Restriction map for
pF1KSDA1186
(Linear) (Six-base) MAPSORT of: KIAA1186.seq Check: 9433 from: 1 to: 1209
KIAA1186.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1069 1209
Size: 1069 140
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 979 1064 1209
Size: 577 402 85 145
Fragments arranged by size:
577 402 145 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 419 1209
Size: 419 790
AlwNI CAG_nnn'CTG
Cuts at: 0 249 357 1209
Size: 249 108 852
Fragments arranged by size:
852 249 108
BanI G'GyrC_C
Cuts at: 0 708 727 1198 1209
Size: 708 19 471 11
Fragments arranged by size:
708 471 19 11
BanII G_rGCy'C
Cuts at: 0 28 1209
Size: 28 1181
BbsI GAAGACnn'nnnn_
Cuts at: 0 967 1209
Size: 967 242
BbvCI CC'TCA_GC
Cuts at: 0 361 1209
Size: 361 848
Bme1580I G_kGCm'C
Cuts at: 0 408 730 1209
Size: 408 322 479
Fragments arranged by size:
479 408 322
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 1209
Size: 283 926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 1209
Size: 538 671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1209
Size: 212 997
Bpu10I CC'TnA_GC
Cuts at: 0 361 1209
Size: 361 848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1209
Size: 463 746
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 806 1209
Size: 433 373 403
Fragments arranged by size:
433 403 373
BsaWI w'CCGG_w
Cuts at: 0 167 1209
Size: 167 1042
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 923 953 1209
Size: 923 30 256
Fragments arranged by size:
923 256 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 852 1209
Size: 852 357
BseYI C'CCAG_C
Cuts at: 0 1188 1209
Size: 1188 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1209
Size: 865 344
Bsp1286I G_dGCh'C
Cuts at: 0 28 408 730 1209
Size: 28 380 322 479
Fragments arranged by size:
479 380 322 28
BspEI T'CCGG_A
Cuts at: 0 167 1209
Size: 167 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 365 1069 1209
Size: 152 213 704 140
Fragments arranged by size:
704 213 152 140
BsrBI CCG'CTC
Cuts at: 0 5 934 1209
Size: 5 929 275
Fragments arranged by size:
929 275 5
BsrFI r'CCGG_y
Cuts at: 0 81 1209
Size: 81 1128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 423 1209
Size: 423 786
BstYI r'GATC_y
Cuts at: 0 884 1209
Size: 884 325
Bsu36I CC'TnA_GG
Cuts at: 0 907 1209
Size: 907 302
BtgI C'CryG_G
Cuts at: 0 855 1209
Size: 855 354
BtsI GCAGTG_nn'
Cuts at: 0 1071 1209
Size: 1071 138
DraIII CAC_nnn'GTG
Cuts at: 0 843 873 1209
Size: 843 30 336
Fragments arranged by size:
843 336 30
EaeI y'GGCC_r
Cuts at: 0 47 1209
Size: 47 1162
EarI CTCTTCn'nnn_
Cuts at: 0 895 1166 1209
Size: 895 271 43
Fragments arranged by size:
895 271 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 577 979 1064 1209
Size: 212 365 402 85 145
Fragments arranged by size:
402 365 212 145 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 1209
Size: 628 581
EcoO109I rG'GnC_Cy
Cuts at: 0 354 1052 1146 1209
Size: 354 698 94 63
Fragments arranged by size:
698 354 94 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1182 1209
Size: 1150 32 27
Fragments arranged by size:
1150 32 27
HindIII A'AGCT_T
Cuts at: 0 135 1209
Size: 135 1074
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 264 1209
Size: 134 130 945
Fragments arranged by size:
945 134 130
MscI TGG'CCA
Cuts at: 0 49 1209
Size: 49 1160
MspA1I CmG'CkG
Cuts at: 0 984 1188 1209
Size: 984 204 21
Fragments arranged by size:
984 204 21
NaeI GCC'GGC
Cuts at: 0 83 1209
Size: 83 1126
NgoMIV G'CCGG_C
Cuts at: 0 81 1209
Size: 81 1128
NspI r_CATG'y
Cuts at: 0 178 531 1209
Size: 178 353 678
Fragments arranged by size:
678 353 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 873 1209
Size: 873 336
PfoI T'CCnGG_A
Cuts at: 0 68 1209
Size: 68 1141
PpuMI rG'GwC_Cy
Cuts at: 0 354 1052 1146 1209
Size: 354 698 94 63
Fragments arranged by size:
698 354 94 63
PstI C_TGCA'G
Cuts at: 0 149 1066 1209
Size: 149 917 143
Fragments arranged by size:
917 149 143
PvuII CAG'CTG
Cuts at: 0 1188 1209
Size: 1188 21
SanDI GG'GwC_CC
Cuts at: 0 1052 1209
Size: 1052 157
SexAI A'CCwGG_T
Cuts at: 0 704 815 1034 1209
Size: 704 111 219 175
Fragments arranged by size:
704 219 175 111
SfcI C'TryA_G
Cuts at: 0 145 1062 1104 1209
Size: 145 917 42 105
Fragments arranged by size:
917 145 105 42
SmlI C'TyrA_G
Cuts at: 0 478 1209
Size: 478 731
StuI AGG'CCT
Cuts at: 0 508 1209
Size: 508 701
StyI C'CwwG_G
Cuts at: 0 323 1209
Size: 323 886
Enzymes that do cut:
AarI AcuI AlfI AlwNI BanI BanII BbsI BbvCI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrBI
BsrFI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I Hin4I HindIII MmeI MscI
MspA1I NaeI NgoMIV NspI PflMI PfoI PpuMI PstI
PvuII SanDI SexAI SfcI SmlI StuI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaAI
BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MslI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI