2378 2901
Size: 875 1503 523
Fragments arranged by size:
1503 875 523
BssHII G'CGCG_C
Cuts at: 0 198 2901
Size: 198 2703
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1151 2901
Size: 1151 1750
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 826 2901
Size: 792 34 2075
Fragments arranged by size:
2075 792 34
BstYI r'GATC_y
Cuts at: 0 255 923 1816 2161 2278 2901
Size: 255 668 893 345 117 623
Fragments arranged by size:
893 668 623 345 255 117
BtgI C'CryG_G
Cuts at: 0 119 819 1579 2901
Size: 119 700 760 1322
Fragments arranged by size:
1322 760 700 119
BtsI GCAGTG_nn'
Cuts at: 0 363 703 831 1252 1344 1465 1491 2901
Size: 363 340 128 421 92 121 26 1410
Fragments arranged by size:
1410 421 363 340 128 121 92 26
DraI TTT'AAA
Cuts at: 0 2852 2901
Size: 2852 49
DraIII CAC_nnn'GTG
Cuts at: 0 1027 1526 2901
Size: 1027 499 1375
Fragments arranged by size:
1375 1027 499
DrdI GACnn_nn'nnGTC
Cuts at: 0 1765 2901
Size: 1765 1136
EaeI y'GGCC_r
Cuts at: 0 131 616 2901
Size: 131 485 2285
Fragments arranged by size:
2285 485 131
EagI C'GGCC_G
Cuts at: 0 616 2901
Size: 616 2285
EarI CTCTTCn'nnn_
Cuts at: 0 2576 2901
Size: 2576 325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 217 576 1600 2901
Size: 217 359 1024 1301
Fragments arranged by size:
1301 1024 359 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 280 289 2258 2901
Size: 214 66 9 1969 643
Fragments arranged by size:
1969 643 214 66 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 2901
Size: 424 2477
EcoO109I rG'GnC_Cy
Cuts at: 0 676 696 1088 1696 2901
Size: 676 20 392 608 1205
Fragments arranged by size:
1205 676 608 392 20
EcoRI G'AATT_C
Cuts at: 0 814 2429 2901
Size: 814 1615 472
Fragments arranged by size:
1615 814 472
EcoRV GAT'ATC
Cuts at: 0 2212 2220 2901
Size: 2212 8 681
Fragments arranged by size:
2212 681 8
HaeII r_GCGC'y
Cuts at: 0 127 156 292 443 501 2901
Size: 127 29 136 151 58 2400
Fragments arranged by size:
2400 151 136 127 58 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 2047 2079 2737 2769 2829 2861
Size: 385 32 1630 32 658 32 60 32
Cuts at: 2861 2901
Size: 40
Fragments arranged by size:
1630 658 385 60 40 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1901 2793 2901
Size: 1901 892 108
Fragments arranged by size:
1901 892 108
KasI G'GCGC_C
Cuts at: 0 152 288 439 2901
Size: 152 136 151 2462
Fragments arranged by size:
2462 152 151 136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1104 1328 2257 2901
Size: 119 985 224 929 644
Fragments arranged by size:
985 929 644 224 119
MslI CAynn'nnrTG
Cuts at: 0 773 790 824 1235 2254 2901
Size: 773 17 34 411 1019 647
Fragments arranged by size:
1019 773 647 411 34 17
MspA1I CmG'CkG
Cuts at: 0 121 252 480 1598 2265 2405 2739 2901
Size: 121 131 228 1118 667 140 334 162
Fragments arranged by size:
1118 667 334 228 162 140 131 121
NaeI GCC'GGC
Cuts at: 0 533 2901
Size: 533 2368
NarI GG'CG_CC
Cuts at: 0 153 289 440 2901
Size: 153 136 151 2461
Fragments arranged by size:
2461 153 151 136
NgoMIV G'CCGG_C
Cuts at: 0 531 2901
Size: 531 2370
NspI r_CATG'y
Cuts at: 0 1100 1227 1517 2901
Size: 1100 127 290 1384
Fragments arranged by size:
1384 1100 290 127
PasI CC'CwG_GG
Cuts at: 0 517 2901
Size: 517 2384
PciI A'CATG_T
Cuts at: 0 1096 1513 2901
Size: 1096 417 1388
Fragments arranged by size:
1388 1096 417
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 312 807 1287 1777 2901
Size: 252 60 495 480 490 1124
Fragments arranged by size:
1124 495 490 480 252 60
PfoI T'CCnGG_A
Cuts at: 0 2281 2901
Size: 2281 620
PmlI CAC'GTG
Cuts at: 0 590 1215 2901
Size: 590 625 1686
Fragments arranged by size:
1686 625 590
PpuMI rG'GwC_Cy
Cuts at: 0 696 1088 2901
Size: 696 392 1813
Fragments arranged by size:
1813 696 392
PspOMI G'GGCC_C
Cuts at: 0 91 2901
Size: 91 2810
PvuII CAG'CTG
Cuts at: 0 252 1598 2739 2901
Size: 252 1346 1141 162
Fragments arranged by size:
1346 1141 252 162
SacII CC_GC'GG
Cuts at: 0 122 2901
Size: 122 2779
SanDI GG'GwC_CC
Cuts at: 0 1088 2901
Size: 1088 1813
ScaI AGT'ACT
Cuts at: 0 1789 2901
Size: 1789 1112
SfcI C'TryA_G
Cuts at: 0 541 1812 2901
Size: 541 1271 1089
Fragments arranged by size:
1271 1089 541
SfoI GGC'GCC
Cuts at: 0 154 290 441 2901
Size: 154 136 151 2460
Fragments arranged by size:
2460 154 151 136
SmaI CCC'GGG
Cuts at: 0 206 2901
Size: 206 2695
SmlI C'TyrA_G
Cuts at: 0 1828 2394 2901
Size: 1828 566 507
Fragments arranged by size:
1828 566 507
SpeI A'CTAG_T
Cuts at: 0 2194 2901
Size: 2194 707
StyI C'CwwG_G
Cuts at: 0 1324 1699 2024 2901
Size: 1324 375 325 877
Fragments arranged by size:
1324 877 375 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 652 1038 2901
Size: 652 386 1863
Fragments arranged by size:
1863 652 386
TatI w'GTAC_w
Cuts at: 0 875 1739 1787 2378 2901
Size: 875 864 48 591 523
Fragments arranged by size:
875 864 591 523 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 530 562 1776 1808 2901
Size: 530 32 1214 32 1093
Fragments arranged by size:
1214 1093 530 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1471 2901
Size: 1471 1430
XmaI C'CCGG_G
Cuts at: 0 204 2901
Size: 204 2697
XmnI GAAnn'nnTTC
Cuts at: 0 1934 2429 2901
Size: 1934 495 472
Fragments arranged by size:
1934 495 472
Enzymes that do cut:
AflIII AleI AlfI AloI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HindIII
KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PvuII
SacII SanDI ScaI SfcI SfoI SmaI SmlI SpeI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI ApaLI AscI AsiSI AvrII BaeI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BsaBI
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
EcoICRI FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SalI SapI SbfI SexAI
SfiI SgfI SgrAI SnaBI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI ZraI