Restriction map for
pF1KSDA1277
(Linear) (Six-base) MAPSORT of: KIAA1277.seq Check: 2945 from: 1 to: 3027
KIAA1277.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AatII G_ACGT'C
Cuts at: 0 2758 3027
Size: 2758 269
AccI GT'mk_AC
Cuts at: 0 2420 3027
Size: 2420 607
Acc65I G'GTAC_C
Cuts at: 0 1643 3027
Size: 1643 1384
AclI AA'CG_TT
Cuts at: 0 814 3027
Size: 814 2213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 849 1648 1795 1843 2004 2006 2566
Size: 499 350 799 147 48 161 2 560
Cuts at: 2566 2720 3007 3027
Size: 154 287 20
Fragments arranged by size:
799 560 499 350 287 161 154 147
48 20 2
AflIII A'CryG_T
Cuts at: 0 746 3027
Size: 746 2281
AhdI GACnn_n'nnGTC
Cuts at: 0 2229 3027
Size: 2229 798
AleI CACnn'nnGTG
Cuts at: 0 2969 3027
Size: 2969 58
AlwNI CAG_nnn'CTG
Cuts at: 0 1605 2173 3027
Size: 1605 568 854
Fragments arranged by size:
1605 854 568
ApaI G_GGCC'C
Cuts at: 0 2648 3027
Size: 2648 379
ApoI r'AATT_y
Cuts at: 0 1197 1278 3010 3027
Size: 1197 81 1732 17
Fragments arranged by size:
1732 1197 81 17
AvaI C'yCGr_G
Cuts at: 0 496 1658 2212 3027
Size: 496 1162 554 815
Fragments arranged by size:
1162 815 554 496
BamHI G'GATC_C
Cuts at: 0 405 531 3027
Size: 405 126 2496
Fragments arranged by size:
2496 405 126
BanI G'GyrC_C
Cuts at: 0 174 1635 1643 3027
Size: 174 1461 8 1384
Fragments arranged by size:
1461 1384 174 8
BanII G_rGCy'C
Cuts at: 0 680 760 2648 2931 3027
Size: 680 80 1888 283 96
Fragments arranged by size:
1888 680 283 96 80
BbeI G_GCGC'C
Cuts at: 0 1639 3027
Size: 1639 1388
BbsI GAAGACnn'nnnn_
Cuts at: 0 960 1019 1118 1956 2835 3027
Size: 960 59 99 838 879 192
Fragments arranged by size:
960 879 838 192 99 59
BbvCI CC'TCA_GC
Cuts at: 0 1811 2248 3027
Size: 1811 437 779
Fragments arranged by size:
1811 779 437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1621 1655 1917 1951 3027
Size: 1621 34 262 34 1076
Fragments arranged by size:
1621 1076 262 34 34
BclI T'GATC_A
Cuts at: 0 23 3027
Size: 23 3004
BglI GCCn_nnn'nGGC
Cuts at: 0 55 3027
Size: 55 2972
BlpI GC'TnA_GC
Cuts at: 0 2323 3027
Size: 2323 704
Bme1580I G_kGCm'C
Cuts at: 0 179 2648 3027
Size: 179 2469 379
Fragments arranged by size:
2469 379 179
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1346 1506 1830 2948 3027
Size: 531 815 160 324 1118 79
Fragments arranged by size:
1118 815 531 324 160 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1545 3027
Size: 1545 1482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1934 1982 2311 2448 2857 3027
Size: 1934 48 329 137 409 170
Fragments arranged by size:
1934 409 329 170 137 48
Bpu10I CC'TnA_GC
Cuts at: 0 382 686 1811 2248 2482 3027
Size: 382 304 1125 437 234 545
Fragments arranged by size:
1125 545 437 382 304 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 3027
Size: 760 2267
BsaI GGTCTCn'nnnn_
Cuts at: 0 1400 3027
Size: 1400 1627
BsaAI yAC'GTr
Cuts at: 0 1746 2220 3027
Size: 1746 474 807
Fragments arranged by size:
1746 807 474
BsaHI Gr'CG_yC
Cuts at: 0 1636 2440 2755 3027
Size: 1636 804 315 272
Fragments arranged by size:
1636 804 315 272
BsaWI w'CCGG_w
Cuts at: 0 731 1478 3027
Size: 731 747 1549
Fragments arranged by size:
1549 747 731
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 237 267 3027
Size: 237 30 2760
Fragments arranged by size:
2760 237 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 258 261 881 915 1111 1302 1591 2232
