Restriction map for
pF1KA1291
(Linear) (Six-base) MAPSORT of: KIAA1291.seq Check: 9300 from: 1 to: 3612
KIAA1291.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 2174 3612
Size: 2174 1438
Acc65I G'GTAC_C
Cuts at: 0 3320 3612
Size: 3320 292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 289 552 772 976 1119 3612
Size: 187 102 263 220 204 143 2493
Fragments arranged by size:
2493 263 220 204 187 143 102
AfeI AGC'GCT
Cuts at: 0 82 3080 3612
Size: 82 2998 532
Fragments arranged by size:
2998 532 82
AflIII A'CryG_T
Cuts at: 0 216 3612
Size: 216 3396
AhdI GACnn_n'nnGTC
Cuts at: 0 711 3612
Size: 711 2901
AleI CACnn'nnGTG
Cuts at: 0 2957 3223 3612
Size: 2957 266 389
Fragments arranged by size:
2957 389 266
AlwNI CAG_nnn'CTG
Cuts at: 0 210 1021 2036 2307 3612
Size: 210 811 1015 271 1305
Fragments arranged by size:
1305 1015 811 271 210
ApaLI G'TGCA_C
Cuts at: 0 2153 3612
Size: 2153 1459
ApoI r'AATT_y
Cuts at: 0 1437 1574 3612
Size: 1437 137 2038
Fragments arranged by size:
2038 1437 137
AseI AT'TA_AT
Cuts at: 0 3085 3612
Size: 3085 527
AvaI C'yCGr_G
Cuts at: 0 1316 1781 2897 3612
Size: 1316 465 1116 715
Fragments arranged by size:
1316 1116 715 465
AvrII C'CTAG_G
Cuts at: 0 2550 3612
Size: 2550 1062
BamHI G'GATC_C
Cuts at: 0 1359 1632 2133 3612
Size: 1359 273 501 1479
Fragments arranged by size:
1479 1359 501 273
BanI G'GyrC_C
Cuts at: 0 2019 3320 3612
Size: 2019 1301 292
Fragments arranged by size:
2019 1301 292
BanII G_rGCy'C
Cuts at: 0 1131 3612
Size: 1131 2481
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 802 851 1476 1710 2475 3612
Size: 628 174 49 625 234 765 1137
Fragments arranged by size:
1137 765 628 625 234 174 49
BbvCI CC'TCA_GC
Cuts at: 0 3015 3612
Size: 3015 597
BciVI GTATCCnnnnn_n'
Cuts at: 0 2745 3612
Size: 2745 867
BclI T'GATC_A
Cuts at: 0 368 3612
Size: 368 3244
BfrBI ATG'CAT
Cuts at: 0 888 3612
Size: 888 2724
BglI GCCn_nnn'nGGC
Cuts at: 0 2383 3612
Size: 2383 1229
BlpI GC'TnA_GC
Cuts at: 0 2622 3612
Size: 2622 990
Bme1580I G_kGCm'C
Cuts at: 0 1941 2157 3612
Size: 1941 216 1455
Fragments arranged by size:
1941 1455 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 717 3612
Size: 717 2895
BmtI G_CTAG'C
Cuts at: 0 2377 3612
Size: 2377 1235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2798 3612
Size: 2798 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1481 2149 2890 3346 3577 3612
Size: 772 709 668 741 456 231 35
Fragments arranged by size:
772 741 709 668 456 231 35
Bpu10I CC'TnA_GC
Cuts at: 0 3015 3612
Size: 3015 597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 174 2030 2843 3612
Size: 85 89 1856 813 769
Fragments arranged by size:
1856 813 769 89 85
BsaAI yAC'GTr
Cuts at: 0 3209 3612
Size: 3209 403
BsaBI GATnn'nnATC
Cuts at: 0 463 3612
Size: 463 3149
BsaWI w'CCGG_w
Cuts at: 0 848 3612
Size: 848 2764
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 247 277 745 775 917 947
Size: 55 30 162 30 468 30 142 30
Cuts at: 947 2122 2152 3612
Size: 1175 30 1460
Fragments arranged by size:
1460 1175 468 162 142 55 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 337 938 1302 1815 1888 2023 2887
Size: 127 210 601 364 513 73 135 864
Cuts at: 2887 3154 3612
Size: 267 458
Fragments arranged by size:
864 601 513 458 364 267 210 135
127 73
BseYI C'CCAG_C
Cuts at: 0 77 984 1008 1384 1847 2236 2609 2967
Size: 77 907 24 376 463 389 373 358
Cuts at: 2967 3612
Size: 645
Fragments arranged by size:
907 645 463 389 376 373 358 77
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1522 1655 2277 3612
