Restriction map for
pF1KSDA1252
(Linear) (Six-base) MAPSORT of: KIAA1252.seq Check: 3283 from: 1 to: 1704
KIAA1252.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 62 941 1704
Size: 62 879 763
Fragments arranged by size:
879 763 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1704
Size: 917 787
AflII C'TTAA_G
Cuts at: 0 388 1704
Size: 388 1316
AflIII A'CryG_T
Cuts at: 0 305 1704
Size: 305 1399
AleI CACnn'nnGTG
Cuts at: 0 1232 1704
Size: 1232 472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 143 1704
Size: 143 1561
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 561 593 1704
Size: 561 32 1111
Fragments arranged by size:
1111 561 32
AlwNI CAG_nnn'CTG
Cuts at: 0 360 1704
Size: 360 1344
BamHI G'GATC_C
Cuts at: 0 1360 1704
Size: 1360 344
BanI G'GyrC_C
Cuts at: 0 1567 1704
Size: 1567 137
BanII G_rGCy'C
Cuts at: 0 41 116 367 476 1704
Size: 41 75 251 109 1228
Fragments arranged by size:
1228 251 109 75 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 171 1558 1623 1704
Size: 171 1387 65 81
Fragments arranged by size:
1387 171 81 65
BciVI GTATCCnnnnn_n'
Cuts at: 0 982 1704
Size: 982 722
BglI GCCn_nnn'nGGC
Cuts at: 0 910 1213 1704
Size: 910 303 491
Fragments arranged by size:
910 491 303
Bme1580I G_kGCm'C
Cuts at: 0 723 1704
Size: 723 981
BmrI ACTGGGnnnn_n'
Cuts at: 0 1436 1704
Size: 1436 268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 417 979 1704
Size: 417 562 725
Fragments arranged by size:
725 562 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 813 1018 1704
Size: 680 133 205 686
Fragments arranged by size:
686 680 205 133
Bpu10I CC'TnA_GC
Cuts at: 0 1406 1704
Size: 1406 298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 1704
Size: 1273 431
BsaHI Gr'CG_yC
Cuts at: 0 410 1704
Size: 410 1294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 1704
Size: 472 1232
BsiHKAI G_wGCw'C
Cuts at: 0 367 476 1004 1704
Size: 367 109 528 700
Fragments arranged by size:
700 528 367 109
Bsp1286I G_dGCh'C
Cuts at: 0 41 116 367 476 723 1004 1704
Size: 41 75 251 109 247 281 700
Fragments arranged by size:
700 281 251 247 109 75 41
BspHI T'CATG_A
Cuts at: 0 1532 1704
Size: 1532 172
BsrDI GCAATG_nn'
Cuts at: 0 96 818 1349 1704
Size: 96 722 531 355
Fragments arranged by size:
722 531 355 96
BsrGI T'GTAC_A
Cuts at: 0 446 1646 1704
Size: 446 1200 58
Fragments arranged by size:
1200 446 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1060 1563 1684 1704
Size: 1060 503 121 20
Fragments arranged by size:
1060 503 121 20
BstYI r'GATC_y
Cuts at: 0 666 885 1360 1704
Size: 666 219 475 344
Fragments arranged by size:
666 475 344 219
BstZ17I GTA'TAC
Cuts at: 0 63 1704
Size: 63 1641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1158 1704
Size: 1158 546
BtsI GCAGTG_nn'
Cuts at: 0 836 1704
Size: 836 868
DraI TTT'AAA
Cuts at: 0 210 1704
Size: 210 1494
EaeI y'GGCC_r
Cuts at: 0 902 1704
Size: 902 802
EarI CTCTTCn'nnn_
Cuts at: 0 813 1704
Size: 813 891
EcoICRI GAG'CTC
Cuts at: 0 365 474 1704
Size: 365 109 1230
Fragments arranged by size:
1230 365 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 813 917 1018 1704
Size: 680 133 104 101 686
Fragments arranged by size:
686 680 133 104 101
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 1704
Size: 481 1223
EcoO109I rG'GnC_Cy
Cuts at: 0 774 1704
Size: 774 930
EcoRV GAT'ATC
Cuts at: 0 528 1704
Size: 528 1176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 678 710 1505 1537 1704
Size: 678 32 795 32 167
Fragments arranged by size:
795 678 167 32 32
HincII GTy'rAC
Cuts at: 0 942 1599 1704
Size: 942 657 105
Fragments arranged by size:
942 657 105
HindIII A'AGCT_T
Cuts at: 0 341 1704
Size: 341 1363
MfeI C'AATT_G
Cuts at: 0 1339 1704
Size: 1339 365
MscI TGG'CCA
Cuts at: 0 904 1704
Size: 904 800
MslI CAynn'nnrTG
Cuts at: 0 643 841 1058 1232 1561 1704
Size: 643 198 217 174 329 143
Fragments arranged by size:
643 329 217 198 174 143
MspA1I CmG'CkG
Cuts at: 0 1045 1704
Size: 1045 659
NspI r_CATG'y
Cuts at: 0 309 1704
Size: 309 1395
PasI CC'CwG_GG
Cuts at: 0 354 1668 1704
Size: 354 1314 36
Fragments arranged by size:
1314 354 36
PciI A'CATG_T
Cuts at: 0 305 1704
Size: 305 1399
PfoI T'CCnGG_A
Cuts at: 0 829 1704
Size: 829 875
PpuMI rG'GwC_Cy
Cuts at: 0 774 1704
Size: 774 930
PshAI GACnn'nnGTC
Cuts at: 0 947 1704
Size: 947 757
PvuII CAG'CTG
Cuts at: 0 1045 1704
Size: 1045 659
SacI G_AGCT'C
Cuts at: 0 367 476 1704
Size: 367 109 1228
Fragments arranged by size:
1228 367 109
SalI G'TCGA_C
Cuts at: 0 940 1704
Size: 940 764
SanDI GG'GwC_CC
Cuts at: 0 774 1704
Size: 774 930
SapI GCTCTTCn'nnn_
Cuts at: 0 813 1704
Size: 813 891
SmlI C'TyrA_G
Cuts at: 0 388 1288 1704
Size: 388 900 416
Fragments arranged by size:
900 416 388
StuI AGG'CCT
Cuts at: 0 31 1704
Size: 31 1673
StyI C'CwwG_G
Cuts at: 0 284 1704
Size: 284 1420
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1019 1066 1455 1555 1681 1704
Size: 1019 47 389 100 126 23
Fragments arranged by size:
1019 389 126 100 47 23
TatI w'GTAC_w
Cuts at: 0 395 446 1109 1646 1704
Size: 395 51 663 537 58
Fragments arranged by size:
663 537 395 58 51
Tth111I GACn'n_nGTC
Cuts at: 0 1660 1704
Size: 1660 44
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 194 1578 1704
Size: 194 1384 126
Fragments arranged by size:
1384 194 126
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AloI AlwNI
BamHI BanI BanII BbsI BciVI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaHI BseRI BsiHKAI Bsp1286I
BspHI BsrDI BsrGI BstXI BstYI BstZ17I BtgZI BtsI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
Hin4I HincII HindIII MfeI MscI MslI MspA1I NspI
PasI PciI PfoI PpuMI PshAI PvuII SacI SalI
SanDI SapI SmlI StuI StyI TaqII TatI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmtI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgI ClaI
CspCI DraIII DrdI EagI EciI EcoRI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmaI XmnI ZraI