Restriction map for
pF1KSDA1244
(Linear) (Six-base) MAPSORT of: KIAA1244.seq Check: 2238 from: 1 to: 5517
KIAA1244.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1287 3975 5517
Size: 1287 2688 1542
Fragments arranged by size:
2688 1542 1287
AatII G_ACGT'C
Cuts at: 0 2804 5517
Size: 2804 2713
AccI GT'mk_AC
Cuts at: 0 656 725 5517
Size: 656 69 4792
Fragments arranged by size:
4792 656 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 994 1243 1697 2055 2437 3247 3691
Size: 388 606 249 454 358 382 810 444
Cuts at: 3691 3876 4375 4387 4942 5161 5208 5517
Size: 185 499 12 555 219 47 309
Fragments arranged by size:
810 606 555 499 454 444 388 382
358 309 249 219 185 47 12
AfeI AGC'GCT
Cuts at: 0 2133 5517
Size: 2133 3384
AflIII A'CryG_T
Cuts at: 0 863 2022 2024 2655 5517
Size: 863 1159 2 631 2862
Fragments arranged by size:
2862 1159 863 631 2
AgeI A'CCGG_T
Cuts at: 0 935 3328 5517
Size: 935 2393 2189
Fragments arranged by size:
2393 2189 935
AhdI GACnn_n'nnGTC
Cuts at: 0 876 5517
Size: 876 4641
AleI CACnn'nnGTG
Cuts at: 0 1879 5517
Size: 1879 3638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1109 5517
Size: 1109 4408
AlwNI CAG_nnn'CTG
Cuts at: 0 1023 1167 1905 2229 3176 3376 5517
Size: 1023 144 738 324 947 200 2141
Fragments arranged by size:
2141 1023 947 738 324 200 144
ApaI G_GGCC'C
Cuts at: 0 3775 5517
Size: 3775 1742
AvaI C'yCGr_G
Cuts at: 0 532 559 849 917 2152 2173 4348 4599
Size: 532 27 290 68 1235 21 2175 251
Cuts at: 4599 4892 5236 5517
Size: 293 344 281
Fragments arranged by size:
2175 1235 532 344 293 290 281 251
68 27 21
AvrII C'CTAG_G
Cuts at: 0 927 5517
Size: 927 4590
BamHI G'GATC_C
Cuts at: 0 4977 5517
Size: 4977 540
BanI G'GyrC_C
Cuts at: 0 289 1266 2050 2080 4390 5517
Size: 289 977 784 30 2310 1127
Fragments arranged by size:
2310 1127 977 784 289 30
BanII G_rGCy'C
Cuts at: 0 103 305 1352 1648 2167 3132 3775 5287
Size: 103 202 1047 296 519 965 643 1512
Cuts at: 5287 5517
Size: 230
Fragments arranged by size:
1512 1047 965 643 519 296 230 202
103
BbeI G_GCGC'C
Cuts at: 0 1270 4394 5517
Size: 1270 3124 1123
Fragments arranged by size:
3124 1270 1123
BbsI GAAGACnn'nnnn_
Cuts at: 0 1317 3264 4571 5517
Size: 1317 1947 1307 946
Fragments arranged by size:
1947 1317 1307 946
BbvCI CC'TCA_GC
Cuts at: 0 1153 1791 1975 2554 5517
Size: 1153 638 184 579 2963
Fragments arranged by size:
2963 1153 638 579 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1710 1744 4215 4249 5517
Size: 1710 34 2471 34 1268
Fragments arranged by size:
2471 1710 1268 34 34
BclI T'GATC_A
Cuts at: 0 819 3656 5517
Size: 819 2837 1861
Fragments arranged by size:
2837 1861 819
BfrBI ATG'CAT
Cuts at: 0 230 5517
Size: 230 5287
BglI GCCn_nnn'nGGC
Cuts at: 0 1807 3703 4249 5517
Size: 1807 1896 546 1268
Fragments arranged by size:
1896 1807 1268 546
BlpI GC'TnA_GC
Cuts at: 0 304 628 1756 4645 5517
Size: 304 324 1128 2889 872
Fragments arranged by size:
2889 1128 872 324 304
Bme1580I G_kGCm'C
Cuts at: 0 294 2053 2892 3117 3775 3816 4632 5517
Size: 294 1759 839 225 658 41 816 885
Fragments arranged by size:
1759 885 839 816 658 294 225 41
BmgBI CAC'GTC
Cuts at: 0 864 1953 5517
Size: 864 1089 3564
Fragments arranged by size:
3564 1089 864
BmrI ACTGGGnnnn_n'
Cuts at: 0 1856 3088 3558 3999 5155 5517
Size: 1856 1232 470 441 1156 362
Fragments arranged by size:
1856 1232 1156 470 441 362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 1781 5517
Size: 55 1726 3736
Fragments arranged by size:
3736 1726 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1927 2008 2077 2625 2957 3379 3711 4144
Size: 1927 81 69 548 332 422 332 433
Cuts at: 4144 4232 4771 5383 5517
Size: 88 539 612 134
Fragments arranged by size:
1927 612 548 539 433 422 332 332
134 88 81 69
Bpu10I CC'TnA_GC
Cuts at: 0 1110 1153 1791 1975 2203 2554 4915 5268
Size: 1110 43 638 184 228 351 2361 353
Cuts at: 5268 5517
Size: 249
Fragments arranged by size:
2361 1110 638 353 351 249 228 184
43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 379 577 2489 3826 5517
Size: 342 37 198 1912 1337 1691
Fragments arranged by size:
1912 1691 1337 342 198 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 2868 5517
Size: 2868 2649
BsaAI yAC'GTr
Cuts at: 0 2025 2046 2592 5442 5517
Size: 2025 21 546 2850 75
Fragments arranged by size:
2850 2025 546 75 21
BsaHI Gr'CG_yC
Cuts at: 0 1267 1712 2801 4391 5517
Size: 1267 445 1089 1590 1126
Fragments arranged by size:
1590 1267 1126 1089 445
BsaWI w'CCGG_w
Cuts at: 0 935 3328 5517
Size: 935 2393 2189
Fragments arranged by size:
2393 2189 935
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1494 1524 5517
Size: 1494 30 3993
Fragments arranged by size:
3993 1494 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 376 495 1024 1081 1204 1360 3484
Size: 184 192 119 529 57 123 156 2124
Cuts at: 3484 3802 3924 4780 5517
Size: 318 122 856 737
Fragments arranged by size:
2124 856 737 529 318 192 184 156
123 122 119 57
BseYI C'CCAG_C
Cuts at: 0 53 130 285 3117 3861 3980 4541 4954
Size: 53 77 155 2832 744 119 561 413
Cuts at: 4954 4981 5186 5481 5517
Size: 27 205 295 36
Fragments arranged by size:
2832 744 561 413 295 205 155 119
77 53 36 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 694 1181 1321 1924 1945 4681 5517
Size: 261 433 487 140 603 21 2736 836
Fragments arranged by size:
2736 836 603 487 433 261 140 21
BsiEI CG_ry'CG
Cuts at: 0 1258 4487 5517
Size: 1258 3229 1030
Fragments arranged by size:
3229 1258 1030
BsiHKAI G_wGCw'C
Cuts at: 0 14 305 1352 1367 1648 2063 2180 2209
Size: 14 291 1047 15 281 415 117 29
Cuts at: 2209 2330 3614 4012 4901 5287 5517
Size: 121 1284 398 889 386 230
Fragments arranged by size:
1284 1047 889 415 398 386 291 281
230 121 117 29 15 14
BsmI GAATG_Cn'
Cuts at: 0 2550 5013 5217 5517
Size: 2550 2463 204 300
Fragments arranged by size:
2550 2463 300 204
BsmBI CGTCTCn'nnnn_
Cuts at: 0 115 616 5300 5517
Size: 115 501 4684 217
Fragments arranged by size:
4684 501 217 115
Bsp1286I G_dGCh'C
Cuts at: 0 14 103 294 305 1352 1367 1648 2053
Size: 14 89 191 11 1047 15 281 405
Cuts at: 2053 2063 2167 2180 2209 2330 2892 3117 3132
Size: 10 104 13 29 121 562 225 15
Cuts at: 3132 3614 3775 3816 4012 4632 4901 5287 5517
Size: 482 161 41 196 620 269 386 230
Fragments arranged by size:
1047 620 562 482 405 386 281 269
230 225 196 191 161 121 104 89
41 29 15 15 14 13 11 10
BspHI T'CATG_A
Cuts at: 0 1289 2297 5517
Size: 1289 1008 3220
Fragments arranged by size:
3220 1289 1008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 1287 3866 3975 4310 4949 5517
