Restriction map for
pF1KSDA1276
(Linear) (Six-base) MAPSORT of: KIAA1276.seq Check: 7576 from: 1 to: 3180
KIAA1276.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 3064 3180
Size: 3064 116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 898 1682 2704 2770 3180
Size: 877 21 784 1022 66 410
Fragments arranged by size:
1022 877 784 410 66 21
AflII C'TTAA_G
Cuts at: 0 910 3180
Size: 910 2270
AleI CACnn'nnGTG
Cuts at: 0 139 3180
Size: 139 3041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1046 1180 2240 3180
Size: 1046 134 1060 940
Fragments arranged by size:
1060 1046 940 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2926 2958 3180
Size: 2926 32 222
Fragments arranged by size:
2926 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 309 492 606 627 729 876 897 1540
Size: 309 183 114 21 102 147 21 643
Cuts at: 1540 1845 2181 2208 3180
Size: 305 336 27 972
Fragments arranged by size:
972 643 336 309 305 183 147 114
102 27 21 21
ApaI G_GGCC'C
Cuts at: 0 571 3180
Size: 571 2609
ApaLI G'TGCA_C
Cuts at: 0 1854 3180
Size: 1854 1326
ApoI r'AATT_y
Cuts at: 0 930 1473 1504 3180
Size: 930 543 31 1676
Fragments arranged by size:
1676 930 543 31
AseI AT'TA_AT
Cuts at: 0 2987 3180
Size: 2987 193
AvaI C'yCGr_G
Cuts at: 0 1975 2358 2710 2862 3180
Size: 1975 383 352 152 318
Fragments arranged by size:
1975 383 352 318 152
BamHI G'GATC_C
Cuts at: 0 1596 3180
Size: 1596 1584
BanI G'GyrC_C
Cuts at: 0 67 2237 3180
Size: 67 2170 943
Fragments arranged by size:
2170 943 67
BanII G_rGCy'C
Cuts at: 0 52 221 461 541 571 1744 1934 1998
Size: 52 169 240 80 30 1173 190 64
Cuts at: 1998 2111 2280 2420 3142 3180
Size: 113 169 140 722 38
Fragments arranged by size:
1173 722 240 190 169 169 140 113
80 64 52 38 30
BbeI G_GCGC'C
Cuts at: 0 71 3180
Size: 71 3109
BbsI GAAGACnn'nnnn_
Cuts at: 0 1045 1229 1304 3180
Size: 1045 184 75 1876
Fragments arranged by size:
1876 1045 184 75
BbvCI CC'TCA_GC
Cuts at: 0 176 400 1109 1541 2272 3180
Size: 176 224 709 432 731 908
Fragments arranged by size:
908 731 709 432 224 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 105 139 991 1025 3180
Size: 105 34 852 34 2155
Fragments arranged by size:
2155 852 105 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 649 796 2369 2548 2605 2647 3180
Size: 649 147 1573 179 57 42 533
Fragments arranged by size:
1573 649 533 179 147 57 42
BlpI GC'TnA_GC
Cuts at: 0 628 3180
Size: 628 2552
Bme1580I G_kGCm'C
Cuts at: 0 571 1141 1858 2240 2625 2912 3180
Size: 571 570 717 382 385 287 268
Fragments arranged by size:
717 571 570 385 382 287 268
BmgBI CAC'GTC
Cuts at: 0 2907 3180
Size: 2907 273
BmrI ACTGGGnnnn_n'
Cuts at: 0 1747 2810 3180
Size: 1747 1063 370
Fragments arranged by size:
1747 1063 370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 384 3180
Size: 384 2796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 205 331 343 400 426 469 475
Size: 96 109 126 12 57 26 43 6
Cuts at: 475 847 1453 1606 1867 1918 1927 2029 2062
Size: 372 606 153 261 51 9 102 33
Cuts at: 2062 2095 2125 2425 2545 2620 3180
Size: 33 30 300 120 75 560
Fragments arranged by size:
606 560 372 300 261 153 126 120
109 102 96 75 57 51 43 33
33 30 26 12 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 176 400 800 1109 1541 2272 3180
Size: 176 224 400 309 432 731 908
Fragments arranged by size:
908 731 432 400 309 224 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1114 1504 1693 1873 2017 3180
Size: 445 669 390 189 180 144 1163
Fragments arranged by size:
1163 669 445 390 189 180 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 815 1582 1830 1933 2501 3180
Size: 232 583 767 248 103 568 679
Fragments arranged by size:
767 679 583 568 248 232 103
BsaAI yAC'GTr
Cuts at: 0 105 3180
Size: 105 3075
BsaHI Gr'CG_yC
Cuts at: 0 68 3180
Size: 68 3112
BsaWI w'CCGG_w
Cuts at: 0 806 2582 2677 2831 3180
Size: 806 1776 95 154 349
Fragments arranged by size:
1776 806 349 154 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 736 766 2093 2123 2926 2956 3180
Size: 736 30 1327 30 803 30 224
Fragments arranged by size:
1327 803 736 224 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 292 361 492 670 1270 1646 1651
Size: 229 63 69 131 178 600 376 5
Cuts at: 1651 1798 2164 2359 2440 2542 3180
Size: 147 366 195 81 102 638
Fragments arranged by size:
638 600 376 366 229 195 178 147
131 102 81 69 63 5
BseYI C'CCAG_C
Cuts at: 0 128 1970 2389 2543 2630 2911 3013 3031
Size: 128 1842 419 154 87 281 102 18
Cuts at: 3031 3125 3180
Size: 94 55
Fragments arranged by size:
1842 419 281 154 128 102 94 87
55 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 619 925 3180
Size: 393 226 306 2255
Fragments arranged by size:
2255 393 306 226
BsiHKAI G_wGCw'C
Cuts at: 0 221 419 461 833 1858 1934 2111 3180
Size: 221 198 42 372 1025 76 177 1069
Fragments arranged by size:
1069 1025 372 221 198 177 76 42
BsmI GAATG_Cn'
Cuts at: 0 231 1580 2809 3180
Size: 231 1349 1229 371
Fragments arranged by size:
1349 1229 371 231
BsmBI CGTCTCn'nnnn_
Cuts at: 0 376 3180
Size: 376 2804
Bsp1286I G_dGCh'C
Cuts at: 0 52 221 419 461 541 571 833 1141
Size: 52 169 198 42 80 30 262 308
Cuts at: 1141 1744 1858 1934 1998 2111 2240 2280 2420
Size: 603 114 76 64 113 129 40 140
Cuts at: 2420 2625 2912 3142 3180
Size: 205 287 230 38
Fragments arranged by size:
603 308 287 262 230 205 198 169
140 129 114 113 80 76 64 52
42 40 38 30
BspEI T'CCGG_A
Cuts at: 0 806 2677 3180
Size: 806 1871 503
Fragments arranged by size:
1871 806 503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1886 2507 3180
Size: 1886 621 673
Fragments arranged by size:
1886 673 621
BsrFI r'CCGG_y
Cuts at: 0 70 521 1093 2368 3180
Size: 70 451 572 1275 812
Fragments arranged by size:
1275 812 572 451 70
BssHII G'CGCG_C
Cuts at: 0 2463 3180
Size: 2463 717
BssSI C'ACGA_G
Cuts at: 0 369 502 3180
Size: 369 133 2678
Fragments arranged by size:
2678 369 133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 606 705 2142 2181 3079 3180
Size: 606 99 1437 39 898 101
Fragments arranged by size:
1437 898 606 101 99 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2559 2919 3180
Size: 2559 360 261
Fragments arranged by size:
2559 360 261
BstYI r'GATC_y
Cuts at: 0 1596 2081 3180
Size: 1596 485 1099
Fragments arranged by size:
1596 1099 485
BtgI C'CryG_G
Cuts at: 0 1319 1346 2600 3180
Size: 1319 27 1254 580
Fragments arranged by size:
1319 1254 580 27
BtsI GCAGTG_nn'
Cuts at: 0 742 947 1572 1683 1858 2257 2566 3180
Size: 742 205 625 111 175 399 309 614
Fragments arranged by size:
742 625 614 399 309 205 175 111
DraIII CAC_nnn'GTG
Cuts at: 0 1320 3180
Size: 1320 1860
EaeI y'GGCC_r
Cuts at: 0 2730 3039 3180
Size: 2730 309 141
Fragments arranged by size:
2730 309 141
EarI CTCTTCn'nnn_
Cuts at: 0 328 1070 1247 1436 2780 3180
Size: 328 742 177 189 1344 400
Fragments arranged by size:
1344 742 400 328 189 177
EcoICRI GAG'CTC
Cuts at: 0 219 459 1932 2109 3180
Size: 219 240 1473 177 1071
Fragments arranged by size:
1473 1071 240 219 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 205 331 343 400 426 469 475
Size: 96 109 126 12 57 26 43 6
Cuts at: 475 847 877 898 1453 1606 1682 1867 1918
Size: 372 30 21 555 153 76 185 51
Cuts at: 1918 1927 2029 2062 2095 2125 2425 2545 2620
Size: 9 102 33 33 30 300 120 75
Cuts at: 2620 2704 2770 3180
Size: 84 66 410
Fragments arranged by size:
555 410 372 300 185 153 126 120
109 102 96 84 76 75 66 57
51 43 33 33 30 30 26 21
12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 372 592 675 3180
Size: 372 220 83 2505
Fragments arranged by size:
2505 372 220 83
EcoO109I rG'GnC_Cy
Cuts at: 0 306 567 585 726 1875 2178 2715 3089
Size: 306 261 18 141 1149 303 537 374
Cuts at: 3089 3180
Size: 91
Fragments arranged by size:
1149 537 374 306 303 261 141 91
18
EcoRI G'AATT_C
Cuts at: 0 930 1473 3180
Size: 930 543 1707
Fragments arranged by size:
1707 930 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1243 3180
Size: 1243 1937
FspI TGC'GCA
Cuts at: 0 273 2002 3180
Size: 273 1729 1178
Fragments arranged by size:
1729 1178 273
HaeII r_GCGC'y
Cuts at: 0 71 196 320 355 2387 3180
Size: 71 125 124 35 2032 793
Fragments arranged by size:
2032 793 125 124 71 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 1581 1613 1747 1779 2093 2125
Size: 736 32 813 32 134 32 314 32
Cuts at: 2125 2402 2434 2750 2782 2955 2987 3180
Size: 277 32 316 32 173 32 193
Fragments arranged by size:
813 736 316 314 277 193 173 134
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 67 3180
Size: 67 3113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 734 1373 1536 2109 2192 3180
Size: 533 201 639 163 573 83 988
Fragments arranged by size:
988 639 573 533 201 163 83
MslI CAynn'nnrTG
Cuts at: 0 139 899 1162 3180
Size: 139 760 263 2018
Fragments arranged by size:
2018 760 263 139
MspA1I CmG'CkG
Cuts at: 0 603 627 1434 1660 2547 3035 3180
Size: 603 24 807 226 887 488 145
Fragments arranged by size:
887 807 603 488 226 145 24
NaeI GCC'GGC
Cuts at: 0 72 523 1095 2370 3180
Size: 72 451 572 1275 810
Fragments arranged by size:
1275 810 572 451 72
NarI GG'CG_CC
Cuts at: 0 68 3180
Size: 68 3112
NcoI C'CATG_G
Cuts at: 0 2600 3180
Size: 2600 580
NgoMIV G'CCGG_C
Cuts at: 0 70 521 1093 2368 3180
Size: 70 451 572 1275 812
Fragments arranged by size:
1275 812 572 451 70
NspI r_CATG'y
Cuts at: 0 2007 3180
Size: 2007 1173
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 2118 3180
Size: 309 1809 1062
