Restriction map for
pF1KA1256
(Linear) (Six-base) MAPSORT of: KIAA1256.seq Check: 2433 from: 1 to: 5010
KIAA1256.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3297 5010
Size: 3297 1713
AccI GT'mk_AC
Cuts at: 0 612 4481 5010
Size: 612 3869 529
Fragments arranged by size:
3869 612 529
Acc65I G'GTAC_C
Cuts at: 0 1662 5010
Size: 1662 3348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 89 283 416 802 1510 1615 3086
Size: 80 9 194 133 386 708 105 1471
Cuts at: 3086 3586 3677 3742 4598 5010
Size: 500 91 65 856 412
Fragments arranged by size:
1471 856 708 500 412 386 194 133
105 91 80 65 9
AflIII A'CryG_T
Cuts at: 0 41 5010
Size: 41 4969
AlwNI CAG_nnn'CTG
Cuts at: 0 630 2488 3830 5010
Size: 630 1858 1342 1180
Fragments arranged by size:
1858 1342 1180 630
ApaI G_GGCC'C
Cuts at: 0 4214 5010
Size: 4214 796
ApoI r'AATT_y
Cuts at: 0 751 1391 2099 2328 2999 3898 4767 4874
Size: 751 640 708 229 671 899 869 107
Cuts at: 4874 4889 5010
Size: 15 121
Fragments arranged by size:
899 869 751 708 671 640 229 121
107 15
AseI AT'TA_AT
Cuts at: 0 334 1019 3160 3380 4505 5010
Size: 334 685 2141 220 1125 505
Fragments arranged by size:
2141 1125 685 505 334 220
AvaI C'yCGr_G
Cuts at: 0 4063 5010
Size: 4063 947
BamHI G'GATC_C
Cuts at: 0 4139 5010
Size: 4139 871
BanI G'GyrC_C
Cuts at: 0 1662 4625 5010
Size: 1662 2963 385
Fragments arranged by size:
2963 1662 385
BanII G_rGCy'C
Cuts at: 0 3356 3698 4214 4717 5010
Size: 3356 342 516 503 293
Fragments arranged by size:
3356 516 503 342 293
BbeI G_GCGC'C
Cuts at: 0 4629 5010
Size: 4629 381
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 3751 3877 3887 4446 5010
Size: 413 3338 126 10 559 564
Fragments arranged by size:
3338 564 559 413 126 10
BbvCI CC'TCA_GC
Cuts at: 0 383 722 2123 2162 5010
Size: 383 339 1401 39 2848
Fragments arranged by size:
2848 1401 383 339 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 2142 5010
Size: 2142 2868
BclI T'GATC_A
Cuts at: 0 126 5010
Size: 126 4884
BglII A'GATC_T
Cuts at: 0 1511 5010
Size: 1511 3499
BlpI GC'TnA_GC
Cuts at: 0 3797 5010
Size: 3797 1213
Bme1580I G_kGCm'C
Cuts at: 0 4214 5010
Size: 4214 796
BmgBI CAC'GTC
Cuts at: 0 4809 5010
Size: 4809 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 707 5010
Size: 707 4303
BmtI G_CTAG'C
Cuts at: 0 3056 5010
Size: 3056 1954
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4101 5010
Size: 4101 909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 2059 2279 2930 3083 3933 4033 4153
Size: 380 1679 220 651 153 850 100 120
Cuts at: 4153 5010
Size: 857
Fragments arranged by size:
1679 857 850 651 380 220 153 120
100
Bpu10I CC'TnA_GC
Cuts at: 0 383 722 971 2123 2162 3092 5010
Size: 383 339 249 1152 39 930 1918
Fragments arranged by size:
1918 1152 930 383 339 249 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 4073 5010
Size: 1498 2575 937
Fragments arranged by size:
2575 1498 937
BsaI GGTCTCn'nnnn_
Cuts at: 0 3445 4829 5010
Size: 3445 1384 181
Fragments arranged by size:
3445 1384 181
BsaAI yAC'GTr
Cuts at: 0 136 4152 5010
Size: 136 4016 858
Fragments arranged by size:
4016 858 136
BsaBI GATnn'nnATC
Cuts at: 0 2580 5010
Size: 2580 2430
BsaHI Gr'CG_yC
Cuts at: 0 1143 4543 4626 5010
Size: 1143 3400 83 384
Fragments arranged by size:
3400 1143 384 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 951 981 4030 4060 5010
Size: 951 30 3049 30 950
Fragments arranged by size:
3049 951 950 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1010 1072 2140 2179 4090 5010
Size: 1010 62 1068 39 1911 920
Fragments arranged by size:
1911 1068 1010 920 62 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 3790 4174 4252 4546 5010
Size: 386 3404 384 78 294 464
Fragments arranged by size:
3404 464 386 384 294 78
BsiHKAI G_wGCw'C
Cuts at: 0 3356 3698 4087 5010
Size: 3356 342 389 923
Fragments arranged by size:
3356 923 389 342
BsmI GAATG_Cn'
Cuts at: 0 3944 5010
Size: 3944 1066
Bsp1286I G_dGCh'C
Cuts at: 0 3356 3698 4087 4214 4717 5010
Size: 3356 342 389 127 503 293
Fragments arranged by size:
3356 503 389 342 293 127
BspHI T'CATG_A
Cuts at: 0 3561 5010
Size: 3561 1449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3297 4988 5010
Size: 3297 1691 22
Fragments arranged by size:
3297 1691 22
BsrBI CCG'CTC
Cuts at: 0 3585 4068 4601 5010
Size: 3585 483 533 409
Fragments arranged by size:
3585 533 483 409
BsrDI GCAATG_nn'
Cuts at: 0 388 556 920 5010
Size: 388 168 364 4090
Fragments arranged by size:
4090 388 364 168
BsrFI r'CCGG_y
Cuts at: 0 162 3765 5010
Size: 162 3603 1245
Fragments arranged by size:
3603 1245 162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3112 5010
Size: 3112 1898
BstBI TT'CG_AA
Cuts at: 0 4893 5010
Size: 4893 117
BstEII G'GTnAC_C
Cuts at: 0 2946 5010
Size: 2946 2064
BstXI CCAn_nnnn'nTGG
Cuts at: 0 44 2959 3847 4206 5010
Size: 44 2915 888 359 804
Fragments arranged by size:
2915 888 804 359 44
BstYI r'GATC_y
Cuts at: 0 643 1511 2575 3480 4139 4794 5010
Size: 643 868 1064 905 659 655 216
Fragments arranged by size:
1064 905 868 659 655 643 216
BstZ17I GTA'TAC
Cuts at: 0 613 5010
Size: 613 4397
BtgI C'CryG_G
Cuts at: 0 3925 5010
Size: 3925 1085
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4457 5010
Size: 4457 553
BtsI GCAGTG_nn'
Cuts at: 0 1591 3991 4162 5010
Size: 1591 2400 171 848
Fragments arranged by size:
2400 1591 848 171
DraI TTT'AAA
Cuts at: 0 3905 5010
Size: 3905 1105
EaeI y'GGCC_r
Cuts at: 0 932 3791 5010
Size: 932 2859 1219
Fragments arranged by size:
2859 1219 932
EarI CTCTTCn'nnn_
Cuts at: 0 893 1049 1199 2102 2739 3575 4285 5010
Size: 893 156 150 903 637 836 710 725
Fragments arranged by size:
903 893 836 725 710 637 156 150
EcoICRI GAG'CTC
Cuts at: 0 3354 3696 5010
Size: 3354 342 1314
Fragments arranged by size:
3354 1314 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 89 283 380 416 802 1510 1615
Size: 80 9 194 97 36 386 708 105
Cuts at: 1615 2059 2279 2930 3083 3086 3586 3677 3742
Size: 444 220 651 153 3 500 91 65
Cuts at: 3742 3933 4033 4153 4598 5010
Size: 191 100 120 445 412
Fragments arranged by size:
708 651 500 445 444 412 386 220
194 191 153 120 105 100 97 91
80 65 36 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 107 205 1724 5010
Size: 107 98 1519 3286
Fragments arranged by size:
3286 1519 107 98
EcoO109I rG'GnC_Cy
Cuts at: 0 701 2631 4210 4211 4915 4947 5010
Size: 701 1930 1579 1 704 32 63
Fragments arranged by size:
1930 1579 704 701 63 32 1
EcoRI G'AATT_C
Cuts at: 0 1391 5010
Size: 1391 3619
EcoRV GAT'ATC
Cuts at: 0 784 5010
Size: 784 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2056 2424 5010
Size: 2056 368 2586
Fragments arranged by size:
2586 2056 368
HaeII r_GCGC'y
Cuts at: 0 4629 5010
Size: 4629 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 450 482 628 660 768 800 951 983
Size: 450 32 146 32 108 32 151 32
Cuts at: 983 2478 2510 4030 4062 5010
Size: 1495 32 1520 32 948
Fragments arranged by size:
1520 1495 948 450 151 146 108 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3490 3684 5010
Size: 3490 194 1326
Fragments arranged by size:
3490 1326 194
HindIII A'AGCT_T
Cuts at: 0 265 1648 2813 4586 5010
Size: 265 1383 1165 1773 424
Fragments arranged by size:
1773 1383 1165 424 265
HpaI GTT'AAC
Cuts at: 0 3684 5010
Size: 3684 1326
KasI G'GCGC_C
Cuts at: 0 4625 5010
Size: 4625 385
KpnI G_GTAC'C
Cuts at: 0 1666 5010
Size: 1666 3344
MfeI C'AATT_G
Cuts at: 0 3533 5010
Size: 3533 1477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1463 1560 2844 3731 5010
Size: 1463 97 1284 887 1279
Fragments arranged by size:
1463 1284 1279 887 97
MscI TGG'CCA
Cuts at: 0 934 5010
Size: 934 4076
MslI CAynn'nnrTG
Cuts at: 0 22 244 5010
Size: 22 222 4766
Fragments arranged by size:
4766 222 22
MspA1I CmG'CkG
Cuts at: 0 400 843 2827 3796 3836 3927 5010
Size: 400 443 1984 969 40 91 1083
Fragments arranged by size:
1984 1083 969 443 400 91 40
NaeI GCC'GGC
Cuts at: 0 164 5010
Size: 164 4846
NarI GG'CG_CC
Cuts at: 0 4626 5010
Size: 4626 384
NdeI CA'TA_TG
Cuts at: 0 3108 5010
Size: 3108 1902
NgoMIV G'CCGG_C
Cuts at: 0 162 5010
Size: 162 4848
NheI G'CTAG_C
Cuts at: 0 3052 5010
Size: 3052 1958
NspI r_CATG'y
Cuts at: 0 45 366 1704 3813 4642 5010
Size: 45 321 1338 2109 829 368
Fragments arranged by size:
2109 1338 829 368 321 45
PciI A'CATG_T
Cuts at: 0 41 5010
Size: 41 4969
PfoI T'CCnGG_A
Cuts at: 0 2075 2641 4742 5010
Size: 2075 566 2101 268
Fragments arranged by size:
2101 2075 566 268
PmlI CAC'GTG
Cuts at: 0 4152 5010
Size: 4152 858
PpuMI rG'GwC_Cy
Cuts at: 0 701 4947 5010
Size: 701 4246 63
Fragments arranged by size:
4246 701 63
PsiI TTA'TAA
Cuts at: 0 1913 5010
Size: 1913 3097
PspOMI G'GGCC_C
Cuts at: 0 4210 5010
Size: 4210 800
PspXI vC'TCGA_Gb
Cuts at: 0 4063 5010
Size: 4063 947
PstI C_TGCA'G
Cuts at: 0 3835 4985 5010
Size: 3835 1150 25
Fragments arranged by size:
3835 1150 25
PvuII CAG'CTG
Cuts at: 0 400 843 2827 3836 5010
Size: 400 443 1984 1009 1174
Fragments arranged by size:
1984 1174 1009 443 400
RsrII CG'GwC_CG
Cuts at: 0 4125 5010
Size: 4125 885
SacI G_AGCT'C
Cuts at: 0 3356 3698 5010
Size: 3356 342 1312
Fragments arranged by size:
3356 1312 342
SacII CC_GC'GG
Cuts at: 0 3928 5010
Size: 3928 1082
SapI GCTCTTCn'nnn_
Cuts at: 0 3575 5010
Size: 3575 1435
SexAI A'CCwGG_T
Cuts at: 0 2298 5010
Size: 2298 2712
SfcI C'TryA_G
Cuts at: 0 2420 3831 4652 4981 5010
Size: 2420 1411 821 329 29
Fragments arranged by size:
2420 1411 821 329 29
SfoI GGC'GCC
Cuts at: 0 4627 5010
Size: 4627 383
SmlI C'TyrA_G
Cuts at: 0 1477 4063 4088 5010
Size: 1477 2586 25 922
Fragments arranged by size:
2586 1477 922 25
SphI G_CATG'C
Cuts at: 0 366 5010
Size: 366 4644
SspI AAT'ATT
Cuts at: 0 2942 3158 5010
Size: 2942 216 1852
Fragments arranged by size:
2942 1852 216
StuI AGG'CCT
Cuts at: 0 2407 4166 5010
Size: 2407 1759 844
Fragments arranged by size:
2407 1759 844
StyI C'CwwG_G
Cuts at: 0 3365 5010
Size: 3365 1645
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 705 3173 3592 4142 4512 5010
Size: 705 2468 419 550 370 498
Fragments arranged by size:
2468 705 550 498 419 370
TatI w'GTAC_w
Cuts at: 0 4553 5010
Size: 4553 457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1570 1602 4740 4772 5010
Size: 1570 32 3138 32 238
Fragments arranged by size:
3138 1570 238 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1370 5010
Size: 1370 3640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3491 5010
Size: 3491 1519
XhoI C'TCGA_G
Cuts at: 0 4063 5010
Size: 4063 947
XmnI GAAnn'nnTTC
Cuts at: 0 2099 4348 5010
Size: 2099 2249 662
Fragments arranged by size:
2249 2099 662
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlwNI ApaI ApoI
AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NspI
PciI PfoI PmlI PpuMI PsiI PspOMI PspXI PstI
PvuII RsrII SacI SacII SapI SexAI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AsiSI AvrII BaeI BcgI BfrBI
BglI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrGI
BssHII BssSI Bsu36I ClaI CspCI DraIII DrdI EagI
EciI FseI FspI FspAI MluI NcoI NotI NruI
NsiI PacI PasI PflMI PmeI PpiI PshAI PsrI
PvuI SalI SanDI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI XbaI XmaI ZraI