Size: 845 442 2579 109 335 639 568
Fragments arranged by size:
2579 845 639 568 442 335 109
BsrBI CCG'CTC
Cuts at: 0 1081 1707 2303 5267 5517
Size: 1081 626 596 2964 250
Fragments arranged by size:
2964 1081 626 596 250
BsrDI GCAATG_nn'
Cuts at: 0 2486 5517
Size: 2486 3031
BsrFI r'CCGG_y
Cuts at: 0 935 3244 3328 4927 5517
Size: 935 2309 84 1599 590
Fragments arranged by size:
2309 1599 935 590 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 5517
Size: 345 5172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1656 5517
Size: 1656 3861
BstYI r'GATC_y
Cuts at: 0 189 748 2858 4977 5321 5517
Size: 189 559 2110 2119 344 196
Fragments arranged by size:
2119 2110 559 344 196 189
Bsu36I CC'TnA_GG
Cuts at: 0 3169 5517
Size: 3169 2348
BtgI C'CryG_G
Cuts at: 0 398 460 1233 2283 2954 3391 3974 5030
Size: 398 62 773 1050 671 437 583 1056
Cuts at: 5030 5517
Size: 487
Fragments arranged by size:
1056 1050 773 671 583 487 437 398
62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2090 2411 4432 5517
Size: 2090 321 2021 1085
Fragments arranged by size:
2090 2021 1085 321
BtsI GCAGTG_nn'
Cuts at: 0 1200 1473 1943 3288 3488 4552 5414 5517
Size: 1200 273 470 1345 200 1064 862 103
Fragments arranged by size:
1345 1200 1064 862 470 273 200 103
ClaI AT'CG_AT
Cuts at: 0 1604 5517
Size: 1604 3913
DraIII CAC_nnn'GTG
Cuts at: 0 33 63 461 2628 4007 5517
Size: 33 30 398 2167 1379 1510
Fragments arranged by size:
2167 1510 1379 398 33 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 2815 5517
Size: 2815 2702
EaeI y'GGCC_r
Cuts at: 0 1255 1479 2017 2315 3191 3704 4484 5517
Size: 1255 224 538 298 876 513 780 1033
Fragments arranged by size:
1255 1033 876 780 538 513 298 224
EagI C'GGCC_G
Cuts at: 0 1255 4484 5517
Size: 1255 3229 1033
Fragments arranged by size:
3229 1255 1033
EarI CTCTTCn'nnn_
Cuts at: 0 899 1835 2758 4181 4609 5517
Size: 899 936 923 1423 428 908
Fragments arranged by size:
1423 936 923 908 899 428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1003 2524 3511 5517
Size: 1003 1521 987 2006
Fragments arranged by size:
2006 1521 1003 987
EcoICRI GAG'CTC
Cuts at: 0 303 1350 1646 5285 5517
Size: 303 1047 296 3639 232
Fragments arranged by size:
3639 1047 303 296 232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 994 1243 1697 1927 2008 2055 2077
Size: 388 606 249 454 230 81 47 22
Cuts at: 2077 2437 2625 2957 3247 3379 3691 3711 3876
Size: 360 188 332 290 132 312 20 165
Cuts at: 3876 4144 4232 4375 4387 4771 4942 5161 5208
Size: 268 88 143 12 384 171 219 47
Cuts at: 5208 5383 5517
Size: 175 134
Fragments arranged by size:
606 454 388 384 360 332 312 290
268 249 230 219 188 175 171 165
143 134 132 88 81 47 47 22
20 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2629 4510 5517
Size: 2629 1881 1007
Fragments arranged by size:
2629 1881 1007
EcoO109I rG'GnC_Cy
Cuts at: 0 127 740 2148 2241 3675 3772 3855 4201
Size: 127 613 1408 93 1434 97 83 346
Cuts at: 4201 4503 4938 5149 5233 5261 5517
Size: 302 435 211 84 28 256
Fragments arranged by size:
1434 1408 613 435 346 302 256 211
127 97 93 84 83 28
EcoRV GAT'ATC
Cuts at: 0 3639 5517
Size: 3639 1878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 3696 4130 5517
Size: 109 3587 434 1387
Fragments arranged by size:
3587 1387 434 109
HaeII r_GCGC'y
Cuts at: 0 1004 1270 2135 2199 2522 3177 4394 5517
Size: 1004 266 865 64 323 655 1217 1123
Fragments arranged by size:
1217 1123 1004 865 655 323 266 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 811 843 862 894 1110 1142
Size: 315 32 464 32 19 32 216 32
Cuts at: 1142 1597 1629 2068 2100 3544 3576 3814 3846
Size: 455 32 439 32 1444 32 238 32
Cuts at: 3846 5517
Size: 1671
Fragments arranged by size:
1671 1444 464 455 439 315 238 216
32 32 32 32 32 32 32 32
19
HindIII A'AGCT_T
Cuts at: 0 248 2993 3621 5517
Size: 248 2745 628 1896
Fragments arranged by size:
2745 1896 628 248
KasI G'GCGC_C
Cuts at: 0 1266 4390 5517
Size: 1266 3124 1127
Fragments arranged by size:
3124 1266 1127
MfeI C'AATT_G
Cuts at: 0 3384 5517
Size: 3384 2133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 884 2300 2781 5517
Size: 860 24 1416 481 2736
Fragments arranged by size:
2736 1416 860 481 24
MscI TGG'CCA
Cuts at: 0 1481 2019 3193 3706 5517
Size: 1481 538 1174 513 1811
Fragments arranged by size:
1811 1481 1174 538 513
MslI CAynn'nnrTG
Cuts at: 0 1435 1720 1879 2741 4273 4735 5512 5517
Size: 1435 285 159 862 1532 462 777 5
Fragments arranged by size:
1532 1435 862 777 462 285 159 5
MspA1I CmG'CkG
Cuts at: 0 425 432 1195 1310 1485 1813 2285 2415
Size: 425 7 763 115 175 328 472 130
Cuts at: 2415 2781 3366 3373 3769 3878 3896 5172 5430
Size: 366 585 7 396 109 18 1276 258
Cuts at: 5430 5517
Size: 87
Fragments arranged by size:
1276 763 585 472 425 396 366 328
258 175 130 115 109 87 18 7
7
NaeI GCC'GGC
Cuts at: 0 3246 5517
Size: 3246 2271
NarI GG'CG_CC
Cuts at: 0 1267 4391 5517
Size: 1267 3124 1126
Fragments arranged by size:
3124 1267 1126
NcoI C'CATG_G
Cuts at: 0 398 2954 3974 5030 5517
Size: 398 2556 1020 1056 487
Fragments arranged by size:
2556 1056 1020 487 398
NgoMIV G'CCGG_C
Cuts at: 0 3244 5517
Size: 3244 2273
NsiI A_TGCA'T
Cuts at: 0 232 5517
Size: 232 5285
NspI r_CATG'y
Cuts at: 0 230 1380 2659 5517
Size: 230 1150 1279 2858
Fragments arranged by size:
2858 1279 1150 230
PasI CC'CwG_GG
Cuts at: 0 5153 5517
Size: 5153 364
PciI A'CATG_T
Cuts at: 0 2655 5517
Size: 2655 2862
PflMI CCAn_nnn'nTGG
Cuts at: 0 1488 1627 3469 4446 5517
Size: 1488 139 1842 977 1071
Fragments arranged by size:
1842 1488 1071 977 139
PfoI T'CCnGG_A
Cuts at: 0 2237 2249 4209 5517
Size: 2237 12 1960 1308
Fragments arranged by size:
2237 1960 1308 12
PmlI CAC'GTG
Cuts at: 0 2025 2592 5442 5517
Size: 2025 567 2850 75
Fragments arranged by size:
2850 2025 567 75
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 5517
Size: 1081 32 4404
Fragments arranged by size:
4404 1081 32
PpuMI rG'GwC_Cy
Cuts at: 0 127 740 2148 2241 3675 3855 4503 4938
Size: 127 613 1408 93 1434 180 648 435
Cuts at: 4938 5149 5517
Size: 211 368
Fragments arranged by size:
1434 1408 648 613 435 368 211 180
127 93
PshAI GACnn'nnGTC
Cuts at: 0 2658 2701 3329 5517
Size: 2658 43 628 2188
Fragments arranged by size:
2658 2188 628 43
PspOMI G'GGCC_C
Cuts at: 0 3771 5517
Size: 3771 1746
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5008 5040 5517
Size: 5008 32 477
Fragments arranged by size:
5008 477 32
PstI C_TGCA'G
Cuts at: 0 733 1088 5517
Size: 733 355 4429
Fragments arranged by size:
4429 733 355
PvuII CAG'CTG
Cuts at: 0 1195 1485 1813 2415 2781 3366 5430 5517
Size: 1195 290 328 602 366 585 2064 87
Fragments arranged by size:
2064 1195 602 585 366 328 290 87
SacI G_AGCT'C
Cuts at: 0 305 1352 1648 5287 5517
Size: 305 1047 296 3639 230
Fragments arranged by size:
3639 1047 305 296 230
SacII CC_GC'GG
Cuts at: 0 2286 5517
Size: 2286 3231
SanDI GG'GwC_CC
Cuts at: 0 2148 5149 5517
Size: 2148 3001 368
Fragments arranged by size:
3001 2148 368
SapI GCTCTTCn'nnn_
Cuts at: 0 899 5517
Size: 899 4618
ScaI AGT'ACT
Cuts at: 0 826 3958 5517
Size: 826 3132 1559
Fragments arranged by size:
3132 1559 826
SexAI A'CCwGG_T
Cuts at: 0 2624 2810 2936 5517
Size: 2624 186 126 2581
Fragments arranged by size:
2624 2581 186 126
SfcI C'TryA_G
Cuts at: 0 729 1084 1245 1502 5517
Size: 729 355 161 257 4015
Fragments arranged by size:
4015 729 355 257 161
SfoI GGC'GCC
Cuts at: 0 1268 4392 5517
Size: 1268 3124 1125
Fragments arranged by size:
3124 1268 1125
SmaI CCC'GGG
Cuts at: 0 919 4350 4894 5517
Size: 919 3431 544 623
Fragments arranged by size:
3431 919 623 544
SmlI C'TyrA_G
Cuts at: 0 321 358 532 592 2504 3841 5517
Size: 321 37 174 60 1912 1337 1676
Fragments arranged by size:
1912 1676 1337 321 174 60 37
SphI G_CATG'C
Cuts at: 0 230 5517
Size: 230 5287
SspI AAT'ATT
Cuts at: 0 3224 5517
Size: 3224 2293
StuI AGG'CCT
Cuts at: 0 1031 1336 1985 5517
Size: 1031 305 649 3532
Fragments arranged by size:
3532 1031 649 305
StyI C'CwwG_G
Cuts at: 0 398 927 2142 2318 2393 2954 3974 5030
Size: 398 529 1215 176 75 561 1020 1056
Cuts at: 5030 5202 5517
Size: 172 315
Fragments arranged by size:
1215 1056 1020 561 529 398 315 176
172 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 277 1459 2525 4018 4665 5080 5461
Size: 235 42 1182 1066 1493 647 415 381
Cuts at: 5461 5517
Size: 56
Fragments arranged by size:
1493 1182 1066 647 415 381 235 56
42
TatI w'GTAC_w
Cuts at: 0 824 3956 5517
Size: 824 3132 1561
Fragments arranged by size:
3132 1561 824
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2140 2172 3991 4023 4030 4062 5083 5115
Size: 2140 32 1819 32 7 32 1021 32
Cuts at: 5115 5517
Size: 402
Fragments arranged by size:
2140 1819 1021 402 32 32 32 32
7
Tth111I GACn'n_nGTC
Cuts at: 0 738 2245 5517
Size: 738 1507 3272
Fragments arranged by size:
3272 1507 738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2074 2122 2832 3729 4962 5517
Size: 2074 48 710 897 1233 555
Fragments arranged by size:
2074 1233 897 710 555 48
XhoI C'TCGA_G
Cuts at: 0 532 5517
Size: 532 4985
XmaI C'CCGG_G
Cuts at: 0 917 4348 4892 5517
Size: 917 3431 544 625
Fragments arranged by size:
3431 917 625 544
XmnI GAAnn'nnTTC
Cuts at: 0 1285 2996 5517
Size: 1285 1711 2521
Fragments arranged by size:
2521 1711 1285
ZraI GAC'GTC
Cuts at: 0 2802 5517
Size: 2802 2715
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuII SacI SacII SanDI SapI ScaI SexAI SfcI
SfoI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AloI ApaLI ApoI AscI AseI
AsiSI BaeI BciVI BglII BmtI BsaBI BsiWI BspEI
BsrGI BssHII BssSI BstBI BstEII BstZ17I CspCI DraI
EcoRI FseI FspI FspAI HincII HpaI KpnI MluI
NdeI NheI NotI NruI PacI PmeI PsiI PspXI
PvuI RsrII SalI SbfI SfiI SgfI SgrAI SnaBI
SpeI SrfI SwaI XbaI