Restriction map for
pF1KA1193
(Linear) (Six-base) MAPSORT of: KIAA1193.seq Check: 9114 from: 1 to: 1635
KIAA1193.orf
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AfeI AGC'GCT
Cuts at: 0 1045 1635
Size: 1045 590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 440 1635
Size: 268 172 1195
Fragments arranged by size:
1195 268 172
AlwNI CAG_nnn'CTG
Cuts at: 0 810 1125 1299 1635
Size: 810 315 174 336
Fragments arranged by size:
810 336 315 174
AvaI C'yCGr_G
Cuts at: 0 52 189 1588 1635
Size: 52 137 1399 47
Fragments arranged by size:
1399 137 52 47
BamHI G'GATC_C
Cuts at: 0 1473 1635
Size: 1473 162
BanI G'GyrC_C
Cuts at: 0 194 1635
Size: 194 1441
BanII G_rGCy'C
Cuts at: 0 115 896 1241 1607 1635
Size: 115 781 345 366 28
Fragments arranged by size:
781 366 345 115 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 518 674 1635
Size: 518 156 961
Fragments arranged by size:
961 518 156
BbvCI CC'TCA_GC
Cuts at: 0 619 767 1465 1635
Size: 619 148 698 170
Fragments arranged by size:
698 619 170 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 16 50 369 403 1635
Size: 16 34 319 34 1232
Fragments arranged by size:
1232 319 34 34 16
BglII A'GATC_T
Cuts at: 0 653 1635
Size: 653 982
Bme1580I G_kGCm'C
Cuts at: 0 70 199 1582 1635
Size: 70 129 1383 53
Fragments arranged by size:
1383 129 70 53
BmgBI CAC'GTC
Cuts at: 0 333 1548 1635
Size: 333 1215 87
Fragments arranged by size:
1215 333 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1193 1635
Size: 1193 442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 204 509 543 573 910 1635
Size: 30 174 305 34 30 337 725
Fragments arranged by size:
725 337 305 174 34 30 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 241 1434 1635
Size: 137 104 1193 201
Fragments arranged by size:
1193 201 137 104
Bpu10I CC'TnA_GC
Cuts at: 0 619 767 1465 1635
Size: 619 148 698 170
Fragments arranged by size:
698 619 170 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 48 113 1234 1635
Size: 48 65 1121 401
Fragments arranged by size:
1121 401 65 48
BsaAI yAC'GTr
Cuts at: 0 1577 1635
Size: 1577 58
BsaHI Gr'CG_yC
Cuts at: 0 292 1394 1635
Size: 292 1102 241
Fragments arranged by size:
1102 292 241
BsaWI w'CCGG_w
Cuts at: 0 482 1099 1383 1476 1635
Size: 482 617 284 93 159
Fragments arranged by size:
617 482 284 159 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 15 45 220 250 448 478 709 739
Size: 15 30 175 30 198 30 231 30
Cuts at: 739 773 803 1594 1624 1635
Size: 34 30 791 30 11
Fragments arranged by size:
791 231 198 175 34 30 30 30
30 30 30 15 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 196 229 528 565 730 859 928
Size: 3 193 33 299 37 165 129 69
Cuts at: 928 1495 1635
Size: 567 140
Fragments arranged by size:
567 299 193 165 140 129 69 37
33 3
BseYI C'CCAG_C
Cuts at: 0 18 688 1061 1074 1338 1353 1494 1635
Size: 18 670 373 13 264 15 141 141
Fragments arranged by size:
670 373 264 141 141 18 15 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 939 1635
Size: 613 326 696
Fragments arranged by size:
696 613 326
BsiEI CG_ry'CG
Cuts at: 0 1155 1223 1635
Size: 1155 68 412
Fragments arranged by size:
1155 412 68
BsiHKAI G_wGCw'C
Cuts at: 0 59 935 1635
Size: 59 876 700
Fragments arranged by size:
876 700 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 406 1635
Size: 406 1229
Bsp1286I G_dGCh'C
Cuts at: 0 59 70 115 199 896 935 1241 1582
Size: 59 11 45 84 697 39 306 341
Cuts at: 1582 1607 1635
Size: 25 28
Fragments arranged by size:
697 341 306 84 59 45 39 28
25 11
BspEI T'CCGG_A
Cuts at: 0 1099 1383 1476 1635
Size: 1099 284 93 159
Fragments arranged by size:
1099 284 159 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 635 1635
Size: 635 1000
BsrFI r'CCGG_y
Cuts at: 0 638 1222 1635
Size: 638 584 413
Fragments arranged by size:
638 584 413
BsrGI T'GTAC_A
Cuts at: 0 725 1635
Size: 725 910
BssSI C'ACGA_G
Cuts at: 0 457 751 1635
Size: 457 294 884
Fragments arranged by size:
884 457 294
BstEII G'GTnAC_C
Cuts at: 0 1515 1635
Size: 1515 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1458 1635
Size: 1458 177
BstYI r'GATC_y
Cuts at: 0 143 490 653 1197 1473 1635
Size: 143 347 163 544 276 162
Fragments arranged by size:
544 347 276 163 162 143
BtgI C'CryG_G
Cuts at: 0 107 1106 1412 1635
Size: 107 999 306 223
Fragments arranged by size:
999 306 223 107
BtsI GCAGTG_nn'
Cuts at: 0 104 1376 1635
Size: 104 1272 259
Fragments arranged by size:
