Restriction map for
pF1KSDA1205
(Linear) (Six-base) MAPSORT of: KIAA1205.seq Check: 1024 from: 1 to: 3645
KIAA1205.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 782 3645
Size: 782 2863
Acc65I G'GTAC_C
Cuts at: 0 2459 3645
Size: 2459 1186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1666 2962 3247 3585 3645
Size: 1222 444 1296 285 338 60
Fragments arranged by size:
1296 1222 444 338 285 60
AflII C'TTAA_G
Cuts at: 0 1210 3645
Size: 1210 2435
AflIII A'CryG_T
Cuts at: 0 2779 3269 3645
Size: 2779 490 376
Fragments arranged by size:
2779 490 376
AhdI GACnn_n'nnGTC
Cuts at: 0 3003 3645
Size: 3003 642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2429 3645
Size: 2429 1216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 379 411 820 852 3645
Size: 379 32 409 32 2793
Fragments arranged by size:
2793 409 379 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1282 2095 3046 3144 3504 3638 3645
Size: 1282 813 951 98 360 134 7
Fragments arranged by size:
1282 951 813 360 134 98 7
ApaI G_GGCC'C
Cuts at: 0 512 545 1097 2980 3645
Size: 512 33 552 1883 665
Fragments arranged by size:
1883 665 552 512 33
ApoI r'AATT_y
Cuts at: 0 2374 3645
Size: 2374 1271
AvaI C'yCGr_G
Cuts at: 0 85 145 187 544 1136 1302 1313 1493
Size: 85 60 42 357 592 166 11 180
Cuts at: 1493 1657 1879 2149 2722 3645
Size: 164 222 270 573 923
Fragments arranged by size:
923 592 573 357 270 222 180 166
164 85 60 42 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 3645
Size: 215 33 3397
Fragments arranged by size:
3397 215 33
BamHI G'GATC_C
Cuts at: 0 159 258 1061 3645
Size: 159 99 803 2584
Fragments arranged by size:
2584 803 159 99
BanI G'GyrC_C
Cuts at: 0 18 169 213 268 295 303 648 782
Size: 18 151 44 55 27 8 345 134
Cuts at: 782 995 1123 1183 1570 2459 3520 3645
Size: 213 128 60 387 889 1061 125
Fragments arranged by size:
1061 889 387 345 213 151 134 128
125 60 55 44 27 18 8
BanII G_rGCy'C
Cuts at: 0 119 195 512 545 1097 1199 1853 1886
Size: 119 76 317 33 552 102 654 33
Cuts at: 1886 2126 2714 2980 3021 3310 3402 3645
Size: 240 588 266 41 289 92 243
Fragments arranged by size:
654 588 552 317 289 266 243 240
119 102 92 76 41 33 33
BbeI G_GCGC'C
Cuts at: 0 173 217 272 307 652 786 999 1127
Size: 173 44 55 35 345 134 213 128
Cuts at: 1127 1574 3524 3645
Size: 447 1950 121
Fragments arranged by size:
1950 447 345 213 173 134 128 121
55 44 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1697 2150 3645
Size: 1697 453 1495
Fragments arranged by size:
1697 1495 453
BbvCI CC'TCA_GC
Cuts at: 0 1169 1937 1943 2288 3645
Size: 1169 768 6 345 1357
Fragments arranged by size:
1357 1169 768 345 6
BciVI GTATCCnnnnn_n'
Cuts at: 0 216 3427 3645
Size: 216 3211 218
Fragments arranged by size:
3211 218 216
BglI GCCn_nnn'nGGC
Cuts at: 0 293 1041 1579 1816 2104 2984 3645
Size: 293 748 538 237 288 880 661
Fragments arranged by size:
880 748 661 538 293 288 237
BlpI GC'TnA_GC
Cuts at: 0 1456 3241 3370 3645
Size: 1456 1785 129 275
Fragments arranged by size:
1785 1456 275 129
Bme1580I G_kGCm'C
Cuts at: 0 512 545 1097 1186 1232 2980 3645
Size: 512 33 552 89 46 1748 665
Fragments arranged by size:
1748 665 552 512 89 46 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 955 1850 2542 3645
Size: 955 895 692 1103
Fragments arranged by size:
1103 955 895 692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1328 1710 1746 2748 3645
Size: 1328 382 36 1002 897
Fragments arranged by size:
1328 1002 897 382 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 217 897 1093 1597 1915 3035 3166
Size: 133 84 680 196 504 318 1120 131
Cuts at: 3166 3645
Size: 479
Fragments arranged by size:
1120 680 504 479 318 196 133 131
84
Bpu10I CC'TnA_GC
Cuts at: 0 1169 1937 1943 2288 2708 3645
Size: 1169 768 6 345 420 937
Fragments arranged by size:
1169 937 768 420 345 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1387 2290 3364 3645
Size: 739 648 903 1074 281
Fragments arranged by size:
1074 903 739 648 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 915 1706 2641 3645
Size: 915 791 935 1004
Fragments arranged by size:
1004 935 915 791
BsaAI yAC'GTr
Cuts at: 0 2511 2782 3096 3645
Size: 2511 271 314 549
Fragments arranged by size:
2511 549 314 271
BsaHI Gr'CG_yC
Cuts at: 0 170 214 269 304 529 649 783 996
Size: 170 44 55 35 225 120 134 213
Cuts at: 996 1124 1447 1571 3051 3521 3645
Size: 128 323 124 1480 470 124
Fragments arranged by size:
1480 470 323 225 213 170 134 128
124 124 120 55 44 35
BsaWI w'CCGG_w
Cuts at: 0 1549 2382 3086 3312 3645
Size: 1549 833 704 226 333
Fragments arranged by size:
1549 833 704 333 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 379 409 820 850 895 925 1354 1384
Size: 379 30 411 30 45 30 429 30
Cuts at: 1384 1905 1935 3645
Size: 521 30 1710
Fragments arranged by size:
1710 521 429 411 379 45 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 376 403 1186 1363 1654 1668 1722
Size: 163 213 27 783 177 291 14 54
Cuts at: 1722 1912 1925 2102 2134 2239 2982 3399 3556
Size: 190 13 177 32 105 743 417 157
Cuts at: 3556 3645
Size: 89
Fragments arranged by size:
783 743 417 291 213 190 177 177
163 157 105 89 54 32 27 14
13
BseYI C'CCAG_C
Cuts at: 0 127 283 469 615 1508 1768 2014 2593
Size: 127 156 186 146 893 260 246 579
Cuts at: 2593 2625 3022 3246 3343 3645
Size: 32 397 224 97 302
Fragments arranged by size:
893 579 397 302 260 246 224 186
156 146 127 97 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2591 3250 3645
Size: 2591 659 395
Fragments arranged by size:
2591 659 395
BsiEI CG_ry'CG
Cuts at: 0 49 3645
Size: 49 3596
BsiHKAI G_wGCw'C
Cuts at: 0 2526 3115 3212 3645
Size: 2526 589 97 433
Fragments arranged by size:
2526 589 433 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1654 2141 2188 2209 2689 3645
Size: 1654 487 47 21 480 956
Fragments arranged by size:
1654 956 487 480 47 21
Bsp1286I G_dGCh'C
Cuts at: 0 119 195 512 545 1097 1186 1199 1232
Size: 119 76 317 33 552 89 13 33
Cuts at: 1232 1853 1886 2126 2526 2714 2980 3021 3115
Size: 621 33 240 400 188 266 41 94
Cuts at: 3115 3212 3310 3402 3645
Size: 97 98 92 243
Fragments arranged by size:
621 552 400 317 266 243 240 188
119 98 97 94 92 89 76 41
33 33 33 13
BspEI T'CCGG_A
Cuts at: 0 1549 2382 3312 3645
Size: 1549 833 930 333
Fragments arranged by size:
1549 930 833 333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 782 1724 2871 3179 3617 3645
Size: 782 942 1147 308 438 28
Fragments arranged by size:
1147 942 782 438 308 28
BsrBI CCG'CTC
Cuts at: 0 321 923 1455 2790 2808 2841 3036 3645
Size: 321 602 532 1335 18 33 195 609
Fragments arranged by size:
1335 609 602 532 321 195 33 18
BsrDI GCAATG_nn'
Cuts at: 0 1875 2794 3645
Size: 1875 919 851
Fragments arranged by size:
1875 919 851
BsrFI r'CCGG_y
Cuts at: 0 687 2230 3645
Size: 687 1543 1415
Fragments arranged by size:
1543 1415 687
BstAPI GCAn_nnn'nTGC
Cuts at: 0 183 3561 3645
Size: 183 3378 84
Fragments arranged by size:
3378 183 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2345 3645
Size: 2345 1300
BstYI r'GATC_y
Cuts at: 0 159 258 1061 3645
Size: 159 99 803 2584
Fragments arranged by size:
2584 803 159 99
Bsu36I CC'TnA_GG