Fragments arranged by size:
1809 1062 309
PfoI T'CCnGG_A
Cuts at: 0 779 1934 2310 2441 2522 2980 3180
Size: 779 1155 376 131 81 458 200
Fragments arranged by size:
1155 779 458 376 200 131 81
PmlI CAC'GTG
Cuts at: 0 105 3180
Size: 105 3075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2926 2958 3180
Size: 2926 32 222
Fragments arranged by size:
2926 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 585 1875 2715 3089 3180
Size: 585 1290 840 374 91
Fragments arranged by size:
1290 840 585 374 91
PspOMI G'GGCC_C
Cuts at: 0 567 3180
Size: 567 2613
PspXI vC'TCGA_Gb
Cuts at: 0 1975 3180
Size: 1975 1205
PstI C_TGCA'G
Cuts at: 0 260 332 665 797 1004 1373 1579 1883
Size: 260 72 333 132 207 369 206 304
Cuts at: 1883 1925 2147 2264 2741 3180
Size: 42 222 117 477 439
Fragments arranged by size:
477 439 369 333 304 260 222 207
206 132 117 72 42
PvuII CAG'CTG
Cuts at: 0 627 1434 1660 3035 3180
Size: 627 807 226 1375 145
Fragments arranged by size:
1375 807 627 226 145
SacI G_AGCT'C
Cuts at: 0 221 461 1934 2111 3180
Size: 221 240 1473 177 1069
Fragments arranged by size:
1473 1069 240 221 177
SbfI CC_TGCA'GG
Cuts at: 0 260 797 1883 3180
Size: 260 537 1086 1297
Fragments arranged by size:
1297 1086 537 260
ScaI AGT'ACT
Cuts at: 0 3169 3180
Size: 3169 11
SfcI C'TryA_G
Cuts at: 0 256 292 328 661 793 1000 1369 1575
Size: 256 36 36 333 132 207 369 206
Cuts at: 1575 1866 1879 1921 2143 2260 2737 3065 3180
Size: 291 13 42 222 117 477 328 115
Fragments arranged by size:
477 369 333 328 291 256 222 207
206 132 117 115 42 36 36 13
SfoI GGC'GCC
Cuts at: 0 69 3180
Size: 69 3111
SmaI CCC'GGG
Cuts at: 0 2360 2864 3180
Size: 2360 504 316
Fragments arranged by size:
2360 504 316
SmlI C'TyrA_G
Cuts at: 0 460 910 1129 1483 1708 1888 1975 2032
Size: 460 450 219 354 225 180 87 57
Cuts at: 2032 3180
Size: 1148
Fragments arranged by size:
1148 460 450 354 225 219 180 87
57
SphI G_CATG'C
Cuts at: 0 2007 3180
Size: 2007 1173
SrfI GCCC'GGGC
Cuts at: 0 2360 3180
Size: 2360 820
StuI AGG'CCT
Cuts at: 0 2116 3180
Size: 2116 1064
StyI C'CwwG_G
Cuts at: 0 137 242 2117 2600 3180
Size: 137 105 1875 483 580
Fragments arranged by size:
1875 580 483 137 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2828 3027 3180
Size: 2828 199 153
Fragments arranged by size:
2828 199 153
TatI w'GTAC_w
Cuts at: 0 3167 3180
Size: 3167 13
XbaI T'CTAG_A
Cuts at: 0 1779 3180
Size: 1779 1401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 3180
Size: 450 2730
XhoI C'TCGA_G
Cuts at: 0 1975 3180
Size: 1975 1205
XmaI C'CCGG_G
Cuts at: 0 2358 2862 3180
Size: 2358 504 318
Fragments arranged by size:
2358 504 318
XmnI GAAnn'nnTTC
Cuts at: 0 287 1226 3180
Size: 287 939 1954
Fragments arranged by size:
1954 939 287
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI
BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SbfI ScaI SfcI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AscI AsiSI AvrII BaeI BciVI BclI BfrBI BglII
BmtI BsaBI BsiEI BsiWI BspHI BsrBI BsrDI BsrGI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SexAI
SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TstI
Tth111I ZraI