Restriction map for
pF1KSDA1187
(Linear) (Six-base) MAPSORT of: KIAA1187.seq Check: 3989 from: 1 to: 2409
KIAA1187.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 2409
Size: 287 2122
AfeI AGC'GCT
Cuts at: 0 628 688 2409
Size: 628 60 1721
Fragments arranged by size:
1721 628 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 593 1334 2239 2409
Size: 593 741 905 170
Fragments arranged by size:
905 741 593 170
AlwNI CAG_nnn'CTG
Cuts at: 0 1215 1600 1627 1962 2119 2409
Size: 1215 385 27 335 157 290
Fragments arranged by size:
1215 385 335 290 157 27
ApaI G_GGCC'C
Cuts at: 0 307 708 1457 2409
Size: 307 401 749 952
Fragments arranged by size:
952 749 401 307
ApaLI G'TGCA_C
Cuts at: 0 1024 2409
Size: 1024 1385
AvaI C'yCGr_G
Cuts at: 0 610 1184 1379 1456 1718 1814 2409
Size: 610 574 195 77 262 96 595
Fragments arranged by size:
610 595 574 262 195 96 77
AvrII C'CTAG_G
Cuts at: 0 229 2409
Size: 229 2180
BanI G'GyrC_C
Cuts at: 0 536 1384 1524 1536 1663 1709 2409
Size: 536 848 140 12 127 46 700
Fragments arranged by size:
848 700 536 140 127 46 12
BanII G_rGCy'C
Cuts at: 0 211 307 708 1265 1441 1457 2183 2409
Size: 211 96 401 557 176 16 726 226
Fragments arranged by size:
726 557 401 226 211 176 96 16
BbeI G_GCGC'C
Cuts at: 0 540 1540 1713 2409
Size: 540 1000 173 696
Fragments arranged by size:
1000 696 540 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 667 2409
Size: 667 1742
BglI GCCn_nnn'nGGC
Cuts at: 0 469 916 1522 2409
Size: 469 447 606 887
Fragments arranged by size:
887 606 469 447
BlpI GC'TnA_GC
Cuts at: 0 754 1264 2409
Size: 754 510 1145
Fragments arranged by size:
1145 754 510
Bme1580I G_kGCm'C
Cuts at: 0 307 708 973 1028 1179 1236 1457 2409
Size: 307 401 265 55 151 57 221 952
Fragments arranged by size:
952 401 307 265 221 151 57 55
BmgBI CAC'GTC
Cuts at: 0 665 2409
Size: 665 1744
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 2409
Size: 228 2181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 502 508 565 607 707 1984 2044
Size: 68 434 6 57 42 100 1277 60
Cuts at: 2044 2409
Size: 365
Fragments arranged by size:
1277 434 365 100 68 60 57 42
6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 2350 2409
Size: 1183 1167 59
Fragments arranged by size:
1183 1167 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 1579 2409
Size: 1579 830
BsaAI yAC'GTr
Cuts at: 0 893 1231 2409
Size: 893 338 1178
Fragments arranged by size:
1178 893 338
BsaHI Gr'CG_yC
Cuts at: 0 537 793 1000 1537 1710 2409
Size: 537 256 207 537 173 699
Fragments arranged by size:
699 537 537 256 207 173
BsaWI w'CCGG_w
Cuts at: 0 1092 2409
Size: 1092 1317
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1344 1374 2125 2155 2185 2215 2409
Size: 1344 30 751 30 30 30 194
Fragments arranged by size:
1344 751 194 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 314 505 562 1438 1753 1801 1864 1903
Size: 314 191 57 876 315 48 63 39
Cuts at: 1903 1933 2041 2191 2409
Size: 30 108 150 218
Fragments arranged by size:
876 315 314 218 191 150 108 63
57 48 39 30
BseYI C'CCAG_C
Cuts at: 0 24 42 1333 1441 1512 1551 1620 2259
Size: 24 18 1291 108 71 39 69 639
Cuts at: 2259 2409
Size: 150
Fragments arranged by size:
1291 639 150 108 71 69 39 24
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 694 1026 1144 2188 2401 2409
Size: 599 95 332 118 1044 213 8
Fragments arranged by size:
1044 599 332 213 118 95 8
BsiEI CG_ry'CG
Cuts at: 0 852 939 1108 1936 2409
Size: 852 87 169 828 473
Fragments arranged by size:
