Restriction map for
pF1KA1265
(Linear) (Six-base) MAPSORT of: KIAA1265.seq Check: 1792 from: 1 to: 2493
KIAA1265.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 2198 2493
Size: 2198 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1208 1739 2028 2392 2493
Size: 119 1089 531 289 364 101
Fragments arranged by size:
1089 531 364 289 119 101
AfeI AGC'GCT
Cuts at: 0 104 2493
Size: 104 2389
AflIII A'CryG_T
Cuts at: 0 942 2493
Size: 942 1551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1534 2493
Size: 1534 959
AlwNI CAG_nnn'CTG
Cuts at: 0 1666 2299 2493
Size: 1666 633 194
Fragments arranged by size:
1666 633 194
ApoI r'AATT_y
Cuts at: 0 22 148 405 726 1309 2412 2493
Size: 22 126 257 321 583 1103 81
Fragments arranged by size:
1103 583 321 257 126 81 22
AseI AT'TA_AT
Cuts at: 0 296 2493
Size: 296 2197
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 416 449 2493
Size: 416 33 2044
Fragments arranged by size:
2044 416 33
BamHI G'GATC_C
Cuts at: 0 1972 2493
Size: 1972 521
BanI G'GyrC_C
Cuts at: 0 1048 1928 2493
Size: 1048 880 565
Fragments arranged by size:
1048 880 565
BanII G_rGCy'C
Cuts at: 0 2057 2493
Size: 2057 436
BbsI GAAGACnn'nnnn_
Cuts at: 0 2109 2493
Size: 2109 384
BbvCI CC'TCA_GC
Cuts at: 0 1906 2493
Size: 1906 587
BciVI GTATCCnnnnn_n'
Cuts at: 0 444 2493
Size: 444 2049
BclI T'GATC_A
Cuts at: 0 564 765 1401 2018 2493
Size: 564 201 636 617 475
Fragments arranged by size:
636 617 564 475 201
BfrBI ATG'CAT
Cuts at: 0 513 2493
Size: 513 1980
BglI GCCn_nnn'nGGC
Cuts at: 0 2221 2493
Size: 2221 272
BglII A'GATC_T
Cuts at: 0 885 978 2493
Size: 885 93 1515
Fragments arranged by size:
1515 885 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 602 2493
Size: 602 1891
Bpu10I CC'TnA_GC
Cuts at: 0 1906 2493
Size: 1906 587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1666 2493
Size: 1666 827
BsaI GGTCTCn'nnnn_
Cuts at: 0 640 2493
Size: 640 1853
BsaBI GATnn'nnATC
Cuts at: 0 890 995 2017 2493
Size: 890 105 1022 476
Fragments arranged by size:
1022 890 476 105
BsaHI Gr'CG_yC
Cuts at: 0 1361 2493
Size: 1361 1132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 702 732 2493
Size: 702 30 1761
Fragments arranged by size:
1761 702 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1816 2197 2493
Size: 1816 381 296
Fragments arranged by size:
1816 381 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 2087 2493
Size: 1856 231 406
Fragments arranged by size:
1856 406 231
BsmI GAATG_Cn'
Cuts at: 0 387 1938 2493
Size: 387 1551 555
Fragments arranged by size:
1551 555 387
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1352 2493
Size: 1352 1141
Bsp1286I G_dGCh'C
Cuts at: 0 2057 2493
Size: 2057 436
BspHI T'CATG_A
Cuts at: 0 300 861 1002 1848 2124 2493
Size: 300 561 141 846 276 369
Fragments arranged by size:
846 561 369 300 276 141
BsrDI GCAATG_nn'
Cuts at: 0 60 69 636 2493
Size: 60 9 567 1857
Fragments arranged by size:
1857 567 60 9
BsrGI T'GTAC_A
Cuts at: 0 813 837 2493
Size: 813 24 1656
Fragments arranged by size:
1656 813 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 71 2065 2299 2493
Size: 71 1994 234 194
Fragments arranged by size:
1994 234 194 71
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2445 2493
Size: 2445 48
BstYI r'GATC_y
Cuts at: 0 306 885 978 1972 2285 2493
Size: 306 579 93 994 313 208
Fragments arranged by size:
994 579 313 306 208 93
BstZ17I GTA'TAC
Cuts at: 0 2199 2493
Size: 2199 294
BtgI C'CryG_G
Cuts at: 0 90 1884 1953 2493
Size: 90 1794 69 540
Fragments arranged by size:
1794 540 90 69
BtsI GCAGTG_nn'
Cuts at: 0 1536 2297 2493
Size: 1536 761 196
Fragments arranged by size:
1536 761 196
DraI TTT'AAA
Cuts at: 0 404 2493
Size: 404 2089
EaeI y'GGCC_r
Cuts at: 0 312 2493
Size: 312 2181
EarI CTCTTCn'nnn_
Cuts at: 0 1793 2493
Size: 1793 700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1208 1739 2028 2392 2493
Size: 119 1089 531 289 364 101
Fragments arranged by size:
1089 531 364 289 119 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 928 1744 2493
Size: 928 816 749
Fragments arranged by size:
928 816 749
HaeII r_GCGC'y
Cuts at: 0 106 2493
Size: 106 2387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 963 995 2493
Size: 963 32 1498
Fragments arranged by size:
1498 963 32
HindIII A'AGCT_T
Cuts at: 0 1630 2493
Size: 1630 863
MfeI C'AATT_G
Cuts at: 0 2060 2192 2493
Size: 2060 132 301
Fragments arranged by size:
2060 301 132
MscI TGG'CCA
Cuts at: 0 314 2493
Size: 314 2179
MslI CAynn'nnrTG
Cuts at: 0 74 89 320 455 512 833 1001 1415
Size: 74 15 231 135 57 321 168 414
Cuts at: 1415 1421 1427 1439 1445 1546 1829 1853 1952
Size: 6 6 12 6 101 283 24 99
Cuts at: 1952 2233 2357 2493
Size: 281 124 136
Fragments arranged by size:
414 321 283 281 231 168 136 135
124 101 99 74 57 24 15 12
6 6 6
MspA1I CmG'CkG
Cuts at: 0 2251 2493
Size: 2251 242
NcoI C'CATG_G
Cuts at: 0 90 1953 2493
Size: 90 1863 540
Fragments arranged by size:
1863 540 90
NdeI CA'TA_TG
Cuts at: 0 11 509 2493
Size: 11 498 1984
Fragments arranged by size:
1984 498 11
NsiI A_TGCA'T
Cuts at: 0 515 2493
Size: 515 1978
NspI r_CATG'y
Cuts at: 0 946 1420 2238 2310 2493
Size: 946 474 818 72 183
Fragments arranged by size:
946 818 474 183 72
PciI A'CATG_T
Cuts at: 0 942 2493
Size: 942 1551
PstI C_TGCA'G
Cuts at: 0 2304 2493
Size: 2304 189
PvuII CAG'CTG
Cuts at: 0 2251 2493
Size: 2251 242
ScaI AGT'ACT
Cuts at: 0 2103 2493
Size: 2103 390
SexAI A'CCwGG_T
Cuts at: 0 744 1178 2493
Size: 744 434 1315
Fragments arranged by size:
1315 744 434
SfcI C'TryA_G
Cuts at: 0 1340 2108 2300 2493
Size: 1340 768 192 193
Fragments arranged by size:
1340 768 193 192
SmlI C'TyrA_G
Cuts at: 0 1681 2493
Size: 1681 812
SphI G_CATG'C
Cuts at: 0 2238 2493
Size: 2238 255
StyI C'CwwG_G
Cuts at: 0 90 270 1296 1953 2324 2493
Size: 90 180 1026 657 371 169
Fragments arranged by size:
1026 657 371 180 169 90
SwaI ATTT'AAAT
Cuts at: 0 404 2493
Size: 404 2089
TatI w'GTAC_w
Cuts at: 0 813 837 2101 2493
Size: 813 24 1264 392
Fragments arranged by size:
1264 813 392 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 2493
Size: 373 32 2088
Fragments arranged by size:
2088 373 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1390 1420 1667 2493
Size: 1390 30 247 826
Fragments arranged by size:
1390 826 247 30
XmnI GAAnn'nnTTC
Cuts at: 0 104 1632 2493
Size: 104 1528 861
Fragments arranged by size:
1528 861 104
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AlwNI ApoI AseI
BaeI BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglI BglII BmrI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BsgI BsmI BsmBI Bsp1286I BspHI
BsrDI BsrGI BstAPI BstXI BstYI BstZ17I BtgI BtsI
DraI EaeI EarI Eco57MI FalI HaeII Hin4I HindIII
MfeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PstI PvuII ScaI SexAI SfcI SmlI SphI
StyI SwaI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BbeI
BcgI BlpI Bme1580I BmgBI BmtI BplI BpmI BsaAI
BsaWI BseYI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
TaqII Tth111I XbaI XhoI XmaI ZraI