Size: 1034 488 133 622 1335
Fragments arranged by size:
1335 1034 622 488 133
BsiHKAI G_wGCw'C
Cuts at: 0 1323 2157 2702 2720 2964 3612
Size: 1323 834 545 18 244 648
Fragments arranged by size:
1323 834 648 545 244 18
BsmI GAATG_Cn'
Cuts at: 0 2180 2510 3612
Size: 2180 330 1102
Fragments arranged by size:
2180 1102 330
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2972 3553 3612
Size: 2972 581 59
Fragments arranged by size:
2972 581 59
Bsp1286I G_dGCh'C
Cuts at: 0 1131 1323 1941 2157 2702 2720 2964 3612
Size: 1131 192 618 216 545 18 244 648
Fragments arranged by size:
1131 648 618 545 244 216 192 18
BspHI T'CATG_A
Cuts at: 0 56 3612
Size: 56 3556
BsrDI GCAATG_nn'
Cuts at: 0 815 1003 3458 3612
Size: 815 188 2455 154
Fragments arranged by size:
2455 815 188 154
BsrFI r'CCGG_y
Cuts at: 0 86 3612
Size: 86 3526
BsrGI T'GTAC_A
Cuts at: 0 3073 3612
Size: 3073 539
BstAPI GCAn_nnn'nTGC
Cuts at: 0 913 2061 3612
Size: 913 1148 1551
Fragments arranged by size:
1551 1148 913
BstBI TT'CG_AA
Cuts at: 0 1554 3612
Size: 1554 2058
BstEII G'GTnAC_C
Cuts at: 0 3006 3612
Size: 3006 606
BstXI CCAn_nnnn'nTGG
Cuts at: 0 392 1384 2406 3324 3612
Size: 392 992 1022 918 288
Fragments arranged by size:
1022 992 918 392 288
BstYI r'GATC_y
Cuts at: 0 206 563 1359 1632 2133 3612
Size: 206 357 796 273 501 1479
Fragments arranged by size:
1479 796 501 357 273 206
BstZ17I GTA'TAC
Cuts at: 0 2175 3612
Size: 2175 1437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 1298 3612
Size: 19 1279 2314
Fragments arranged by size:
2314 1279 19
BtsI GCAGTG_nn'
Cuts at: 0 728 1510 1793 3187 3307 3612
Size: 728 782 283 1394 120 305
Fragments arranged by size:
1394 782 728 305 283 120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3585 3612
Size: 3585 27
DraI TTT'AAA
Cuts at: 0 120 3401 3612
Size: 120 3281 211
Fragments arranged by size:
3281 211 120
DraIII CAC_nnn'GTG
Cuts at: 0 2061 3612
Size: 2061 1551
DrdI GACnn_nn'nnGTC
Cuts at: 0 2479 3612
Size: 2479 1133
EaeI y'GGCC_r
Cuts at: 0 382 2027 2996 3593 3612
Size: 382 1645 969 597 19
Fragments arranged by size:
1645 969 597 382 19
EarI CTCTTCn'nnn_
Cuts at: 0 314 952 1110 1138 2104 2219 2707 3612
Size: 314 638 158 28 966 115 488 905
Fragments arranged by size:
966 905 638 488 314 158 115 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 891 3612
Size: 891 2721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 289 552 772 976 1119 1481 2149
Size: 187 102 263 220 204 143 362 668
Cuts at: 2149 2890 3346 3577 3612
Size: 741 456 231 35
Fragments arranged by size:
741 668 456 362 263 231 220 204
187 143 102 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1711 2224 2554 3132 3612
Size: 1711 513 330 578 480
Fragments arranged by size:
1711 578 513 480 330
EcoO109I rG'GnC_Cy
Cuts at: 0 1770 2563 2598 3612
Size: 1770 793 35 1014
Fragments arranged by size:
1770 1014 793 35
EcoRI G'AATT_C
Cuts at: 0 1437 3612
Size: 1437 2175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1242 3612
Size: 1242 2370
HaeII r_GCGC'y
Cuts at: 0 84 3082 3435 3612
Size: 84 2998 353 177
Fragments arranged by size:
2998 353 177 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 331 363 697 729 951 983
Size: 55 32 244 32 334 32 222 32
Cuts at: 983 3109 3141 3612
Size: 2126 32 471
Fragments arranged by size:
2126 471 334 244 222 55 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2634 2894 3612
Size: 2634 260 718
Fragments arranged by size:
2634 718 260
HindIII A'AGCT_T
Cuts at: 0 3394 3612
Size: 3394 218
HpaI GTT'AAC