Size: 258 3 620 34 196 191 289 641
Cuts at: 2232 2310 2785 2802 3027
Size: 78 475 17 225
Fragments arranged by size:
641 620 475 289 258 225 196 191
78 34 17 3
BseYI C'CCAG_C
Cuts at: 0 36 58 190 413 1071 1372 2121 3027
Size: 36 22 132 223 658 301 749 906
Fragments arranged by size:
906 749 658 301 223 132 36 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1004 3027
Size: 170 834 2023
Fragments arranged by size:
2023 834 170
BsiHKAI G_wGCw'C
Cuts at: 0 316 2219 2434 2966 3027
Size: 316 1903 215 532 61
Fragments arranged by size:
1903 532 316 215 61
Bsp1286I G_dGCh'C
Cuts at: 0 179 316 680 760 2219 2434 2648 2931
Size: 179 137 364 80 1459 215 214 283
Cuts at: 2931 2966 3027
Size: 35 61
Fragments arranged by size:
1459 364 283 215 214 179 137 80
61 35
BspEI T'CCGG_A
Cuts at: 0 1478 3027
Size: 1478 1549
BspHI T'CATG_A
Cuts at: 0 1799 2093 3027
Size: 1799 294 934
Fragments arranged by size:
1799 934 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 1269 3027
Size: 474 795 1758
Fragments arranged by size:
1758 795 474
BsrBI CCG'CTC
Cuts at: 0 2883 3027
Size: 2883 144
BsrDI GCAATG_nn'
Cuts at: 0 2309 3027
Size: 2309 718
BsrFI r'CCGG_y
Cuts at: 0 2531 2894 3027
Size: 2531 363 133
Fragments arranged by size:
2531 363 133
BstYI r'GATC_y
Cuts at: 0 405 531 1122 2036 3027
Size: 405 126 591 914 991
Fragments arranged by size:
991 914 591 405 126
BtgI C'CryG_G
Cuts at: 0 2414 3027
Size: 2414 613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2365 3027
Size: 2365 662
BtsI GCAGTG_nn'
Cuts at: 0 347 564 2476 3027
Size: 347 217 1912 551
Fragments arranged by size:
1912 551 347 217
EarI CTCTTCn'nnn_
Cuts at: 0 1224 1423 1495 1696 2242 2495 3027
Size: 1224 199 72 201 546 253 532
Fragments arranged by size:
1224 546 532 253 201 199 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1115 2245 2815 3027
Size: 1115 1130 570 212
Fragments arranged by size:
1130 1115 570 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 849 1648 1795 1843 1934 1982 2004
Size: 499 350 799 147 48 91 48 22
Cuts at: 2004 2006 2311 2448 2566 2720 2857 3007 3027
Size: 2 305 137 118 154 137 150 20
Fragments arranged by size:
799 499 350 305 154 150 147 137
137 118 91 48 48 22 20 2
EcoO109I rG'GnC_Cy
Cuts at: 0 360 722 1340 2505 2644 2645 3027
Size: 360 362 618 1165 139 1 382
Fragments arranged by size:
1165 618 382 362 360 139 1
EcoRV GAT'ATC
Cuts at: 0 149 3027
Size: 149 2878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1209 2524 3027
Size: 468 741 1315 503
Fragments arranged by size:
1315 741 503 468
HaeII r_GCGC'y
Cuts at: 0 1605 1639 1980 2362 3027
Size: 1605 34 341 382 665
Fragments arranged by size:
1605 665 382 341 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 454 486 577 609 2051 2083
Size: 133 32 289 32 91 32 1442 32
Cuts at: 2083 2215 2247 2545 2577 2680 2712 3027
Size: 132 32 298 32 103 32 315
Fragments arranged by size:
1442 315 298 289 133 132 103 91
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1611 2421 2517 2526 3027
Size: 1611 810 96 9 501
Fragments arranged by size:
1611 810 501 96 9
HindIII A'AGCT_T
Cuts at: 0 2578 3027
Size: 2578 449
KasI G'GCGC_C
Cuts at: 0 1635 3027
Size: 1635 1392
KpnI G_GTAC'C
Cuts at: 0 1647 3027
Size: 1647 1380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1296 1760 3027
Size: 1296 464 1267
Fragments arranged by size:
1296 1267 464
MslI CAynn'nnrTG
Cuts at: 0 2398 2969 3027
Size: 2398 571 58
Fragments arranged by size:
2398 571 58
MspA1I CmG'CkG
Cuts at: 0 40 2199 2496 2787 2969 3027
Size: 40 2159 297 291 182 58
Fragments arranged by size:
2159 297 291 182 58 40
NaeI GCC'GGC
Cuts at: 0 2896 3027
Size: 2896 131
NarI GG'CG_CC
Cuts at: 0 1636 3027
Size: 1636 1391
NcoI C'CATG_G
Cuts at: 0 2414 3027
Size: 2414 613
NgoMIV G'CCGG_C
Cuts at: 0 2894 3027
Size: 2894 133
NspI r_CATG'y
Cuts at: 0 826 3027
Size: 826 2201
PasI CC'CwG_GG
Cuts at: 0 725 2127 3027
Size: 725 1402 900
Fragments arranged by size:
1402 900 725
PflMI CCAn_nnn'nTGG
Cuts at: 0 439 1518 1605 1862 2352 2835 3027
Size: 439 1079 87 257 490 483 192
Fragments arranged by size:
1079 490 483 439 257 192 87
PfoI T'CCnGG_A
Cuts at: 0 2591 2834 2876 3027
Size: 2591 243 42 151
Fragments arranged by size:
2591 243 151 42
PmlI CAC'GTG
Cuts at: 0 2220 3027
Size: 2220 807
PpuMI rG'GwC_Cy
Cuts at: 0 360 1340 2505 3027
Size: 360 980 1165 522
Fragments arranged by size:
1165 980 522 360
PspOMI G'GGCC_C
Cuts at: 0 2644 3027
Size: 2644 383
PspXI vC'TCGA_Gb
Cuts at: 0 496 2212 3027
Size: 496 1716 815
Fragments arranged by size:
1716 815 496
PstI C_TGCA'G
Cuts at: 0 45 1025 2786 3027
Size: 45 980 1761 241
Fragments arranged by size:
1761 980 241 45
PvuII CAG'CTG
Cuts at: 0 40 3027
Size: 40 2987
SalI G'TCGA_C
Cuts at: 0 2419 3027
Size: 2419 608
SanDI GG'GwC_CC
Cuts at: 0 1340 3027
Size: 1340 1687
SapI GCTCTTCn'nnn_
Cuts at: 0 1696 3027
Size: 1696 1331
ScaI AGT'ACT
Cuts at: 0 1699 3027
Size: 1699 1328
SexAI A'CCwGG_T
Cuts at: 0 2261 3027
Size: 2261 766
SfcI C'TryA_G
Cuts at: 0 41 1021 2782 3027
Size: 41 980 1761 245
Fragments arranged by size:
1761 980 245 41
SfoI GGC'GCC
Cuts at: 0 1637 3027
Size: 1637 1390
SmaI CCC'GGG
Cuts at: 0 1660 3027
Size: 1660 1367
SmlI C'TyrA_G
Cuts at: 0 496 739 2212 3027
Size: 496 243 1473 815
Fragments arranged by size:
1473 815 496 243
StuI AGG'CCT
Cuts at: 0 2794 3027
Size: 2794 233
StyI C'CwwG_G
Cuts at: 0 1401 1856 2414 3027
Size: 1401 455 558 613
Fragments arranged by size:
1401 613 558 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 1774 3027
Size: 204 1570 1253
Fragments arranged by size:
1570 1253 204
TatI w'GTAC_w
Cuts at: 0 1697 3027
Size: 1697 1330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 237 269 982 1014 2162 2194 3027
Size: 237 32 713 32 1148 32 833
Fragments arranged by size:
1148 833 713 237 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2521 3027
Size: 2521 506
XbaI T'CTAG_A
Cuts at: 0 1119 3027
Size: 1119 1908
XhoI C'TCGA_G
Cuts at: 0 496 2212 3027
Size: 496 1716 815
Fragments arranged by size:
1716 815 496
XmaI C'CCGG_G
Cuts at: 0 1658 3027
Size: 1658 1369
XmnI GAAnn'nnTTC
Cuts at: 0 1115 2600 3027
Size: 1115 1485 427
Fragments arranged by size:
1485 1115 427
ZraI GAC'GTC
Cuts at: 0 2756 3027
Size: 2756 271
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BstYI BtgI BtgZI BtsI
EarI EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SalI SanDI SapI
ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BciVI BfrBI BglII BmgBI
BmtI BsaBI BsiEI BsiWI BsmI BsmBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI
EcoRI FseI FspI FspAI HpaI MfeI MluI MscI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacI SacII
SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XcmI