852 828 473 169 87
BsiHKAI G_wGCw'C
Cuts at: 0 1028 1265 2409
Size: 1028 237 1144
Fragments arranged by size:
1144 1028 237
BsmI GAATG_Cn'
Cuts at: 0 174 1784 2409
Size: 174 1610 625
Fragments arranged by size:
1610 625 174
Bsp1286I G_dGCh'C
Cuts at: 0 211 307 708 973 1028 1179 1236 1265
Size: 211 96 401 265 55 151 57 29
Cuts at: 1265 1441 1457 2183 2409
Size: 176 16 726 226
Fragments arranged by size:
726 401 265 226 211 176 151 96
57 55 29 16
BspHI T'CATG_A
Cuts at: 0 2033 2409
Size: 2033 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 2382 2409
Size: 210 2172 27
Fragments arranged by size:
2172 210 27
BsrBI CCG'CTC
Cuts at: 0 8 432 444 1090 1152 1749 1824 1899
Size: 8 424 12 646 62 597 75 75
Cuts at: 1899 1953 1968 2001 2409
Size: 54 15 33 408
Fragments arranged by size:
646 597 424 408 75 75 62 54
33 15 12 8
BsrFI r'CCGG_y
Cuts at: 0 533 539 963 1042 1103 1119 1521 1533
Size: 533 6 424 79 61 16 402 12
Cuts at: 1533 1660 2409
Size: 127 749
Fragments arranged by size:
749 533 424 402 127 79 61 16
12 6
BssHII G'CGCG_C
Cuts at: 0 919 2409
Size: 919 1490
BssSI C'ACGA_G
Cuts at: 0 1862 2409
Size: 1862 547
BstEII G'GTnAC_C
Cuts at: 0 91 1638 2409
Size: 91 1547 771
Fragments arranged by size:
1547 771 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 2275 2409
Size: 456 1819 134
Fragments arranged by size:
1819 456 134
Bsu36I CC'TnA_GG
Cuts at: 0 213 2409
Size: 213 2196
BtgI C'CryG_G
Cuts at: 0 299 307 1580 2409
Size: 299 8 1273 829
Fragments arranged by size:
1273 829 299 8
BtsI GCAGTG_nn'
Cuts at: 0 362 479 835 1388 2409
Size: 362 117 356 553 1021
Fragments arranged by size:
1021 553 362 356 117
DrdI GACnn_nn'nnGTC
Cuts at: 0 1603 2049 2409
Size: 1603 446 360
Fragments arranged by size:
1603 446 360
EaeI y'GGCC_r
Cuts at: 0 461 849 1105 1658 2409
Size: 461 388 256 553 751
Fragments arranged by size:
751 553 461 388 256
EagI C'GGCC_G
Cuts at: 0 849 1105 2409
Size: 849 256 1304
Fragments arranged by size:
1304 849 256
EarI CTCTTCn'nnn_
Cuts at: 0 245 434 1165 1937 2032 2409
Size: 245 189 731 772 95 377
Fragments arranged by size:
772 731 377 245 189 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 975 1233 1834 1846 1963 2409
Size: 975 258 601 12 117 446
Fragments arranged by size:
975 601 446 258 117 12
EcoICRI GAG'CTC
Cuts at: 0 1263 2409
Size: 1263 1146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 287 502 508 565 607 707 1984
Size: 68 219 215 6 57 42 100 1277
Cuts at: 1984 2044 2409
Size: 60 365
Fragments arranged by size:
1277 365 219 215 100 68 60 57
42 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 881 1311 2409
Size: 881 430 1098
Fragments arranged by size:
1098 881 430
EcoO109I rG'GnC_Cy
Cuts at: 0 59 283 704 705 886 1453 2409
Size: 59 224 421 1 181 567 956
Fragments arranged by size:
956 567 421 224 181 59 1
HaeII r_GCGC'y
Cuts at: 0 508 534 540 630 690 1062 1209 1448
Size: 508 26 6 90 60 372 147 239
Cuts at: 1448 1540 1713 2409
Size: 92 173 696
Fragments arranged by size:
696 508 372 239 173 147 92 90
60 26 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 783 815 1486 1518 2125 2157 2409
Size: 783 32 671 32 607 32 252
Fragments arranged by size:
783 671 607 252 32 32 32
KasI G'GCGC_C
Cuts at: 0 536 1536 1709 2409
Size: 536 1000 173 700
Fragments arranged by size:
1000 700 536 173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 392 669 2409
Size: 373 19 277 1740
Fragments arranged by size:
1740 373 277 19
MspA1I CmG'CkG
Cuts at: 0 46 600 753 1031 1582 1728 1794 2409
Size: 46 554 153 278 551 146 66 615
Fragments arranged by size:
615 554 551 278 153 146 66 46
NaeI GCC'GGC
Cuts at: 0 535 541 1105 1535 1662 2409
Size: 535 6 564 430 127 747
Fragments arranged by size:
747 564 535 430 127 6
NarI GG'CG_CC
Cuts at: 0 537 1537 1710 2409
Size: 537 1000 173 699
Fragments arranged by size:
1000 699 537 173
NcoI C'CATG_G
Cuts at: 0 299 2409
Size: 299 2110
NgoMIV G'CCGG_C
Cuts at: 0 533 539 1103 1533 1660 2409
Size: 533 6 564 430 127 749
Fragments arranged by size:
749 564 533 430 127 6
PasI CC'CwG_GG
Cuts at: 0 877 1339 2146 2409
Size: 877 462 807 263
Fragments arranged by size:
877 807 462 263
PflMI CCAn_nnn'nTGG
Cuts at: 0 1307 2409
Size: 1307 1102
PfoI T'CCnGG_A
Cuts at: 0 524 723 2409
Size: 524 199 1686
Fragments arranged by size:
1686 524 199
PmlI CAC'GTG
Cuts at: 0 893 1231 2409
Size: 893 338 1178
Fragments arranged by size:
1178 893 338
PpuMI rG'GwC_Cy
Cuts at: 0 59 283 2409
Size: 59 224 2126
Fragments arranged by size:
2126 224 59
PshAI GACnn'nnGTC
Cuts at: 0 2309 2409
Size: 2309 100
PspOMI G'GGCC_C
Cuts at: 0 303 704 1453 2409
Size: 303 401 749 956
Fragments arranged by size:
956 749 401 303
PstI C_TGCA'G
Cuts at: 0 752 957 1760 1907 2156 2252 2409
Size: 752 205 803 147 249 96 157
Fragments arranged by size:
803 752 249 205 157 147 96
PvuII CAG'CTG
Cuts at: 0 46 753 1794 2409
Size: 46 707 1041 615
Fragments arranged by size:
1041 707 615 46
SacI G_AGCT'C
Cuts at: 0 1265 2409
Size: 1265 1144
SacII CC_GC'GG
Cuts at: 0 1583 2409
Size: 1583 826
SapI GCTCTTCn'nnn_
Cuts at: 0 434 2409
Size: 434 1975
SfcI C'TryA_G
Cuts at: 0 748 953 1592 1756 1903 2152 2248 2409
Size: 748 205 639 164 147 249 96 161
Fragments arranged by size:
748 639 249 205 164 161 147 96
SfoI GGC'GCC
Cuts at: 0 538 1538 1711 2409
Size: 538 1000 173 698
Fragments arranged by size:
1000 698 538 173
SgrAI Cr'CCGG_yG
Cuts at: 0 533 1042 1119 1533 2409
Size: 533 509 77 414 876
Fragments arranged by size:
876 533 509 414 77
SmaI CCC'GGG
Cuts at: 0 1186 1720 1816 2409
Size: 1186 534 96 593
Fragments arranged by size:
1186 593 534 96
SmlI C'TyrA_G
Cuts at: 0 610 1198 2365 2409
Size: 610 588 1167 44
Fragments arranged by size:
1167 610 588 44
SrfI GCCC'GGGC
Cuts at: 0 1816 2409
Size: 1816 593
StuI AGG'CCT
Cuts at: 0 1114 1317 2409
Size: 1114 203 1092
Fragments arranged by size:
1114 1092 203
StyI C'CwwG_G
Cuts at: 0 229 299 500 1277 1400 2409
Size: 229 70 201 777 123 1009
Fragments arranged by size:
1009 777 229 201 123 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1688 1922 2129 2409
Size: 1688 234 207 280
Fragments arranged by size:
1688 280 234 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1344 1376 2185 2217 2409
Size: 1344 32 809 32 192
Fragments arranged by size:
1344 809 192 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1692 2409
Size: 1692 717
XhoI C'TCGA_G
Cuts at: 0 610 2409
Size: 610 1799
XmaI C'CCGG_G
Cuts at: 0 1184 1718 1814 2409
Size: 1184 534 96 595
Fragments arranged by size:
1184 595 534 96
XmnI GAAnn'nnTTC
Cuts at: 0 2278 2409
Size: 2278 131
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI AvrII
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MspA1I
NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI
SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI
BmtI Bpu10I BsaBI BsiWI BsmBI BspEI BsrDI BsrGI
BstAPI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MslI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SspI SwaI TatI Tth111I XbaI ZraI