Restriction map for
pF1KSDA1257
(Linear) (Six-base) MAPSORT of: KIAA1257.seq Check: 7347 from: 1 to: 1227
KIAA1257.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
Acc65I G'GTAC_C
Cuts at: 0 1168 1227
Size: 1168 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1227
Size: 130 1097
AfeI AGC'GCT
Cuts at: 0 1128 1227
Size: 1128 99
AhdI GACnn_n'nnGTC
Cuts at: 0 591 1227
Size: 591 636
AleI CACnn'nnGTG
Cuts at: 0 473 1227
Size: 473 754
AlwNI CAG_nnn'CTG
Cuts at: 0 1200 1227
Size: 1200 27
ApaI G_GGCC'C
Cuts at: 0 129 1227
Size: 129 1098
ApoI r'AATT_y
Cuts at: 0 226 546 1227
Size: 226 320 681
Fragments arranged by size:
681 320 226
AseI AT'TA_AT
Cuts at: 0 961 1227
Size: 961 266
BanI G'GyrC_C
Cuts at: 0 1168 1227
Size: 1168 59
BanII G_rGCy'C
Cuts at: 0 50 129 827 1227
Size: 50 79 698 400
Fragments arranged by size:
698 400 79 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 144 178 1227
Size: 144 34 1049
Fragments arranged by size:
1049 144 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1221 1227
Size: 1221 6
Bme1580I G_kGCm'C
Cuts at: 0 129 209 1227
Size: 129 80 1018
Fragments arranged by size:
1018 129 80
BmgBI CAC'GTC
Cuts at: 0 96 204 645 1227
Size: 96 108 441 582
Fragments arranged by size:
582 441 108 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 69 156 1227
Size: 69 87 1071
Fragments arranged by size:
1071 87 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1227
Size: 457 770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1227
Size: 585 642
BsaI GGTCTCn'nnnn_
Cuts at: 0 1019 1227
Size: 1019 208
BsaAI yAC'GTr
Cuts at: 0 213 1227
Size: 213 1014
BsaBI GATnn'nnATC
Cuts at: 0 949 1227
Size: 949 278
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 1074 1227
Size: 45 1029 153
Fragments arranged by size:
1029 153 45
BseYI C'CCAG_C
Cuts at: 0 1190 1227
Size: 1190 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 1227
Size: 174 1053
Bsp1286I G_dGCh'C
Cuts at: 0 50 129 209 827 1227
Size: 50 79 80 618 400
Fragments arranged by size:
618 400 80 79 50
BsrBI CCG'CTC
Cuts at: 0 1158 1227
Size: 1158 69
BsrFI r'CCGG_y
Cuts at: 0 628 1165 1227
Size: 628 537 62
Fragments arranged by size:
628 537 62
BssSI C'ACGA_G
Cuts at: 0 81 1227
Size: 81 1146
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1200 1227
Size: 1200 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 927 1143 1227
Size: 240 687 216 84
Fragments arranged by size:
687 240 216 84
BtgI C'CryG_G
Cuts at: 0 463 1227
Size: 463 764
BtsI GCAGTG_nn'
Cuts at: 0 1173 1227
Size: 1173 54
DraI TTT'AAA
Cuts at: 0 686 1227
Size: 686 541
EaeI y'GGCC_r
Cuts at: 0 422 1145 1213 1222 1227
Size: 422 723 68 9 5
Fragments arranged by size:
723 422 68 9 5
EarI CTCTTCn'nnn_
Cuts at: 0 20 734 1082 1227
Size: 20 714 348 145
Fragments arranged by size:
714 348 145 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 749 1227
Size: 749 478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 457 1227
Size: 130 327 770
Fragments arranged by size:
770 327 130
EcoO109I rG'GnC_Cy
Cuts at: 0 30 125 1111 1227
Size: 30 95 986 116
Fragments arranged by size:
986 116 95 30
HaeII r_GCGC'y
Cuts at: 0 1130 1227
Size: 1130 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 577 609 958 990 1227
Size: 157 32 388 32 349 32 237
Fragments arranged by size:
388 349 237 157 32 32 32
HincII GTy'rAC
Cuts at: 0 399 1227
Size: 399 828
KpnI G_GTAC'C
Cuts at: 0 1172 1227
Size: 1172 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1227
Size: 224 1003
MscI TGG'CCA
Cuts at: 0 424 1215 1227
Size: 424 791 12
Fragments arranged by size:
791 424 12
MslI CAynn'nnrTG
Cuts at: 0 473 1227
Size: 473 754
MspA1I CmG'CkG
Cuts at: 0 1143 1227
Size: 1143 84
NaeI GCC'GGC
Cuts at: 0 630 1227
Size: 630 597
NgoMIV G'CCGG_C
Cuts at: 0 628 1227
Size: 628 599
NspI r_CATG'y
Cuts at: 0 13 1227
Size: 13 1214
PfoI T'CCnGG_A
Cuts at: 0 434 1227
Size: 434 793
PmlI CAC'GTG
Cuts at: 0 213 1227
Size: 213 1014
PpuMI rG'GwC_Cy
Cuts at: 0 30 1227
Size: 30 1197
PshAI GACnn'nnGTC
Cuts at: 0 1011 1227
Size: 1011 216
PspOMI G'GGCC_C
Cuts at: 0 125 1227
Size: 125 1102
PvuII CAG'CTG
Cuts at: 0 1143 1227
Size: 1143 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1221 1227
Size: 1221 6
SmlI C'TyrA_G
Cuts at: 0 564 1227
Size: 564 663
StyI C'CwwG_G
Cuts at: 0 116 1227
Size: 116 1111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 806 1227
Size: 477 329 421
Fragments arranged by size:
477 421 329
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 1227
Size: 230 32 965
Fragments arranged by size:
965 230 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 1227
Size: 432 795
Enzymes that do cut:
Acc65I AcuI AfeI AhdI AleI AlwNI ApaI ApoI
AseI BanI BanII BcgI BglI Bme1580I BmgBI BplI
BpmI BpuEI BsaI BsaAI BsaBI BseRI BseYI BsgI
Bsp1286I BsrBI BsrFI BssSI BstAPI BstXI BtgI BtsI
DraI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PfoI PmlI PpuMI PshAI PspOMI PvuII SfiI
SmlI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AlfI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BmtI Bpu10I BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI