Restriction map for
pF1KA1191
(Linear) (Six-base) MAPSORT of: KIAA1191.seq Check: 1055 from: 1 to: 915
KIAA1191.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 228 915
Size: 228 687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 812 915
Size: 676 136 103
Fragments arranged by size:
676 136 103
AlwNI CAG_nnn'CTG
Cuts at: 0 569 915
Size: 569 346
ApaI G_GGCC'C
Cuts at: 0 867 915
Size: 867 48
ApoI r'AATT_y
Cuts at: 0 318 915
Size: 318 597
AvaI C'yCGr_G
Cuts at: 0 109 915
Size: 109 806
BanII G_rGCy'C
Cuts at: 0 741 867 915
Size: 741 126 48
Fragments arranged by size:
741 126 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 100 134 915
Size: 100 34 781
Fragments arranged by size:
781 100 34
BclI T'GATC_A
Cuts at: 0 326 915
Size: 326 589
BglI GCCn_nnn'nGGC
Cuts at: 0 724 915
Size: 724 191
Bme1580I G_kGCm'C
Cuts at: 0 774 867 915
Size: 774 93 48
Fragments arranged by size:
774 93 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 510 826 915
Size: 277 233 316 89
Fragments arranged by size:
316 277 233 89
Bpu10I CC'TnA_GC
Cuts at: 0 551 915
Size: 551 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 915
Size: 52 863
BsaI GGTCTCn'nnnn_
Cuts at: 0 418 915
Size: 418 497
BsaAI yAC'GTr
Cuts at: 0 769 915
Size: 769 146
BsaBI GATnn'nnATC
Cuts at: 0 826 915
Size: 826 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 415 445 915
Size: 120 30 265 30 470
Fragments arranged by size:
470 265 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 125 436 749 915
Size: 122 3 311 313 166
Fragments arranged by size:
313 311 166 122 3
BsiHKAI G_wGCw'C
Cuts at: 0 899 915
Size: 899 16
BsmI GAATG_Cn'
Cuts at: 0 350 915
Size: 350 565
Bsp1286I G_dGCh'C
Cuts at: 0 741 774 867 899 915
Size: 741 33 93 32 16
Fragments arranged by size:
741 93 33 32 16
BspHI T'CATG_A
Cuts at: 0 314 915
Size: 314 601
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 915
Size: 786 129
BstYI r'GATC_y
Cuts at: 0 685 795 915
Size: 685 110 120
Fragments arranged by size:
685 120 110
BtgI C'CryG_G
Cuts at: 0 620 859 915
Size: 620 239 56
Fragments arranged by size:
620 239 56
EaeI y'GGCC_r
Cuts at: 0 443 915
Size: 443 472
EarI CTCTTCn'nnn_
Cuts at: 0 488 549 915
Size: 488 61 366
Fragments arranged by size:
488 366 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 812 915
Size: 676 136 103
Fragments arranged by size:
676 136 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 915
Size: 202 713
EcoO109I rG'GnC_Cy
Cuts at: 0 114 279 408 915
Size: 114 165 129 507
Fragments arranged by size:
507 165 129 114
EcoRI G'AATT_C
Cuts at: 0 318 915
Size: 318 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 915
Size: 120 32 763
Fragments arranged by size:
763 120 32
MslI CAynn'nnrTG
Cuts at: 0 832 915
Size: 832 83
NcoI C'CATG_G
Cuts at: 0 620 915
Size: 620 295
PasI CC'CwG_GG
Cuts at: 0 563 915
Size: 563 352
PflMI CCAn_nnn'nTGG
Cuts at: 0 569 915
Size: 569 346
PpuMI rG'GwC_Cy
Cuts at: 0 114 279 915
Size: 114 165 636
Fragments arranged by size:
636 165 114
PspOMI G'GGCC_C
Cuts at: 0 863 915
Size: 863 52
PspXI vC'TCGA_Gb
Cuts at: 0 109 915
Size: 109 806
SmlI C'TyrA_G
Cuts at: 0 31 109 915
Size: 31 78 806
Fragments arranged by size:
806 78 31
StuI AGG'CCT
Cuts at: 0 727 915
Size: 727 188
StyI C'CwwG_G
Cuts at: 0 160 203 620 915
Size: 160 43 417 295
Fragments arranged by size:
417 295 160 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 736 915
Size: 696 40 179
Fragments arranged by size:
696 179 40
Tth111I GACn'n_nGTC
Cuts at: 0 249 277 915
Size: 249 28 638
Fragments arranged by size:
638 249 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 915
Size: 267 648
XhoI C'TCGA_G
Cuts at: 0 109 915
Size: 109 806
XmnI GAAnn'nnTTC
Cuts at: 0 350 915
Size: 350 565
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AvaI BanII BcgI
BclI BglI Bme1580I BmrI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BstXI
BstYI BtgI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
Hin4I MslI NcoI PasI PflMI PpuMI PspOMI PspXI
SmlI StuI StyI TaqII Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI BpmI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XbaI XmaI ZraI