Cuts at: 0 440 2117 2618 3645
Size: 440 1677 501 1027
Fragments arranged by size:
1677 1027 501 440
BtgI C'CryG_G
Cuts at: 0 105 119 383 410 596 999 1045 1814
Size: 105 14 264 27 186 403 46 769
Cuts at: 1814 2852 3574 3645
Size: 1038 722 71
Fragments arranged by size:
1038 769 722 403 264 186 105 71
46 27 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3085 3645
Size: 3085 560
BtsI GCAGTG_nn'
Cuts at: 0 1085 2210 3562 3645
Size: 1085 1125 1352 83
Fragments arranged by size:
1352 1125 1085 83
DrdI GACnn_nn'nnGTC
Cuts at: 0 2275 3645
Size: 2275 1370
EaeI y'GGCC_r
Cuts at: 0 522 1002 1042 2180 2397 2699 2791 2833
Size: 522 480 40 1138 217 302 92 42
Cuts at: 2833 3506 3645
Size: 673 139
Fragments arranged by size:
1138 673 522 480 302 217 139 92
42 40
EarI CTCTTCn'nnn_
Cuts at: 0 1847 2920 3311 3645
Size: 1847 1073 391 334
Fragments arranged by size:
1847 1073 391 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 152 656 859 868 1054 1725 1940 1952
Size: 152 504 203 9 186 671 215 12
Cuts at: 1952 2258 3645
Size: 306 1387
Fragments arranged by size:
1387 671 504 306 215 203 186 152
12 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 217 897 1093 1222 1597 1666 1915
Size: 133 84 680 196 129 375 69 249
Cuts at: 1915 2962 3035 3166 3247 3585 3645
Size: 1047 73 131 81 338 60
Fragments arranged by size:
1047 680 375 338 249 196 133 131
129 84 81 73 69 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3535 3645
Size: 3535 110
EcoO109I rG'GnC_Cy
Cuts at: 0 28 453 509 979 1055 1077 1093 2361
Size: 28 425 56 470 76 22 16 1268
Cuts at: 2361 2622 2976 3141 3528 3645
Size: 261 354 165 387 117
Fragments arranged by size:
1268 470 425 387 354 261 165 117
76 56 28 22 16
HaeII r_GCGC'y
Cuts at: 0 173 217 272 307 470 652 786 897
Size: 173 44 55 35 163 182 134 111
Cuts at: 897 999 1127 1419 1574 2469 3524 3645
Size: 102 128 292 155 895 1055 121
Fragments arranged by size:
1055 895 292 182 173 163 155 134
128 121 111 102 55 44 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 895 927 1258 1290 1354 1386
Size: 144 32 719 32 331 32 64 32
Cuts at: 1386 1581 1613 2237 2269 2989 3021 3645
Size: 195 32 624 32 720 32 624
Fragments arranged by size:
720 719 624 624 331 195 144 64
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 603 3645
Size: 603 3042
KasI G'GCGC_C
Cuts at: 0 169 213 268 303 648 782 995 1123
Size: 169 44 55 35 345 134 213 128
Cuts at: 1123 1570 3520 3645
Size: 447 1950 125
Fragments arranged by size:
1950 447 345 213 169 134 128 125
55 44 35
KpnI G_GTAC'C
Cuts at: 0 2463 3645
Size: 2463 1182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1172 3645
Size: 1172 2473
MscI TGG'CCA
Cuts at: 0 2182 3508 3645
Size: 2182 1326 137
Fragments arranged by size:
2182 1326 137
MspA1I CmG'CkG
Cuts at: 0 9 121 204 412 615 917 1816 1890
Size: 9 112 83 208 203 302 899 74
Cuts at: 1890 1998 2037 2095 2819 3282 3638 3645
Size: 108 39 58 724 463 356 7
Fragments arranged by size:
899 724 463 356 302 208 203 112
108 83 74 58 39 9 7
NaeI GCC'GGC
Cuts at: 0 689 2232 3645
Size: 689 1543 1413
Fragments arranged by size:
1543 1413 689
NarI GG'CG_CC
Cuts at: 0 170 214 269 304 649 783 996 1124
Size: 170 44 55 35 345 134 213 128
Cuts at: 1124 1571 3521 3645
Size: 447 1950 124
Fragments arranged by size:
1950 447 345 213 170 134 128 124
55 44 35
NcoI C'CATG_G
Cuts at: 0 105 383 3645
Size: 105 278 3262
Fragments arranged by size:
3262 278 105
NgoMIV G'CCGG_C
Cuts at: 0 687 2230 3645
Size: 687 1543 1415
Fragments arranged by size:
1543 1415 687
NspI r_CATG'y
Cuts at: 0 3564 3645
Size: 3564 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 3144 3504 3645
Size: 111 3033 360 141
Fragments arranged by size:
3033 360 141 111
PfoI T'CCnGG_A
Cuts at: 0 2258 2483 3645
Size: 2258 225 1162
Fragments arranged by size:
2258 1162 225
PmlI CAC'GTG
Cuts at: 0 2782 3645
Size: 2782 863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 820 852 3645
Size: 379 32 409 32 2793
Fragments arranged by size:
2793 409 379 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 453 979 1077 3528 3645
Size: 453 526 98 2451 117
Fragments arranged by size:
2451 526 453 117 98
PspOMI G'GGCC_C
Cuts at: 0 508 541 1093 2976 3645
Size: 508 33 552 1883 669
Fragments arranged by size:
1883 669 552 508 33
PspXI vC'TCGA_Gb
Cuts at: 0 85 1302 3645
Size: 85 1217 2343
Fragments arranged by size:
2343 1217 85
PstI C_TGCA'G
Cuts at: 0 83 1505 1587 1721 1824 2372 3257 3645
Size: 83 1422 82 134 103 548 885 388
Fragments arranged by size:
1422 885 548 388 134 103 83 82
PvuII CAG'CTG
Cuts at: 0 9 917 3282 3645
Size: 9 908 2365 363
Fragments arranged by size:
2365 908 363 9
SacII CC_GC'GG
Cuts at: 0 122 413 1817 3645
Size: 122 291 1404 1828
Fragments arranged by size:
1828 1404 291 122
SanDI GG'GwC_CC
Cuts at: 0 453 3645
Size: 453 3192
SbfI CC_TGCA'GG
Cuts at: 0 1721 3257 3645
Size: 1721 1536 388
Fragments arranged by size:
1721 1536 388
SexAI A'CCwGG_T
Cuts at: 0 905 3645
Size: 905 2740
SfcI C'TryA_G
Cuts at: 0 79 1501 1583 1717 1820 2368 2963 3253
Size: 79 1422 82 134 103 548 595 290
Cuts at: 3253 3645
Size: 392
Fragments arranged by size:
1422 595 548 392 290 134 103 82
79
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1041 3645
Size: 1041 2604
SfoI GGC'GCC
Cuts at: 0 171 215 270 305 650 784 997 1125
Size: 171 44 55 35 345 134 213 128
Cuts at: 1125 1572 3522 3645
Size: 447 1950 123
Fragments arranged by size:
1950 447 345 213 171 134 128 123
55 44 35
SmaI CCC'GGG
Cuts at: 0 1138 3645
Size: 1138 2507
SmlI C'TyrA_G
Cuts at: 0 85 754 1210 1302 1402 1657 2305 3379
Size: 85 669 456 92 100 255 648 1074
Cuts at: 3379 3645
Size: 266
Fragments arranged by size:
1074 669 648 456 266 255 100 92
85
SphI G_CATG'C
Cuts at: 0 3564 3645
Size: 3564 81
StuI AGG'CCT
Cuts at: 0 1499 3645
Size: 1499 2146
StyI C'CwwG_G
Cuts at: 0 105 299 383 458 701 1232 1805 2897
Size: 105 194 84 75 243 531 573 1092
Cuts at: 2897 3645
Size: 748
Fragments arranged by size:
1092 748 573 531 243 194 105 84
75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 984 1436 1926 2355 3645
Size: 84 900 452 490 429 1290
Fragments arranged by size:
1290 900 490 452 429 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1905 1937 3645
Size: 1905 32 1708
Fragments arranged by size:
1905 1708 32
Tth111I GACn'n_nGTC
Cuts at: 0 907 1696 3645
Size: 907 789 1949
Fragments arranged by size:
1949 907 789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2427 3645
Size: 2427 1218
XhoI C'TCGA_G
Cuts at: 0 85 1302 1657 3645
Size: 85 1217 355 1988
Fragments arranged by size:
1988 1217 355 85
XmaI C'CCGG_G
Cuts at: 0 1136 3645
Size: 1136 2509
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AhdI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI
MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuII SacII SanDI SbfI SexAI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AleI ApaLI AscI
AseI AsiSI AvrII BcgI BclI BfrBI BglII BmgBI
BmtI BsaBI BsiWI BsmI BspHI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI MslI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PsrI
PvuI RsrII SacI SalI SapI ScaI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI XbaI XmnI
ZraI