1272 259 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 80 115 1635
Size: 80 35 1520
Fragments arranged by size:
1520 80 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 691 1635
Size: 691 944
EaeI y'GGCC_r
Cuts at: 0 758 1152 1220 1346 1409 1562 1635
Size: 758 394 68 126 63 153 73
Fragments arranged by size:
758 394 153 126 73 68 63
EagI C'GGCC_G
Cuts at: 0 1152 1220 1635
Size: 1152 68 415
Fragments arranged by size:
1152 415 68
EarI CTCTTCn'nnn_
Cuts at: 0 398 404 478 534 566 905 1635
Size: 398 6 74 56 32 339 730
Fragments arranged by size:
730 398 339 74 56 32 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1635
Size: 19 1616
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 241 1434 1635
Size: 137 104 1193 201
Fragments arranged by size:
1193 201 137 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 970 1429 1635
Size: 970 459 206
Fragments arranged by size:
970 459 206
EcoO109I rG'GnC_Cy
Cuts at: 0 598 684 849 1122 1635
Size: 598 86 165 273 513
Fragments arranged by size:
598 513 273 165 86
FspI TGC'GCA
Cuts at: 0 988 1192 1635
Size: 988 204 443
Fragments arranged by size:
988 443 204
FspAI rTGC'GCAy
Cuts at: 0 988 1192 1635
Size: 988 204 443
Fragments arranged by size:
988 443 204
HaeII r_GCGC'y
Cuts at: 0 811 1047 1635
Size: 811 236 588
Fragments arranged by size:
811 588 236
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 133 165 220 252 403 435
Size: 15 32 86 32 55 32 151 32
Cuts at: 435 1342 1374 1594 1626 1635
Size: 907 32 220 32 9
Fragments arranged by size:
907 220 151 86 55 32 32 32
32 32 32 15 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 566 1019 1635
Size: 491 75 453 616
Fragments arranged by size:
616 491 453 75
MspA1I CmG'CkG
Cuts at: 0 675 1299 1315 1372 1469 1635
Size: 675 624 16 57 97 166
Fragments arranged by size:
675 624 166 97 57 16
NcoI C'CATG_G
Cuts at: 0 107 1635
Size: 107 1528
NspI r_CATG'y
Cuts at: 0 255 1635
Size: 255 1380
PasI CC'CwG_GG
Cuts at: 0 70 1635
Size: 70 1565
PflMI CCAn_nnn'nTGG
Cuts at: 0 1125 1299 1635
Size: 1125 174 336
Fragments arranged by size:
1125 336 174
PmlI CAC'GTG
Cuts at: 0 1577 1635
Size: 1577 58
PpuMI rG'GwC_Cy
Cuts at: 0 598 684 1122 1635
Size: 598 86 438 513
Fragments arranged by size:
598 513 438 86
PshAI GACnn'nnGTC
Cuts at: 0 1263 1635
Size: 1263 372
PspXI vC'TCGA_Gb
Cuts at: 0 52 189 1635
Size: 52 137 1446
Fragments arranged by size:
1446 137 52
PvuII CAG'CTG
Cuts at: 0 675 1299 1372 1635
Size: 675 624 73 263
Fragments arranged by size:
675 624 263 73
SanDI GG'GwC_CC
Cuts at: 0 684 1635
Size: 684 951
SapI GCTCTTCn'nnn_
Cuts at: 0 534 1635
Size: 534 1101
ScaI AGT'ACT
Cuts at: 0 1018 1635
Size: 1018 617
SfcI C'TryA_G
Cuts at: 0 159 1635
Size: 159 1476
SmaI CCC'GGG
Cuts at: 0 1590 1635
Size: 1590 45
SmlI C'TyrA_G
Cuts at: 0 52 189 1635
Size: 52 137 1446
Fragments arranged by size:
1446 137 52
StuI AGG'CCT
Cuts at: 0 10 187 463 1635
Size: 10 177 276 1172
Fragments arranged by size:
1172 276 177 10
StyI C'CwwG_G
Cuts at: 0 107 209 1402 1430 1635
Size: 107 102 1193 28 205
Fragments arranged by size:
1193 205 107 102 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1503 1635
Size: 1503 132
TatI w'GTAC_w
Cuts at: 0 725 1016 1635
Size: 725 291 619
Fragments arranged by size:
725 619 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 448 480 773 805 1635
Size: 448 32 293 32 830
Fragments arranged by size:
830 448 293 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 1126 1404 1635
Size: 216 910 278 231
Fragments arranged by size:
910 278 231 216
XhoI C'TCGA_G
Cuts at: 0 52 189 1635
Size: 52 137 1446
Fragments arranged by size:
1446 137 52
XmaI C'CCGG_G
Cuts at: 0 1588 1635
Size: 1588 47
XmnI GAAnn'nnTTC
Cuts at: 0 229 1555 1635
Size: 229 1326 80
Fragments arranged by size:
1326 229 80
Enzymes that do cut:
AfeI AlfI AlwNI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BsrGI BssSI BstEII BstXI BstYI BtgI BtsI CspCI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I MmeI MspA1I NcoI NspI
PasI PflMI PmlI PpuMI PshAI PspXI PvuII SanDI
SapI ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI
BglI BlpI BmtI BpuEI BsaBI BsiWI BsmI BspHI
BsrBI BsrDI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII EcoICRI EcoRI EcoRV FalI FseI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PsiI PspOMI
PsrI PstI PvuI RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI ZraI