Restriction map for
pF1KA1305
(Linear) (Six-base) MAPSORT of: KIAA1305.seq Check: 1742 from: 1 to: 5694
KIAA1305.orf
With 182 enzymes: SgfI *
December 13, 2010 18:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1917 1977 1995 2049 2282 5694
Size: 1917 60 18 54 233 3412
Fragments arranged by size:
3412 1917 233 60 54 18
AatII G_ACGT'C
Cuts at: 0 5363 5694
Size: 5363 331
AccI GT'mk_AC
Cuts at: 0 1296 4828 5694
Size: 1296 3532 866
Fragments arranged by size:
3532 1296 866
Acc65I G'GTAC_C
Cuts at: 0 2309 5694
Size: 2309 3385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 2161 2542 2851 2893 3442 4128 4972
Size: 226 1935 381 309 42 549 686 844
Cuts at: 4972 4975 5226 5694
Size: 3 251 468
Fragments arranged by size:
1935 844 686 549 468 381 309 251
226 42 3
AfeI AGC'GCT
Cuts at: 0 4090 5694
Size: 4090 1604
AflIII A'CryG_T
Cuts at: 0 3932 5694
Size: 3932 1762
AleI CACnn'nnGTG
Cuts at: 0 3679 4972 5694
Size: 3679 1293 722
Fragments arranged by size:
3679 1293 722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3251 5018 5694
Size: 3251 1767 676
Fragments arranged by size:
3251 1767 676
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5110 5142 5694
Size: 5110 32 552
Fragments arranged by size:
5110 552 32
AlwNI CAG_nnn'CTG
Cuts at: 0 84 561 1603 1621 1747 3831 3891 4500
Size: 84 477 1042 18 126 2084 60 609
Cuts at: 4500 4598 4754 4986 5694
Size: 98 156 232 708
Fragments arranged by size:
2084 1042 708 609 477 232 156 126
98 84 60 18
ApaI G_GGCC'C
Cuts at: 0 173 282 433 495 763 1195 2253 2945
Size: 173 109 151 62 268 432 1058 692
Cuts at: 2945 3153 3349 3604 3645 3807 4665 4870 5048
Size: 208 196 255 41 162 858 205 178
Cuts at: 5048 5175 5694
Size: 127 519
Fragments arranged by size:
1058 858 692 519 432 268 255 208
205 196 178 173 162 151 127 109
62 41
ApaLI G'TGCA_C
Cuts at: 0 1735 5694
Size: 1735 3959
ApoI r'AATT_y
Cuts at: 0 768 5161 5694
Size: 768 4393 533
Fragments arranged by size:
4393 768 533
AvaI C'yCGr_G
Cuts at: 0 65 163 173 452 479 1053 4181 5694
Size: 65 98 10 279 27 574 3128 1513
Fragments arranged by size:
3128 1513 574 279 98 65 27 10
BamHI G'GATC_C
Cuts at: 0 503 5694
Size: 503 5191
BanI G'GyrC_C
Cuts at: 0 330 2055 2185 2309 2781 3119 4342 5662
Size: 330 1725 130 124 472 338 1223 1320
Cuts at: 5662 5694
Size: 32
Fragments arranged by size:
1725 1320 1223 472 338 330 130 124
32
BanII G_rGCy'C
Cuts at: 0 173 282 433 495 763 1195 1396 1611
Size: 173 109 151 62 268 432 201 215
Cuts at: 1611 2031 2231 2253 2945 3153 3349 3432 3604
Size: 420 200 22 692 208 196 83 172
Cuts at: 3604 3645 3807 4163 4182 4209 4303 4665 4870
Size: 41 162 356 19 27 94 362 205
Cuts at: 4870 5048 5097 5146 5175 5312 5351 5399 5694
Size: 178 49 49 29 137 39 48 295
Fragments arranged by size:
692 432 420 362 356 295 268 215
208 205 201 200 196 178 173 172
162 151 137 109 94 83 62 49
49 48 41 39 29 27 22 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 2890 5355 5694
Size: 605 2285 2465 339
Fragments arranged by size:
2465 2285 605 339
BbvCI CC'TCA_GC
Cuts at: 0 470 2197 3041 3124 5694
Size: 470 1727 844 83 2570
Fragments arranged by size:
2570 1727 844 470 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2248 2282 4456 4490 5694
Size: 2248 34 2174 34 1204
Fragments arranged by size:
2248 2174 1204 34 34
BclI T'GATC_A
Cuts at: 0 809 5694
Size: 809 4885
BglI GCCn_nnn'nGGC
Cuts at: 0 344 490 626 1077 1554 2807 3244 3381
Size: 344 146 136 451 477 1253 437 137
Cuts at: 3381 3415 3514 4063 5101 5245 5694
Size: 34 99 549 1038 144 449
Fragments arranged by size:
1253 1038 549 477 451 449 437 344
146 144 137 136 99 34
BlpI GC'TnA_GC
Cuts at: 0 568 616 2901 5566 5694
Size: 568 48 2285 2665 128
Fragments arranged by size:
2665 2285 568 128 48
Bme1580I G_kGCm'C
Cuts at: 0 173 282 433 495 763 1033 1195 1640
Size: 173 109 151 62 268 270 162 445
Cuts at: 1640 1658 1739 2060 2253 2945 3028 3094 3153
Size: 18 81 321 193 692 83 66 59
Cuts at: 3153 3349 3604 3645 3807 4347 4665 4870 5048
Size: 196 255 41 162 540 318 205 178
Cuts at: 5048 5175 5694
Size: 127 519
Fragments arranged by size:
692 540 519 445 321 318 270 268
255 205 196 193 178 173 162 162
151 127 109 83 81 66 62 59
41 18
BmgBI CAC'GTC
Cuts at: 0 5064 5694
Size: 5064 630
BmrI ACTGGGnnnn_n'
Cuts at: 0 809 2169 3600 4105 5043 5166 5694
Size: 809 1360 1431 505 938 123 528
Fragments arranged by size:
1431 1360 938 809 528 505 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 421 733 992 1125 1261 1493 1523
Size: 330 91 312 259 133 136 232 30
Cuts at: 1523 1625 1659 2007 2968 3505 3808 4105 4194
Size: 102 34 348 961 537 303 297 89
Cuts at: 4194 5050 5107 5413 5424 5694
Size: 856 57 306 11 270
Fragments arranged by size:
961 856 537 348 330 312 306 303
297 270 259 232 136 133 102 91
89 57 34 30 11
Bpu10I CC'TnA_GC
Cuts at: 0 470 2197 3041 3124 5145 5694
Size: 470 1727 844 83 2021 549
Fragments arranged by size:
2021 1727 844 549 470 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1559 1667 1829 1919 2560 2626 3349
Size: 878 681 108 162 90 641 66 723
Cuts at: 3349 3631 5161 5335 5694
Size: 282 1530 174 359
Fragments arranged by size:
1530 878 723 681 641 359 282 174
162 108 90 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 1176 3988 4675 5694
Size: 1176 2812 687 1019
Fragments arranged by size:
2812 1176 1019 687
BsaAI yAC'GTr
Cuts at: 0 3504 5694
Size: 3504 2190
BsaBI GATnn'nnATC
Cuts at: 0 2701 5694
Size: 2701 2993
BsaHI Gr'CG_yC
Cuts at: 0 3070 5360 5694
Size: 3070 2290 334
Fragments arranged by size:
3070 2290 334
BsaWI w'CCGG_w
Cuts at: 0 651 2531 4619 5694
Size: 651 1880 2088 1075
Fragments arranged by size:
2088 1880 1075 651
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 628 658 2419 2449 2877 2907 3079 3109
Size: 628 30 1761 30 428 30 172 30
Cuts at: 3109 3563 3593 4273 4303 5084 5114 5299 5329
Size: 454 30 680 30 781 30 185 30
Cuts at: 5329 5458 5488 5694
Size: 129 30 206
Fragments arranged by size:
1761 781 680 628 454 428 206 185
172 129 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 985 1256 1331 2677 3004 3019 3058
Size: 418 567 271 75 1346 327 15 39
Cuts at: 3058 3121 3352 3418 3568 3604 3811 4185 4500
Size: 63 231 66 150 36 207 374 315
Cuts at: 4500 4900 5241 5694
Size: 400 341 453
Fragments arranged by size:
1346 567 453 418 400 374 341 327
315 271 231 207 150 75 66 63
39 36 15
BseYI C'CCAG_C
Cuts at: 0 425 546 674 693 1287 1311 2600 2896
Size: 425 121 128 19 594 24 1289 296
Cuts at: 2896 3248 3262 3467 3834 4489 4704 5613 5629
Size: 352 14 205 367 655 215 909 16
Cuts at: 5629 5694
Size: 65
Fragments arranged by size:
1289 909 655 594 425 367 352 296
215 205 128 121 65 24 19 16
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 91 112 237 244 1298 2482 3337
Size: 67 24 21 125 7 1054 1184 855
Cuts at: 3337 5215 5694
Size: 1878 479
Fragments arranged by size:
1878 1184 1054 855 479 125 67 24
21 7
BsiEI CG_ry'CG
Cuts at: 0 4832 5694
Size: 4832 862
BsiHKAI G_wGCw'C
Cuts at: 0 316 826 894 1396 1611 1739 2031 3536
Size: 316 510 68 502 215 128 292 1505
Cuts at: 3536 4163 5146 5399 5694
Size: 627 983 253 295
Fragments arranged by size:
1505 983 627 510 502 316 295 292
253 215 128 68
BsmI GAATG_Cn'
Cuts at: 0 1122 4942 5694
Size: 1122 3820 752
Fragments arranged by size:
3820 1122 752
Bsp1286I G_dGCh'C
Cuts at: 0 173 282 316 433 495 763 826 894
Size: 173 109 34 117 62 268 63 68
Cuts at: 894 1033 1195 1396 1611 1640 1658 1739 2031
Size: 139 162 201 215 29 18 81 292
Cuts at: 2031 2060 2231 2253 2945 3028 3094 3153 3349
Size: 29 171 22 692 83 66 59 196
Cuts at: 3349 3432 3536 3604 3645 3807 4163 4182 4209
Size: 83 104 68 41 162 356 19 27
Cuts at: 4209 4303 4347 4665 4870 5048 5097 5146 5175
Size: 94 44 318 205 178 49 49 29
Cuts at: 5175 5312 5351 5399 5694
Size: 137 39 48 295
Fragments arranged by size:
692 356 318 295 292 268 215 205
201 196 178 173 171 162 162 139
137 117 109 104 94 83 83 81
68 68 66 63 62 59 49 49
48 44 41 39 34 29 29 29
27 22 19 18
BspEI T'CCGG_A
Cuts at: 0 651 4619 5694
Size: 651 3968 1075
Fragments arranged by size:
3968 1075 651
BspHI T'CATG_A
Cuts at: 0 383 5694
Size: 383 5311
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 960 1903 1917 1977 1995 2049 2282 3219
Size: 960 943 14 60 18 54 233 937
Cuts at: 3219 4856 5694
Size: 1637 838
Fragments arranged by size:
1637 960 943 937 838 233 60 54
18 14
BsrBI CCG'CTC
Cuts at: 0 1537 5694
Size: 1537 4157
BsrDI GCAATG_nn'
Cuts at: 0 4472 5694
Size: 4472 1222
BsrFI r'CCGG_y
Cuts at: 0 5561 5694
Size: 5561 133
BssSI C'ACGA_G
Cuts at: 0 2314 5694
Size: 2314 3380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 2421 4754 5694
Size: 84 2337 2333 940
Fragments arranged by size:
2337 2333 940 84
BstEII G'GTnAC_C
Cuts at: 0 474 511 3489 4872 5485 5694
Size: 474 37 2978 1383 613 209
Fragments arranged by size:
2978 1383 613 474 209 37
BstXI CCAn_nnnn'nTGG
Cuts at: 0 230 2182 4354 4434 4935 5538 5694
Size: 230 1952 2172 80 501 603 156
Fragments arranged by size:
2172 1952 603 501 230 156 80
BstYI r'GATC_y
Cuts at: 0 458 503 1422 3110 4139 4626 5694
Size: 458 45 919 1688 1029 487 1068
Fragments arranged by size:
1688 1068 1029 919 487 458 45
Bsu36I CC'TnA_GG
Cuts at: 0 3725 4822 5694
Size: 3725 1097 872
Fragments arranged by size:
3725 1097 872
BtgI C'CryG_G
Cuts at: 0 507 698 707 1568 1640 2060 2408 2453