Cuts at: 0 2894 3612
Size: 2894 718
KpnI G_GTAC'C
Cuts at: 0 3324 3612
Size: 3324 288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1434 3612
Size: 821 613 2178
Fragments arranged by size:
2178 821 613
MscI TGG'CCA
Cuts at: 0 384 2029 2998 3595 3612
Size: 384 1645 969 597 17
Fragments arranged by size:
1645 969 597 384 17
MslI CAynn'nnrTG
Cuts at: 0 1546 1828 2957 3065 3223 3612
Size: 1546 282 1129 108 158 389
Fragments arranged by size:
1546 1129 389 282 158 108
MspA1I CmG'CkG
Cuts at: 0 36 802 996 3612
Size: 36 766 194 2616
Fragments arranged by size:
2616 766 194 36
NdeI CA'TA_TG
Cuts at: 0 2771 3612
Size: 2771 841
NheI G'CTAG_C
Cuts at: 0 2373 3612
Size: 2373 1239
NsiI A_TGCA'T
Cuts at: 0 890 3612
Size: 890 2722
NspI r_CATG'y
Cuts at: 0 246 405 1352 1810 1875 2055 3612
Size: 246 159 947 458 65 180 1557
Fragments arranged by size:
1557 947 458 246 180 159 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 2036 2974 3612
Size: 555 1481 938 638
Fragments arranged by size:
1481 938 638 555
PfoI T'CCnGG_A
Cuts at: 0 209 749 3612
Size: 209 540 2863
Fragments arranged by size:
2863 540 209
PpuMI rG'GwC_Cy
Cuts at: 0 2598 3612
Size: 2598 1014
PsiI TTA'TAA
Cuts at: 0 1880 2338 3612
Size: 1880 458 1274
Fragments arranged by size:
1880 1274 458
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2334 2366 3612
Size: 2334 32 1246
Fragments arranged by size:
2334 1246 32
PstI C_TGCA'G
Cuts at: 0 1452 2841 3416 3612
Size: 1452 1389 575 196
Fragments arranged by size:
1452 1389 575 196
PvuII CAG'CTG
Cuts at: 0 36 996 3612
Size: 36 960 2616
Fragments arranged by size:
2616 960 36
SapI GCTCTTCn'nnn_
Cuts at: 0 1110 2104 2707 3612
Size: 1110 994 603 905
Fragments arranged by size:
1110 994 905 603
SfcI C'TryA_G
Cuts at: 0 1448 1692 2837 3412 3612
Size: 1448 244 1145 575 200
Fragments arranged by size:
1448 1145 575 244 200
SmaI CCC'GGG
Cuts at: 0 1783 3612
Size: 1783 1829
SmlI C'TyrA_G
Cuts at: 0 100 153 2045 2858 3612
Size: 100 53 1892 813 754
Fragments arranged by size:
1892 813 754 100 53
SphI G_CATG'C
Cuts at: 0 1810 3612
Size: 1810 1802
SspI AAT'ATT
Cuts at: 0 1261 2646 3612
Size: 1261 1385 966
Fragments arranged by size:
1385 1261 966
StuI AGG'CCT
Cuts at: 0 2126 3612
Size: 2126 1486
StyI C'CwwG_G
Cuts at: 0 1628 2399 2550 3482 3612
Size: 1628 771 151 932 130
Fragments arranged by size:
1628 932 771 151 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 699 1000 1558 2176 3612
Size: 91 608 301 558 618 1436
Fragments arranged by size:
1436 618 608 558 301 91
TatI w'GTAC_w
Cuts at: 0 3073 3612
Size: 3073 539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 745 777 1068 1100 2122 2154 2234 2266
Size: 745 32 291 32 1022 32 80 32
Cuts at: 2266 3612
Size: 1346
Fragments arranged by size:
1346 1022 745 291 80 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1209 3612
Size: 1209 2403
XmaI C'CCGG_G
Cuts at: 0 1781 3612
Size: 1781 1831
XmnI GAAnn'nnTTC
Cuts at: 0 1064 1574 1999 3612
Size: 1064 510 425 1613
Fragments arranged by size:
1613 1064 510 425
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI
ApaLI ApoI AseI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NdeI NheI NsiI NspI PflMI
PfoI PpuMI PsiI PsrI PstI PvuII SapI SfcI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AlfI AloI ApaI
AscI AsiSI BaeI BbeI BcgI BglII BmgBI BsaI
BsaHI BsiEI BsiWI BspEI BspMI BsrBI BssHII BssSI
Bsu36I BtgI ClaI EagI EcoICRI EcoRV FseI FspI
FspAI KasI MfeI MluI NaeI NarI NcoI NgoMIV
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI ZraI