Restriction map for
pF1KA1230
(Linear) (Six-base) MAPSORT of: KIAA1230.seq Check: 1823 from: 1 to: 3033
KIAA1230.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 2364 3033
Size: 2364 669
AclI AA'CG_TT
Cuts at: 0 3029 3033
Size: 3029 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 337 649 805 854 1429 1439 2150
Size: 241 96 312 156 49 575 10 711
Cuts at: 2150 2302 2948 3033
Size: 152 646 85
Fragments arranged by size:
711 646 575 312 241 156 152 96
85 49 10
AflIII A'CryG_T
Cuts at: 0 20 1823 2951 2969 3033
Size: 20 1803 1128 18 64
Fragments arranged by size:
1803 1128 64 20 18
AgeI A'CCGG_T
Cuts at: 0 2870 3033
Size: 2870 163
AhdI GACnn_n'nnGTC
Cuts at: 0 2274 3033
Size: 2274 759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2030 3033
Size: 2030 1003
AlwNI CAG_nnn'CTG
Cuts at: 0 996 1246 2334 2689 3033
Size: 996 250 1088 355 344
Fragments arranged by size:
1088 996 355 344 250
ApaI G_GGCC'C
Cuts at: 0 1874 3033
Size: 1874 1159
ApaLI G'TGCA_C
Cuts at: 0 1619 3033
Size: 1619 1414
ApoI r'AATT_y
Cuts at: 0 1837 1995 2733 3033
Size: 1837 158 738 300
Fragments arranged by size:
1837 738 300 158
AvaI C'yCGr_G
Cuts at: 0 1889 3033
Size: 1889 1144
BanI G'GyrC_C
Cuts at: 0 1333 3033
Size: 1333 1700
BanII G_rGCy'C
Cuts at: 0 144 1470 1874 1987 2510 3033
Size: 144 1326 404 113 523 523
Fragments arranged by size:
1326 523 523 404 144 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 1456 2595 3026 3033
Size: 164 1292 1139 431 7
Fragments arranged by size:
1292 1139 431 164 7
BbvCI CC'TCA_GC
Cuts at: 0 2627 3033
Size: 2627 406
BglI GCCn_nnn'nGGC
Cuts at: 0 1515 3033
Size: 1515 1518
BglII A'GATC_T
Cuts at: 0 981 1564 1665 2052 3033
Size: 981 583 101 387 981
Fragments arranged by size:
981 981 583 387 101
Bme1580I G_kGCm'C
Cuts at: 0 1623 1874 3033
Size: 1623 251 1159
Fragments arranged by size:
1623 1159 251
BmrI ACTGGGnnnn_n'
Cuts at: 0 1154 2175 2208 2993 3033
Size: 1154 1021 33 785 40
Fragments arranged by size:
1154 1021 785 40 33
BmtI G_CTAG'C
Cuts at: 0 2110 3033
Size: 2110 923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1141 1721 2086 3033
Size: 1127 14 580 365 947
Fragments arranged by size:
1127 947 580 365 14
Bpu10I CC'TnA_GC
Cuts at: 0 386 1286 2627 3033
Size: 386 900 1341 406
Fragments arranged by size:
1341 900 406 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 3033
Size: 228 2805
BsaI GGTCTCn'nnnn_
Cuts at: 0 1892 3033
Size: 1892 1141
BsaBI GATnn'nnATC
Cuts at: 0 302 3033
Size: 302 2731
BsaWI w'CCGG_w
Cuts at: 0 2870 3033
Size: 2870 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 869 899 1485 1515 3033
Size: 869 30 586 30 1518
Fragments arranged by size:
1518 869 586 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1077 1862 3033
Size: 1077 785 1171
Fragments arranged by size:
1171 1077 785
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 3033
Size: 130 2903
BsiHKAI G_wGCw'C
Cuts at: 0 144 431 1470 1623 1661 3033
Size: 144 287 1039 153 38 1372
Fragments arranged by size:
1372 1039 287 153 144 38
BsmI GAATG_Cn'
Cuts at: 0 450 2079 2249 2920 3033
Size: 450 1629 170 671 113
Fragments arranged by size:
1629 671 450 170 113
Bsp1286I G_dGCh'C
Cuts at: 0 144 431 1470 1623 1661 1874 1987 2510
Size: 144 287 1039 153 38 213 113 523
Cuts at: 2510 3033
Size: 523
Fragments arranged by size:
1039 523 523 287 213 153 144 113
38
BspHI T'CATG_A
Cuts at: 0 1766 3033
Size: 1766 1267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 170 1941 3033
Size: 52 118 1771 1092
Fragments arranged by size:
1771 1092 118 52
BsrFI r'CCGG_y
Cuts at: 0 2870 3033
Size: 2870 163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2470 3033
Size: 2470 563
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1398 3033
Size: 1398 1635
BstYI r'GATC_y
Cuts at: 0 218 510 981 1153 1564 1665 2052 2125
Size: 218 292 471 172 411 101 387 73
Cuts at: 2125 3033
Size: 908
Fragments arranged by size:
908 471 411 387 292 218 172 101
73
Bsu36I CC'TnA_GG
Cuts at: 0 2335 3033
Size: 2335 698
BtgI C'CryG_G
Cuts at: 0 1529 1865 2453 2499 2654 3033
Size: 1529 336 588 46 155 379
Fragments arranged by size:
1529 588 379 336 155 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2379 3033
Size: 2379 654
BtsI GCAGTG_nn'
Cuts at: 0 2496 3033
Size: 2496 537
DraI TTT'AAA
Cuts at: 0 806 1590 2826 2976 3033
Size: 806 784 1236 150 57
Fragments arranged by size:
1236 806 784 150 57
DraIII CAC_nnn'GTG
Cuts at: 0 153 3033
Size: 153 2880
EaeI y'GGCC_r
Cuts at: 0 704 1575 1687 2022 2374 2597 3033
Size: 704 871 112 335 352 223 436
Fragments arranged by size:
871 704 436 352 335 223 112
EarI CTCTTCn'nnn_
Cuts at: 0 419 1055 1187 3033
Size: 419 636 132 1846
Fragments arranged by size:
1846 636 419 132
EcoICRI GAG'CTC
Cuts at: 0 142 1468 3033
Size: 142 1326 1565
Fragments arranged by size:
1565 1326 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 337 649 805 854 1127 1141 1429
Size: 241 96 312 156 49 273 14 288
Cuts at: 1429 1439 1721 2086 2150 2302 2948 3033
Size: 10 282 365 64 152 646 85
Fragments arranged by size:
646 365 312 288 282 273 241 156
152 96 85 64 49 14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2851 3033
Size: 2851 182
EcoO109I rG'GnC_Cy
Cuts at: 0 1493 1508 1870 1871 2331 3033
Size: 1493 15 362 1 460 702
Fragments arranged by size:
1493 702 460 362 15 1
EcoRI G'AATT_C
Cuts at: 0 1837 1995 3033
Size: 1837 158 1038
Fragments arranged by size:
1837 1038 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 203 235 325 357 1485 1517
Size: 91 32 80 32 90 32 1128 32
Cuts at: 1517 1695 1713 1727 1745 1810 1842 2260 2292
Size: 178 18 14 18 65 32 418 32
Cuts at: 2292 2412 2444 3007 3033
Size: 120 32 563 26
Fragments arranged by size:
1128 563 418 178 120 91 90 80
65 32 32 32 32 32 32 32
26 18 18 14
HincII GTy'rAC
Cuts at: 0 368 1280 3033
Size: 368 912 1753
Fragments arranged by size:
1753 912 368
HindIII A'AGCT_T
Cuts at: 0 679 3033
Size: 679 2354
HpaI GTT'AAC
Cuts at: 0 368 3033
Size: 368 2665
MfeI C'AATT_G
Cuts at: 0 1959 3033
Size: 1959 1074
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 488 2162 3033
Size: 17 471 1674 871
Fragments arranged by size:
1674 871 471 17
MscI TGG'CCA
Cuts at: 0 706 2024 2599 3033
Size: 706 1318 575 434
Fragments arranged by size:
1318 706 575 434
MslI CAynn'nnrTG
Cuts at: 0 1528 3033
Size: 1528 1505
MspA1I CmG'CkG
Cuts at: 0 553 759 1867 3033
Size: 553 206 1108 1166
Fragments arranged by size:
1166 1108 553 206
NcoI C'CATG_G
Cuts at: 0 1529 2499 2654 3033
Size: 1529 970 155 379
Fragments arranged by size:
1529 970 379 155
NheI G'CTAG_C
Cuts at: 0 2106 3033
Size: 2106 927
NspI r_CATG'y
Cuts at: 0 24 1827 2955 2973 3033
Size: 24 1803 1128 18 60
Fragments arranged by size:
1803 1128 60 24 18
PasI CC'CwG_GG
Cuts at: 0 1513 2121 3033
Size: 1513 608 912
Fragments arranged by size:
1513 912 608
PciI A'CATG_T
Cuts at: 0 20 1823 2951 2969 3033
Size: 20 1803 1128 18 64
Fragments arranged by size:
1803 1128 64 20 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 2121 3033
Size: 2121 912
PpuMI rG'GwC_Cy
Cuts at: 0 1493 2331 3033
Size: 1493 838 702
Fragments arranged by size:
1493 838 702
PspOMI G'GGCC_C
Cuts at: 0 1870 3033
Size: 1870 1163
PstI C_TGCA'G
Cuts at: 0 33 467 1385 3033
Size: 33 434 918 1648
Fragments arranged by size:
1648 918 434 33
PvuII CAG'CTG
Cuts at: 0 553 3033
Size: 553 2480
SacI G_AGCT'C
Cuts at: 0 144 1470 3033
Size: 144 1326 1563
Fragments arranged by size:
1563 1326 144
SacII CC_GC'GG
Cuts at: 0 1868 3033
Size: 1868 1165
SanDI GG'GwC_CC
Cuts at: 0 1493 3033
Size: 1493 1540
SapI GCTCTTCn'nnn_
Cuts at: 0 419 1055 3033
Size: 419 636 1978
Fragments arranged by size:
1978 636 419
SexAI A'CCwGG_T
Cuts at: 0 374 2829 3033
Size: 374 2455 204
Fragments arranged by size:
2455 374 204
SfcI C'TryA_G
Cuts at: 0 29 463 554 1381 3033
Size: 29 434 91 827 1652
Fragments arranged by size:
1652 827 434 91 29
SmlI C'TyrA_G
Cuts at: 0 207 1889 3033
Size: 207 1682 1144
Fragments arranged by size:
1682 1144 207
SspI AAT'ATT
Cuts at: 0 82 3033
Size: 82 2951
StyI C'CwwG_G
Cuts at: 0 1038 1329 1529 1943 2499 2654 3033
Size: 1038 291 200 414 556 155 379
Fragments arranged by size:
1038 556 414 379 291 200 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1152 2583 3033
Size: 1152 1431 450
Fragments arranged by size:
1431 1152 450
TatI w'GTAC_w
Cuts at: 0 912 2673 3033
Size: 912 1761 360
Fragments arranged by size:
1761 912 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 3033
Size: 1170 32 1831
Fragments arranged by size:
1831 1170 32
XbaI T'CTAG_A
Cuts at: 0 2055 2517 3033
Size: 2055 462 516
Fragments arranged by size:
2055 516 462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 699 1045 1146 1934 3033
Size: 699 346 101 788 1099
Fragments arranged by size:
1099 788 699 346 101
XhoI C'TCGA_G
Cuts at: 0 1889 3033
Size: 1889 1144
XmnI GAAnn'nnTTC
Cuts at: 0 254 983 1442 3033
Size: 254 729 459 1591
Fragments arranged by size:
1591 729 459 254
Enzymes that do cut:
AccI AclI AcuI AflIII AgeI AhdI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BglI BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NheI NspI
PasI PciI PflMI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SapI SexAI SfcI SmlI SspI StyI
TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AleI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BlpI BmgBI BplI BsaAI BsaHI BseYI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I ClaI CspCI DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HaeII KasI
KpnI MluI NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XmaI ZraI