Size: 507 191 9 861 72 420 348 45
Cuts at: 2453 3860 4070 4412 4813 5694
Size: 1407 210 342 401 881
Fragments arranged by size:
1407 881 861 507 420 401 348 342
210 191 72 45 9
BtsI GCAGTG_nn'
Cuts at: 0 1658 1739 2406 2465 3391 3713 4128 4366
Size: 1658 81 667 59 926 322 415 238
Cuts at: 4366 5532 5694
Size: 1166 162
Fragments arranged by size:
1658 1166 926 667 415 322 238 162
81 59
ClaI AT'CG_AT
Cuts at: 0 2390 5694
Size: 2390 3304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4539 4574 5694
Size: 4539 35 1120
Fragments arranged by size:
4539 1120 35
DraIII CAC_nnn'GTG
Cuts at: 0 4931 5694
Size: 4931 763
DrdI GACnn_nn'nnGTC
Cuts at: 0 1593 5694
Size: 1593 4101
EaeI y'GGCC_r
Cuts at: 0 1078 1799 2405 3515 4095 4331 4463 5694
Size: 1078 721 606 1110 580 236 132 1231
Fragments arranged by size:
1231 1110 1078 721 606 580 236 132
EarI CTCTTCn'nnn_
Cuts at: 0 295 1003 1520 2779 2975 3066 4673 5694
Size: 295 708 517 1259 196 91 1607 1021
Fragments arranged by size:
1607 1259 1021 708 517 295 196 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3469 5694
Size: 3469 2225
EcoICRI GAG'CTC
Cuts at: 0 1394 1609 2029 4161 5144 5397 5694
Size: 1394 215 420 2132 983 253 297
Fragments arranged by size:
2132 1394 983 420 297 253 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 330 421 733 992 1125 1261 1493
Size: 226 104 91 312 259 133 136 232
Cuts at: 1493 1523 1625 1659 2007 2161 2542 2851 2893
Size: 30 102 34 348 154 381 309 42
Cuts at: 2893 2968 3442 3505 3808 4105 4128 4194 4972
Size: 75 474 63 303 297 23 66 778
Cuts at: 4972 4975 5050 5107 5226 5413 5424 5694
Size: 3 75 57 119 187 11 270
Fragments arranged by size:
778 474 381 348 312 309 303 297
270 259 232 226 187 154 136 133
119 104 102 91 75 75 66 63
57 42 34 30 23 11 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1104 1150 3103 3526 3823 4549 5694
Size: 1104 46 1953 423 297 726 1145
Fragments arranged by size:
1953 1145 1104 726 423 297 46
EcoO109I rG'GnC_Cy
Cuts at: 0 169 170 210 278 429 430 491 492
Size: 169 1 40 68 151 1 61 1
Cuts at: 492 528 743 759 803 1192 1531 1915 2170
Size: 36 215 16 44 389 339 384 255
Cuts at: 2170 2249 2250 2319 2942 3149 3150 3346 3600
Size: 79 1 69 623 207 1 196 254
Cuts at: 3600 3601 3642 3803 3804 3828 4661 5044 5045
Size: 1 41 161 1 24 833 383 1
Cuts at: 5045 5118 5150 5171 5172 5246 5277 5493 5542
Size: 73 32 21 1 74 31 216 49
Cuts at: 5542 5694
Size: 152
Fragments arranged by size:
833 623 389 384 383 339 255 254
216 215 207 196 169 161 152 151
79 74 73 69 68 61 49 44
41 40 36 32 31 24 21 16
1 1 1 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 0 5161 5694
Size: 5161 533
HaeII r_GCGC'y
Cuts at: 0 4092 5694
Size: 4092 1602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 2372 2404 2877 2909 2920 2952
Size: 628 32 1712 32 473 32 11 32
Cuts at: 2952 3043 3075 3079 3111 3761 3793 5694
Size: 91 32 4 32 650 32 1901
Fragments arranged by size:
1901 1712 650 628 473 91 32 32
32 32 32 32 32 11 4
HincII GTy'rAC
Cuts at: 0 4829 5694
Size: 4829 865
HindIII A'AGCT_T
Cuts at: 0 3755 5694
Size: 3755 1939
KpnI G_GTAC'C
Cuts at: 0 2313 5694
Size: 2313 3381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1026 1798 2828 5694
Size: 890 136 772 1030 2866
Fragments arranged by size:
2866 1030 890 772 136
MscI TGG'CCA
Cuts at: 0 1801 2407 4097 4333 5694
Size: 1801 606 1690 236 1361
Fragments arranged by size:
1801 1690 1361 606 236
MslI CAynn'nnrTG
Cuts at: 0 2458 2707 3314 3679 3931 4898 4972 5694
Size: 2458 249 607 365 252 967 74 722
Fragments arranged by size:
2458 967 722 607 365 252 249 74
MspA1I CmG'CkG
Cuts at: 0 75 81 540 550 849 1291 2520 3471
Size: 75 6 459 10 299 442 1229 951
Cuts at: 3471 3662 3862 4446 5376 5670 5694
Size: 191 200 584 930 294 24
Fragments arranged by size:
1229 951 930 584 459 442 299 294
200 191 75 24 10 6
NcoI C'CATG_G
Cuts at: 0 698 1640 2408 2453 4412 4813 5694
Size: 698 942 768 45 1959 401 881
Fragments arranged by size:
1959 942 881 768 698 401 45
NspI r_CATG'y
Cuts at: 0 523 3936 4735 4903 4999 5694
Size: 523 3413 799 168 96 695
Fragments arranged by size:
3413 799 695 523 168 96
PasI CC'CwG_GG
Cuts at: 0 1020 1387 2796 3203 4347 4592 5040 5088
Size: 1020 367 1409 407 1144 245 448 48
Cuts at: 5088 5694
Size: 606
Fragments arranged by size:
1409 1144 1020 606 448 407 367 245
48
PciI A'CATG_T
Cuts at: 0 3932 5694
Size: 3932 1762
PflMI CCAn_nnn'nTGG
Cuts at: 0 1066 3758 4413 4598 5694
Size: 1066 2692 655 185 1096
Fragments arranged by size:
2692 1096 1066 655 185
PfoI T'CCnGG_A
Cuts at: 0 293 554 720 3686 3706 5694
Size: 293 261 166 2966 20 1988
Fragments arranged by size:
2966 1988 293 261 166 20
PmlI CAC'GTG
Cuts at: 0 3504 5694
Size: 3504 2190
PpuMI rG'GwC_Cy
Cuts at: 0 528 743 803 1531 1915 2170 5118 5277
Size: 528 215 60 728 384 255 2948 159
Cuts at: 5277 5493 5542 5694
Size: 216 49 152
Fragments arranged by size:
2948 728 528 384 255 216 215 159
152 60 49
PspOMI G'GGCC_C
Cuts at: 0 169 278 429 491 759 1191 2249 2941
Size: 169 109 151 62 268 432 1058 692
Cuts at: 2941 3149 3345 3600 3641 3803 4661 4866 5044
Size: 208 196 255 41 162 858 205 178
Cuts at: 5044 5171 5694
Size: 127 523
Fragments arranged by size:
1058 858 692 523 432 268 255 208
205 196 178 169 162 151 127 109
62 41
PspXI vC'TCGA_Gb
Cuts at: 0 163 5694
Size: 163 5531
PstI C_TGCA'G
Cuts at: 0 539 1334 1650 1698 1752 1914 1950 1974
Size: 539 795 316 48 54 162 36 24
Cuts at: 1974 1992 2046 2426 4487 4750 4853 5694
Size: 18 54 380 2061 263 103 841
Fragments arranged by size:
2061 841 795 539 380 316 263 162
103 54 54 48 36 24 18
PvuII CAG'CTG
Cuts at: 0 540 849 1291 2520 3471 4446 5376 5694
Size: 540 309 442 1229 951 975 930 318
Fragments arranged by size:
1229 975 951 930 540 442 318 309
SacI G_AGCT'C
Cuts at: 0 1396 1611 2031 4163 5146 5399 5694
Size: 1396 215 420 2132 983 253 295
Fragments arranged by size:
2132 1396 983 420 295 253 215
SacII CC_GC'GG
Cuts at: 0 3863 5694
Size: 3863 1831
SalI G'TCGA_C
Cuts at: 0 4827 5694
Size: 4827 867
SanDI GG'GwC_CC
Cuts at: 0 743 803 1531 2170 5542 5694
Size: 743 60 728 639 3372 152
Fragments arranged by size:
3372 743 728 639 152 60
SbfI CC_TGCA'GG
Cuts at: 0 1914 5694
Size: 1914 3780
SexAI A'CCwGG_T
Cuts at: 0 326 4927 5489 5694
Size: 326 4601 562 205
Fragments arranged by size:
4601 562 326 205
SfcI C'TryA_G
Cuts at: 0 157 535 1330 1465 1646 1694 1748 1892
Size: 157 378 795 135 181 48 54 144
Cuts at: 1892 1910 1946 1952 1970 1988 2042 2422 4394
Size: 18 36 6 18 18 54 380 1972
Cuts at: 4394 4483 4746 4849 5371 5580 5694
Size: 89 263 103 522 209 114
Fragments arranged by size:
1972 795 522 380 378 263 209 181
157 144 135 114 103 89 54 54
48 36 18 18 18 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 490 1077 2807 3415 3514 5245 5694
Size: 490 587 1730 608 99 1731 449
Fragments arranged by size:
1731 1730 608 587 490 449 99
SmaI CCC'GGG
Cuts at: 0 454 481 1055 5694
Size: 454 27 574 4639
Fragments arranged by size:
4639 574 454 27
SmlI C'TyrA_G
Cuts at: 0 163 893 1574 1682 1844 1934 2575 2605
Size: 163 730 681 108 162 90 641 30
Cuts at: 2605 3364 3646 5176 5350 5694
Size: 759 282 1530 174 344
Fragments arranged by size:
1530 759 730 681 641 344 282 174
163 162 108 90 30
SphI G_CATG'C
Cuts at: 0 523 5694
Size: 523 5171
StuI AGG'CCT
Cuts at: 0 287 1045 3409 5647 5694
Size: 287 758 2364 2238 47
Fragments arranged by size:
2364 2238 758 287 47
StyI C'CwwG_G
Cuts at: 0 194 273 282 698 1342 1640 2078 2189
Size: 194 79 9 416 644 298 438 111
Cuts at: 2189 2254 2408 2453 2816 4412 4655 4813 5694
Size: 65 154 45 363 1596 243 158 881
Fragments arranged by size:
1596 881 644 438 416 363 298 243
194 158 154 111 79 65 45 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1941 4235 4923 5473 5526 5694
Size: 1012 929 2294 688 550 53 168
Fragments arranged by size:
2294 1012 929 688 550 168 53
TatI w'GTAC_w
Cuts at: 0 4902 5694
Size: 4902 792
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 495 527 716 748 2419 2451 3952 3984
Size: 495 32 189 32 1671 32 1501 32
Cuts at: 3984 5084 5116 5299 5331 5458 5469 5490 5501
Size: 1100 32 183 32 127 11 21 11
Cuts at: 5501 5694
Size: 193
Fragments arranged by size:
1671 1501 1100 495 193 189 183 127
32 32 32 32 32 32 21 11
11
Tth111I GACn'n_nGTC
Cuts at: 0 372 5491 5694
Size: 372 5119 203
Fragments arranged by size:
5119 372 203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 705 1468 2155 2187 2608 2727 4928 5694
Size: 705 763 687 32 421 119 2201 766
Fragments arranged by size:
2201 766 763 705 687 421 119 32
XhoI C'TCGA_G
Cuts at: 0 163 5694
Size: 163 5531
XmaI C'CCGG_G
Cuts at: 0 452 479 1053 5694
Size: 452 27 574 4641
Fragments arranged by size:
4641 574 452 27
XmnI GAAnn'nnTTC
Cuts at: 0 121 2773 5694
Size: 121 2652 2921
Fragments arranged by size:
2921 2652 121
ZraI GAC'GTC
Cuts at: 0 5361 5694
Size: 5361 333
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NcoI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SalI SanDI SbfI SexAI
SfcI SfiI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BfrBI BglII BmtI BplI BsiWI
BsmBI BsrGI BssHII BstBI BstZ17I BtgZI DraI EagI
EcoRV FalI FseI FspI FspAI HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PsrI PvuI
RsrII SapI ScaI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI