Restriction map for
pF1KA1333
(Linear) (Six-base) MAPSORT of: KIAA1333.seq Check: 7988 from: 1 to: 2118
KIAA1333.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 2118
Size: 307 1811
AfeI AGC'GCT
Cuts at: 0 769 2118
Size: 769 1349
AflIII A'CryG_T
Cuts at: 0 900 2118
Size: 900 1218
AhdI GACnn_n'nnGTC
Cuts at: 0 1530 2118
Size: 1530 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1929 2118
Size: 1929 189
AlwNI CAG_nnn'CTG
Cuts at: 0 1686 2118
Size: 1686 432
ApoI r'AATT_y
Cuts at: 0 149 445 617 1021 1231 1308 1712 2118
Size: 149 296 172 404 210 77 404 406
Fragments arranged by size:
406 404 404 296 210 172 149 77
AseI AT'TA_AT
Cuts at: 0 1048 2118
Size: 1048 1070
BamHI G'GATC_C
Cuts at: 0 985 2118
Size: 985 1133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1895 2118
Size: 1895 223
BfrBI ATG'CAT
Cuts at: 0 1132 2118
Size: 1132 986
BglII A'GATC_T
Cuts at: 0 999 2118
Size: 999 1119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1913 2118
Size: 1913 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 1298 1582 2118
Size: 1279 19 284 536
Fragments arranged by size:
1279 536 284 19
BsaBI GATnn'nnATC
Cuts at: 0 1492 2118
Size: 1492 626
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 801 2118
Size: 801 1317
BseYI C'CCAG_C
Cuts at: 0 1445 2118
Size: 1445 673
BsmI GAATG_Cn'
Cuts at: 0 1132 1552 2118
Size: 1132 420 566
Fragments arranged by size:
1132 566 420
BsrGI T'GTAC_A
Cuts at: 0 117 565 2118
Size: 117 448 1553
Fragments arranged by size:
1553 448 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 437 541 2118
Size: 437 104 1577
Fragments arranged by size:
1577 437 104
BstBI TT'CG_AA
Cuts at: 0 705 2118
Size: 705 1413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 349 441 2118
Size: 349 92 1677
Fragments arranged by size:
1677 349 92
BstYI r'GATC_y
Cuts at: 0 985 999 2118
Size: 985 14 1119
Fragments arranged by size:
1119 985 14
DraI TTT'AAA
Cuts at: 0 1941 2118
Size: 1941 177
EarI CTCTTCn'nnn_
Cuts at: 0 944 2118
Size: 944 1174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1913 2118
Size: 307 1606 205
Fragments arranged by size:
1606 307 205
EcoO109I rG'GnC_Cy
Cuts at: 0 1283 2118
Size: 1283 835
EcoRI G'AATT_C
Cuts at: 0 617 2118
Size: 617 1501
EcoRV GAT'ATC
Cuts at: 0 204 2118
Size: 204 1914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1348 1645 1912 2118
Size: 1348 297 267 206
Fragments arranged by size:
1348 297 267 206
HaeII r_GCGC'y
Cuts at: 0 771 2118
Size: 771 1347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 984 1016 1079 1111 1252 1284 1516 1548
Size: 984 32 63 32 141 32 232 32
Cuts at: 1548 1855 1887 2118
Size: 307 32 231
Fragments arranged by size:
984 307 232 231 141 63 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1823 2118
Size: 1823 295
MfeI C'AATT_G
Cuts at: 0 269 1166 1955 2118
Size: 269 897 789 163
Fragments arranged by size:
897 789 269 163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1965 2118
Size: 926 1039 153
Fragments arranged by size:
1039 926 153
MslI CAynn'nnrTG
Cuts at: 0 288 2118
Size: 288 1830
MspA1I CmG'CkG
Cuts at: 0 1933 2118
Size: 1933 185
NdeI CA'TA_TG
Cuts at: 0 1161 1179 2118
Size: 1161 18 939
Fragments arranged by size:
1161 939 18
NsiI A_TGCA'T
Cuts at: 0 1134 2118
Size: 1134 984
NspI r_CATG'y
Cuts at: 0 904 1972 2118
Size: 904 1068 146
Fragments arranged by size:
1068 904 146
PciI A'CATG_T
Cuts at: 0 900 2118
Size: 900 1218
PflMI CCAn_nnn'nTGG
Cuts at: 0 1112 2118
Size: 1112 1006
PfoI T'CCnGG_A
Cuts at: 0 1215 2118
Size: 1215 903
PpuMI rG'GwC_Cy
Cuts at: 0 1283 2118
Size: 1283 835
PsiI TTA'TAA
Cuts at: 0 470 1492 1588 2118
Size: 470 1022 96 530
Fragments arranged by size:
1022 530 470 96
PstI C_TGCA'G
Cuts at: 0 683 2118
Size: 683 1435
PvuII CAG'CTG
Cuts at: 0 1933 2118
Size: 1933 185
SexAI A'CCwGG_T
Cuts at: 0 15 1389 2118
Size: 15 1374 729
Fragments arranged by size:
1374 729 15
SfcI C'TryA_G
Cuts at: 0 679 1517 2118
Size: 679 838 601
Fragments arranged by size:
838 679 601
SmlI C'TyrA_G
Cuts at: 0 1258 1313 1561 2118
Size: 1258 55 248 557
Fragments arranged by size:
1258 557 248 55
StyI C'CwwG_G
Cuts at: 0 1695 2118
Size: 1695 423
TatI w'GTAC_w
Cuts at: 0 117 565 1800 2118
Size: 117 448 1235 318
Fragments arranged by size:
1235 448 318 117
XmnI GAAnn'nnTTC
Cuts at: 0 1892 2118
Size: 1892 226
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI AlwNI ApoI AseI
BamHI BbsI BfrBI BglII BpmI BpuEI BsaBI BseRI
BseYI BsmI BsrGI BstAPI BstBI BstXI BstYI DraI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HindIII MfeI MmeI MslI MspA1I NdeI NsiI NspI
PciI PflMI PfoI PpuMI PsiI PstI PvuII SexAI
SfcI SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HincII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI